Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	826,676	C→A	53.6%	A358E (GCG→GAG)	ychP →	predicted invasin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	826,676	0	C	A	53.6%	‑2.3 / 45.4	41	A358E (GCG→GAG)	ychP	predicted invasin
Reads supporting (aligned to +/- strand):  ref base C (9/10);  new base A (2/20);  total (11/30)
Fisher's exact test for biased strand distribution p-value = 1.15e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GATTTGGCAGGGCGATACGCAGATATTAAGTTTGACGCCGGGCGCACAAGCCAACAGCGCGGAGGGCTGGACGCTGATCATGCCTGACTGGCAGAACGGGGAAGGGGCAAGCAATCACTGGCG  >  minE/826617‑826739                                                            |                                                                gATTTGGCAGGGCGATACGCAGATATTAAGTTTGACGCCGGGCGCACAAGCCAACAGCGCGGAGGGCTg                                                        >  1:3427943/1‑69 (MQ=255)     tGGCAGGGCGATACGCAGATATTAAGTTTGACGCCGGGCGCACAAGCCAACAGCGCGGAGGGCTGGACg                                                    >  1:2888866/1‑69 (MQ=255)     tGGCAGGGCGATACGCAGATATTAAGTTTGACGCCGGGCGCACAAGCCAACAGCGCGGAGGGCTGGACg                                                    >  1:2723362/1‑69 (MQ=255)     tGGCAGGGCGATACGCAGATATTAAGTTTGACGCCGGGCGCACAAGCCAACAGCGCGGAGGGCTGGACg                                                    >  1:2632073/1‑69 (MQ=255)     tGGCAGGGCGATACGCAGATATTAAGTTTGACGCCGGGCGCACAAGCCAACAGCGCGGAGGGCTGGACg                                                    >  1:2529747/1‑69 (MQ=255)     tGGCAGGGCGATACGCAGATATTAAGTTTGACGCCGGGCGCACAAGCCAACAGCGCGGAGGGCTGGACg                                                    >  1:498993/1‑69 (MQ=255)     tGGCAGGGCGATACGCAGATATTAAGTTTGACGCCGGGCGCACAAGCCAACAGCGCGGAGGGCTGGACg                                                    >  1:3295120/1‑69 (MQ=255)     tGGCAGGGCGATACGCAGATATTAAGTTTGACGCCGGGCGCACAAGCCAACAGCGCGGAGGGCTGGACg                                                    >  1:2179344/1‑69 (MQ=255)               aTACGCAGATATTAAGTTTGACGCCGGGCGCACAAGCCAACAGCGCGGAGGGCTGGACGCTGATCATGc                                          <  1:2908444/69‑1 (MQ=255)               aTACGCAGATATTAAGTTTGACGCCGGGCGCACAAGCCAACAGCGCGGAGGGCTGGACGCTGATCATGc                                          <  1:1599626/69‑1 (MQ=255)                tACGCAGATATTAAGTTTGACGCCGGGCGCACAAGCCAACAGCGCGGAGGGCTGGACGCTGATCATGc                                          <  1:2516029/68‑1 (MQ=255)                 aCGCAGATATTAAGTTTGACGCCGGGCGCACAAGCCAACAGCGCGGAGGGCTGGACGCTGATCATGCCt                                        <  1:2421512/69‑1 (MQ=255)                         atTAAGTTTGACGCCGGGCGCACAAGCCAACAGCGCGGAGGGCTGGACGCTGATCATGCCTGACTgg                                  >  1:1511433/1‑67 (MQ=255)                                 tGACGCCGGGCGCACAAGCCAACAGTGAGGAGGGCTGGACGCTGATCATGCCTGACTGGCAAAACgg                          >  1:382767/1‑67 (MQ=255)                                 tGACGCCGGGCGCACAAGCCAACAGTGAGGAGGGCTGGACGCTGATCATGCCTGACTGGCAAAACgg                          >  1:505581/1‑67 (MQ=255)                                        gggCGCACAAGCCAACAGTGAGGAGGGCTGGACGCTGATCATGCCTGACTGGCAAAACGGGGAAGgcgc                 <  1:99022/69‑1 (MQ=255)                                        gggCGCACAAGCCAACAGTGAGGAGGGCTGGACGCTGATCATGCCTGACTGGCAAAACGGGGAAGgcgc                 <  1:2976427/69‑1 (MQ=255)                                        gggCGCACAAGCCAACAGTGAGGAGGGCTGGACGCTGATCATGCCTGACTGGCAAAACGGGGAAGgcgc                 <  1:481414/69‑1 (MQ=255)                                        gggCGCACAAGCCAACAGTGAGGAGGGCTGGACGCTGATCATGCCTGACTGGCAAAACGGGGAAGgcgc                 <  1:689717/69‑1 (MQ=255)                                        gggCGCACAAGCCAACAGTGAGGAGGGCTGGACGCTGATCATGCCTGACTGGCAAAACGGGGAAGgcgc                 <  1:736510/69‑1 (MQ=255)                                        gggCGCACAAGCCAACAGTGAGGAGGGCTGGACGCTGATCATGCCTGACTGGCAAAACGGGGAAGgcgc                 <  1:739380/69‑1 (MQ=255)                                        gggCGCACAAGCCAACAGTGAGGAGGGCTGGACGCTGATCATGCCTGACTGGCAAAACGGGGAAGgcgc                 <  1:805196/69‑1 (MQ=255)                                        gggCGCACAAGCCAACAGTGAGGAGGGCTGGACGCTGATCATGCCTGACTGGCAAAACGGGGAAGgcgc                 <  1:888520/69‑1 (MQ=255)                                        gggCGCACAAGCCAACAGTGAGGAGGGCTGGACGCTGATCATGCCTGACTGGCAAAACGGGGAAGgcgc                 <  1:1173152/69‑1 (MQ=255)                                        gggCGCACAAGCCAACAGTGAGGAGGGCTGGACGCTGATCATGCCTGACTGGCAAAACGGGGAAGgcgc                 <  1:1187586/69‑1 (MQ=255)                                        gggCGCACAAGCCAACAGTGAGGAGGGCTGGACGCTGATCATGCCTGACTGGCAAAACGGGGAAGgcgc                 <  1:1337415/69‑1 (MQ=255)                                        gggCGCACAAGCCAACAGTGAGGAGGGCTGGACGCTGATCATGCCTGACTGGCAAAACGGGGAAGgcgc                 <  1:2080192/69‑1 (MQ=255)                                        gggCGCACAAGCCAACAGTGAGGAGGGCTGGACGCTGATCATGCCTGACTGGCAAAACGGGGAAGgcgc                 <  1:193133/69‑1 (MQ=255)                                        gggCGCACAAGCCAACAGTGAGGAGGGCTGGACGCTGATCATGCCTGACTGGCAAAACGGGGAAGgcgc                 <  1:181466/69‑1 (MQ=255)                                        gggCGCACAAGCCAACAGTGAGGAGGGCTGGACGCTGATCATGCCTGACTGGCAAAACGGGGAAGgcgc                 <  1:2491143/69‑1 (MQ=255)                                        gggCGCACAAGCCAACAGTGAGGAGGGCTGGACGCTGATCATGCCTGACTGGCAAAACGGGGAAGgcgc                 <  1:15820/69‑1 (MQ=255)                                        gggCGCACAAGCCAACAGTGAGGAGGGCTGGACGCTGATCATGCCTGACTGGCAAAACGGGGAAGgcgc                 <  1:1462277/69‑1 (MQ=255)                                         ggCGCACAAGCCAACAGTGAGGAGGGCTGGACGCTGATCATGCCTGACTGGCAAAACGGGGAAGgcgc                 <  1:501578/68‑1 (MQ=255)                                         ggCGCACAAGCCAACAGTGAGGAGGGCTGGACGCTGATCATGCCTGACTGGCAAAACGGGGAAGgcgc                 <  1:1443295/68‑1 (MQ=255)                                               caAGCCAACAGTGAGGAGGGCTGGACGCTGATCATGCCTGACTGGCAAAACGGGGAAGgcgc                 <  1:400362/62‑1 (MQ=255)                                                       cAGCGCGGAGGGCTGGACGCTGATCATGCCTGACTGGCAGAACGGGGAAGGGGCAAGCAATCACTGGCg  <  1:168860/69‑1 (MQ=255)                                                       cAGCGCGGAGGGCTGGACGCTGATCATGCCTGACTGGCAGAACGGGGAAGGGGCAAGCAATCACTGGCg  <  1:1022221/69‑1 (MQ=255)                                                       cAGCGCGGAGGGCTGGACGCTGATCATGCCTGACTGGCAGAACGGGGAAGGGGCAAGCAATCACTGGCg  <  1:3368590/69‑1 (MQ=255)                                                       cAGCGCGGAGGGCTGGACGCTGATCATGCCTGACTGGCAGAACGGGGAAGGGGCAAGCAATCACTGGCg  <  1:2245266/69‑1 (MQ=255)                                                       cAGCGCGGAGGGCTGGACGCTGATCATGCCTGACTGGCAGAACGGGGAAGGGGCAAGCAATCACTGGCg  <  1:3091494/69‑1 (MQ=255)                                                       cAGCGCGGAGGGCTGGACGCTGATCATGCCTGACTGGCAGAACGGGGAAGGGGCAAGCAATCACTGGCg  <  1:2396266/69‑1 (MQ=255)                                                            |                                                                GATTTGGCAGGGCGATACGCAGATATTAAGTTTGACGCCGGGCGCACAAGCCAACAGCGCGGAGGGCTGGACGCTGATCATGCCTGACTGGCAGAACGGGGAAGGGGCAAGCAATCACTGGCG  >  minE/826617‑826739

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑