Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 834,692 G→A 30.8% Q1050Q (CAG→CAA narG → nitrate reductase 1, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE834,6920GA30.8% 50.1 / 23.1 55Q1050Q (CAG→CAAnarGnitrate reductase 1, alpha subunit
Reads supporting (aligned to +/- strand):  ref base G (30/8);  new base A (9/8);  total (39/16)
Fisher's exact test for biased strand distribution p-value = 6.19e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.83e-01

CAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACC  >  minE/834626‑834746
                                                                  |                                                      
cAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTg                                                      <  1:150346/69‑1 (MQ=255)
cAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTg                                                      <  1:1455596/69‑1 (MQ=255)
       cACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGt                                               <  1:3211145/69‑1 (MQ=255)
       cACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGt                                               <  1:1704714/69‑1 (MQ=255)
                  cccGTGGCGTACGCTCTCTGGCCGTCAGCAGCTGTATCAGGATCACCAATGGATGCGTGATTTCgg                                       >  1:2748044/1‑66 (MQ=255)
                  cccGTGGCGTACGCTCTCTGGCCGTCAGCAGCTGTATCAGGATCACCAATGGATGCGTGATTTCGGt                                      >  1:1069020/1‑67 (MQ=255)
                  cccGTGGCGTACGCTCTCTGGCCGTCAGCAGCTGTATCAGGATCACCAATGGATGCGTGATTTCGGt                                      >  1:2951117/1‑67 (MQ=255)
                  cccGTGGCGTACGCTCTCTGGCCGTCAGCAGCTGTATCAGGATCACCAATGGATGCGTGATTTCGGt                                      >  1:2567827/1‑67 (MQ=255)
                  cccGTGGCGTACGCTCTCTGGCCGTCAGCAGCTGTATCAGGATCACCAATGGATGCGTGATTTCGGt                                      >  1:3234926/1‑67 (MQ=255)
                  cccGTGGCGTACGCTCTCTGGCCGTCAGCAGCTGTATCAGGATCACCAATGGATGCGTGATTTCGGt                                      >  1:2454476/1‑67 (MQ=255)
                  cccGTGGCGTACGCTCTCTGGCCGTCAGCAGCTGTATCAGGATCACCAATGGATGCGTGATTTCGGt                                      >  1:2204985/1‑67 (MQ=255)
                  cccGTGGCGTACGCTCTCTGGCCGTCAGCAGCTGTATCAGGATCACCAATGGATGCGTGATTTCGGt                                      >  1:1433619/1‑67 (MQ=255)
                  cccGTGGCGTACGCTCTCTGGCCGTCAGCAGCTGTATCAGGATCACCAATGGATGCGTGATTTCGGt                                      >  1:1353817/1‑67 (MQ=255)
                  cccATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGt                                      >  1:1504995/1‑67 (MQ=255)
                  cccATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGt                                      >  1:2768016/1‑67 (MQ=255)
                  cccATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGt                                      >  1:1109549/1‑67 (MQ=255)
                  cccATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGt                                      >  1:1025308/1‑67 (MQ=255)
                  cccATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGt                                      >  1:2622360/1‑67 (MQ=255)
                  cccATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGt                                      >  1:763298/1‑67 (MQ=255)
                  cccATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGt                                      >  1:421434/1‑67 (MQ=255)
                  cccATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGt                                      >  1:2050459/1‑67 (MQ=255)
                       ggCGTACGCTCTCTGGCCGTCAGCAGCTGTATCAGGATCACCAATGGATGCGTGATTTCGGTGAAAGcc                               <  1:1398656/69‑1 (MQ=255)
                       ggCGTACGCTCTCTGGCCGTCAGCAGCTGTATCAGGATCACCAATGGATGCGTGATTTCGGTGAAAGcc                               <  1:2553461/69‑1 (MQ=255)
                       ggCGTACGCTCTCTGGCCGTCAGCAGCTGTATCAGGATCACCAATGGATGCGTGATTTCGGTGAAAGcc                               <  1:1218414/69‑1 (MQ=255)
                       ggCGTACGCTCTCTGGCCGTCAGCAGCTGTATCAGGATCACCAATGGATGCGTGATTTCGGTGAAAGcc                               <  1:1959281/69‑1 (MQ=255)
                       ggCGTACGCTCTCTGGCCGTCAGCAGCTGTATCAGGATCACCAATGGATGCGTGATTTCGGTGAAAGcc                               <  1:727599/69‑1 (MQ=255)
                       ggCGTACGCTCTCTGGCCGTCAGCAGCTGTATCAGGATCACCAATGGATGCGTGATTTCGGTGAAAGcc                               <  1:798643/69‑1 (MQ=255)
                       ggCGTACGCTCTCTGGCCGTCAGCAGCTGTATCAGGATCACCAATGGATGCGTGATTTCGGTGAAAGcc                               <  1:819558/69‑1 (MQ=255)
                       ggCGTACGCTCTCTGGCCGTCAGCAGCTGTATCAGGATCACCAATGGATGCGTGATTTCGGTGAAAGcc                               <  1:860637/69‑1 (MQ=255)
                              gCTCTCTGGTCGTCAGCAACTGTATCAGGATCAGCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTgg                         >  1:531865/1‑68 (MQ=255)
                              gCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCct                              >  1:1215407/1‑63 (MQ=255)
                              gCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTgg                         >  1:1370806/1‑68 (MQ=255)
                                        cgtcAGCAACTGTATCAGGATCACCAGTGGATGCGt                                               <  1:2857752/36‑1 (MQ=255)
                                                      tCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACAcc  <  1:1367227/67‑1 (MQ=255)
                                                      tCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACAcc  <  1:3263614/67‑1 (MQ=255)
                                                      tCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACAcc  <  1:2498311/67‑1 (MQ=255)
                                                        aGGATCATCCGTGGATGCGTGCTTTTGGTGAAAGTCTGGTGGCTTATCGCCCGCCTATCGaca    >  1:577434/1‑63 (MQ=255)
                                                        aGGATCATCCGTGGATGCGTGCTTTTGGTGAAAGTCTGGTGGCTTATCGCCCGCCTATCGaca    >  1:3243257/1‑63 (MQ=255)
                                                        aGGATCATCCGTGGATGCGTGCTTTTGGTGAAAGTCTGGTGGCTTATCGCCCGCCTATCGaca    >  1:889222/1‑63 (MQ=255)
                                                        aGGATCATCCGTGGATGCGTGCTTTTGGTGAAAGTCTGGTGGCTTATCGCCCGCCTATCGaca    >  1:974382/1‑63 (MQ=255)
                                                        aGGATCATCCGTGGATGCGTGCTTTTGGTGAAAGTCTGGTGGCTTATCGCCCGCCTATCGaca    >  1:3121799/1‑63 (MQ=255)
                                                        aGGATCATCCGTGGATGCGTGCTTTTGGTGAAAGTCTGGTGGCTTATCGCCCGCCTATCGaca    >  1:2646295/1‑63 (MQ=255)
                                                        aGGATCATCCGTGGATGCGTGCTTTTGGTGAAAGTCTGGTGGCTTATCGCCCGCCTATCGaca    >  1:2522041/1‑63 (MQ=255)
                                                        aGGATCATCCGTGGATGCGTGCTTTTGGTGAAAGTCTGGTGGCTTATCGCCCGCCTATCGaca    >  1:2498446/1‑63 (MQ=255)
                                                        aGGATCATCCGTGGATGCGTGCTTTTGGTGAAAGTCTGGTGGCTTATCGCCCGCCTATCGaca    >  1:2369506/1‑63 (MQ=255)
                                                        aGGATCATCCGTGGATGCGTGCTTTTGGTGAAAGTCTGGTGGCTTATCGCCCGCCTATCGaca    >  1:2166781/1‑63 (MQ=255)
                                                        aGGATCATCCGTGGATGCGTGCTTTTGGTGAAAGTCTGGTGGCTTATCGCCCGCCTATCGaca    >  1:2100367/1‑63 (MQ=255)
                                                        aGGATCATCCGTGGATGCGTGCTTTTGGTGAAAGTCTGGTGGCTTATCGCCCGCCTATCGaca    >  1:198602/1‑63 (MQ=255)
                                                        aGGATCATCCGTGGATGCGTGCTTTTGGTGAAAGTCTGGTGGCTTATCGCCCGCCTATCGaca    >  1:1917451/1‑63 (MQ=255)
                                                        aGGATCATCCGTGGATGCGTGCTTTTGGTGAAAGTCTGGTGGCTTATCGCCCGCCTATCGaca    >  1:1864697/1‑63 (MQ=255)
                                                        aGGATCATCCGTGGATGCGTGCTTTTGGTGAAAGTCTGGTGGCTTATCGCCCGCCTATCGaca    >  1:1801310/1‑63 (MQ=255)
                                                        aGGATCATCCGTGGATGCGTGCTTTTGGTGAAAGTCTGGTGGCTTATCGCCCGCCTATCGaca    >  1:1787577/1‑63 (MQ=255)
                                                        aGGATCATCCGTGGATGCGTGCTTTTGGTGAAAGTCTGGTGGCTTATCGCCCGCCTATCGaca    >  1:1624262/1‑63 (MQ=255)
                                                        aGGATCATCCGTGGATGCGTGCTTTTGGTGAAAGTCTGGTGGCTTATCGCCCGCCTATCGaca    >  1:1508064/1‑63 (MQ=255)
                                                        aGGATCATCCGTGGATGCGTGCTTTTGGTGAAAGTCTGGTGGCTTATCGCCCGCCTATCGaca    >  1:1114164/1‑63 (MQ=255)
                                                                  |                                                      
CAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACC  >  minE/834626‑834746

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: