| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 79,533 | T→C | 54.9% | F48F (TTT→TTC) | murF → | UDP‑N‑acetylmuramoyl‑tripeptide:D‑alanyl‑D‑ alanine ligase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 79,533 | 0 | T | C | 54.9% | 2.2 / 21.6 | 31 | F48F (TTT→TTC) | murF | UDP‑N‑acetylmuramoyl‑tripeptide:D‑alanyl‑D‑ alanine ligase |
| Reads supporting (aligned to +/- strand): ref base T (8/6); new base C (2/15); total (10/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.81e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.62e-01 | |||||||||||
GCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTA > minE/79465‑79584 | gCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGtttt < 1:679330/69‑1 (MQ=255) cTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGtttt < 1:88475/68‑1 (MQ=255) cTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGtttt < 1:3136844/68‑1 (MQ=255) cTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGtttt < 1:346035/68‑1 (MQ=255) cTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGtttt < 1:202082/68‑1 (MQ=255) cTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGtttt < 1:204241/68‑1 (MQ=255) tGTAACCACTGACACGCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGa > 1:1121976/1‑69 (MQ=255) tGTAACCACTGACACGCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGa > 1:763409/1‑69 (MQ=255) gTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTTGAt > 1:562131/1‑69 (MQ=255) aaCTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGAcc > 1:1093593/1‑69 (MQ=255) aaCTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGAcc > 1:3130828/1‑69 (MQ=255) aaCTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGAcc > 1:2256065/1‑69 (MQ=255) aaCTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGAcc > 1:1813022/1‑69 (MQ=255) aaCTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGAcc > 1:708952/1‑69 (MQ=255) aaCTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGAcc > 1:971296/1‑69 (MQ=255) aaCTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGGTTTGATGCCCACGATTTTGCCGAcc > 1:3278991/1‑69 (MQ=255) aCTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCa < 1:692611/69‑1 (MQ=255) aCGCCGGGCTGGCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg < 1:3010220/58‑1 (MQ=255) aCGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATTATTTTg < 1:3131007/58‑1 (MQ=255) aCGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg < 1:561631/58‑1 (MQ=255) aCGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg < 1:1103409/58‑1 (MQ=255) aCGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg < 1:829489/58‑1 (MQ=255) aCGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg < 1:1570661/58‑1 (MQ=255) aCGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg < 1:1969102/58‑1 (MQ=255) aCGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg < 1:2003847/58‑1 (MQ=255) aCGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg < 1:3367727/58‑1 (MQ=255) aCGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg < 1:3302978/58‑1 (MQ=255) aCGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg < 1:2154420/58‑1 (MQ=255) aCGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg < 1:237902/58‑1 (MQ=255) aCGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg < 1:2475636/58‑1 (MQ=255) aCGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg < 1:3124411/58‑1 (MQ=255) cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg < 1:1887062/57‑1 (MQ=255) ccGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGtttt < 1:786266/39‑1 (MQ=255) cGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGtttt < 1:2907841/38‑1 (MQ=255) tGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCa < 1:2122265/56‑1 (MQ=255) cTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCacta < 1:677724/69‑1 (MQ=255) cTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCacta < 1:1443795/69‑1 (MQ=255) cTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCacta < 1:2596198/69‑1 (MQ=255) cTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGAAGGCGCacta < 1:1208579/69‑1 (MQ=255) | GCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTA > minE/79465‑79584 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |