| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 839,437 | G→C | 100% | intergenic (‑136/+24) | tyrT ← / ← purU | tRNA‑Tyr/formyltetrahydrofolate hydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 839,437 | 0 | G | C | 87.5% | 48.1 / 0.5 | 24 | intergenic (‑136/+24) | tyrT/purU | tRNA‑Tyr/formyltetrahydrofolate hydrolase |
| Reads supporting (aligned to +/- strand): ref base G (2/1); new base C (0/21); total (2/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.09e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGT > minE/839388‑839503 | ttGAGAAAAATGAACTGGTTGCGTGATTTTCATCCGTAACGGATTAAAGCTAACCAGTTATTTTTGCTg < 1:2968445/69‑1 (MQ=255)ttGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGCTAACCAGTTATTTTTGCTg < 1:246217/69‑1 (MQ=255)ttGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGCTAACCAGTTATTTTTGCTg < 1:939450/69‑1 (MQ=255)ttGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGCTAACCAGTTATTTTTGCTg < 1:769543/69‑1 (MQ=255)ttGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGCTAACCAGTTATTTTTGCTg < 1:529670/69‑1 (MQ=255)ttGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGCTAACCAGTTATTTTTGCTg < 1:398825/69‑1 (MQ=255)ttGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGCTAACCAGTTATTTTTGCTg < 1:364933/69‑1 (MQ=255)ttGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGCTAACCAGTTATTTTTGCTg < 1:3418813/69‑1 (MQ=255)ttGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGCTAACCAGTTATTTTTGCTg < 1:3405356/69‑1 (MQ=255)ttGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGCTAACCAGTTATTTTTGCTg < 1:2617838/69‑1 (MQ=255)ttGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGCTAACCAGTTATTTTTGCTg < 1:1044671/69‑1 (MQ=255)ttGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGCTAACCAGTTATTTTTGCTg < 1:2358755/69‑1 (MQ=255)ttGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGCTAACCAGTTATTTTTGCTg < 1:2342001/69‑1 (MQ=255)ttGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGCTAACCAGTTATTTTTGCTg < 1:2327097/69‑1 (MQ=255)ttGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGCTAACCAGTTATTTTTGCTg < 1:1967201/69‑1 (MQ=255)ttGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGCTAACCAGTTATTTTTGCTg < 1:1887615/69‑1 (MQ=255)ttGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGCTAACCAGTTATTTTTGCTg < 1:1843769/69‑1 (MQ=255)ttGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGCTAACCAGTTATTTTTGCTg < 1:1779070/69‑1 (MQ=255)ttGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGCTAACCAGTTATTTTTCCTg < 1:910477/69‑1 (MQ=255) tAATTTTCATCCGTAACGGATTAAAGCTAACCAGTTATTTTTGCTg < 1:1795057/46‑1 (MQ=255) aaTTTTCATCCGTAACGGATTAAAGCTAACCAGTTATTTTTGCTg < 1:2327098/45‑1 (MQ=255) ttCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTAc > 1:371480/1‑68 (MQ=255) ttCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTAc > 1:1482602/1‑68 (MQ=255) tAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACa < 1:343937/68‑1 (MQ=255) ggTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCtgt > 1:2399458/1‑68 (MQ=255) ggTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCtgt > 1:1758159/1‑68 (MQ=255) | TTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGT > minE/839388‑839503 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |