| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 817,435 | T→C | 36.9% | D70D (GAT→GAC) | prfA → | peptide chain release factor RF‑1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 817,435 | 0 | T | C | 36.9% | 70.0 / 75.5 | 119 | D70D (GAT→GAC) | prfA | peptide chain release factor RF‑1 |
| Reads supporting (aligned to +/- strand): ref base T (28/47); new base C (8/36); total (36/83) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.82e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.21e-01 | |||||||||||
Only 100 of 122 total aligned reads displayed.
CTGTTTTACCGACTGGCAACAGGTTCAGGAAGATATCGAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGG > minE/817375‑817502 | cTGTTTTACCGACTGGCAACAGGTTCAGGAAGATATCGAAACCGCTCAGATGATGCTCGACGATCCTGa < 1:2662384/69‑1 (MQ=255)cTGTTTTACCGACTGGCAACAGGTTCAGGAAGATATCGAAACCGCACAGATGATGCTCGATGATCCTGa < 1:903987/69‑1 (MQ=255)cTGTTTTACCGACTGGCAACAGGTTCAGGAAGATATCGAAACCGCACAGATGATGCTCGATGATCCTGa < 1:808893/69‑1 (MQ=255)cTGTTTTACCGACTGGCAACAGGTTCAGGAAGATATCGAAACCGCACAGATGATGCTCGATGATCCTGa < 1:2153266/69‑1 (MQ=255)cTGTTTTACCGACTGGCAACAGGTTCAGGAAGATATCGAAACCGCACAGATGATGCTCGATGATCCTGa < 1:2945916/69‑1 (MQ=255)cTGTTTTACCGACTGGCAACAGGTTCAGGAAGATATCGAAACCGCACAGATGATGCTCGATGATCCTGa < 1:3075161/69‑1 (MQ=255)cTGTTTTACCGACTGGCAACAGGTTCAGGAAGATATCGAAACCGCACAGATGATGCTCGACGATCCTGa < 1:2137582/69‑1 (MQ=255)cTGTTTTACCGACTGGCAACAGGTTCAGGAAGATATCGAAACCGCACAGATGATGCTCGACGATCCTGa < 1:2140487/69‑1 (MQ=255)cTGTTTTACCGACTGGCAACAGGTTCAGGAAGATATCGAAACCGCACAGATGATGCTCGACGATCCTGa < 1:2055166/69‑1 (MQ=255)cTGTTTTACCGACTGGCAACAGGTTCAGGAAGATATCGAAACCGCACAGATGATGCTCGACGATCCTGa < 1:2578725/69‑1 (MQ=255)cTGTTTTACCGACTGGCAACAGGTTCAGGAAGATATCGAAACCGCACAGATGATGCTCGACGATCCTGa < 1:263663/69‑1 (MQ=255)cTGTTTTACCGACTGGCAACAGGTTCAGGAAGATATCGAAACCGCACAGATGATGCTCGACGATCCTGa < 1:1581511/69‑1 (MQ=255)cTGTTTTACCGACTGGCAACAGGTTCAGGAAGATATCGAAACCGCACAGATGATGCTCGACGATCCTGa < 1:3342929/69‑1 (MQ=255)cTGTTTTACCGACTGGCAACAGGTTCAGGAAGATATCGAAACCGCACAGATGATGCTCGACGATCCTGa < 1:373790/69‑1 (MQ=255)cTGTTTTACCGACTGGCAACAGGTTCAGGAAGATATCGAAACCGCACAGATGATGCTCGACGATCCTGa < 1:799851/69‑1 (MQ=255)cTGTTTTACCGACTGGCAACAGGTTCAGGAAGATATCGAAACCGCACAGATGATGCTCGACGATCCTGa < 1:442705/69‑1 (MQ=255)cTGTTTTACCGACTGGCAACAGGTTCAGGAAGATATCGAAACCGCACAGATGATGCTCGACGATCCTGa < 1:692862/69‑1 (MQ=255) tGTTTTACCGACTGGCAACAGGTTCAGGAAGATATCGAAACCGCACAGATGATGCTCGATGATCCTGa < 1:1569639/68‑1 (MQ=255) gCAACAGGTTCAGGAAGATATCGAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTga > 1:3353357/1‑63 (MQ=255) gCAACAGGTTCAGGAAGATATAGAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTga > 1:2788263/1‑63 (MQ=255) ttCAGGAAGATATCGAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATg < 1:1045175/69‑1 (MQ=255) ttCAGGAAGATATCGAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATg < 1:2683527/69‑1 (MQ=255) gAAGATATCGAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTg < 1:2845085/69‑1 (MQ=255) gAAGATATCGAAACCGCACAGATGATGCTCGACGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTg < 1:972262/69‑1 (MQ=255) gAAGATATCGAAACCGCACAGATGATGCTCGACGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTg < 1:2358678/69‑1 (MQ=255) gAAGATATCGAAACCGCACAGATGATGCTCGACGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTg < 1:768584/69‑1 (MQ=255) gAAGATATCGAAACCGCACAGATGATGCTCGACGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTg < 1:2387392/69‑1 (MQ=255) gAAGATATCGAAACCGCACAGATGATGCTCGACGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTg < 1:2575815/69‑1 (MQ=255) gAAGATATCGAAACCGCACAGATGATGCTCGACGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTg < 1:672414/69‑1 (MQ=255) gAAGATATCGAAACCGCACAGATGATGCTCGACGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTg < 1:2853219/69‑1 (MQ=255) gAAGATATCGAAACCGCACAGATGATGCTCGACGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTg < 1:399397/69‑1 (MQ=255) gAAGATATCGAAACCGCACAGATGATGCTCGACGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTg < 1:3067172/69‑1 (MQ=255) gAAGATATCGAAACCGCACAGATGATGCTCGACGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTg < 1:3130503/69‑1 (MQ=255) gAAGATATCGAAACCGCACAGATGATGCTCGACGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTg < 1:1186842/69‑1 (MQ=255) gAAGATATCGAAACCGCACAGATGATGCTCGACGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTg < 1:1524411/69‑1 (MQ=255) gAAGATATCGAAACCGCACAGATGATGCTCGACGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTg < 1:1529481/69‑1 (MQ=255) gAAGATATCGAAACCGCACAGATGATGCTCGACGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTg < 1:1550912/69‑1 (MQ=255) aaGATATCGAAACCGCACAGATGATGCTCGACGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTg < 1:3065529/68‑1 (MQ=255) aaGATATCGAAACCGCACAGATGATGCTCGACGATCCTGAAATGCGTGAGATCGCGCAGGATGAACTg < 1:3133367/68‑1 (MQ=255) aGATATCGAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATg < 1:1054113/62‑1 (MQ=255) aGATATCGAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATg < 1:769050/62‑1 (MQ=255) aGATATCGAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATg < 1:1461765/62‑1 (MQ=255) aGATATCGAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATg < 1:280706/62‑1 (MQ=255) aGATATCGAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATg < 1:2081704/62‑1 (MQ=255) aGATATCGAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATg < 1:238825/62‑1 (MQ=255) atatCGAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGcgcg < 1:351885/69‑1 (MQ=255) atatCGAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGcgcg < 1:1274191/69‑1 (MQ=255) atatCGAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGcgcg < 1:3233761/69‑1 (MQ=255) atatCGAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGcgcg < 1:1853658/69‑1 (MQ=255) atatCGAAACCGCACAGATGATGCTCGACGATCCTGa < 1:740647/37‑1 (MQ=255) tatCGAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGa < 1:3014912/69‑1 (MQ=255) tatCGAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGa < 1:3012188/69‑1 (MQ=255) tatCGAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGa > 1:2008554/1‑69 (MQ=255) tatCGAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGa < 1:1186089/69‑1 (MQ=255) tatCGAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGa < 1:3301938/69‑1 (MQ=255) tatCGAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGa < 1:2643742/69‑1 (MQ=255) tatCGAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGa > 1:3305161/1‑69 (MQ=255) tatCGAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGa < 1:3337347/69‑1 (MQ=255) tatCGAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGa < 1:67195/69‑1 (MQ=255) tatCGAAACCGCACAGATGATGATCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGa > 1:2160115/1‑69 (MQ=255) atCGAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGa < 1:525107/68‑1 (MQ=255) gAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCt < 1:1426760/69‑1 (MQ=255) gAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCt < 1:1320835/69‑1 (MQ=255) gAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCt < 1:2078572/69‑1 (MQ=255) gAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCt < 1:115989/69‑1 (MQ=255) gAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCt < 1:1996942/69‑1 (MQ=255) gAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCt < 1:2596371/69‑1 (MQ=255) gAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCt < 1:2610073/69‑1 (MQ=255) gAAACCGCACAGATGATGCTCGACGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGc > 1:2392748/1‑68 (MQ=255) gAAACCGCACAGATGATGCTCGACGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGc > 1:3012106/1‑68 (MQ=255) gAAACCGCACAGATGATGCTCGACGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGc > 1:1396947/1‑68 (MQ=255) gAAACCGCACAGATGATGCTCGACGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGc > 1:2906302/1‑68 (MQ=255) gAAACCGCACAGATGATGCTCGACGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGc > 1:167987/1‑68 (MQ=255) gAAACCGCACAGATGATGCTCGACGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGc > 1:1684350/1‑68 (MQ=255) gAAACAGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCt < 1:681798/69‑1 (MQ=255) cGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAg > 1:2142165/1‑68 (MQ=255) cGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAg > 1:3277861/1‑68 (MQ=255) atgatgCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGaaaaaa > 1:2661044/1‑67 (MQ=255) atgatgCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGaaaaaa > 1:2449889/1‑67 (MQ=255) atgCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGTAAAAAGCg < 1:364902/67‑1 (MQ=255) atgCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGc > 1:950939/1‑66 (MQ=255) atgCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCg < 1:1293680/67‑1 (MQ=255) atgCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCg > 1:2082651/1‑67 (MQ=255) atgCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCg > 1:1206606/1‑67 (MQ=255) atgCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCg < 1:243630/67‑1 (MQ=255) atgCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCg > 1:140514/1‑67 (MQ=255) atgCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCg > 1:1452431/1‑67 (MQ=255) atgCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCg < 1:220786/67‑1 (MQ=255) atgCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGa > 1:459576/1‑68 (MQ=255) atgCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGa > 1:353303/1‑68 (MQ=255) atgCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGa > 1:2285030/1‑68 (MQ=255) gCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGc < 1:317890/68‑1 (MQ=255) gCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGc < 1:1960740/68‑1 (MQ=255) cGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAAc > 1:2330143/1‑68 (MQ=255) cGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAAc > 1:1643984/1‑68 (MQ=255) cGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAAc > 1:1751039/1‑68 (MQ=255) cGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAAc > 1:1947190/1‑68 (MQ=255) tgatCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTgg < 1:3231040/68‑1 (MQ=255) tgatCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTgg < 1:3148373/68‑1 (MQ=255) tgatCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTgg < 1:900633/68‑1 (MQ=255) | CTGTTTTACCGACTGGCAACAGGTTCAGGAAGATATCGAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGG > minE/817375‑817502 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |