| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 836,119 | T→G | 63.5% | A279A (GCT→GCG) | narH → | nitrate reductase 1, beta (Fe‑S) subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 836,119 | 0 | T | G | 63.5% | 33.6 / 27.6 | 41 | A279A (GCT→GCG) | narH | nitrate reductase 1, beta (Fe‑S) subunit |
| Reads supporting (aligned to +/- strand): ref base T (7/8); new base G (6/20); total (13/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.68e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-01 | |||||||||||
CCGTGTGCTCAGAAACCTGTGTCGGTCGTATCCGTTATCTTGGCGTGCTGTTGTACGATGCCGACGCTATTGAACGTGCAGCCAGCACCGAGAACGAGAAAGATCTTTACCAGCGTCAGCTGGACGTGTTCCTC > minE/836052‑836185 | ccGTGTGCTCAGAAACCTGTGTCGGTCGTATCCGTTATCTTGGCGTGCTGTTGTACGATGCCGACGCTa < 1:3390407/69‑1 (MQ=255)ccGTGTGCTCAGAAACCTGTGTCGGTCGTATCCGTTATCTTGGCGTGCTGTTGTACGATGCCGACGCTa < 1:2424121/69‑1 (MQ=255) tgttCAGAAACCTGTGTCGGTCGTATCCGTTATCTTGGCGTGCTGCTGTACGATGCCGACGCGAtt < 1:2871531/63‑1 (MQ=255) tgCTCAGAAACCTGTGTCGGTCGTATCCGTTATCTTGGCGTGCTGTTGTACGATGCCGACGCTATTGaa < 1:1413445/69‑1 (MQ=255) tgCTCAGAAACCTGTGTCGGTCGTATCCGTTATCTTGGCGTGCTGTTGTACGATGCCGACGCTATTGaa < 1:1417569/69‑1 (MQ=255) tgCTCAGAAACCTGTGTCGGTCGTATCCGTTATCTTGGCGTGCTGTTGTACGATGCCGACGCTATTGaa < 1:1438522/69‑1 (MQ=255) tgCTCAGAAACCTGTGTCGGTCGTATCCGTTATCTTGGCGTGCTGTTGTACGATGCCGACGCTATTGaa < 1:3364354/69‑1 (MQ=255) tCAGAAACCTGTGTCGGTCGTATCCGTTATCTTGGCGTGCTGTTGTACGATGCCGACGCTATTGaa < 1:1148584/66‑1 (MQ=255) tcggtcgTATCCGTTATCTTGGCGTGCTGCTGTACGATGCCGACGCGATTGAACGTGcggcaagta > 1:1500408/1‑64 (MQ=255) tcggtcgTATCCGTTATCTTGGCGTGCTGCTGTACGATGCCGACGCGATTGAACGTGcggcaagta > 1:2654348/1‑64 (MQ=255) tcggtcgTATCCGTTATCTTGGCGTGCTGCTGTACGATGCCGACGCGATTGAACGTGcggcaagta > 1:243255/1‑64 (MQ=255) tcggtcgTATCCGTTATCTTGGCGTGCTGCTGTACGATGCCGACGCGATTGAACGTGcggcaagta > 1:1218498/1‑64 (MQ=255) tcggtcgTATCCGTTATCTTGGCGTGCTGCTGTACGATGCCGACGCGATTGAACGTGcggcaagta > 1:1151431/1‑64 (MQ=255) tcggtcgTATCCGTTATCTTGGCGTGCTGCTGTACGATGCCGACGCGATTGAACGTGcggcaagta > 1:3183048/1‑64 (MQ=255) cGTTATCTTGGCGTGCTGTTGTACGATGCCGACGCTATTGAACGTGCAGCCAGCACcgagaacga > 1:778388/1‑65 (MQ=255) aTCTTGGCGTGCTGTTGTACGATGCCGACGCTATTGAACGTg > 1:416477/1‑42 (MQ=255) ttGGCGTGCTGTTGTACGATGCCGACGCTATTGAACGTGCAGCCAGCACCGAGAACGAGAAAGATCtt < 1:925299/68‑1 (MQ=255) gctgtACGATGCCGACGCGATTGAACGTGCGGCAAGTACTGAGAACGAGAAAGATCTCTACCAGCGTCa < 1:238120/67‑1 (MQ=255) gctgtACGATGCCGACGCGATTGAACGTGCGGCAAGTACCGAGAACGAGAAAGATCTCTACCAGCGTCa < 1:3351754/67‑1 (MQ=255) gctgtACGATGCCGACGCGATTGAACGTGCGGCAAGTACCGAGAACGAGAAAGATCTCTACCAGCGTCa < 1:546074/67‑1 (MQ=255) gctgtACGATGCCGACGCGATTGAACGTGCGGCAAGTACCGAGAACGAGAAAGATCTCTACCAGCGTCa < 1:344941/67‑1 (MQ=255) gctgtACGATGCCGACGCGATTGAACGTGCGGCAAGTACCGAGAACGAGAAAGATCTCTACCAGCGTCa < 1:522549/67‑1 (MQ=255) gctgtACGATGCCGACGCGATTGAACGTGCGGCAAGTACCGAGAACGAGAAAGATCTCTACCAGCGTCa < 1:2791760/67‑1 (MQ=255) gctgtACGATGCCGACGCGATTGAACGTGCGGCAAGTACCGAGAACGAGAAAGATCTCTACCAGCGTCa < 1:650158/67‑1 (MQ=255) gctgtACGATGCCGACGCGATTGAACGTGCGGCAAGTACCGAGAACGAGAAAGATCTCTACCAGCGTCa < 1:989059/67‑1 (MQ=255) gctgtACGATGCCGACGCGATTGAACGTGCGGCAAGTACCGAGAACGAGAAAGATCTCTACCAGCGTCa < 1:3302751/67‑1 (MQ=255) gctgtACGATGCCGACGCGATTGAACGTGCGGCAAGTACCGAGAACGAGAAAGATCTCTACCAGCGTCa < 1:1055057/67‑1 (MQ=255) gctgtACGATGCCGACGCGATTGAACGTGCGGCAAGTACCGAGAACGAGAAAGATCTCTACCAGCGTCa < 1:2465319/67‑1 (MQ=255) gctgtACGATGCCGACGCGATTGAACGTGCGGCAAGTACCGAGAACGAGAAAGATCTCTACCAGCGTCa < 1:1847760/67‑1 (MQ=255) gctgtACGATGCCGACGCGATTGAACGTGCGGCAAGTACCGAGAACGAGAAAGATCTCTACCAGCGTCa < 1:16617/67‑1 (MQ=255) gctgtACGATGCCGACGCGATTGAACGTGCGGCAAGTACCGAGAACGAGAAAGATCTCTACCAGCGTCa < 1:1483394/67‑1 (MQ=255) gctgtACGATGCCGACGCGATTGAACGTGCGGCAAGTACCGAGAACGAGAAAGATCTCTACCAGCGTCa < 1:1380268/67‑1 (MQ=255) gctgtACGATGCCGACGCGATTGAACGTGCGGCAAGTACCGAGAACGAGAAAGATCTCTACCAGCGTCa < 1:1081539/67‑1 (MQ=255) gctgtACGATGCCGACGCGATTGAACGTGCGGCAAGTACCGAGAACGAGAAAGAGCTCTACCAGCGTCa < 1:471790/67‑1 (MQ=255) ctgtACGATGCCGACGCGATTGAACGTGCGGCAAGTACCGAGAACGAGAAAGATCTCTACCAGCGTCa < 1:2974790/67‑1 (MQ=255) ctgtACGATGCCGACGCGATTGAACGTGCGGCAAGTACCGAGAACGAGAAAGATCTCTACCAGCGTCa < 1:3382707/67‑1 (MQ=255) aTGCCGACGCTATTGAACGTGCAGCCAGCACCGAGAACGAGAAAGATCTTTACCAGCGTCAGCTGGACg > 1:2892679/1‑69 (MQ=255) aTGCCGACGCTATTGAACGTGCAGCCAGCACCGAGAACGAGAAAGATCTTTACCAGCGTCAGCTGGACg > 1:2343760/1‑69 (MQ=255) aTGCCGACGCTATTGAACGTGCAGCCAGCACCGAGAACGAGAAAGATCTTTACCAGCGTCAGCTGGACg > 1:557210/1‑69 (MQ=255) aTGCCGAAGCTATTGAACGTGCAGCCAGCACCGAGAACGAGAAAGATCTTTACCAGCGTCAGCTGGACg > 1:1729600/1‑69 (MQ=255) gCTATTGAACGTGCAGCCAGCACCGAGAACGAGAAAGATCTTTACCAGCGTCAGCTGGACGTGTTCCTc > 1:3386408/1‑69 (MQ=255) | CCGTGTGCTCAGAAACCTGTGTCGGTCGTATCCGTTATCTTGGCGTGCTGTTGTACGATGCCGACGCTATTGAACGTGCAGCCAGCACCGAGAACGAGAAAGATCTTTACCAGCGTCAGCTGGACGTGTTCCTC > minE/836052‑836185 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |