| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 831,492 | T→C | 15.1% | intergenic (+465/‑51) | narK → / → narG | nitrate/nitrite transporter/nitrate reductase 1, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 831,492 | 0 | T | C | 15.1% | 75.3 / 4.6 | 46 | intergenic (+465/‑51) | narK/narG | nitrate/nitrite transporter/nitrate reductase 1, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base T (26/13); new base C (4/3); total (30/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.81e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.55e-01 | |||||||||||
CCCCATCACTCTTGATCGTTATCAATTCCCACGCTGTTTCAGAGCGTTACCTTGCCCTTAAACATTAGCAATGTCGATTTATCAGAGGGCCGACAGGCTCCCACAGGAGAAAACCGATGAGTAAATT > minE/831427‑831553 | ccccATCACTCTTGATCGTTATCAATTCCCACGCTGTTTCAGAGCGTTACCTTGCCCTTAAACATTAGc < 1:1105379/69‑1 (MQ=255)ccccATCACTCTTGATCGTTATCAATTCCCACGCTGTTTCAGAGCGTTACCTTGCCCTTAAACATTAGc < 1:839195/69‑1 (MQ=255)ccccATCACTCTTGATCGTTATCAATTCCCACGCTGTTTCAGAGCGTTACCTTGCCCTTAAACATTAGc < 1:765831/69‑1 (MQ=255)ccccATCACTCTTGATCGTTATCAATTCCCACGCTGTTTCAGAGCGTTACCTTGCCCTTAAACATTAGc < 1:2621705/69‑1 (MQ=255)ccccATCACTCTTGATCGTTATCAATTCCCACGCTGTTTCAGAGCGTTACCTTGCCCTTAAACATTAGc < 1:1320065/69‑1 (MQ=255)ccccATCACTCTTGATCGTTATCAATTCCCACGCTGTTTCAGAGCGTTACCTTGCCCTTAAACATTAGc < 1:1157931/69‑1 (MQ=255)ccccATCACTCTTGATCGTTATCAATTCCCACGCTGTTTCAGAGCGTTACCTTGCCCATAAACATTAGc < 1:770254/69‑1 (MQ=255) cACTCTTGATCGTTATCAATTCCCACGCTGTTTCAGAGCGTTACCTTGCCCTTAAACATTAGCaa > 1:669979/1‑65 (MQ=255) cACTCTTGATCGTTATCAATTCCCACGCTGTTTCAGAGCGTTACCTTGCCCTTAAACATTAGCaa > 1:2562072/1‑65 (MQ=255) cACTCTTGATCGTTATCAATTCCCACGCTGTTTCAGAGCGTTACCTTGCCCTTAAACATTAGCaa > 1:2242704/1‑65 (MQ=255) cACTCTTGATCGTTATCAATTCCCACGCTGTTTCAGAGCGTTACCTTGCCCTTAAACATTAGCaa > 1:2418872/1‑65 (MQ=255) cACTCTTGATCGTTATCAATTCCCACGCTGTTTCAGAGCGTTACCTTGCCCTTAAACATTAGCaa > 1:3339636/1‑65 (MQ=255) aCTCTTGATCGTTATCAATTCCCACGCTGTTTCAGAGCGTTACCTTGCCCTTAAACATTAGCAATGTCg > 1:3360360/1‑69 (MQ=255) tCAATTCCCACGCTGTTTCAGAGCGTTACCTTGCCCTTAAACATTAGCAATGTCGa > 1:1793583/1‑56 (MQ=255) tCAATTCCCACGCTGTTTCAGAGCGTTACCTTGCCCTTAAACATTAGCAATGTCGATTTATCAGAggg > 1:2767337/1‑68 (MQ=255) tCAATTCCCACGCTGTTTCAGAGCGTTACCTTGCCCTTAAACATTAGCAATGTCGATTTATCAGAggg > 1:322852/1‑68 (MQ=255) tCAATTCCCACGCTGTTTCAGAGCGTTACCTTGCCCTTAAACATTAGCAATGTCGATTTATCAGAggg > 1:1405530/1‑68 (MQ=255) tCAATTCCCACGCTGTTTCAGAGCGTTACCTTGCCCTTAAACATTAGCAATGTCGATTTATCAGAggg > 1:671993/1‑68 (MQ=255) tCAATTCCCACGCTGTTTCAGAGCGTTACCTTGCCCTTAAACATTAGCAATGTCGATTTATCAGAggg > 1:2373698/1‑68 (MQ=255) cAATTCCCACGCTGTTTCAGCGCGTTACCTTGCCCTTAAACATTAGCAATGTCGAttt > 1:1283298/1‑58 (MQ=255) cAATTCCCACGCTGTTTCAGAGCGTTACCTTGCCCTTAAACATTAGCAATGTCGAttt > 1:3402179/1‑58 (MQ=255) cAATTCCCACGCTGTTTCAGAGCGTTACCTTGCCCTTAAACATTAGCAATGTCGAttt > 1:331870/1‑58 (MQ=255) cAATTCCCACGCTGTTTCAGAGCGTTACCTTGCCCTTAAACATTAGCAATGTCGAttt > 1:569375/1‑58 (MQ=255) cAATTCCCACGCTGTTTCAGAGCGTTACCTTGCCCTTAAACATTAGCAATGTCGAttt > 1:1100638/1‑58 (MQ=255) cAATTCCCACGCTGTTTCAGAGCGTTACCTTGCCCTTAAACATTAGCAATGTCGAttt > 1:3153206/1‑58 (MQ=255) cAATTCCCACGCTGTTTCAGAGCGTTACCTTGCCCTTAAACATTAGCAATGTCGAttt > 1:2947076/1‑58 (MQ=255) cAATTCCCACGCTGTTTCAGAGCGTTACCTTGCCCTTAAACATTAGCAATGTCGAttt > 1:1335786/1‑58 (MQ=255) cAATTCCCACGCTGTTTCAGAGCGTTACCTTGCCCTTAAACATTAGCAATGTCGAttt > 1:2717475/1‑58 (MQ=255) cAATTCCCACGCTGTTTCAGAGCGTTACCTTGCCCTTAAACATTAGCAATGTCGAttt > 1:2626410/1‑58 (MQ=255) cAATTCCCACGCTGTTTCAGAGCGTTACCTTGCCCTTAAACATTAGCAATGTCGAttt > 1:1250225/1‑58 (MQ=255) cAATTCCCACGCTGTTTCAGAGCGTTACCTTGCCCTTAAACATTAGCAATGTCGAttt > 1:2474365/1‑58 (MQ=255) cAATTCCCACGCTGTTTCAGAGCGTTACCTTGCCCTTAAACATTAGCAATGTCGAttt > 1:2275491/1‑58 (MQ=255) cAATTCCCACGCTGTTTCAGAGCGTTACCTTGCCCTTAAACATTAGCAATGTCGAttt > 1:2077029/1‑58 (MQ=255) cccACGCTGTTTCAGAGCGTTACCTTGCCCTTAAACATCAGCAATGTCGATTTATCAGAGGGCCGACAg > 1:1338329/1‑69 (MQ=255) cccACGCTGTTTCAGAGCGTTACCTTGCCCTTAAACATCAGCAATGTCGATTTATCAGAGGGCCGACAg > 1:1514420/1‑69 (MQ=255) cccACGCTGTTTCAGAGCGTTACCTTGCCCTTAAACATCAGCAATGTCGATTTATCAGAGGGCCGACAg > 1:2261098/1‑69 (MQ=255) cccACGCTGTTTCAGAGCGTTACCTTGCCCTTAAACATCAGCAATGTCGATTTATCAGAGGGCCGACAg > 1:749773/1‑69 (MQ=255) ccTTAAACATTAGCAATGTCGATTTATCAGAGGGCCGACAGGCTCCCACAGGAGAAAACCGATGAGTa < 1:893024/68‑1 (MQ=255) ccTTAAACATTAGCAATGTCGATTTATCAGAGGGCCGACAGGCTCCCACAGGAGAAAACCGATGAGTa < 1:1545139/68‑1 (MQ=255) ttAAACATCAGCAATGTCGATTTATCAGAGGGCCGACAGGCTCCCACAGGAGAAAACCGATGAGTAAAt < 1:2250242/69‑1 (MQ=255) ttAAACATCAGCAATGTCGATTTATCAGAGGGCCGACAGGCTCCCACAGGAGAAAACCGATGAGTAAAt < 1:3061013/69‑1 (MQ=255) tcaaaCATCAGCAATGTCGATTTATCAGAGGCCCGACAGGCTCCCACAGGAGAAAACCGATGAGTAAAt < 1:3230210/67‑1 (MQ=255) tAAACATTAGCAATGTCGATTTATCAGAGGGCCGACAGGCTCCCACAGGAGAAAACCGATGAGTAAAtt < 1:194405/69‑1 (MQ=255) tAAACATTAGCAATGTCGATTTATCAGAGGGCCGACAGGCTCCCACAGGAGAAAACCGATGAGTAAAtt < 1:1586028/69‑1 (MQ=255) tAAACATTAGCAATGTCGATTTATCAGAGGGCCGACAGGCTCCCACAGGAGAAAACCGATGAGTAAAtt < 1:3100885/69‑1 (MQ=255) tAAACATTAGCAATGTCGATTTATCAGAGGGCCGACAGGCTCCCACAGGAGAAAACCGATGAGTAAAtt < 1:298327/69‑1 (MQ=255) | CCCCATCACTCTTGATCGTTATCAATTCCCACGCTGTTTCAGAGCGTTACCTTGCCCTTAAACATTAGCAATGTCGATTTATCAGAGGGCCGACAGGCTCCCACAGGAGAAAACCGATGAGTAAATT > minE/831427‑831553 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |