| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 832,367 | G→A | 48.5% | G275G (GGG→GGA) | narG → | nitrate reductase 1, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 832,367 | 0 | G | A | 48.5% | 4.2 / 93.4 | 105 | G275G (GGG→GGA) | narG | nitrate reductase 1, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base G (26/28); new base A (34/17); total (60/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.57e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 105 total aligned reads displayed.
AAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACT > minE/832307‑832414 | aaaCGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGAGCAAAAc < 1:6115/69‑1 (MQ=255)aaaCGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAAc < 1:2088056/69‑1 (MQ=255)aaaCGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAAc < 1:2062863/69‑1 (MQ=255)aaaCGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAAc < 1:909060/69‑1 (MQ=255)aaaCGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAAc < 1:1243684/69‑1 (MQ=255)aaaCGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAAc < 1:840286/69‑1 (MQ=255)aaaCGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAAc < 1:686738/69‑1 (MQ=255)aaaCGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAAc < 1:524416/69‑1 (MQ=255) tGCCGCAGACGCGTACCCCGGATGCTCTCTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTg > 1:3305942/1‑69 (MQ=255) tGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTTCAAAGGGACCAAAACTGtt > 1:2454683/1‑68 (MQ=255) tGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTg > 1:2033709/1‑69 (MQ=255) tGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTg > 1:3124900/1‑69 (MQ=255) gCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGt < 1:977691/69‑1 (MQ=255) gCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGt < 1:1932340/69‑1 (MQ=255) gCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGt < 1:2475726/69‑1 (MQ=255) gCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGt < 1:2532552/69‑1 (MQ=255) gCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGt < 1:750612/69‑1 (MQ=255) gCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGt < 1:1444193/69‑1 (MQ=255) acAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGt < 1:827331/68‑1 (MQ=255) aGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTca < 1:1194807/69‑1 (MQ=255) aGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTca < 1:349534/69‑1 (MQ=255) aGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTca < 1:2028946/69‑1 (MQ=255) aGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGCTGCCGTca < 1:2056573/69‑1 (MQ=255) gACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTcac < 1:1599003/69‑1 (MQ=255) gACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTcac < 1:1707574/69‑1 (MQ=255) gcgTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGAACCAAAACTGTTGCCGTcac > 1:2673479/1‑66 (MQ=255) gcgTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGAACCAAAACTGTTGCCGTcac > 1:2750783/1‑66 (MQ=255) gcgTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGAACCAAAACTGTTGCCGTcac > 1:365146/1‑66 (MQ=255) gcgTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGAACCAAAACTGTTGCCGTcac > 1:2796466/1‑66 (MQ=255) gcgTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGAACCAAAACTGTTGCCGTcac > 1:2119523/1‑66 (MQ=255) gcgTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGAACCAAAACTGTTGCCGTcac > 1:2888164/1‑66 (MQ=255) gcgTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGAACCAAAACTGTTGCCGTcac > 1:854136/1‑66 (MQ=255) gcgTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGAACCAAAACTGTTGCCGTcac > 1:2902869/1‑66 (MQ=255) gcgTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGAACCAAAACTGTTGCCGTcac > 1:3252924/1‑66 (MQ=255) gcgTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGAACCAAAACTGTTGCCGTcac > 1:2739729/1‑66 (MQ=255) gcgTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGAACCAAAACTGTTGCCGTcac > 1:1344615/1‑66 (MQ=255) gcgTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGAACCAAAACTGTTGCCGTcac > 1:1423912/1‑66 (MQ=255) gcgTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGAACCAAAACTGTTGCCGTcac > 1:1516648/1‑66 (MQ=255) gcgTACCCCGGATGATCACTTCTTTACTGAAGTGCGTTACAAAGGAACCAAAACTGTTGCCGTcac > 1:1441239/1‑66 (MQ=255) tGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGa > 1:1292526/1‑69 (MQ=255) tcttTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTa > 1:1419748/1‑55 (MQ=255) tcttTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCa > 1:1768132/1‑69 (MQ=255) tcttTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCa > 1:2089889/1‑69 (MQ=255) tcttTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCa > 1:33358/1‑69 (MQ=255) tcttTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCa > 1:1467892/1‑69 (MQ=255) tcttTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCa > 1:2539714/1‑69 (MQ=255) tcttTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCa > 1:1782922/1‑69 (MQ=255) tcttTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCa > 1:1409941/1‑69 (MQ=255) cttTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCaa > 1:379062/1‑69 (MQ=255) cttTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCaa > 1:1782694/1‑69 (MQ=255) cttTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCaa > 1:755842/1‑69 (MQ=255) cttTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCaa > 1:1656404/1‑69 (MQ=255) cttTACTGAAGTGCGTTACAAAGGAACCAAAACTGTTGCCGt > 1:3305869/1‑42 (MQ=255) cttTACTGAAGTGCGTTACAAAGGAACCAAAACTGTTGCCGTCACAcc > 1:791005/1‑48 (MQ=255) cttTACTGAAGTGCGTTACAAAGGAACCAAAACTGTTGCCGTCACACCa > 1:398957/1‑49 (MQ=255) cttTACTGAAGTGCGTTACAAAGGAACCAAAACTGTTGCCGTCACACCa > 1:1357882/1‑49 (MQ=255) cttTACTGAAGTGCGTTACAAAGGAACCAAAACTGTTGCCGTCACACCa > 1:416831/1‑49 (MQ=255) cttTACTGAAGTGCGTTACAAAGGAACCAAAACTGTTGCCGTCACACCa > 1:477813/1‑49 (MQ=255) cttTACTGAAGTGCGTTACAAAGGAACCAAAACTGTTGCCGTCACACCa > 1:75388/1‑49 (MQ=255) cttTACTGAAGTGCGTTACAAAGGAACCAAAACTGTTGCCGTCACACCa > 1:788153/1‑49 (MQ=255) cttTACTGAAGTGCGTTACAAAGGAACCAAAACTGTTGCCGTCACACCa > 1:2141559/1‑49 (MQ=255) cttTACTGAAGTGCGTTACAAAGGAACCAAAACTGTTGCCGTCACACCa > 1:2238312/1‑49 (MQ=255) cttTACTGAAGTGCGTTACAAAGGAACCAAAACTGTTGCCGTCACACCa > 1:2348460/1‑49 (MQ=255) cttTACTGAAGTGCGTTACAAAGGAACCAAAACTGTTGCCGTCACACCa > 1:9678/1‑49 (MQ=255) cttTACTGAAGTGCGTTACAAAGGAACCAAAACTGTTGCCGTCACACCa > 1:2556660/1‑49 (MQ=255) cttTACTGAAGTGCGTTACAAAGGAACCAAAACTGTTGCCGTCACACCa > 1:2810869/1‑49 (MQ=255) cttTACTGAAGTGCGTTACAAAGGAACCAAAACTGTTGCCGTCACACCa > 1:2650034/1‑49 (MQ=255) cttTACTGAAGTGCGTTACAAAGGAACCAAAACTGTTGCCGTCACACCa > 1:270589/1‑49 (MQ=255) cttTACTGAAGTGCGTTACAAAGGAACCAAAACTCTTGCCGTCACACCa > 1:3169715/1‑49 (MQ=255) cttTACT‑AAGTGCGTTACAAAGGAACCAAAACTGTTGCCGTCACACCa > 1:2680167/1‑48 (MQ=255) cattACTGAAGTGCGTTACAAAGGAACCAAAACTGTTGCCGTCACACCa > 1:1206365/3‑49 (MQ=255) tACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACt > 1:2926200/1‑69 (MQ=255) tACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACt > 1:1495762/1‑69 (MQ=255) tACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACt > 1:1717963/1‑69 (MQ=255) tACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACt > 1:1663495/1‑69 (MQ=255) tACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACt > 1:851308/1‑69 (MQ=255) tACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACt > 1:2885438/1‑69 (MQ=255) tACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACt > 1:2946457/1‑69 (MQ=255) aGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACt < 1:385741/51‑1 (MQ=255) aGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACt < 1:1799617/51‑1 (MQ=255) aGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACt < 1:2936338/51‑1 (MQ=255) aGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACt < 1:1384482/51‑1 (MQ=255) aGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACt < 1:1086417/51‑1 (MQ=255) aGGAACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAAt < 1:878576/42‑1 (MQ=255) aGGAACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAAt < 1:3176624/42‑1 (MQ=255) aGGAACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAAt < 1:614783/42‑1 (MQ=255) aGGAACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAAt < 1:60994/42‑1 (MQ=255) aGGAACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAAt < 1:598878/42‑1 (MQ=255) aGGAACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAAt < 1:5479/42‑1 (MQ=255) aGGAACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAAt < 1:3227062/42‑1 (MQ=255) aGGAACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAAt < 1:1605735/42‑1 (MQ=255) aGGAACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAAt < 1:2982893/42‑1 (MQ=255) aGGAACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAAt < 1:1609619/42‑1 (MQ=255) aGGAACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAAt < 1:2828730/42‑1 (MQ=255) aGGAACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAAt < 1:2811246/42‑1 (MQ=255) aGGAACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAAt < 1:2780923/42‑1 (MQ=255) aGGAACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAAt < 1:2760470/42‑1 (MQ=255) aGGAACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAAt < 1:1002081/42‑1 (MQ=255) aGGAACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAAt < 1:1994566/42‑1 (MQ=255) aGGAACCAAAACTGTTGACGTCACACCAGACTACGCTGAAAt < 1:2357290/42‑1 (MQ=255) | AAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACT > minE/832307‑832414 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |