| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 834,062 | C→T | 51.8% | G840G (GGC→GGT) | narG → | nitrate reductase 1, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 834,062 | 0 | C | T | 51.8% | ‑2.2 / 72.8 | 81 | G840G (GGC→GGT) | narG | nitrate reductase 1, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base C (18/21); new base T (26/16); total (44/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.84e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.14e-01 | |||||||||||
AAGCGAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAA > minE/833998‑834122 | aaGCGAAAAGCGACTGTGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTTGGTCAt < 1:2179751/68‑1 (MQ=255)aaGCGAAAAGCGACTGGGAAATCTACAAAGCCATCGCTAAGAAATTCTCCGAAGTGTGCGTTGGTCAt < 1:373424/68‑1 (MQ=255)aaGCGAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTTGGTCAt < 1:1972254/68‑1 (MQ=255)aaGCGAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTTGGTCAt < 1:2002614/68‑1 (MQ=255)aaGCGAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTTGGTCAt < 1:1636959/68‑1 (MQ=255)aaGCGAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTTGGTCAt < 1:2572561/68‑1 (MQ=255)aaGCGAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTTGGTCAt < 1:3048937/68‑1 (MQ=255)aaGCGAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTTGGTCAt < 1:2517925/68‑1 (MQ=255)aaGCGAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTTGGTCAt < 1:144576/68‑1 (MQ=255)aaGCGAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTTGGTCAt < 1:3411540/68‑1 (MQ=255)aaGCGAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTTGGTCAt < 1:442802/68‑1 (MQ=255)aaGCGAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTTGGTCAt < 1:484687/68‑1 (MQ=255)aaGCGAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTTGGTCAt < 1:2459416/68‑1 (MQ=255)aaGCGAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTTGGTCAt < 1:827457/68‑1 (MQ=255)aaGCGAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTTGGTCAt < 1:2323190/68‑1 (MQ=255)aaGCGAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAACAAATTCTCCGAAGTGTGCGTTGGTCAt < 1:2515061/68‑1 (MQ=255) tCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTTGGTCATc > 1:3280213/1‑48 (MQ=255) tCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTTGGTCATCTGGGTAAAGAAAGTGACATc > 1:3391382/1‑68 (MQ=255) tCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTTGGTCATCTGGGTAAAGAAACTGACATc > 1:2616834/1‑68 (MQ=255) tCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTTGGTCATCTGGGTAAAGAAACTGACATc > 1:2341592/1‑68 (MQ=255) tCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTTGGTCATCTGGGTAAAGAAACTGACATc > 1:2800266/1‑68 (MQ=255) tCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTTGGTCATCTGGGTAAAGAAACTGACATc > 1:2908151/1‑68 (MQ=255) tCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTTGGTCATCTGGGTAAAGAAACTGACATc > 1:2880597/1‑68 (MQ=255) tCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTTGGTCATCTGGGTAAAGAAACTGACATc > 1:289969/1‑68 (MQ=255) tCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTTGGTCATCTGGGTAAAGAAACTGACATc > 1:2900156/1‑68 (MQ=255) tCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTTGGTCATCTGGGTAAAGAAACTGACATc > 1:938117/1‑68 (MQ=255) tCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTTGGTCATCTGGGTAAAGAAACTGACATc > 1:3155589/1‑68 (MQ=255) tCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTTGGTCATCTGGGTAAAGAAACTGACATc > 1:519947/1‑68 (MQ=255) tCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTTGGTCATCTGGGTAAAGAAACTGACATc > 1:808160/1‑68 (MQ=255) tCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTTGGTCATCTGGGTAAAGAAACTGACATc > 1:90610/1‑68 (MQ=255) tCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTTGGTCATCTGGGTAAAGAAACTGACATc > 1:934978/1‑68 (MQ=255) tCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTTGGTCATCTGGGTAAAGAAACTGACATc > 1:1057876/1‑68 (MQ=255) tCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTTGGTCATCTGGGTAAAGAAACTGACATc > 1:2088851/1‑68 (MQ=255) tCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTTGGTCATCTGGGTAAAGAAACTGACATc > 1:1173125/1‑68 (MQ=255) tCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTTGGTCATCTGGGTAAAGAAACTGACATc > 1:1196444/1‑68 (MQ=255) tCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTTGGTCATCTGGGTAAAGAAACTGACATc > 1:1371294/1‑68 (MQ=255) tCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTTGGTCATCTGGGTAAAGAAACTGACATc > 1:1462947/1‑68 (MQ=255) tCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTTGGTCATCTGGGTAAAGAAACTGACATc > 1:1897517/1‑68 (MQ=255) tCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTTGGTCATCTGGGTAAAGAAACTGACATc > 1:1973169/1‑68 (MQ=255) tCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTTGGTCATCTGGGTAAAGAAACTGACATc > 1:204195/1‑68 (MQ=255) tCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTTGGTCATCTGGGTAAAGAAACTGACATc > 1:2045779/1‑68 (MQ=255) tCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTAGGTCATCTGGGTAAAGAAACTGACATc > 1:1064542/1‑68 (MQ=255) cTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGt < 1:2550409/69‑1 (MQ=255) cTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGt < 1:1068048/69‑1 (MQ=255) cTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGt < 1:671584/69‑1 (MQ=255) cTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGt < 1:667372/69‑1 (MQ=255) cTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGt < 1:1740803/69‑1 (MQ=255) cTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGGCGGCCATCTGGGTAAAGAAACCGACATCGt < 1:3277857/69‑1 (MQ=255) gcgAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTa > 1:2111572/1‑67 (MQ=255) gcgAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTa > 1:792549/1‑67 (MQ=255) gcgAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTa > 1:599474/1‑67 (MQ=255) gcgAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTa > 1:1314562/1‑67 (MQ=255) gcgAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTa > 1:478556/1‑67 (MQ=255) gcgAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTa > 1:2060349/1‑67 (MQ=255) gcgAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTa > 1:1490452/1‑67 (MQ=255) gcgAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTa > 1:1581622/1‑67 (MQ=255) gcgAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTa > 1:2038018/1‑67 (MQ=255) gcgAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTa > 1:1596059/1‑67 (MQ=255) gcgAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTa > 1:2710378/1‑67 (MQ=255) gcgAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTa > 1:2457491/1‑67 (MQ=255) gcgAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTa > 1:2855102/1‑67 (MQ=255) gcgAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTa > 1:2857191/1‑67 (MQ=255) gcgAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTa > 1:2447417/1‑67 (MQ=255) aagaaATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAg < 1:204585/69‑1 (MQ=255) aagaaATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAg < 1:2965654/69‑1 (MQ=255) aagaaATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAg < 1:1333334/69‑1 (MQ=255) aagaaATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAg < 1:1578441/69‑1 (MQ=255) tgCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTg > 1:523068/1‑67 (MQ=255) tgCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGaa > 1:2262736/1‑69 (MQ=255) tgCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGaa > 1:2202709/1‑69 (MQ=255) cGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGcc < 1:45671/42‑1 (MQ=255) cGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGcc < 1:1349511/42‑1 (MQ=255) cGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGcc < 1:2087097/42‑1 (MQ=255) cGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGcc < 1:1223076/42‑1 (MQ=255) cGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGcc < 1:3407953/42‑1 (MQ=255) cGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGcc < 1:639227/42‑1 (MQ=255) cGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGcc < 1:3406009/42‑1 (MQ=255) cGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGcc < 1:1620945/42‑1 (MQ=255) cGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGcc < 1:294348/42‑1 (MQ=255) cGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGcc < 1:111287/42‑1 (MQ=255) cGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGcc < 1:2055849/42‑1 (MQ=255) | AAGCGAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAA > minE/833998‑834122 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |