| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 843,576 | G→A | 41.4% | L147L (TTG→TTA) | galU → | glucose‑1‑phosphate uridylyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 843,576 | 0 | G | A | 41.4% | 43.4 / 75.8 | 106 | L147L (TTG→TTA) | galU | glucose‑1‑phosphate uridylyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (31/31); new base A (28/16); total (59/47) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.73e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.41e-01 | |||||||||||
Only 100 of 106 total aligned reads displayed.
TAGTGGGTGATGAACCGGTAGCTGTTATTTTGCCTGATGTTATTCTGGATGAATATGAATCCGATTTGTCACAGGATAACCTGGCAGAGATGATCCGCCGCTTTGATGAAACGGGTCATAGCCAGATCAT > minE/843509‑843638 | tAGTGGGTGATGAACCGGTAGCTGTTATTTTGCCTGATGTTATTCTGGATGAATATGAATCCGATTTGt > 1:852489/1‑69 (MQ=255)tAGTGGGTGATGAACCGGTAGCTGTTATTTTGCCTGATGTTATTCTGGATGAATATGAATCCGATTTGt > 1:3148640/1‑69 (MQ=255)tAGTGGGTGATGAACCGGTAGCTGTTATTTTGCCTGATGTTATTCTGGATGAATATGAATCCGATTTGt > 1:1837288/1‑69 (MQ=255)tAGTGGGTGATGAACCGGTAGCTGTTATTTTGCCTGATGTTATTCTGGATGAATATGAATCCGATTTGt > 1:2309615/1‑69 (MQ=255) ggTGATGAACCGGTAGCTGTTATTTTGCCTGATGTTATTCTGGATGAATATGAATCCGATTTGTCACAg > 1:2043149/1‑69 (MQ=255) ggTGATGAACCGGTAGCTGTTATTTTGCCTGATGTTATTCTGGATGAATATGAATCCGATTTGTCACAg > 1:424521/1‑69 (MQ=255) atgaACCGGTAGCTGTTATTTTGCCTGATGTTATTCTGGATGAATATGAATCCGATTTGTCACAGGATa < 1:211833/69‑1 (MQ=255) atgaACCGGTAGCTGTTATTTTGCCTGATGTTATTCTGGATGAATATGAATCCGATTTGTCACAGGATa < 1:2076643/69‑1 (MQ=255) atgaACCGGTAGCTGTTATTTTGCCTGATGTTATTCTGGATGAATATGAATCCGATTTGTCACAGGATa < 1:3419877/69‑1 (MQ=255) atgaACCGGTAGCTGTTATTTTGCCTGATGTTATTCTGGATGAATATGAATCCGATTTGTCACAGGATa < 1:866821/69‑1 (MQ=255) atgaACCGGTAGCTGTTATTTTGCCTGATGTTATTCTGGATGAATATGAATCCGATTTGTCACAGGATa < 1:839200/69‑1 (MQ=255) atgaACCGGTAGCTGTTATTTTGCCTGATGTTATTCTGGATGAATATGAATCCGATTTGTCACAGGATa < 1:2715308/69‑1 (MQ=255) tgaACCGGTAGCTGTTATTTTGCCTGATGTTATTCTGGATGAATATGAATCCGATTTGTCACAGGATa < 1:3283317/68‑1 (MQ=255) gaACCGGTAGCTGTTATTTTGCCTGATGTTATTCTGGATGAATATGAATCCGATTTGTCACAGGATaa < 1:3360500/68‑1 (MQ=255) gaACCGGTAGCTGTTATTTTGCCTGATGTTATTCTGGATGAATATGAATCCGATTTGTCACAGGATaa < 1:437408/68‑1 (MQ=255) gaACCGGTAGCTGTTATTTTGCCTGATGTTATTCTGGATGAATATGAATCCGATTTGTCACAGGATaa < 1:375218/68‑1 (MQ=255) gaACCGGTAGCTGTTATTTTGCCTGATGTTATTCTGGATGAATATGAATCCGATTTGTCACAGGATaa < 1:620776/68‑1 (MQ=255) aaCCGGTAGCTGTTATTTTGCCTGATGTTATTCTGGATGAATATGAATCCGATTTGTCACAGGATaa < 1:3425126/67‑1 (MQ=255) gCTGTTATTTTGCCTGATGTTATTCTGGATGAATATGAATCCGATTTGTCACAGGATAACCTGGCagag > 1:563691/1‑69 (MQ=255) gCTGTTATTTTGCCTGATGTTATTCTGGATGAATATGAATCCGATTTGTCACAGGATAACCTGGCagag > 1:2113858/1‑69 (MQ=255) gTTATTTTGCCTGATGTTATTCTGGATGAATATGAATCCGATTTGTCACAGGATAACCTGGCAGAgatg > 1:1134399/1‑69 (MQ=255) gTTATTTTGCCTGACGTTATTCTGGATGAATATGAATCCGATTTGTCACAGGATAACCTGGCAGAgatg > 1:261417/1‑69 (MQ=255) gTTATTTTGCCTGACGTTATTCTGGATGAATATGAATCCGATTTATc > 1:1430798/1‑47 (MQ=255) gTTATTTTGCCTGACGTTATTCTGGATGAATATGAATCCGATTTATCACAGGATaa > 1:1919693/1‑56 (MQ=255) gTTATTTTGCCTGACGTTATTCTGGATGAATATGAATCCGATTTATCACAGGATAACCTGGCAGAgatg > 1:2698015/1‑69 (MQ=255) gTTATTTTGCCTGACGTTATTCTGGATGAATATGAATCCGATTTATCACAGGATAACCTGGCAGAgatg > 1:2596275/1‑69 (MQ=255) gTTATTTTGCCTGACGTTATTCTGGATGAATATGAATCCGATTTATCACAGGATAACCTGGCAGAgatg > 1:2783329/1‑69 (MQ=255) gTTATTTTGCCTGACGTTATTCTGGATGAATATGAATCCGATTTATCACAGGATAACCTGGCAGAgatg > 1:3035063/1‑69 (MQ=255) gTTATTTTGCCTGACGTTATTCTGGATGAATATGAATCCGATTTATCACAGGATAACCTGGCAGAgatg > 1:2252101/1‑69 (MQ=255) gTTATTTTGCCTGACGTTATTCTGGATGAATATGAATCCGATTTATCACAGGATAACCTGGCAGAgatg > 1:3319015/1‑69 (MQ=255) gTTATTTTGCCTGACGTTATTCTGGATGAATATGAATCCGATTTATCACAGGATAACCTGGCAGAgatg > 1:2183235/1‑69 (MQ=255) gTTATTTTGCCTGACGTTATTCTGGATGAATATGAATCCGATTTATCACAGGATAACCTGGCAGAgatg > 1:1106835/1‑69 (MQ=255) gTTATTTTGCCTGACGTTATTCTGGATGAATATGAATCCGATTTATCACAGGATAACCTGGCAGAgatg > 1:2169359/1‑69 (MQ=255) gTTATTTTGCCTGACGTTATTCTGGATGAATATGAATCCGATTTATCACAGGATAACCTGGCAGAgatg > 1:106960/1‑69 (MQ=255) gTTATTTTGCCTGACGTTATTCTGGATGAATATGAATCCGATTTATCACAGGATAACCTGGCAGAgatg > 1:1710724/1‑69 (MQ=255) gTTATTTTGCCTGACGTTATTCTGGATGAATATGAATCCGATTTATCACAGGATAACCTGGCAGAgatg > 1:1389171/1‑69 (MQ=255) gTTATTTTGCCTGACGTTATTCTGGATGAATATGAATCCGATTTATCACAGGATAACCTGGCAGAgatg > 1:1520856/1‑69 (MQ=255) gTTATTTTGCCTGACGTTATTCTGGATGAATATGAATCCGATTTATCACAGGATAACCTGGCAGAgatg > 1:162958/1‑69 (MQ=255) gTTATTTTGCCTGACGTTATTCTGGATGAATATGAATCCGATTTATCACAGGATAACATGGCAGAgatg > 1:2677236/1‑69 (MQ=255) aTTTTGCCTGATGTTATTCTGGATGAATATGAATCCGATTTGTCACAGGATAACCTGGCAGAgatga < 1:2822806/67‑1 (MQ=255) gTTATTCTGTATGAATATGAATCCGATTTATCACAGGATAACCTGGCAGAGATGATCCGGCGCTTtgat < 1:459797/69‑1 (MQ=255) gTTATTCTGGATTAATATGAATCCGATTTATCACAGGATAACCTGGCAGAGATGATCGGCCGCTTtgat < 1:3407745/69‑1 (MQ=255) gTTATTCTGGATGAATATGAATCCGATTTGTCACAGGATAACCTGGCaga > 1:1331381/1‑50 (MQ=255) gTTATTCTGGATGAATATGAATCCGATTTGTCACAGGATAACCTGGCaga > 1:2570050/1‑50 (MQ=255) gTTATTCTGGATGAATATGAATCCGATTTGTCACAGGATAACCTGGCaga > 1:728784/1‑50 (MQ=255) gTTATTCTGGATGAATATGAATCCGATTTGTAACAGGATAACCTGGCaga > 1:749426/1‑50 (MQ=255) gTTATTCTGGATGAATATGAATCCGATTTATCACATGATAACCTGGCAGAGATGATCCGCCGCTTtgat < 1:59417/69‑1 (MQ=255) gTTATTCTGGATGAATATGAATCCGATTTATCACAGGATAACCTGGCAGAGATGATCCGCCGCTTtgat < 1:2104415/69‑1 (MQ=255) gTTATTCTGGATGAATATGAATCCGATTTATCACAGGATAACCTGGCAGAGATGATCCGCCGCTTtgat < 1:3230498/69‑1 (MQ=255) gTTATTCTGGATGAATATGAATCCGATTTATCACAGGATAACCTGGCAGAGATGATCCGCCGCTTtgat < 1:285050/69‑1 (MQ=255) gTTATTCTGGATGAATATGAATCCGATTTATCACAGGATAACCTGGCAGAGATGATCCGCCGCTTtgat < 1:3302125/69‑1 (MQ=255) gTTATTCTGGATGAATATGAATCCGATTTATCACAGGATAACCTGGCAGAGATGATCCGCCGCTTtgat < 1:1773909/69‑1 (MQ=255) gTTATTCTGGATGAATATGAATCCGATTTATCACAGGATAACCTGGCAGAGATGATCCGCCGCTTtgat < 1:1138445/69‑1 (MQ=255) gTTATTCTGGATGAATATGAATCCGATTTATCACAGGATAACCTGGCAGAGATGATCCGCCGCTTtgat < 1:2421030/69‑1 (MQ=255) gTTATTCTGGATGAATATGAATCCGATTTATCACAGGATAACCTGGCAGAGATGATCCGCCGCTTtgat < 1:355468/69‑1 (MQ=255) gTTATTCTGGATGAATATGAATCCGATTTATCACAGGATAACCTGGCAGAGATGATCCGCCGCTTtgat < 1:1629913/69‑1 (MQ=255) gTTATTCTGGATGAATATGAATCCGATTTATCACAGGATAACCTGGCAGAGATGATCCGCCGCTTtgat < 1:3413296/69‑1 (MQ=255) ttATTCTGGATGAATATGAATCCGATTTATCACAGGATAACCTGGCAGAGATGATCCGCCGCTTtgat < 1:356973/68‑1 (MQ=255) tATTCTGGATGAATATGAATCCGATTTGTCACAGGATAACCTGGCAGAGATGATCCGCCGCTTtgatga > 1:2449596/1‑69 (MQ=255) aTTCTGGATGTATATGAATCCGATTTATCACAGGATAACCTGGCAGAGATGATCCGCCGCTTtgat < 1:3299957/66‑1 (MQ=255) aTTCTGGATGAATATGAATCCGATTTGTCACAGGATAACCTGGCAGAGATGATCCGCCGCTTtgatga < 1:2431758/68‑1 (MQ=255) aTTCTGGATGAATATGAATCCGATTTGTCACAGGATAACCTGGCAGAGATGATCCGCCGCTTtgatga < 1:746695/68‑1 (MQ=255) aTTCTGGATGAATATGAATCCGATTTGTCACAGGATAACCTGGCAGAGATGATCCGCCGCTTtgatga < 1:2993425/68‑1 (MQ=255) cTGGATGAATATGAATCCGATTTGTCACAGGATAACCTGGCAGAGATGATCCGCCGCTTTGATGaaa > 1:2357317/1‑67 (MQ=255) cTGGATGAATATGAATCCGATTTGTCACAGGATAACCTGGCAGAGATGATCCGCCGCTTTGATGAAACg < 1:2845001/69‑1 (MQ=255) cTGGATGAATATGAATCCGATTTGTCACAGGATAACCTGGCAGAGATGATCCGCCGCTTTGATGAAACg > 1:1286606/1‑69 (MQ=255) cTGGATGAATATGAATCCGATTTGTCACAGGATAACCTGGCAGAGATGATCCGCCGCTTTGATGAAACg > 1:2828535/1‑69 (MQ=255) cTGGATGAATATGAATCCGATTTGTCACAGGATAACCTGGCAGAGATGATCCGCCGCTTTGATGAAACg > 1:2383791/1‑69 (MQ=255) cTGGATGAATATGAATCCGATTTGTCACAGGATAACCTGGCAGAGATGATCCGCCGCTTTGATGAAACg < 1:2474764/69‑1 (MQ=255) cTGGATGAATATGAATCCGATTTGTAACAGGATAACCTGGCAGAGATGATCCGCCGCTTTGATGAAACg > 1:1665108/1‑69 (MQ=255) ggATGAATATGAATCCGATTTGTCACAGGATAACCTGGCAGAGATGATCCGCCGCTTTGATGAAACgg < 1:504206/68‑1 (MQ=255) ggATGAATATGAATCCGATTTGTCACAGGATAACCTGGCAGAGATGATCCGCCGCTTTGATGAAACgg < 1:938294/68‑1 (MQ=255) ggATGAATATGAATCCGATTTGTCACAGGATAACCTGGCAGAGATGATCCGCCGCTTTGATGAAACgg < 1:2924689/68‑1 (MQ=255) ggATGAATATGAATCCGATTTGTCACAGGATAACCTGGCAGAGATGATCCGCCGCTTTGATGAAACgg < 1:1981850/68‑1 (MQ=255) atgaatCCGATTTGTCACAGGATAACCTGGCAGAGATGATCCGCCGCTTTGATGAAACGGGTCATa > 1:1346621/1‑66 (MQ=255) atgaatCCGATTTGTCACAGGATAACCTGGCAGAGATGATCCGCCGCTTTGATGAAACGGGTCATa > 1:2675941/1‑66 (MQ=255) atgaatCCGATTTGTCACAGGATAACCTGGCAGAGATGATCCGCCGCTTTGATGAAACGGGTCATa > 1:3399055/1‑66 (MQ=255) tgaatCCGATTTGTCACAGGATAACCTGGCAGAGATGATCCGCCGCttt > 1:865732/1‑49 (MQ=255) tgaatCCGATTTGTCACAGGATAACCTGGCAGAGATGATCCGCCGCTTTGATGAAACGGGTCATa > 1:3330781/1‑65 (MQ=255) tgaatCCGATTTGTCACAGGATAACCTGGCAGAGATGATCCGCCGCTTTGATGAAACGGGTCATa > 1:659180/1‑65 (MQ=255) tgaatCCGATTTGTCACAGGATAACCTGGCAGAGATGATCCGCCGCTTTGATGAAACGGGTCATa > 1:560375/1‑65 (MQ=255) tgaatCCGATTTGTCACAGGATAACCTGGCAGAGATGATCCGCCGCTTTGATGAAACGGGTCATa > 1:2300935/1‑65 (MQ=255) tgaatCCGATTTGTCACAGGATAACCTGGCAGAGATGATCCGCCGCTTTGATGAAACGGGTCATa > 1:2494922/1‑65 (MQ=255) tgaatCCGATTTGTCACAGGATAACCTGGCAGAGATGATCCGCCGCTTTGATGAAACGGGTCATa > 1:2568542/1‑65 (MQ=255) tgaatCCGATTTATCACAGGATAACCTGGCAGAGATGATCCGCCGCTTTGATGAAACGGGTCATa > 1:446214/1‑65 (MQ=255) tgaatCCGATTTATCACAGGATAACCTGGCAGAGATGATCCGCCGCTTTGATGAAACGGGTCATa > 1:2344849/1‑65 (MQ=255) tgaatCCGATTTATCACAGGATAACCTGGCAGAGATGATCCGCCGCTTTGATGAAACGGGTCATa > 1:555349/1‑65 (MQ=255) tgaatCCGATTTATCACAGGATAACCTGGCAGAGATGATCCGCCGCTTTGATGAAACGGGTCATa > 1:432413/1‑65 (MQ=255) tgaatCCGATTTATCACAGGATAACCTGGCAGAGATGATCCGCCGCTTTGATGAAACGGGTCATa > 1:1219197/1‑65 (MQ=255) tgaatCCGATTTATCACAGGATAACCTGGCAGAGATGATCCGCCGCTTTGATGAAACGGGTCATa > 1:1627505/1‑65 (MQ=255) tgaatCCGATTTATCACAGGATAACCTGGCAGAGATGATCCGCCGCTTTGATGAAACGGGTCATa > 1:640914/1‑65 (MQ=255) tgaatCCGATTTATCACAGGATAACCTGGCAGAGATGATCCGCCGCTTTGATGAAACGGGTCATa > 1:1435163/1‑65 (MQ=255) tgaatCCGATTTATCACAGGATAACCTGGCAGAGATGATCCGCCGCTTTGATGAAACGGGTCATa > 1:1236632/1‑65 (MQ=255) agaatCCGATTTATCACAGGATAACCTGGCAGAGATGATCCGCCGCTTTGATGAAACGGGTCATa > 1:1476854/2‑65 (MQ=255) tGTCACAGGATAACCTGGCAGAGATGATCCGCCGCTTTGATGAAACGGGTCATAGCCAGATCAt < 1:1144085/64‑1 (MQ=255) tGTCACAGGATAACCTGGCAGAGATGATCCGCCGCTTTGATGAAACGGGTCATAGCCAGATCAt < 1:1245614/64‑1 (MQ=255) tGTCACAGGATAACCTGGCAGAGATGATCCGCCGCTTTGATGAAACGGGTCATAGCCAGATCAt < 1:1342964/64‑1 (MQ=255) tGTCACAGGATAACCTGGCAGAGATGATCCGCCGCTTTGATGAAACGGGTCATAGCCAGATCAt < 1:658722/64‑1 (MQ=255) tGTCACAGGATAACCTGGCAGAGATGATCCGCCGCTTTGATGAAACGGGTCATAGCCAGATCAt < 1:1815657/64‑1 (MQ=255) tGTCACAGGATAACCTGGCAGAGATGATCCGCCGCTTTGATGAAACGGGTCATAGCCAGATCAt < 1:2520706/64‑1 (MQ=255) | TAGTGGGTGATGAACCGGTAGCTGTTATTTTGCCTGATGTTATTCTGGATGAATATGAATCCGATTTGTCACAGGATAACCTGGCAGAGATGATCCGCCGCTTTGATGAAACGGGTCATAGCCAGATCAT > minE/843509‑843638 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |