Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 834,674 A→G 42.8% Q1044Q (CAA→CAG narG → nitrate reductase 1, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE834,6740AG42.8% 7.2 / 29.5 42Q1044Q (CAA→CAGnarGnitrate reductase 1, alpha subunit
Reads supporting (aligned to +/- strand):  ref base A (11/13);  new base G (10/8);  total (21/21)
Fisher's exact test for biased strand distribution p-value = 7.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

CCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGG  >  minE/834616‑834723
                                                          |                                                 
ccGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAgg                                         <  1:1298452/69‑1 (MQ=255)
  ggTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGAt                                       <  1:1098044/69‑1 (MQ=255)
  ggTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGAt                                       <  1:416820/69‑1 (MQ=255)
  ggTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGAt                                       <  1:3261795/69‑1 (MQ=255)
  ggTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGAt                                       <  1:2019251/69‑1 (MQ=255)
  ggTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGAt                                       <  1:3056726/69‑1 (MQ=255)
  ggTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGAt                                       <  1:1891126/69‑1 (MQ=255)
   gTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGAt                                       <  1:2451720/68‑1 (MQ=255)
          cAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTg                               <  1:1455596/69‑1 (MQ=255)
          cAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTg                               <  1:150346/69‑1 (MQ=255)
                 cACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGt                        <  1:3211145/69‑1 (MQ=255)
                 cACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGt                        <  1:1704714/69‑1 (MQ=255)
                            cccGTGGCGTACGCTCTCTGGCCGTCAGCAGCTGTATCAGGATCAc                                    >  1:77332/1‑46 (MQ=38)
                            cccGTGGCGTACGCTCTCTGGCCGTCAGCAGCTGTATCAGGATCACCAATGGATGCGTGATTTCgg                >  1:2748044/1‑66 (MQ=255)
                            cccGTGGCGTACGCTCTCTGGCCGTCAGCAGCTGTATCAGGATCACCAATGGATGCGTGATTTCGGt               >  1:2567827/1‑67 (MQ=255)
                            cccGTGGCGTACGCTCTCTGGCCGTCAGCAGCTGTATCAGGATCACCAATGGATGCGTGATTTCGGt               >  1:2951117/1‑67 (MQ=255)
                            cccGTGGCGTACGCTCTCTGGCCGTCAGCAGCTGTATCAGGATCACCAATGGATGCGTGATTTCGGt               >  1:2454476/1‑67 (MQ=255)
                            cccGTGGCGTACGCTCTCTGGCCGTCAGCAGCTGTATCAGGATCACCAATGGATGCGTGATTTCGGt               >  1:3234926/1‑67 (MQ=255)
                            cccGTGGCGTACGCTCTCTGGCCGTCAGCAGCTGTATCAGGATCACCAATGGATGCGTGATTTCGGt               >  1:1069020/1‑67 (MQ=255)
                            cccGTGGCGTACGCTCTCTGGCCGTCAGCAGCTGTATCAGGATCACCAATGGATGCGTGATTTCGGt               >  1:2204985/1‑67 (MQ=255)
                            cccGTGGCGTACGCTCTCTGGCCGTCAGCAGCTGTATCAGGATCACCAATGGATGCGTGATTTCGGt               >  1:1433619/1‑67 (MQ=255)
                            cccGTGGCGTACGCTCTCTGGCCGTCAGCAGCTGTATCAGGATCACCAATGGATGCGTGATTTCGGt               >  1:1353817/1‑67 (MQ=255)
                            cccATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGt               >  1:2050459/1‑67 (MQ=255)
                            cccATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGt               >  1:1025308/1‑67 (MQ=255)
                            cccATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGt               >  1:763298/1‑67 (MQ=255)
                            cccATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGt               >  1:421434/1‑67 (MQ=255)
                            cccATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGt               >  1:1109549/1‑67 (MQ=255)
                            cccATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGt               >  1:2768016/1‑67 (MQ=255)
                            cccATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGt               >  1:2622360/1‑67 (MQ=255)
                            cccATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGt               >  1:1504995/1‑67 (MQ=255)
                                 ggCGTACGCTCTCTGGCCGTCAGCAGCTGTATCAGGATCACCAATGGATGCGTGATTTCGGTGAAAGcc        <  1:1398656/69‑1 (MQ=255)
                                 ggCGTACGCTCTCTGGCCGTCAGCAGCTGTATCAGGATCACCAATGGATGCGTGATTTCGGTGAAAGcc        <  1:1218414/69‑1 (MQ=255)
                                 ggCGTACGCTCTCTGGCCGTCAGCAGCTGTATCAGGATCACCAATGGATGCGTGATTTCGGTGAAAGcc        <  1:727599/69‑1 (MQ=255)
                                 ggCGTACGCTCTCTGGCCGTCAGCAGCTGTATCAGGATCACCAATGGATGCGTGATTTCGGTGAAAGcc        <  1:2553461/69‑1 (MQ=255)
                                 ggCGTACGCTCTCTGGCCGTCAGCAGCTGTATCAGGATCACCAATGGATGCGTGATTTCGGTGAAAGcc        <  1:1959281/69‑1 (MQ=255)
                                 ggCGTACGCTCTCTGGCCGTCAGCAGCTGTATCAGGATCACCAATGGATGCGTGATTTCGGTGAAAGcc        <  1:798643/69‑1 (MQ=255)
                                 ggCGTACGCTCTCTGGCCGTCAGCAGCTGTATCAGGATCACCAATGGATGCGTGATTTCGGTGAAAGcc        <  1:819558/69‑1 (MQ=255)
                                 ggCGTACGCTCTCTGGCCGTCAGCAGCTGTATCAGGATCACCAATGGATGCGTGATTTCGGTGAAAGcc        <  1:860637/69‑1 (MQ=255)
                                        gCTCTCTGGTCGTCAGCAACTGTATCAGGATCAGCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTgg  >  1:531865/1‑68 (MQ=255)
                                        gCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCct       >  1:1215407/1‑63 (MQ=255)
                                        gCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTgg  >  1:1370806/1‑68 (MQ=255)
                                                  cgtcAGCAACTGTATCAGGATCACCAGTGGATGCGt                        <  1:2857752/36‑1 (MQ=255)
                                                          |                                                 
CCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGG  >  minE/834616‑834723

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: