| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 834,107 | T→C | 57.7% | H855H (CAT→CAC) | narG → | nitrate reductase 1, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 834,107 | 0 | T | C | 57.7% | 17.6 / 42.2 | 59 | H855H (CAT→CAC) | narG | nitrate reductase 1, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base T (15/10); new base C (20/14); total (35/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01 | |||||||||||
TGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAAAAAAGGCGAGTGCGACCTGA > minE/834054‑834174 | tgCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTg > 1:523068/1‑67 (MQ=255)tgCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGaa > 1:2262736/1‑69 (MQ=255)tgCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGaa > 1:2202709/1‑69 (MQ=255) aaGAAACTGACATCGTCACGCTGCCTATCCAGCACGACTCTGCCGCTGAACTg > 1:1035166/1‑53 (MQ=255) aaGAAACTGACATCGTCACGCTGCCTATCCAGCACGACTCTGCCGCTGAACTg > 1:2621894/1‑53 (MQ=255) aaGAAACTGACATCGTCACGCTGCCTATCCAGCACGACTCTGCCGCTGAACTg > 1:2447446/1‑53 (MQ=255) aaGAAACTGACATCGTCACGCTGCCTATCCAGCACGACTCTGCCGCTGAACTg > 1:2177150/1‑53 (MQ=255) aaGAAACTGACATCGTCACGCTGCCTATCCAGCACGACTCTGCCGCTGAACTg > 1:2841081/1‑53 (MQ=255) aaGAAACTGACATCGTCACGCTGCCTATCCAGCACGACTCTGCCGCTGAACTg > 1:2936416/1‑53 (MQ=255) aaGAAACTGACATCGTCACGCTGCCTATCCAGCACGACTCTGCCGCTGAACTg > 1:194809/1‑53 (MQ=255) aaGAAACTGACATCGTCACGCTGCCTATCCAGCACGACTCTGCCGCTGAACTg > 1:1941700/1‑53 (MQ=255) aaGAAACTGACATCGTCACGCTGCCTATCCAGCACGACTCTGCCGCTGAACTg > 1:3138525/1‑53 (MQ=255) aaGAAACTGACATCGTCACGCTGCCTATCCAGCACGACTCTGCCGCTGAACTg > 1:1836885/1‑53 (MQ=255) aaGAAACTGACATCGTCACGCTGCCTATCCAGCACGACTCTGCCGCTGAACTg > 1:1816368/1‑53 (MQ=255) aaGAAACTGACATCGTCACGCTGCCTATCCAGCACGACTCTGCCGCTGAACTg > 1:3194174/1‑53 (MQ=255) aaGAAACTGACATCGTCACGCTGCCTATCCAGCACGACTCTGCCGCTGAACTg > 1:1664688/1‑53 (MQ=255) aaGAAACTGACATCGTCACGCTGCCTATCCAGCACGACTCTGCCGCTGAACTg > 1:3229071/1‑53 (MQ=255) aaGAAACTGACATCGTCACGCTGCCTATCCAGCACGACTCTGCCGCTGAACTg > 1:1570834/1‑53 (MQ=255) aaGAAACTGACATCGTCACGCTGCCTATCCAGCACGACTCTGCCGCTGAACTg > 1:1080198/1‑53 (MQ=255) aaGAAACTGACATCGTCACGCTGCCTATCCAGCACGACTCTGCCGCTGAACTg > 1:908802/1‑53 (MQ=255) aaGAAACTGACATCGTCACGCTGCCTATCCAGCACGACTCTGCCGCTGAACTg > 1:591271/1‑53 (MQ=255) aaGAAACTGACATCGTCACGCTGCCTATCCAGCACGACTCTGCCGCTGAACTg > 1:837319/1‑53 (MQ=255) aaGAAACTGACATCGTCACGCTGCCTATCCAGCACGACTCTGCCGCTGAACTg > 1:1419337/1‑53 (MQ=255) aaaCCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGa < 1:978939/69‑1 (MQ=255) aaaCCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGa < 1:278906/69‑1 (MQ=255) aaCCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGAtgt > 1:530613/1‑66 (MQ=255) cGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGa < 1:308508/69‑1 (MQ=255) cGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGa < 1:2193603/69‑1 (MQ=255) gACATCGTCACGCTGCCTATCCAGCACGACTCTGCCGCTGAACTGGCGCAACCGCTGGATGTGAAAGAc < 1:1612252/69‑1 (MQ=255) gACATCGTCACGCTGCCTATCCAGCACGACTCTGCCGCTGAACTGGCGCAACCGCTGGATGTGAAAGAc < 1:2405481/69‑1 (MQ=255) gACATCGTCACGCTGCCTATCCAGCACGACTCTGCCGCTGAACTGGCGCAACCGCTGGATGTGAAAGAc < 1:2710147/69‑1 (MQ=255) gACATCGTCACGCTGCCTATCCAGCACGACTCTGCCGCTGAACTGGCGCAACCGCTGGATGTGAAAGAc < 1:2276909/69‑1 (MQ=255) gACATCGTCACGCTGCCTATCCAGCACGACTCTGCCGCTGAACTGGCGCAACCGCTGGATGTGAAAGAc < 1:1520932/69‑1 (MQ=255) gACATCGTCACGCTGCCTATCCAGCACGACTCTGCCGCTGAACTGGCGCAACCGCTGGATGTGAAAGAc < 1:317010/69‑1 (MQ=255) gACATCGTCACGCTGCCTATCCAGCACGACTCTGCCGCTGAACTGGCGCAACCGCTGGATGTGAAAGAc < 1:1833031/69‑1 (MQ=255) gACATCGTCACGCTGCCTAACCACCACGACTCTGCCGCTGAACTGGCGCAACCGCTGGATGTGAAAGAc < 1:1899343/69‑1 (MQ=255) cACGCTGCCTATCCAGCACGACTCTGCCGCTGAACTGGCGCAACCGCTGGATGTGAAAGAc < 1:1549700/61‑1 (MQ=255) tGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAAAAAAGGCg < 1:383702/69‑1 (MQ=255) tGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAAAAAAGGCg < 1:270032/69‑1 (MQ=255) tGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAAAAAAGGCg < 1:105807/69‑1 (MQ=255) tGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAAAAAAGGCg < 1:1932621/69‑1 (MQ=255) aTCCAGCACGACTCTGCCGCTGAACTGGCGCAACCGCTGGATGTGAATGAc < 1:902562/51‑1 (MQ=38) aTCCAGCACGACTCTGCCGCTGAACTGGCGCAACCGCTGGATGTGAAAGAc < 1:3328005/51‑1 (MQ=39) aTCCACCACGACTCTGCCGCTGAACTGGCGCAACCGCTGGATGTGAAAGAc < 1:262588/51‑1 (MQ=38) aTCCACCACGACTCTGCCGCTGAACTGGCGCAACCGCTGGATGTGAAAGAc < 1:3148449/51‑1 (MQ=38) ccAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAAAAAAGGc < 1:1548990/61‑1 (MQ=255) ccAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAAAAAAGGc > 1:3125758/1‑61 (MQ=255) ccAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAAAAAAGGc < 1:2003030/61‑1 (MQ=255) cAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGAc > 1:2445180/1‑48 (MQ=255) cAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAAAAAAGGCGAGTGCGAc > 1:860090/1‑69 (MQ=255) cAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAAAAAAGGCGAGTGCGAc > 1:134236/1‑69 (MQ=255) cAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAAAAAAGGCGAGTGCGAc > 1:3139990/1‑69 (MQ=255) cAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAAAAAAGGCGAGTGCGAc > 1:672852/1‑69 (MQ=255) cAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAAAAAAGGCGAGTGCGAc > 1:1495621/1‑69 (MQ=255) cAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAAAAAAGGCGAGTGCGAc > 1:2301947/1‑69 (MQ=255) cAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAAAAAAGGCGAGTGCGAc > 1:352087/1‑69 (MQ=255) cAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAAAAAAGGCGAGTGCGAc > 1:418746/1‑69 (MQ=255) gCACGACTCTGCCGCTGAACTGGCGCAACCGCTGGATGTGAAAGAc < 1:2173587/46‑1 (MQ=38) aTGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAAAAAAGGCGAGTGCGACCTGa > 1:3012109/1‑69 (MQ=255) tGACTCTGGCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAAAAAAGGCg > 1:1426121/1‑56 (MQ=255) tGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAAAAAAGGCg > 1:1293495/1‑56 (MQ=255) tGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAAAAAAGGCg > 1:1497909/1‑56 (MQ=255) tGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAAAAAAGGCg > 1:526202/1‑56 (MQ=255) tGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAAAAAAGGCg > 1:1526702/1‑56 (MQ=255) tGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAAAAAAGGCg > 1:1640857/1‑56 (MQ=255) tGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAAAAAAGGCg > 1:1731022/1‑56 (MQ=255) tGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAAAAAAGGCg > 1:1835201/1‑56 (MQ=255) tGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAAAAAAGGCg > 1:3083598/1‑56 (MQ=255) tGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAAAAAAGGCg > 1:3076164/1‑56 (MQ=255) tGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAAAAAAGGCg > 1:2015773/1‑56 (MQ=255) tGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAAAAAAGCCg > 1:2201807/1‑56 (MQ=255) | TGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAAAAAAGGCGAGTGCGACCTGA > minE/834054‑834174 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |