| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 821,005 | G→A | 39.5% | P209P (CCG→CCA) | kdsA → | 3‑deoxy‑D‑manno‑octulosonate 8‑phosphate synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 821,005 | 0 | G | A | 39.5% | 24.1 / 33.0 | 53 | P209P (CCG→CCA) | kdsA | 3‑deoxy‑D‑manno‑octulosonate 8‑phosphate synthase |
| Reads supporting (aligned to +/- strand): ref base G (2/30); new base A (18/3); total (20/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.29e-09 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.00e-01 | |||||||||||
TGGTAACTCGCCGGTGATTTTCGACGTGACCCACGCACTGCAATGCCGCGATCCGTTTGGCGCAGCTTCCGGTGGTCGTCGTGCTCAGGTGGCTGAGCTGGCACGAGCCGGTATGGC > minE/820951‑821067 | tGGTAACTCGCCGGTGATTTTCGACGTGACCCACGCACTGCAATGCCGCGATCCGTTTGGCGCAGCTTc < 1:1285074/69‑1 (MQ=255)tGGTAACTCGCCGGTGATTTTCGACGTGACCCACGCACTGCAATGCCGCGATCCGTTTGGCGCAGCTTc < 1:2285397/69‑1 (MQ=255) tttCGACGTGACCCACGCACTGCAATGCCGCGATCCGTTTGGCGCAGCTTCCGGTGGTCGTCGTGCTCa < 1:2967138/69‑1 (MQ=255) tttCGACGTGACCCACGCACTGCAATGCCGCGATCCGTTTGGCGCAGCTTCCGGTGGTCGTCGTGCTCa < 1:1772516/69‑1 (MQ=255) tttCGACGTGACCCACGCACTGCAATCCCGCGATCCGTTTGGCGCAGCTTCCGGTGGTCGTCGTGCTCa < 1:2231137/69‑1 (MQ=255) ttCGACGTGACCCACGCACTGCAATGCCGCGATCCGTTTGGCGCAGCTTCCGGTGGTCGTCGTGCTCa < 1:1003026/68‑1 (MQ=255) tGACCCACGCACTGCAATGCCGCGATCCGTTTGGCGCAGCTTCCGGTGGTCGTCGTGCTCAGGTGGCTg < 1:3169277/69‑1 (MQ=255) tGACCCACGCACTGCAATGCCGCGATCCGTTTGGCGCAGCTTCCGGTGGTCGGCGTGCTCAGGTGGCTg < 1:1674045/69‑1 (MQ=255) aCCCACGCACTGCAATGTCGCGATCCATTTGGCGCAGCTTcc > 1:1836794/1‑42 (MQ=37) aCCCACGCACTGCAATGTCGCGATCCATTTGGCGCAGCTTcc > 1:2372961/1‑42 (MQ=37) aCCCACGCACTGCAATGTCGCGATCCATTTGGCGCAGCTTcc > 1:2688820/1‑42 (MQ=37) aCCCACGCACTGCAATGTCGCGATCCATTTGGCGCAGCTTcc > 1:2248500/1‑42 (MQ=37) aCCCACGCACTGCAATGTCGCGATCCATTTGGCGCAGCTTcc > 1:2223960/1‑42 (MQ=37) aCCCACGCACTGCAATGTCGCGATCCATTTGGCGCAGCTTcc > 1:2159067/1‑42 (MQ=37) aCCCACGCACTGCAATGTCGCGATCCATTTGGCGCAGCTTcc > 1:3248353/1‑42 (MQ=37) aCCCACGCACTGCAATGTCGCGATCCATTTGGCGCAGCTTcc > 1:1845407/1‑42 (MQ=37) aCCCACGCACTGCAATGTCGCGATCCATTTGGCGCAGCTTcc > 1:3397273/1‑42 (MQ=37) aCCCACGCACTGCAATGTCGCGATCCATTTGGCGCAGCTTcc > 1:1810549/1‑42 (MQ=37) aCCCACGCACTGCAATGTCGCGATCCATTTGGCGCAGCTTcc > 1:1808122/1‑42 (MQ=37) aCCCACGCACTGCAATGTCGCGATCCATTTGGCGCAGCTTcc > 1:539671/1‑42 (MQ=37) aCCCACGCACTGCAATGTCGCGATCCATTTGGCGCAGCTTcc > 1:613002/1‑42 (MQ=37) aCCCACGCACTGCAATGTCGCGATCCATTTGGCGCAGCTTcc > 1:1655583/1‑42 (MQ=37) aCCCACGCACTGCAATGTCGCGATCCATTTGGCGCAGCTTcc > 1:1412011/1‑42 (MQ=37) aCCCACGCACTGCAATGTCGCGATCCATTTGGCGCAGCTTcc > 1:743525/1‑42 (MQ=37) aCCCACGCACTGCAATGTCGCGATCCATTTGGCGCAGCTTcc > 1:1559729/1‑42 (MQ=37) aCCCACGCACTGCAATGTCGCGATCCATTTGGCGCAGCTTc > 1:3216017/1‑41 (MQ=37) aCCCACGCACTGCAATGCCGCGATCCGTTTGGCGCAGCTTCCGGTGGTCGTCGTGCTCAGGTGGCTGAg > 1:2334443/1‑69 (MQ=255) aCCCACGCACTGCAATGCCGAGATCCGTTTGGCGCAGCTTCCGGTGGTCGTCGTGCTCAGGTGGCTGAg > 1:108339/1‑69 (MQ=255) cACGCACTGCAATGCCGCGATCCGTTTGGCGCAGCTTCCGGTGGTCGGCGTGCTCAGGTGGCTg < 1:296607/64‑1 (MQ=255) aTGTCGCGATCCATTTGGCGCAGCTTCCGGCGGTCGCCGTGCTCAGGTGGCTGAGCTGGCAAGAGcc < 1:2804642/67‑1 (MQ=255) aTGTCGCGATCCATTTGGCGCAGCTTCCGGCGGTCGCCGTGCTCAGGTGGCTGAGCTGGCAAGAGcc < 1:1632833/67‑1 (MQ=255) aTGTCGCGATCCATTTGGCGCAGCTTCCGGCGGTCGCCGTGCTCAGGTGGCTGAGCTGGCAAGAGcc < 1:1843741/67‑1 (MQ=255) aTGCCGCGATCCGTTTGGCTCAGCTTCCGGTGGTCGTCGTGCTCAGGTGGCTGAGCTGGCACGAGCCgg < 1:912482/69‑1 (MQ=255) aTGCCGCGATCCGTTTGGCGCAGCTTCCGGTGGTCGTCGTGCTCAGGTGGCTGAGCTGGCACGAGCCgg < 1:2006786/69‑1 (MQ=255) aTGCCGCGATCCGTTTGGCGCAGCTTCCGGTGGTCGTCGTGCTCAGGTGGCTGAGCTGGCACGAGCCgg < 1:1263678/69‑1 (MQ=255) aTGCCGCGATCCGTTTGGCGCAGCTTCCGGTGGTCGTCGTGCTCAGGTGGCTGAGCTGGCACGAGCCgg < 1:1610949/69‑1 (MQ=255) aTGCCGCGATCCGTTTGGCGCAGCTTCCGGTGGTCGTCGTGCTCAGGTGGCTGAGCTGGCACGAGCCgg < 1:1685421/69‑1 (MQ=255) aTGCCGCGATCCGTTTGGCGCAGCTTCCGGTGGTCGTCGTGCTCAGGTGGCTGAGCTGGCACGAGCCgg < 1:611717/69‑1 (MQ=255) aTGCCGCGATCCGTTTGGCGCAGCTTCCGGTGGTCGTCGTGCTCAGGTGGCTGAGCTGGCACGAGCCgg < 1:1752878/69‑1 (MQ=255) aTGCCGCGATCCGTTTGGCGCAGCTTCCGGTGGTCGTCGTGCTCAGGTGGCTGAGCTGGCACGAGCCgg < 1:390994/69‑1 (MQ=255) aTGCCGCGATCCGTTTGGCGCAGCTTCCGGTGGTCGTCGTGCTCAGGTGGCTGAGCTGGCACGAGCCgg < 1:1834614/69‑1 (MQ=255) aTGCCGCGATCCGTTTGGCGCAGCTTCCGGTGGTCGTCGTGCTCAGGTGGCTGAGCTGGCACGAGCCgg < 1:2519931/69‑1 (MQ=255) aTGCCGCGATCCGTTTGGCGCAGCTTCCGGTGGTCGTCGTGCTCAGGTGGCTGAGCTGGCACGAGCCgg < 1:2250642/69‑1 (MQ=255) aTGCCGCGATCCGTTTGGCGCAGCTTCCGGTGGTCGTCGTGCTCAGGTGGCTGAGCTGGCACGAGCCgg < 1:2268090/69‑1 (MQ=255) aTGCCGCGATCCGTTTGGCGCAGCTTCCGGTGGTCGTCGTGCTCAGGTGGCTGAGCTGGCACGAGCCgg < 1:2269991/69‑1 (MQ=255) aTGCCGCGATCCGTTTGGCGCAGCTTCCGGTGGTCGTCGTGCTCAGGTGGCTGAGCTGGCACGAGCCgg < 1:2941368/69‑1 (MQ=255) aTGCCGCGATCCGTTTGGCGCAGCTTCCGGTGGTCGTCGTGCTCAGGTGGCTGAGCTGGCACGAGCCgg < 1:2937427/69‑1 (MQ=255) aTGCCGCGATCCGTTTGGCGCAGCTTCCGGTGGTCGTCGTGCTCAGGTGGCTGAGCTGGCACGAGCCgg < 1:2301656/69‑1 (MQ=255) aTGCCGCGATCCGTTTGGCGCAGCTTCCGGTGGTCGTCGTGCTCAGGTGGCTGAGCTGGCACGAGCCgg < 1:2305849/69‑1 (MQ=255) aTGCCGCGATCCGTTTGGCGCAGCTTCCGGTGGTCGTCGTGCTCAGGGGGCTGAGCTGGCACGAGCCgg < 1:2945171/69‑1 (MQ=255) tGCCGCGATCCGTTTGGCGCAGCTTCCGGTGGTCGTCGTGCTCAGGTGGCTGAGCTGGCACGAGCCgg < 1:781686/68‑1 (MQ=255) cgATCCGTTTGGCGCAGCTTCCGGTGGTCGTCGTGCTCAGGTGGCTGAGCTGGCACGAGCCGGTATGGc < 1:1886486/69‑1 (MQ=255) cgATCCGTTTGGCGCAGCTTCCGCTGGTCGTCGTGCTCAGGTGGCTGAGCTGGCACGAGCCGGTATGGc < 1:264384/69‑1 (MQ=255) | TGGTAACTCGCCGGTGATTTTCGACGTGACCCACGCACTGCAATGCCGCGATCCGTTTGGCGCAGCTTCCGGTGGTCGTCGTGCTCAGGTGGCTGAGCTGGCACGAGCCGGTATGGC > minE/820951‑821067 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |