| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 820,109 | G→A | 66.3% | M192I (ATG→ATA) | ychA → | predicted transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 820,109 | 0 | G | A | 66.3% | 50.2 / 38.0 | 71 | M192I (ATG→ATA) | ychA | predicted transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base G (4/20); new base A (28/19); total (32/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.63e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01 | |||||||||||
TAACATTGAGGTAATCCGCAAATTGCTGGATACACTCAAAGCCTCGTTGATGGAAGAAAATCAGATGGAGCTGGCGTTACGCACCAGCGAAGCTTTATTACAATTCAAC > minE/820058‑820166 | tAACATTGAGGTAATCCGCAAATTGCTGGATACACTCAAAGGTTCGTTGATAGAAGAAAATCAGATGGa > 1:3142492/1‑69 (MQ=255)tAACATTGAGGTAATCCGCAAATTGCTGGATACACTCAAAGCTTCGTTGATAGAAGTAAATCAGATGGa > 1:2996701/1‑69 (MQ=255)tAACATTGAGGTAATCCGCAAATTGCTGGATACACTCAAAGCTTCGTTGATAGAAGAAAATCAGATgg > 1:452125/1‑68 (MQ=255)tAACATTGAGGTAATCCGCAAATTGCTGGATACACTCAAAGCTTCGTTGATAGAAGAAAATCAGATgg > 1:1750673/1‑68 (MQ=255)tAACATTGAGGTAATCCGCAAATTGCTGGATACACTCAAAGCTTCGTTGATAGAAGAAAATCAGATGGa > 1:2676576/1‑69 (MQ=255)tAACATTGAGGTAATCCGCAAATTGCTGGATACACTCAAAGCTTCGTTGATAGAAGAAAATCAGATGGa > 1:3255566/1‑69 (MQ=255)tAACATTGAGGTAATCCGCAAATTGCTGGATACACTCAAAGCTTCGTTGATAGAAGAAAATCAGATGGa > 1:1944431/1‑69 (MQ=255)tAACATTGAGGTAATCCGCAAATTGCTGGATACACTCAAAGCTTCGTTGATAGAAGAAAATCAGATGGa > 1:195043/1‑69 (MQ=255)tAACATTGAGGTAATCCGCAAATTGCTGGATACACTCAAAGCTTCGTTGATAGAAGAAAATCAGATGGa > 1:199221/1‑69 (MQ=255)tAACATTGAGGTAATCCGCAAATTGCTGGATACACTCAAAGCTTCGTTGATAGAAGAAAATCAGATGGa > 1:2032344/1‑69 (MQ=255)tAACATTGAGGTAATCCGCAAATTGCTGGATACACTCAAAGCTTCGTTGATAGAAGAAAATCAGATGGa > 1:206552/1‑69 (MQ=255)tAACATTGAGGTAATCCGCAAATTGCTGGATACACTCAAAGCTTCGTTGATAGAAGAAAATCAGATGGa > 1:862200/1‑69 (MQ=255)tAACATTGAGGTAATCCGCAAATTGCTGGATACACTCAAAGCTTCGTTGATAGAAGAAAATCAGATGGa > 1:2187946/1‑69 (MQ=255)tAACATTGAGGTAATCCGCAAATTGCTGGATACACTCAAAGCTTCGTTGATAGAAGAAAATCAGATGGa > 1:3379040/1‑69 (MQ=255)tAACATTGAGGTAATCCGCAAATTGCTGGATACACTCAAAGCTTCGTTGATAGAAGAAAATCAGATGGa > 1:230726/1‑69 (MQ=255)tAACATTGAGGTAATCCGCAAATTGCTGGATACACTCAAAGCTTCGTTGATAGAAGAAAATCAGATGGa > 1:102710/1‑69 (MQ=255)tAACATTGAGGTAATCCGCAAATTGCTGGATACACTCAAAGCTTCGTTGATAGAAGAAAATCAGATGGa > 1:2364501/1‑69 (MQ=255)tAACATTGAGGTAATCCGCAAATTGCTGGATACACTCAAAGCTTCGTTGATAGAAGAAAATCAGATGGa > 1:2394744/1‑69 (MQ=255)tAACATTGAGGTAATCCGCAAATTGCTGGATACACTCAAAGCTTCGTTGATAGAAGAAAATCAGATGGa > 1:1841854/1‑69 (MQ=255)tAACATTGAGGTAATCCGCAAATTGCTGGATACACTCAAAGCTTCGTTGATAGAAGAAAATCAGATGGa > 1:2770988/1‑69 (MQ=255)tAACATTGAGGTAATCCGCAAATTGCTGGATACACTCAAAGCTTCGTTGATAGAAGAAAATCAGATGGa > 1:2814691/1‑69 (MQ=255)tAACATTGAGGTAATCCGCAAATTGCTGGATACACTCAAAGCTTCGTTGATAGAAGAAAATCAGATGGa > 1:577227/1‑69 (MQ=255)tAACATTGAGGTAATCCGCAAATTGCTGGATACACTCAAAGCTTCGTTGATAGAAGAAAATCAGATGGa > 1:1682604/1‑69 (MQ=255)tAACATTGAGGTAATCCGCAAATTGCTGGATACACTCAAAGCTTCGTTGATAGAAGAAAATCAGATGGa > 1:323228/1‑69 (MQ=255)tAACATTGAGGTAATCCGCAAATTGCTGGATACACTCAAAGCTTCGTTGATAGAAGAAAATCAGATGGa > 1:795963/1‑69 (MQ=255)tAACATTGAGGTAATCCGCAAATTGCTGGATACACTCAAAGCTTCGTTGATAGAAGAAAATCAGATGGa > 1:12256/1‑69 (MQ=255)tAACATTGAGGTAATCCGCAAATTGCTGGATACACTCAAAGCTTCGTTGATAGAAGAAAATCAGATGGa > 1:302621/1‑69 (MQ=255)tAACATTGAGGTAATCCGCAAATTGCTGGATACACTCAAAGCTTAGTTGATAGAAGAAAATCAGATGGa > 1:482524/1‑69 (MQ=255) ggTAATCCGCAAATTGCTGGATACACTCAAAGCCTCGTTGATGGAAGAAAATCAGATGGAGCTGGCGtt > 1:3285550/1‑69 (MQ=255) ggTAATCCGCAAATTGCTGGATACACTCAAAGCCTCGTTGATGGAAGAAAATCAGATGGAGCTGGCGtt > 1:1565761/1‑69 (MQ=255) ggTAATCCGCAAATTGCTGGATACACTCAAAGCCTCGTTGATGGAAGAAAATCAGATGGAGCTGGCGtt > 1:1173825/1‑69 (MQ=255) ggTAATCCGCAAATTGCTGGATACACTCAAAGCCTCGTTGATGGAAGAAAATCAGATGGAGCTGGCGtt > 1:2589083/1‑69 (MQ=255) aTTGCTGGATACACTCAAAGCCTCGTTGATGGAAGAAAATCAGATGGAGCTGGCGTTACGCACCAGCGa < 1:2838062/69‑1 (MQ=255) aTTGCTGGATACACTCAAAGCCTCGTTGATGGAAGAAAATCAGATGGAGCTGGCGTTACGCACCAGCGa < 1:3127987/69‑1 (MQ=255) aTTGCTGGATACACTCAAAGCCTCGTTGATGGAAGAAAATCAGATGGAGCTGGCGTTACGCACCAGCGa < 1:836538/69‑1 (MQ=255) aTTGCTGGATACACTCAAAGCCTCGTTGATGGAAGAAAATCAGATGGAGCTGGCGTTACGCACCAGCGa < 1:2168116/69‑1 (MQ=255) aTTGCTGGATACACTCAAAGCCTCGTTGATGGAAGAAAATCAGATGGAGCTGGCGTTACGCACCAGCGa < 1:476679/69‑1 (MQ=255) ggATACACTCAAAGCCTCGTTGATGGAAGAAAATCAGATGGAGCTGGCGTTACGCACCAGCGAAGCtt < 1:3375240/68‑1 (MQ=255) ggATACACTCAAAGCCTCGTTGATGGAAGAAAATCAGATGGAGCTGGCGTTACGCACCAGCGAAGCtt < 1:3074229/68‑1 (MQ=255) ggATACACTCAAAGCCTCGTTGATGGAAGAAAATCAGATGGAGCTGGCGTTACGCACCAGCGAAGCtt < 1:1146641/68‑1 (MQ=255) ggATACACTCAAAGCCTCGTTGATGGAAGAAAATCAGATGGAGCTGGCGTTACGCACCAGCGAAGCtt < 1:1911357/68‑1 (MQ=255) ggATACACTCAAAGCCTCGTTGATGGAAGAAAATCAGATGGAGCTGGCGTTACGCACCAGCGAAGCtt < 1:1780894/68‑1 (MQ=255) ggATACACTCAAAGCCTCGTTGATGGAAGAAAATCAGATGGAGCTGGCGTTACGCACCAGCGAAGCtt < 1:577813/68‑1 (MQ=255) tACACTCAAAGCTTCGTTGATAGAAGAAAATCAGATGGAGCTGGCGTTACGCACCAGCGAAGCTttat < 1:2324733/68‑1 (MQ=255) tACACTCAAAGCTTCGTTGATAGAAGAAAATCAGATGGAGCTGGCGTTACGCACCAGCGAAGCTttat < 1:79652/68‑1 (MQ=255) tACACTCAAAGCTTCGTTGATAGAAGAAAATCAGATGGAGCTGGCGTTACGCACCAGCGAAGCTttat < 1:2889277/68‑1 (MQ=255) tACACTCAAAGCTTCGTTGATAGAAGAAAATCAGATGGAGCTGGCGTTACGCACCAGCGAAGCTttat < 1:1635508/68‑1 (MQ=255) tACACTCAAAGCTTCGTTGATAGAAGAAAATCAGATGGAGCTGGCGTTACGCACCAGCGAAGCTttat < 1:366349/68‑1 (MQ=255) aGCTTCGTTGATAGAAGAAAATCAGATGGAGCTGGCGTTACGCACCAGCGAAGCTTTATTACAATTc < 1:3290489/67‑1 (MQ=255) aGCTTCGTTGATAGAAGAAAATCAGATGGAGCTGGCGTTACGCACCAGCGAAGCTTTATTACAATTc < 1:3331161/67‑1 (MQ=255) aGCTTCGTTGATAGAAGAAAATCAGATGGAGCTGGCGTTACGCACCAGCGAAGCTTTATTACAATTc < 1:2617990/67‑1 (MQ=255) aGCTTCGTTGATAGAAGAAAATCAGATGGAGCTGGCGTTACGCACCAGCGAAGCTTTATTACAATTc < 1:1119139/67‑1 (MQ=255) aGCTTCGTTGATAGAAGAAAATCAGATGGAGCTGGCGTTACGCACCAGCGAAGCTTTATTACAATTc < 1:2836885/67‑1 (MQ=255) aGCTTCGTTGATAGAAGAAAATCAGATGGAGCTGGCGTTACGCACCAGCGAAGCTTTATTACAATTc < 1:2697050/67‑1 (MQ=255) aGCTTCGTTGATAGAAGAAAATCAGATGGAGCTGGCGTTACGCACCAGCGAAGCTTTATTACAATTc < 1:2634684/67‑1 (MQ=255) aGCTTCGTTGATAGAAGAAAATCAGATGGAGCTGGCGTTACGCACCAGCGAAGCTTTATTACAATTc < 1:2621205/67‑1 (MQ=255) aGCTTCGTTGATAGAAGAAAATCAGATGGAGCTGGCGTTACGCACCAGCGAAGCTTTATTACAATTc < 1:1538599/67‑1 (MQ=255) aGCTTCGTTGATAGAAGAAAATCAGATGGAGCTGGCGTTACGCACCAGCGAAGCTTTATTACAATTc < 1:2201644/67‑1 (MQ=255) aGCTTCGTTGATAGAAGAAAATCAGATGGAGCTGGCGTTACGCACCAGCGAAGCTTTATTACAATTc < 1:1671353/67‑1 (MQ=255) aGCTTCGTTGATAGAAGAAAATCAGATGGAGCTGGCGTTACGCACCAGCGAAGCTTTATTACAATTc < 1:1736004/67‑1 (MQ=255) aGCTTCGTTGATAGAAGAAAATCAGATGGAGCTGGCGTTACGCACCAGCGAAGCTTTATTACAATTc < 1:1756516/67‑1 (MQ=255) gCCTCGTTGATGGAAGAAAATCAGATGGAGCTGGCGTTACGCACCAGCGAAGCTTTATTACAATTCAAc < 1:2976890/69‑1 (MQ=255) gCCTCGTTGATGGAAGAAAATCAGATGGAGCTGGCGTTACGCACCAGCGAAGCTTTATTACAATTCAAc < 1:1187624/69‑1 (MQ=255) gCCTCGTTGATGGAAGAAAATCAGATGGAGCTGGCGTTACGCACCAGCGAAGCTTTATTACAATTCAAc < 1:542390/69‑1 (MQ=255) gCCTCGTTGATGGAAGAAAATCAGATGGAGCTGGCGTTACGCACCAGCGAAGCTTTATTACAATTCAAc < 1:1750337/69‑1 (MQ=255) gCCTCGTTGATGGAAGAAAATCAGATGGAGCTGGCGTTACGCACCAGCGAAGCTTTATTACAATTCAAc < 1:184906/69‑1 (MQ=255) gCCTCGTTGATGGAAGAAAATCAGATGGAGCTGGCGTTACGCACCAGCGAAGCTTTATTACAATTCAAc < 1:2096251/69‑1 (MQ=255) gCCTCGTTGATGGAAGAAAATCAGATGGAGCTGGCGTTACGCACCAGCGAAGCTTTATTACAATTCAAc < 1:247604/69‑1 (MQ=255) gCCTCGTTGATGGAAGAAAATCAGATGGAGCTGGCGTTACGCACCAGCGAAGCTTTATTACAATTCAAc < 1:2668380/69‑1 (MQ=255) gCCTCGTTGATGGAAGAAAATCAGATGGAGCTGGCGTTACGCACCAGCGAAGCTTTATTACAATTCAAc < 1:3117468/69‑1 (MQ=255) gATAGAAGAAAATCAGATGGAGCTGGCGTTACGCACCAGCGAAGCTTTATTACAATTc < 1:107058/58‑1 (MQ=255) | TAACATTGAGGTAATCCGCAAATTGCTGGATACACTCAAAGCCTCGTTGATGGAAGAAAATCAGATGGAGCTGGCGTTACGCACCAGCGAAGCTTTATTACAATTCAAC > minE/820058‑820166 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |