| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 824,680 | T→C | 35.9% | D172D (GAT→GAC) | chaC → | regulatory protein for cation transport |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 824,680 | 0 | T | C | 35.9% | 37.9 / 37.1 | 64 | D172D (GAT→GAC) | chaC | regulatory protein for cation transport |
| Reads supporting (aligned to +/- strand): ref base T (28/13); new base C (4/19); total (32/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.86e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.32e-01 | |||||||||||
ATGGACGCACAGTAAACGCCATTGTGTTTATTATGGACCCGCGACATCCAGAATATGAATCTGATACTCGCGCTCAGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCAC > minE/824616‑824744 | atgGACGCACAGTAAACGCCATTGTGTTTATTATGGACCCGCGACATCCAGAATATGAATCTGATACTc > 1:1138881/1‑69 (MQ=255)atgGACGCACAGTAAACGCCATTGTGTTTATTATGGACCCGCGACATCCAGAATATGAATCTGATACTc > 1:2541956/1‑69 (MQ=255)atgGACGCACAGTAAACGCCATTGTGTTTATTATGGACCCGCGACATCCAGAATATGAATCTGATACTc > 1:330061/1‑69 (MQ=255)atgGACGCACAGTAAACGCCATTGTGTTTATTATGGACCCGCGACATCCAGAATATGAATCTGATACTc > 1:3183669/1‑69 (MQ=255)atgGACGCACAGTAAACGCCATTGTGTTTATTATGGACCCGCGACATCCAGAATATGAATCTGATACTc > 1:1902199/1‑69 (MQ=255) gTAAACGCCATTGTGTTTATTATGGACCCGCGACATCCAGAATATGAATCTGACACTCGCGCTCAGGt > 1:888436/1‑68 (MQ=255) gTAAACGCCATTGTGTTTATTATGGACCCGCGACATCCAGAATATGAATCTGACACTCGCGCTCAGGt > 1:2061953/1‑68 (MQ=255) gTAAACGCCATTGTGTTTATTATGGACCCGCGACATCCAGAATATGAATCTGACACTCGCGCTCAGGt > 1:2588018/1‑68 (MQ=255) gTAAACGCCATTGTGTTTATTATGGACCCGCGACATCCAGAATATGAATCTGACACTCGCGCTCAGGt > 1:3072837/1‑68 (MQ=255) aaCGCCATTGTGTTTATTATGGACCCGCGACATCCAGAATATGAATCTGATACTCGCGCTCAGGTCAt > 1:1675614/1‑68 (MQ=255) aaCGCCATTGTGTTTATTATGGACCCGCGACATCCAGAATATGAATCTGATACTCGCGCTCAGGTCAt > 1:1537371/1‑68 (MQ=255) aaCGCCATTGTGTTTATTATGGACCCGCGACATCCAGAATATGAATCTGATACTCGCGCTCAGGTCATc > 1:3145946/1‑69 (MQ=255) aaCGCCATTGTGTTTATTATGGACCCGCGACATCCAGAATATGAATCTGATACTCGCGCTCAGGTCATc > 1:975251/1‑69 (MQ=255) aaCGCCATTGTGTTTATTATGGACCCGCGACATCCAGAATATGAATCTGATACTCGCGCTCAGGTCATc > 1:280221/1‑69 (MQ=255) aaCGCCATTGTGTTTATTATGGACCCGCGACATCCAGAATATGAATCTGATACTCGCGCTCAGGTCATc > 1:3149769/1‑69 (MQ=255) aaCGCCATTGTGTTTATTATGGACCCGCGACATCCAGAATATGAATCTGATACTCGCGCTCAGGTCATc > 1:3373784/1‑69 (MQ=255) aaCGCCATTGTGTTTATTATGGACCCGCGACATCCAGAATATGAATCTGATACTCGCGCTCAGGTCATc > 1:874849/1‑69 (MQ=255) aaCGCCATTGTGTTTATTATGGACCCGCGACATCCAGAATATGAATCTGATACTCGCGCTCAGGTCATc > 1:216239/1‑69 (MQ=255) aaCGCCATTGTGTTTATTATGGACCCGCGACATCCAGAATATGAATCTGATACTCGCGCTCAGGTCATc > 1:1249547/1‑69 (MQ=255) aaCGCCATTGTGTTTATTATGGACCCGCGACATCCAGAATATGAATCTGATACTCGCGCTCAGGTCATc > 1:1251357/1‑69 (MQ=255) aaCGCCATTGTGTTTATTATGGACCCGCGACATCCAGAATATGAATCTGATACTCGCGCTCAGGTCATc > 1:1521826/1‑69 (MQ=255) tgtgTTTATTATGGACCCGCGACATCCAGAATATGAATCTGATACTCGCGCTCAGGTCATCgcgc > 1:2459037/1‑65 (MQ=255) tgtgTTTATTATGGACCCGCGACATCCAGAATATGAATCTGATACTCGCGCTCAGGTCATCGCGcc > 1:3237487/1‑66 (MQ=255) tgtgTTTATTATGGACCCGCGACATCCAGAATATGAATCTGATACTCGCGCTCAGGTCATCGCGCCGtt > 1:9491/1‑69 (MQ=255) tgtgTTTATTATGGACCCGCGACATCCAGAATATGAATCTGATACTCGCGCTCAGGTCATCGCGCCGtt > 1:2346830/1‑69 (MQ=255) tgtgTTTATTATGGACCCGCGACATCCAGAATATGAATCTGATACTCGCGCTCAGGTCATCGCGCCGtt > 1:3187329/1‑69 (MQ=255) tgtgTTTATTATGGACCCGCGACATCCAGAATATGAATCTGATACTCGCGCTCAGGTCATCGCGCCGtt > 1:1367911/1‑69 (MQ=255) tgtgTTTATTATGGACCCGCGACATCCAGAATATGAATCTGATACTCGCGCTCAGGTCATCGCGCCGtt > 1:2904044/1‑69 (MQ=255) tgtgTTTATTATGGACCCGCGACATCCAGAATATGAATCTGATACTCGCGCTCAGGTCATCGCGCCGtt > 1:2873567/1‑69 (MQ=255) tgtgTTTATTATGGACCCGCGACATCCAGAATATGAATCTGATACTCGCGCTCAGGTCATCGCGCCGt > 1:3378950/1‑68 (MQ=255) gtgtTTATTATGGACCCGCGACATCCAGAATATGAATCTGATACTCGCGCTCAGGTCATCGCGCCGTTg < 1:210613/69‑1 (MQ=255) gtgtTTATTATGGACCCGCGACATCCAGAATATGAATCTGATACTCGCGCTCAGGTCATCGCGCCGTTg < 1:2005435/69‑1 (MQ=255) cccGCGACATCCAGAATATGAATCTGACACTCGCGCTTAGGTCATAGCGCCGTTGATTGCGGCGGCGAg < 1:257282/69‑1 (MQ=255) cccGCGACATCCAGAATATGAATCTGACACTCGCGCTCAGGTCATAGCGCCGTTGATTGCGGCGGCGAg < 1:2466179/69‑1 (MQ=255) cccGCGACATCCAGAATATGAATCTGACACTCGCGCTCAGGTCATAGCGCCGTTGATTGCGGCGGCGAg < 1:1001801/69‑1 (MQ=255) cccGCGACATCCAGAATATGAATCTGACACTCGCGCTCAGGTCATAGCGCCGTTGATTGCGGCGGCGAg < 1:2121950/69‑1 (MQ=255) cccGCGACATCCAGAATATGAATCTGACACTCGCGCTCAGGTCATAGCGCCGTTGATTGCGGCGGCGAg < 1:924288/69‑1 (MQ=255) cccGCGACATCCAGAATATGAATCTGACACTCGCGCTCAGGTCATAGCGCCGTTGATTGCGGCGGCGAg < 1:2222011/69‑1 (MQ=255) cccGCGACATCCAGAATATGAATCTGACACTCGCGCTCAGGTCATAGCGCCGTTGATTGCGGCGGCGAg < 1:769747/69‑1 (MQ=255) cccGCGACATCCAGAATATGAATCTGACACTCGCGCTCAGGTCATAGCGCCGTTGATTGCGGCGGCGAg < 1:751745/69‑1 (MQ=255) cccGCGACATCCAGAATATGAATCTGACACTCGCGCTCAGGTCATAGCGCCGTTGATTGCGGCGGCGAg < 1:670634/69‑1 (MQ=255) cccGCGACATCCAGAATATGAATCTGACACTCGCGCTCAGGTCATAGCGCCGTTGATTGCGGCGGCGAg < 1:604066/69‑1 (MQ=255) cccGCGACATCCAGAATATGAATCTGACACTCGCGCTCAGGTCATAGCGCCGTTGATTGCGGCGGCGAg < 1:43017/69‑1 (MQ=255) cccGCGACATCCAGAATATGAATCTGACACTCGCGCTCAGGTCATAGCGCCGTTGATTGCGGCGGCGAg < 1:3394129/69‑1 (MQ=255) cccGCGACATCCAGAATATGAATCTGACACTCGCGCTCAGGTCATAGCGCCGTTGATTGCGGCGGCGAg < 1:1253500/69‑1 (MQ=255) cccGCGACATCCAGAATATGAATCTGACACTCGCGCTCAGGTCATAGCGCCGTTGATTGCGGCGGCGAg < 1:327010/69‑1 (MQ=255) cccGCGACATCCAGAATATGAATCTGACACTCGCGCTCAGGTCATAGCGCCGTTGATTGCGGCGGCGAg < 1:2780881/69‑1 (MQ=255) cccGCGACATCCAGAATATGAATCTGACACTCGCGCTCAGGTCATAGCGCCGTTGATTGCGGCGGCGAg < 1:1902027/69‑1 (MQ=255) cccGCGACATCCAGAATATGAATCTGACACTCGCGCTCAGGTCATAGCGCCGTTGATTGCGGCGGCGAg < 1:2905748/69‑1 (MQ=255) cccGCGACATCCAGAATATGAATCTGACACTCGCGCTCAGGTCATAGCGCCGTTGATTGCGGCGGCGAg < 1:2492591/69‑1 (MQ=255) cccGCGACATCCAGAATATGAATCTGACACTCGCGCTCAGGTCATAGCGCCGTTGATTGCGGCGGCGAg < 1:1275311/69‑1 (MQ=255) aCATCCAGAATATGAATCTGATACTCGCGCTCAGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTcc < 1:252658/69‑1 (MQ=255) aCATCCAGAATATGAATCTGATACTCGCGCTCAGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTcc < 1:703908/69‑1 (MQ=255) aCATCCAGAATATGAATCTGATACTCGCGCTCAGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTcc < 1:867596/69‑1 (MQ=255) aCATCCAGAATATGAATCTGATACTCGCGCTCAGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTcc < 1:2714164/69‑1 (MQ=255) cATCCAGAATATGAATCTGATACTCGCGCTCAGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTcc < 1:1679424/68‑1 (MQ=255) cATCCAGAATATGAATCTGATACTCGCGCTCAGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTcc < 1:902837/68‑1 (MQ=255) cATCCAGAATATGAATCTGATACTCGCGCTCAGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTcc < 1:2597721/68‑1 (MQ=255) cATCCAGAATATGAATCTGATACTCGCGCTCAGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTCCg < 1:1711553/69‑1 (MQ=255) cATCCAGAATATGAATCTGATACTCGCGCTCAGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTCCg < 1:3001366/69‑1 (MQ=255) cAGAATATGAATCTGATACTCGCGCTCAGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTCCGCTg > 1:2456573/1‑68 (MQ=255) cAGAATATGAATCTGATACTCGCGCTCAGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTCCGCTg > 1:963883/1‑68 (MQ=255) tatGAATCTGATACTCGCGCTCAGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTCCg < 1:46929/60‑1 (MQ=255) cTGATACTCGCGCTCAGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGcac < 1:1925011/69‑1 (MQ=255) | ATGGACGCACAGTAAACGCCATTGTGTTTATTATGGACCCGCGACATCCAGAATATGAATCTGATACTCGCGCTCAGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCAC > minE/824616‑824744 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |