| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 842,247 | A→G | 27.9% | P109P (CCA→CCG) | rssB → | response regulator of RpoS |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 842,247 | 0 | A | G | 27.9% | 76.2 / 31.0 | 75 | P109P (CCA→CCG) | rssB | response regulator of RpoS |
| Reads supporting (aligned to +/- strand): ref base A (28/26); new base G (2/19); total (30/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.45e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01 | |||||||||||
GAAAATATGGCAGATATTGCCAAAGCGTTACGTCTGGGCGTTGAAGATGTTTTGCTGAAACCAGTTAAAGATCTGAATCGCTTGCGCGAGATGGTTTTTGCCTGTCTCTATCCCAGCATGTTTAA > minE/842185‑842309 | gAAAATATGGCAGATATTGCCAAAGCGTTACGTCTGGGCGTTGAAGATGTTTTGCTGAAACCAg > 1:2603445/1‑64 (MQ=255)gAAAATATGGCAGATATTGCCAAAGCGTTACGTCTGGGCGTTGAAGATGTTTTGCTGAAACCAGt > 1:2764632/1‑65 (MQ=255)gAAAATATGGCAGATATTGCCAAAGCGTTACGTCTGGGCGTTGAAGATGTTTTGCTGAAACCAGt > 1:2681343/1‑65 (MQ=255)gAAAATATGGCAGATATTGCCAAAGCGTTACGTCTGGGCGTTGAAGATGTTTTGCTGAAACCAGt > 1:1300889/1‑65 (MQ=255)gAAAATATGGCAGATATTGCCAAAGCGTTACGTCTGGGCGTTGAAGATGTTTTGCTGAAACCAGt > 1:2434588/1‑65 (MQ=255)gAAAATATGGCAGATATTGCCAAAGCGTTACGTCTGGGCGTTGAAGATGTTTTGCTGAAACCAGt > 1:1373085/1‑65 (MQ=255)gAAAATATGGCAGATATTGCCAAAGCGTTACGTCTGGGCGTTGAAGATGTTTTGCTGAAACCAGt > 1:3280352/1‑65 (MQ=255)gAAAATATGGCAGATATTGCCAAAGCGTTACGTCTGGGCGTTGAAGATGTTTTGCTGAAACCAGt > 1:1943196/1‑65 (MQ=255)gAAAATATGGCAGATATTGCCAAAGCGTTACGTCTGGGCGTTGAAGATGTTTTGCTGAAACCAGt > 1:345369/1‑65 (MQ=255)gAAAATATGGCAGATATTGCCAAAGCGTTACGTCTGGGCGTTGAAGATGTTTTGCTGAAACCAGt > 1:582683/1‑65 (MQ=255) aaTATGGCAGATATTGCCAAAGCGTTACGTCTGGGCGTTGAAGATGTTTTGCTGAAACCAGTTAAAg < 1:981020/67‑1 (MQ=255) aaTATGGCAGATATTGCCAAAGCGTTACGTCTGGGCGTTGAAGATGTTTTGCTGAAACCAGTTAAAg < 1:3056463/67‑1 (MQ=255) aaTATGGCAGATATTGCCAAAGCGTTACGTCTGGGCGTTGAAGATGTTTTGCTGAAACCAGTTAAAg < 1:2605483/67‑1 (MQ=255) aaTATGGCAGATATTGCCAAAGCGTTACGTCTGGGCGTTGAAGATGTTTTGCTGAAACCAGTTAAAg < 1:2132268/67‑1 (MQ=255) aaTATGGCAGATATTGCCAAAGCGTTACGTCTGGGCGTTGAAGATGTTTTGCTGAAACCAGTTAAAg < 1:349066/67‑1 (MQ=255) aaTATGGCAGATATTGCCAAAGCGTTACGTCTGGGCGTTGAAGATGTTTTGCTGAAACCAGTTAAAg < 1:1582528/67‑1 (MQ=255) tatGGCAGATATTGCCAAAGCGTTACGTCTGGGTGTTGAAGATGTTTTGCTGAAACAGGTTAAAGATCt < 1:538269/69‑1 (MQ=255) tatGGCAGATATTGCCAAAGCGTTACGTCTGGGTGTTGAAGATGTTTTGCTGAAACAGGTTAAAGATCt < 1:301774/69‑1 (MQ=255) tatGGCAGATATTGCCAAAGCGTTACGTCTGGGTGTTGAAGATGTTTTGCTGAAACAGGTTAAAGATCt < 1:2810511/69‑1 (MQ=255) tatGGCAGATATTGCCAAAGCGTTACGTCTGGGTGTTGAAGATGTTTTGCTGAAACAGGTTAAAGATCt < 1:1094162/69‑1 (MQ=255) tatGGCAGATATTGCCAAAGCGTTACGTCTGGGTGTTGAAGATGTTTTGCTGAAACAGGTTAAAGATCt < 1:2734064/69‑1 (MQ=255) tatGGCAGATATTGCCAAAGCGTTACGTCTGGGTGTTGAAGATGTTTTGCTGAAACAGGTTAAAGATCt < 1:1011407/69‑1 (MQ=255) tatGGCAGATATTGCCAAAGCGTTACGTCTGGGTGTTGAAGATGTTTTGCTGAAACAGGTTAAAGATCt < 1:973122/69‑1 (MQ=255) tatGGCAGATATTGCCAAAGCGTTACGTCTGGGTGTTGAAGATGTTTTGCTGAAACAGGTTAAAGATCt < 1:3095635/69‑1 (MQ=255) tatGGCAGATATTGCCAAAGCGTTACGTCTGGGTGTTGAAGATGTTTTGCTGAAACAGGTTAAAGATCt < 1:2576148/69‑1 (MQ=255) tatGGCAGATATTGCCAAAGCGTTACGTCTGGGTGTTGAAGATGTTTTGCTGAAACAGGTTAAAGATCt < 1:3118346/69‑1 (MQ=255) tatGGCAGATATTGCCAAAGCGTTACGTCTGGGTGTTGAAGATGTTTTGCTGAAACAGGTTAAAGATCt < 1:1329933/69‑1 (MQ=255) tatGGCAGATATTGCCAAAGCGTTACGTCTGGGTGTTGAAGATGTTTTGCTGAAACAGGTTAAAGATCt < 1:2043232/69‑1 (MQ=255) tatGGCAGATATTGCCAAAGCGTTACGTCTGGGTGTTGAAGATGTTTTGCTGAAACAGGTTAAAGATCt < 1:3387651/69‑1 (MQ=255) tatGGCAGATATTGCCAAAGCGTTACGTCTGGGTGTTGAAGATGTTTTGCTGAAACAGGTTAAAGATCt < 1:3427965/69‑1 (MQ=255) tatGGCAGATATTGCCAAAGCGTTACGTCTGGGTGTTGAAGATGTTTTGCTGAAACAGGTTAAAGATCt < 1:1759341/69‑1 (MQ=255) atGGCAGATATTGCCAAAGCGTTACGTCTGGGTGTTGAAGATGTTTTGCTGAAACAGGTTAAAGATCt < 1:3280661/68‑1 (MQ=255) atGGCAGATATTGCCAAAGCGTTACGTCTGGGTGTTGAAGATGTTTTGCTGAAACAGGTTAAAGATCt < 1:1658326/68‑1 (MQ=255) atGGCAGATATTGCCAAAGCGTTACGTCTGGGTGTTGAAGATGTTTTGCTGAAACAGGTTAAAGATCt < 1:1576959/68‑1 (MQ=255) atGGCAGATATTGCCAAAGCGTTACGTCTGGGTGTTGAAGATGTTTTGCTGAAACAGGTTAAAGATCt < 1:512019/68‑1 (MQ=255) tatTGCCAAAGCGTTACGTCTGGGCGTTGAAGATGTTTTGCTGAAACCAGTTAAAGATCTGAATCGCtt > 1:1390638/1‑69 (MQ=255) tatTGCCAAAGCGTTACGTCTGGGCGTTGAAGATGTTTTGCTGAAACCAGTTAAAGATCTGAATCGCtt > 1:1451356/1‑69 (MQ=255) ccAAAGCGTTACGTCTGGGCGTTGAAGATGTTTTGCTGAAACCAGTTAAAg < 1:2859894/51‑1 (MQ=255) aaaGCGTTACGTCTGGGCGTTGAAGATGTTTTGCTGAAACCAGTTAAAGATCTGAATCGCTTGCGCga < 1:2185119/68‑1 (MQ=255) aaaGCGTTACGTCTGGGCGTTGAAGATGTTTTGCTGAAACCAGTTAAAGATCTGAATCGCTTGCGCga < 1:898052/68‑1 (MQ=255) tCTGGGCGTTGAAGATGTTTTGCTGAAACCAGTTAAAGATCTGAATCGCTTGCGCGAGATGGTTTTTg < 1:2955552/68‑1 (MQ=255) tCTGGGCGTTGAAGATGTTTTGCTGAAACCAGTTAAAGATCTGAATCGCTTGCGCGAGATGGTTTTTg < 1:2690678/68‑1 (MQ=255) tCTGGGCGTTGAAGATGTTTTGCTGAAACCAGTTAAAGATCTGAATCGCTTGCGCGAGATGGTTTTTg < 1:690604/68‑1 (MQ=255) tCTGGGCGTTGAAGATGTTTTGCTGAAACCAGTTAAAGATCTGAATCGCTTGCGCGAGATGGTTTTTg < 1:710652/68‑1 (MQ=255) tCTGGGCGTTGAAGATGTTTTGCTGAAACCAGTTAAAGATCTGAATCGCTTGCGCGAGATGGTTTTTg < 1:3217295/68‑1 (MQ=255) gATGTTTTGCTGAAACCAGTTAAAGATCTGAATCGCTTGCGCGAGATGGTTTTTGCCTGTCTCTATccc > 1:1730674/1‑69 (MQ=255) gATGTTTTGCTGAAACCAGTTAAAGATCTGAATCGCTTGCGCGAGATGGTTTTTGCCTGTCTCTATccc > 1:1734533/1‑69 (MQ=255) gATGTTTTGCTGAAACCAGTTAAAGATCTGAATCGCTTGCGCGAGATGGTTTTTGCCTGTCTCTATccc > 1:1853037/1‑69 (MQ=255) tGTTTTGCTGAAACCAGTTAAAGATCTGAATCGCTTGTGCTAGATGGTTTTTGCCTGTCTCTATCCCAg > 1:2750095/1‑69 (MQ=255) tGTTTTGCTGAAACCAGTTAAAGATCTGAATCGCTTGCGCGAGATGGTTTTTGCCTGtctc < 1:1044391/61‑1 (MQ=255) tGTTTTGCTGAAACCAGTTAAAGATCTGAATCGCTTGCGCGAGATGGTTTTTGCCTGtctc < 1:165524/61‑1 (MQ=255) tGTTTTGCTGAAACCAGTTAAAGATCTGAATCGCTTGCGCGAGATGGTTTTTGCCTGTCTCTATCCCAg < 1:2901585/69‑1 (MQ=255) tGTTTTGCTGAAACCAGTTAAAGATCTGAATCGCTTGCGCGAGATGGTTTTTGCCTGTCTCTATCCCAg > 1:966129/1‑69 (MQ=255) tGTTTTGCTGAAACCAGTTAAAGATCTGAATCGCTTGCGCGAGATGGTTTTTGCCTGTCTCTATCCCAg > 1:1254708/1‑69 (MQ=255) tGTTTTGCTGAAACCAGTTAAAGATCTGAATCGCTTGCGCGAGATGGTTTTTGCCTGTCTCTATCCCAg > 1:827847/1‑69 (MQ=255) tGTTTTGCTGAAACCAGTTAAAGATCTGAATCGCTTGCGCGAGATGGTTTTTGCCTGTCTCTATCCCAg < 1:1955829/69‑1 (MQ=255) tGTTTTGCTGAAACCAGTTAAAGATCTGAATCGCTTGCGCGAGATGGTTTTTGCCTGTCTCTATCCCAg < 1:1833094/69‑1 (MQ=255) tGTTTTGCTGAAACCAGTTAAAGATCTGAATCGCTTGCGCGAGATGGTTTTTGCCTGTCTCTATCCCAg < 1:391266/69‑1 (MQ=255) tGTTTTGCTGAAACCAGTTAAAGATCTGAATCGCTTGCGCGAGATGGTTTTTGCCTGTCTCTATCCCAg < 1:1693874/69‑1 (MQ=255) tGTTTTGCTGAAACCAGTTAAAGATCTGAATCGCTTGCGCGAGATGGTTTTTGCCTGTCTCTATCACAg < 1:1485652/69‑1 (MQ=255) tGTTTTGCTGAAACCAGTTAAAGATCTGAATCGCTTGCACGAGATGGTTTTTGCCTGTCTCTATCCCAg < 1:3432003/69‑1 (MQ=255) gTTTTGCTGAAACCAGTTAAAGATCTGAATCGCTTGCGCGAGATGGtt < 1:225736/48‑1 (MQ=255) gTTTTGCTGAAACCAGTTAAAGATCTGAATCGCTTGCGCGAGATGGtt < 1:131603/48‑1 (MQ=255) gTTTTGCTGAAACCAGTTAAAGATCTGAATCGCTTGCGCGAGATGGTTTTTGCCTGTCTCTATCCCAGc > 1:463796/1‑69 (MQ=255) gTTTTGCTGAAACCAGTTAAAGATCTGAATCGCTTGCGCGAGATGGTTTTTGCCTGTCTCTATCCCAGc > 1:453733/1‑69 (MQ=255) gTTTTGCTGAAACCAGTTAAAGATCTGAATCGCTTGCGCGAGATGGTTTTTGCCTGTCTCTATCCCAGc > 1:2881308/1‑69 (MQ=255) gTTTTGCTGAAACCAGTTAAAGATCTGAATCGCTTGCGCGAGATGGTTTTTGCCTGTCTCTATCCCAGc > 1:2589226/1‑69 (MQ=255) gTTTTGCTGAAACCAGTTAAAGATCTGAATCGCTTGCGCGAGATGGTTTTTGCCTGTCTCTATCCCAGc > 1:701274/1‑69 (MQ=255) gTTTTGCTGAAACCAGTTAAAGATCTGAATCGCTTGCGCGAGATGGTTTTTGCCTGTCTCTATCCCAGc > 1:2207180/1‑69 (MQ=255) gTTTTGCTGAAACCAGTTAAAGATCTGAATCGCTTGCGCGAGATGGTTTTTGCCTGTCTCTATCCCAGc > 1:1186764/1‑69 (MQ=255) gTTTTGCTGAAACCAGTTAAAGATCTGAATCGCTTGCGCGAGATGGTTTTTGCCTGTCTCTATCCCAGc > 1:3134436/1‑69 (MQ=255) ttGCTGAAACCGGTTAAAGATCTGAATCGCTTGCGCGAGATGGTTTTTGCCTGTCTCTATCCCAGCATg > 1:2564578/1‑69 (MQ=255) ttGCTGAAACCGGTTAAAGATCTGAATCGCTTGCGCGAGATGGTTTTTGCCTGTCTCTATCCCAGCATg > 1:2065715/1‑69 (MQ=255) ttGCTGAAACCAGTTAAAGATCTGAATCGCTTGCGCGAGATGGTTTTTGCCTGTCTCTATCCCAGCATg > 1:304426/1‑69 (MQ=255) gAAACCAGTTAAAGATCCGAATCGCTTGCGCGAGATGGTTTTTGCCTGTCTCTATCCCAGCATGTTTaa < 1:3363705/69‑1 (MQ=255) | GAAAATATGGCAGATATTGCCAAAGCGTTACGTCTGGGCGTTGAAGATGTTTTGCTGAAACCAGTTAAAGATCTGAATCGCTTGCGCGAGATGGTTTTTGCCTGTCTCTATCCCAGCATGTTTAA > minE/842185‑842309 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |