| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 832,634 | G→A | 22.2% | A364A (GCG→GCA) | narG → | nitrate reductase 1, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 832,634 | 0 | G | A | 22.2% | 92.2 / 17.6 | 67 | A364A (GCG→GCA) | narG | nitrate reductase 1, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base G (3/49); new base A (6/9); total (9/58) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.79e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.06e-01 | |||||||||||
GATGCTGGAAGAACGCGACGGTTACTACGCTGCAGGTCGTATGCTGCGCGCTGCTGATCTGGTTGATGCGCTGGGCCAGGAAAACAATCCGGAATGGAAAACTGTCGCCTTTAATACCAATGGCGA > minE/832565‑832690 | gATGCTGGAAGAACGCGACGGTTACTACGCTGCAGGTCGTATGCTGCGCGCTGCT‑ATCTGGTTGATgcg > 1:598906/1‑69 (MQ=255) actacGCTGCAGGTCGTATGCTGCGCGCTGCTGATCTGGTTGATGCGCTGGGCCAGGAAAACAATCCgg < 1:1740973/69‑1 (MQ=255) actacGCTGCAGGTCGTATGCTGCGCGCTGCTGATCTGGTTGATGCGCTGGGCCAGGAAAACAATCCgg < 1:1803660/69‑1 (MQ=255) actacGCTGCAGGTCGTATGCTGCGCGCTGCTGATCTGGTTGATGCGCTGGGCCAGGAAAACAATCCgg < 1:1848740/69‑1 (MQ=255) actacGCAGCAGGTCGTATGCTGCGCGCTGCTGATCTGGTTGATGCGCTGGGCCAGGAAAACAATCCgg < 1:773562/69‑1 (MQ=255) tacGCTGCAGGTCGTATGCTGCGCGCTGCTGATCTGGTTGATGCGCTGGGCCAGGAAAACAATCCgg < 1:767428/67‑1 (MQ=255) gCAGGTCGTATGCTGCGCGCTGCTGATCTGGTTGATGCGCTGGGCCAGGAAAACAATCCgg < 1:1855441/61‑1 (MQ=255) gCAGGTCGTATGCTGCGCGCTGCTGATCTGGTTGATGCGCTGGGCCAGGAAAACAATCCGGAATGGaaa < 1:874646/69‑1 (MQ=255) ggTCGTATGCTGCGCGCTGCTGATCTGGTTGATGCGCTGGGCCAGGAAAACAATCCGGAATGGAAAACt < 1:1298182/69‑1 (MQ=255) ggTCGTATGCTGCGCGCTGCTGATCTGGTTGATGCGCTGGGCCAGGAAAACAATCCGGAATGGAAAACt < 1:2051810/69‑1 (MQ=255) ggTCGTATGCTGCGCGCTGCTGATCTGGTTGATGCGCTGGGCCAGGAAAACAATCCGGAATGGAAAACt < 1:1260111/69‑1 (MQ=255) ggTCGTATGCTGCGCGCTGCTGATCTGGTTGATGCGCTGGGCCAGGAAAACAATCCGGAATGGAAAACt < 1:2082442/69‑1 (MQ=255) ggTCGTATGCTGCGCGCTGCTGATCTGGTTGATGCGCTGGGCCAGGAAAACAATCCGGAATGGAAAACt < 1:3326041/69‑1 (MQ=255) ggTCGTATGCTGCGCGCTGCTGATCTGGTTGATGCGCTGGGCCAGGAAAACAATCCGGAATGGAAAACt < 1:1641068/69‑1 (MQ=255) ggTCGTATGCTGCGCGCTGCTGATCTGGTTGATGCGCTGGGCCAGGAAAACAATCCGGAATGGAAAACt < 1:2658406/69‑1 (MQ=255) ggTCGTATGCTGCGCGCTGCTGATCTGGTTGATGCGCTGGGCCAGGAAAACAATCCGGAATGGAAAACt < 1:2108312/69‑1 (MQ=255) tgctgcGCGCTGCTGATCTGGTTGATGCGCTGGGCTAGGAAAACAATCCGGAAt < 1:2931530/54‑1 (MQ=255) tgctgcGCGCTGCTGATCTGGTTGATGCGCTGGGCCAGGAAAACAATCCGGAAt > 1:2682063/1‑54 (MQ=255) tgctgcGCGCTGCTGATCTGGTTGATGCGCTGGGCCAGGAAAACAATCCGGAAt < 1:1054/54‑1 (MQ=255) tgctgcGCGCTGCTGATCTGGTTGATGCGCTGGGCCAGGAAAACAATCCGGAAt < 1:2718345/54‑1 (MQ=255) tgctgcGCGCTGCTGATCTGGTTGATGCGCTGGGCCAGGAAAACAATCCGGAAt < 1:672036/54‑1 (MQ=255) tgctgcGCGCTGCTGATCTGGTTGATGCGCTGGGCCAGGAAAACAATCCGGAAt < 1:2786182/54‑1 (MQ=255) tgctgcGCGCTGCTGATCTGGTTGATGCGCTGGGCCAGGAAAACAATCCGGAAt < 1:3269638/54‑1 (MQ=255) tgctgcGCGCTGCTGATCTGGTTGATGCGCTGGGCCAGGAAAACAATCCGGAAt < 1:501272/54‑1 (MQ=255) tgctgcGCGCTGCTGATCTGGTTGATGCGCTGGGCCAGGAAAACAATCCGGAAt < 1:360520/54‑1 (MQ=255) tgctgcGCGCTGCTGATCTGGTTGATGCGCTGGGCCAGGAAAACAATCCGGAAt < 1:475666/54‑1 (MQ=255) tgctgcGCGCTGCTGATCTGGTTGATGCGCTGGGCCAGGAAAACAATCCGGAAt > 1:2202315/1‑54 (MQ=255) tgctgcGCGCTGCTGATCTGGTTGATGCGCTGGGCCAGGAAAACAATCCGGAAt < 1:1749417/54‑1 (MQ=255) tgctgcGCGCTGCTGATCTGGTTGATGCGCTGGGCCAGGAAAACAATCCGGAAt < 1:934493/54‑1 (MQ=255) tgctgcGCGCTGCTGATCTGGTTGATGCGCTGGGCCAGGAAAACAATCCGGAAt < 1:1626137/54‑1 (MQ=255) tgctgcGCGCTGCTGATCTGGTTGATGCGCTGGGCCAGGAAAACAATCCGGAAt < 1:1258142/54‑1 (MQ=255) tgctgcGCGCTGATGATCTGGTTGATGCGCTGGGCCAGGAAAACAATCCGGAAt > 1:61823/1‑54 (MQ=255) gcgcgcTGCTGATCTGGTTGATGCGCTGGGCCAGGAAAACAATCCGGAATGGAAAACTGTCGCCTTTaa < 1:115349/69‑1 (MQ=255) gcgcgcTGCTGATCTGGTTGATGCGCTGGGCCAGGAAAACAATCCGGAATGGAAAACTGTCGCCTTTaa < 1:2594031/69‑1 (MQ=255) gcgcgcTGCTGATCTGGTTGATGCGCTGGGCCAGGAAAACAATCCGGAATGGAAAACTGTCGCCTTTaa < 1:142769/69‑1 (MQ=255) gcgcgcTGCTGATCTGGTTGATGCGCTGGGCCAGGAAAACAATCCGGAATGGAAAACTGTCGCCTTTaa < 1:1356712/69‑1 (MQ=255) gcgcgcTGCTGATCTGGTGGATGCACTGGGCCAGGAAAACAATCCGGAATGGAAAACTGTCGCCtttga < 1:1326384/69‑3 (MQ=255) gcgcgcTGCTGATCTGGTGGATGCACTGGGCCAGGAAAACAATCCGGAATGGAAAACTGTCGCCTTTaa < 1:779403/69‑1 (MQ=255) gcgcgcTGCTGATCTGGTGGATGCACTGGGCCAGGAAAACAATCCGGAATGGAAAACTGTCGCCTTTaa < 1:3331655/69‑1 (MQ=255) gcgcgcTGCTGATCTGGTGGATGCACTGGGCCAGGAAAACAATCCGGAATGGAAAACTGTCGCCTTTaa < 1:1220021/69‑1 (MQ=255) gcgcgcTGCTGATCTGGTGGATGCACTGGGCCAGGAAAACAATCCGGAATGGAAAACTGTCGCCTTTaa < 1:3166844/69‑1 (MQ=255) gcgcgcTGCTGATCTGGTGGATGCACTGGGCCAGGAAAACAATCCGGAATGGAAAACTGTCGCCTTTaa < 1:614296/69‑1 (MQ=255) gcgcgcTGCTGATCTGGTGGATGCACTGGGCCAGGAAAACAATCCGGAATGGAAAACTGTCGCCTTTaa < 1:1372138/69‑1 (MQ=255) gcgcgcTGCTGATCTGGTGGATGCACTGGGCCAGGAAAACAATCCGGAATGGAAAACTGTCGCCTTTaa < 1:914634/69‑1 (MQ=255) cgcgcTGCTGATCTGGTGGATGCACTGGGCCAGGAAAACAATCCGGAATGGAAAACTGTCGCCTTTaa < 1:1591980/68‑1 (MQ=255) gctgctGATCTGGTGGATGCACTGGGCCAGGAAAACAATCCGGAATGGAAAACTGTCGCCTTTAATAcc > 1:604717/1‑69 (MQ=255) gctgctGATCTGGTGGATGCACTGGGCCAGGAAAACAATCCGGAATGGAAAACTGTCGCCTTTAATAcc > 1:2913763/1‑69 (MQ=255) gctgctGATCTGGTGGATGCACTGGGCCAGGAAAACAATCCGGAATGGAAAACTGTCGCCTTTAATAcc > 1:480987/1‑69 (MQ=255) gctgctGATCTGGTGGATGCACTGGGCCAGGAAAACAATCCGGAATGGAAAACTGTCGCCTTTAATAcc > 1:21499/1‑69 (MQ=255) gctgctGATCTGGTGGATGCACTGGGCCAGGAAAACAATCCGGAATGGAAAACTGTCGCCTTTAATAcc > 1:472635/1‑69 (MQ=255) gctgctGATCTGGTGGATGCACTGGGCCAGGAAAACAATCCGGAATGGAAAACTGTCGCCTATAATAcc > 1:1113425/1‑69 (MQ=255) gctgATCTGGTTGATGCGCTGGGCCAGGAAAACAATCCGGAATGGAAAACTGTCGCCttt < 1:3412648/60‑1 (MQ=255) gctgATCTGGTTGATGCGCTGGGCCAGGAAAACAATCCGGAATGGAAAACTGTCGCCttt < 1:1172207/60‑1 (MQ=255) gctgATCTGGTTGATGCGCTGGGCCAGGAAAACAATCCGGAATGGAAAACTGTCGCCttt < 1:2453296/60‑1 (MQ=255) gctgATCTGGTTGATGCGCTGGGCCAGGAAAACAATCCGGAATGGAAAACTGTCGCCttt < 1:2532449/60‑1 (MQ=255) gctgATCTGGTTGATGCGCTGGGCCAGGAAAACAATCCGGAATGGAAAACTGTCGCCttt < 1:2572194/60‑1 (MQ=255) gctgATCTGGTTGATGCGCTGGGCCAGGAAAACAATCCGGAATGGAAAACTGTCGCCttt < 1:2718572/60‑1 (MQ=255) gctgATCTGGTTGATGCGCTGGGCCAGGAAAACAATCCGGAATGGAAAACTGTCGCCttt < 1:3001812/60‑1 (MQ=255) gctgATCTGGTTGATGCGCTGGGCCAGGAAAACAATCCGGAATGGAAAACTGTCGCCttt < 1:3027089/60‑1 (MQ=255) gctgATCTGGTTGATGCGCTGGGCCAGGAAAACAATCCGGAATGGAAAACTGTCGCCttt < 1:3228026/60‑1 (MQ=255) gctgATCTGGTTGATGCGCTGGGCCAGGAAAACAATCCGGAATGGAAAACTGTCGCCttt < 1:434449/60‑1 (MQ=255) cTGGTTGATGCGCTGGGCCAGGAAAACAATCCGGAATGGAAAACTGTCGCCTTTAATACCAATGGCGa < 1:64408/68‑1 (MQ=255) cTGGTTGATGCGCTGGGCCAGGAAAACAATCCGGAATGGAAAACTGTCGCCTTTAATACCAATGGCGa < 1:646898/68‑1 (MQ=255) cTGGTTGATGCGCTGGGCCAGGAAAACAATCCGGAATGGAAAACTGTCGCCTTTAATACCAATGGCGa < 1:3217169/68‑1 (MQ=255) cTGGTTGATGCGCTGGGCCAGGAAAACAATCCGGAATGGAAAACTGTCGCCTTTAATACCAATGGCGa < 1:3387324/68‑1 (MQ=255) cTGGTTGATGCGCTGGGCCAGGAAAACAATCCGGAATGGAAAACTGTCGCCTTTAATACCAATGGCGa < 1:496091/68‑1 (MQ=255) cTGGTTGATGCGCTGGGCCAGGAAAACAATCCGGAATGGAAAACTGTCGCCTTTAATACCAATGGCGa < 1:2227590/68‑1 (MQ=255) cTGGTTGATGCGCTGGGCCAGGAAAACAATCCGGAATGGAAAACTGTCGCCTTTAATACCAATGGCGa < 1:1735373/68‑1 (MQ=255) | GATGCTGGAAGAACGCGACGGTTACTACGCTGCAGGTCGTATGCTGCGCGCTGCTGATCTGGTTGATGCGCTGGGCCAGGAAAACAATCCGGAATGGAAAACTGTCGCCTTTAATACCAATGGCGA > minE/832565‑832690 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |