| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 813,484 | T→G | 36.0% | S202S (TCA→TCC) | prsA ← | phosphoribosylpyrophosphate synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 813,484 | 0 | T | G | 36.0% | 105.6 / 131.1 | 152 | S202S (TCA→TCC) | prsA | phosphoribosylpyrophosphate synthase |
| Reads supporting (aligned to +/- strand): ref base T (68/29); new base G (29/25); total (98/54) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.23e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.52e-01 | |||||||||||
Only 100 of 164 total aligned reads displayed.
GTCGATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTGTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGAT > minE/813418‑813552 | gtCGATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGa > 1:1338953/1‑69 (MQ=255) tCGATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGaa > 1:869557/1‑69 (MQ=255) tCGATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGaa > 1:587633/1‑69 (MQ=255) tCGATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGaa > 1:202874/1‑69 (MQ=255) gATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGGGaaa > 1:2758680/1‑68 (MQ=255) gATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGGGAAAc > 1:1789708/1‑69 (MQ=255) gATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGGGAAAc > 1:2656469/1‑69 (MQ=255) gATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGGGAAAc > 1:2356442/1‑69 (MQ=255) gATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGGGAAAc > 1:2545982/1‑69 (MQ=255) gATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGGGAAAc > 1:269186/1‑69 (MQ=255) gATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGGGAAAc > 1:273224/1‑69 (MQ=255) gATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGGGAAAc > 1:2881791/1‑69 (MQ=255) gATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGGGAAAc > 1:2948337/1‑69 (MQ=255) gATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGGGAAAc > 1:2972052/1‑69 (MQ=255) gATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGGGAAAc > 1:3008316/1‑69 (MQ=255) gATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGGGAAAc > 1:3147822/1‑69 (MQ=255) gATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGGGAAAc > 1:316667/1‑69 (MQ=255) gATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGGGAAAc > 1:36439/1‑69 (MQ=255) gATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGGGAAAc > 1:482009/1‑69 (MQ=255) gATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGGGAAAc > 1:966943/1‑69 (MQ=255) gATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGGGAAAc > 1:1139422/1‑69 (MQ=255) gATCATATCATCGACCAGTACGCAGTCACAACCTGCAACGTCACCGATGATATGCATCACCTGGGAAAc > 1:2830879/1‑69 (MQ=255) atcatcGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGc > 1:2673136/1‑69 (MQ=255) atcatcGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAAAGTTCGc > 1:41849/1‑69 (MQ=255) cGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCAcgcg > 1:2126212/1‑69 (MQ=255) cAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTg > 1:1044481/1‑69 (MQ=255) cAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTg > 1:1038150/1‑69 (MQ=255) cAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTg > 1:2477474/1‑69 (MQ=255) cAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTg > 1:345777/1‑69 (MQ=255) cAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTg > 1:2238176/1‑69 (MQ=255) cAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTg > 1:944407/1‑69 (MQ=255) cACGCCCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGgac > 1:3139261/1‑57 (MQ=255) cACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTGTCg > 1:2312585/1‑68 (MQ=255) cACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTGTCg > 1:891465/1‑68 (MQ=255) cACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTGTCg > 1:32215/1‑68 (MQ=255) cACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTGTCg > 1:3249813/1‑68 (MQ=255) cACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTGTCg > 1:1278803/1‑68 (MQ=255) cACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTGTCg > 1:344057/1‑68 (MQ=255) cACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTGTCg > 1:1789287/1‑68 (MQ=255) cACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTGTCg > 1:2264700/1‑68 (MQ=255) cACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTGTCg > 1:2130065/1‑68 (MQ=255) cACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTGTCg > 1:662958/1‑68 (MQ=255) cACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGATCGCAcg > 1:3042044/1‑52 (MQ=255) aCGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTGTCgat > 1:1603366/1‑69 (MQ=255) aCGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTGTCgat > 1:705965/1‑69 (MQ=255) aCGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTGTCgat > 1:175757/1‑69 (MQ=255) aCGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTGTCgat > 1:2581733/1‑69 (MQ=255) cTGCAACGTCACCGATGATATGCATCACCTGGGAAACGt < 1:3423961/39‑1 (MQ=255) cTGCAACGTCACCGATGATATGCATCACCTGGGAAACGt < 1:1367410/39‑1 (MQ=255) cTGCAACGTCACCGATGATATGCATCACCTGGGAAACGt < 1:2915954/39‑1 (MQ=255) cTGCAACGTCACCGATGATATGCATCACCTGGGAAACGt < 1:2929009/39‑1 (MQ=255) cTGCAACGTCACCGATGATATGCATCACCTGGGAAACGt < 1:552756/39‑1 (MQ=255) cTGCAACGTCACCGATGATATGCATCACCTGGGAAACGt < 1:343272/39‑1 (MQ=255) cTGCAACGTCACCGATGATATGCATCACCTGGGAAACGt < 1:854478/39‑1 (MQ=255) cTGCAACGTCACCGATGATATGCATCACCTGGGAAACGt < 1:4876/39‑1 (MQ=255) cTGCAACGTCACCGATGATATGCATCACCTGGGAAACGt < 1:548758/39‑1 (MQ=255) cTGCAACGTCACCGATGATATGCATCACCTGGGAAACGt < 1:2375057/39‑1 (MQ=255) cTGCAACGTCACCGATGATATGCATCACCTGGGAAACGt < 1:2423069/39‑1 (MQ=255) cTGCAACGTCACCGATGATATGCATCACCTGGGAAACGt < 1:199725/39‑1 (MQ=255) cTGCAACGTCACCGATGATATGCATCACCTGGGAAACGt < 1:2659493/39‑1 (MQ=255) cTGAAACGTCACCGATGATATGCATCACCTGGGAAACGt < 1:408875/39‑1 (MQ=37) gCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTGTc > 1:1660567/1‑59 (MQ=255) gCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTGTc > 1:1104826/1‑59 (MQ=255) gCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTGTc > 1:2097751/1‑59 (MQ=255) aCGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTGTCGATGATTGCCat < 1:1113780/68‑1 (MQ=255) aCGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTGTCGATGATTGCCat < 1:1191982/68‑1 (MQ=255) aCGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTGTCGATGATTGCCat < 1:20834/68‑1 (MQ=255) aCGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTGTCGATGATTGCCat < 1:1586241/68‑1 (MQ=255) aCGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTGTCGATGATTGCCat < 1:3080706/68‑1 (MQ=255) aCGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTGTCGATGATTGCCat < 1:1862688/68‑1 (MQ=255) aCGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTGTCGATGATTGCCat < 1:1418738/68‑1 (MQ=255) gTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTGTCGATGATTGCCat < 1:1414608/66‑1 (MQ=255) cATCACCTGTGAAACGTTCGCACGCGGACGACGTTTGTCGATGATTGCCATATCGGTATCGTTcagcag > 1:1427184/1‑69 (MQ=255) aCCTGTGAAACGTTCGCACGCGGACGACGTTTGTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTa > 1:1646777/1‑69 (MQ=255) aCCTGTGAAACGTTCGCACGCGGACGACGTTTGTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTa > 1:2335739/1‑69 (MQ=255) aCCTGTGAAACGTTCGCACGCGGACGACGTTTGTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTa > 1:729481/1‑69 (MQ=255) aCCTGTGAAACGTTCGCACGCGGACGACGTTTGTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTa > 1:692765/1‑69 (MQ=255) aCCTGTGAAACGTTCGCACGCGGACGACGTTTGTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTa > 1:457721/1‑69 (MQ=255) aCCTGTGAAACGTTCGCACGCGGACGACGTTTGTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTa > 1:2196784/1‑69 (MQ=255) aCCTGTGAAACGTTCGCACGCGGACGACGTTTGTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTa > 1:1292003/1‑69 (MQ=255) aCCTGTGAAACGTTCGCACGCGGACGACGTTTGTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTa > 1:502956/1‑69 (MQ=255) cTGTGAAACGTTCGCACGCGGACGACGTTTGTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGc < 1:339213/69‑1 (MQ=255) cTGTGAAACGTTCGCACGCGGACGACGTTTGTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGc < 1:1470451/69‑1 (MQ=255) cTGTGAAACGTTCGCACGCGGACGACGTTTGTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGc < 1:1673702/69‑1 (MQ=255) cTGTGAAACGTTCGCACGCGGACGACGTTTGTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGc < 1:2667493/69‑1 (MQ=255) cTGTGAAACGTTCGCACGCGGACGACGTTTGTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGc < 1:1878549/69‑1 (MQ=255) cTGTGAAACGTTCGCACGCGGACGACGTTTGTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGc < 1:2564375/69‑1 (MQ=255) cTGTGAAACGTTCGCACGCGGACGACGTTTGTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGc < 1:1202111/69‑1 (MQ=255) cTGTGAAACGTTCGCACGCGGACGACGTTTGTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGc < 1:1523841/69‑1 (MQ=255) cTGTGAAACGTTCGCACGCGGACGACGTTTGTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGc < 1:2294472/69‑1 (MQ=255) cTGTGAAACGTTCGCACGCGGACGACGTTTGTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGc < 1:157245/69‑1 (MQ=255) cTGTGAAACGTTCGCACGCGGACGACGTTTGTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGc < 1:3355119/69‑1 (MQ=255) cTGTGAAACGTTCGCACGCGGACGACGTTTGTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGc < 1:3388406/69‑1 (MQ=255) tgtgAAACGTTCGCACGCGGACGACGTTTGTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGc < 1:1265117/68‑1 (MQ=255) gtgAAACGTTCGCACGCGGACGACGTTTGTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGc > 1:2562597/1‑67 (MQ=255) gtgAAACGTTCGCACGCGGACGACGTTTGTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGc > 1:3285942/1‑67 (MQ=255) gtgAAACGTTCGCACGCGGACGACGTTTGTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGa < 1:1716676/69‑1 (MQ=255) tgAAACGTTCGCACGCGGACGACGTTTGTCGATGATTGCCATATCGGTa > 1:2261199/1‑49 (MQ=255) tgAAACGTTCGCACGCGGACGACGTTTGTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGAt > 1:578559/1‑69 (MQ=255) tgAAACGTTCGCACGCGGACGACGTTTGTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGAt > 1:783625/1‑69 (MQ=255) | GTCGATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTGTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGAT > minE/813418‑813552 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |