| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 846,580 | G→T | 100% | T340T (ACC→ACA) | cls ← | cardiolipin synthase 1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 846,580 | 0 | G | T | 87.8% | 97.5 / 7.0 | 49 | T340T (ACC→ACA) | cls | cardiolipin synthase 1 |
| Reads supporting (aligned to +/- strand): ref base G (5/1); new base T (0/43); total (5/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.15e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGA > minE/846512‑846640 | tCCACCCCGCGCTGCGCTGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTg < 1:260101/69‑1 (MQ=255)tCCACCCCGCGCTGCGCTGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTg < 1:3418119/69‑1 (MQ=255)tCCACCCCGCGCTGCGCTGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTg < 1:126143/69‑1 (MQ=255)tCCACCCCGCGCTGCGCTGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTg < 1:2156035/69‑1 (MQ=255)tCCACCCCGCGCTGCGCTGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTg < 1:1987951/69‑1 (MQ=255)tCCACCCCGCGCTGCGCTGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTg < 1:1901364/69‑1 (MQ=255)tCCACCCCGCGCTGCGCTGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTg < 1:1897524/69‑1 (MQ=255) cACCCCGCGCTGCTCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGt > 1:1337100/1‑69 (MQ=255) cACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGt > 1:2648960/1‑69 (MQ=255) cACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGt > 1:1498690/1‑69 (MQ=255) cACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGt > 1:692710/1‑69 (MQ=255) cgcgCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAATAGGGCGTTGTCATGa < 1:2286005/69‑1 (MQ=255) cgcgCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAATAGGGCGTTGTCATGa < 1:236389/69‑1 (MQ=255) cgcgCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAATAGGGCGTTGTCATGa < 1:3075552/69‑1 (MQ=255) cgcgCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAATAGGGCGTTGTCATGa < 1:1676225/69‑1 (MQ=255) cgcgCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAATAGGGCGTTGTCATGa < 1:691025/69‑1 (MQ=255) cgcgCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAATAGGGCGTTGTCATGa < 1:1564788/69‑1 (MQ=255) cgcgCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAATAGGGCGTTGTCATGa < 1:1496899/69‑1 (MQ=255) cgcgCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAATAGGGCGTTGTCATGa < 1:3328528/69‑1 (MQ=255) cgcgCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAATAGGGCGTTGTCATGa < 1:128855/69‑1 (MQ=255) cgcgCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAATAGGGCGTTGTCATGa < 1:1159157/69‑1 (MQ=255) cgcgCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAATAGGGCGTTGTCATGa < 1:396536/69‑1 (MQ=255) gcgcTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAATAGGGCGTTGTCATGa < 1:2867856/68‑1 (MQ=255) ccgccgTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAATAGGGCGTTGTCATGa < 1:56531/60‑1 (MQ=255) tcatcGCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGCAGTCaat < 1:3305413/69‑1 (MQ=255) gCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGc > 1:186642/1‑69 (MQ=255) ttGGCACAAAATAGGGCGTTGTCATGATCAAATATTCACGAGCCGAATAAGCAGCAGTCAATAATGCCt < 1:2354671/69‑1 (MQ=255) ttGGCACAAAATAGGGCGTTGTCATGATCAAATATTCACGAGCCGAATAAGCAGCAGTCAATAATGCCt < 1:533570/69‑1 (MQ=255) ttGGCACAAAATAGGGCGTTGTCATGATCAAATATTCACGAGCCGAATAAGCAGCAGTCAATAATGCCt < 1:415566/69‑1 (MQ=255) ttGGCACAAAATAGGGCGTTGTCATGATCAAATATTCACGAGCCGAATAAGCAGCAGTCAATAATGCCt < 1:1021632/69‑1 (MQ=255) ttGGCACAAAATAGGGCGTTGTCATGATCAAATATTCACGAGCCGAATAAGCAGCAGTCAATAATGCCt < 1:3403865/69‑1 (MQ=255) ttGGCACAAAATAGGGCGTTGTCATGATCAAATATTCACGAGCCGAATAAGCAGCAGTCAATAATGCCt < 1:3358256/69‑1 (MQ=255) ttGGCACAAAATAGGGCGTTGTCATGATCAAATATTCACGAGCCGAATAAGCAGCAGTCAATAATGCCt < 1:1408674/69‑1 (MQ=255) ttGGCACAAAATAGGGCGTTGTCATGATCAAATATTCACGAGCCGAATAAGCAGCAGTCAATAATGCCt < 1:1607478/69‑1 (MQ=255) ttGGCACAAAATAGGGCGTTGTCATGATCAAATATTCACGAGCCGAATAAGCAGCAGTCAATAATGCCt < 1:2941981/69‑1 (MQ=255) ttGGCACAAAATAGGGCGTTGTCATGATCAAATATTCACGAGCCGAATAAGCAGCAGTCAATAATGCCt < 1:1939790/69‑1 (MQ=255) ttGGCACAAAATAGGGCGTTGTCATGATCAAATATTCACGAGCCGAATAAGCAGCAGTCAATAATGCCt < 1:2617949/69‑1 (MQ=255) ttGGCACAAAATAGGGCGTTGTCATGATCAAATATTCACGAGCCGAATAAGCAGCAGTCAATAATGCCt < 1:2605952/69‑1 (MQ=255) ttGGCACAAAATAGGGCGTTGTCATGATCAAATATTCACGAGCCGAATAAGCAGCAGTCAATAATGCCt < 1:952231/69‑1 (MQ=255) ttGGCACAAAATAGGGCGTTGTCATGATCAAATATTCACGAGCCGAATAAGCAGCAGTCAATAATGCCt < 1:222558/69‑1 (MQ=255) ttGGCACAAAATAGGGCGTTGTCATGATCAAATATTCACGAGCCGAATAAGCAGCAGGCAATAATGCCt < 1:1009955/69‑1 (MQ=255) ttGGCACAAAATAGGGCGTTGTCATAATCAAATATTCACGAGCCGAATAAGCAGCAGTCAATAATGCCt < 1:1183313/69‑1 (MQ=255) tGGCTCAAAATTGGGCGTTGTCATGATCAAATATTCACGAGCCGAATAAGCTGCAGTCAATAATGCCt < 1:21917/68‑1 (MQ=255) tGGCACAATATAGGGCGTTGTCATGATCAAATATTCACGAGCCGAATAAGCAGCAGTCAATAATGCCt < 1:804824/68‑1 (MQ=255) tGGCACAAAATAGGGCGTTGTCATGATCAAATATTCACGAGCCGAATAAGCAGCAGTCAATAATGCCt < 1:2487152/68‑1 (MQ=255) tGGCACAAAATAGGGCGTTGTCATGATCAAATATTCACGAGCCGAATAAGCAGCAGTCAATAATGCCt < 1:560420/68‑1 (MQ=255) atAGGGCGTTGTCATGATCAAATATTCACGAGCCGAATAAGCAGCAGTCAATAATGCCT‑GTGAATgaga < 1:1644044/68‑1 (MQ=255) tAGGGCGTTGTCATGATCAAATATTCACGAGCCGAATAAGCAGCAGTCAATAATGCCTGGTGAATgaga < 1:784243/69‑1 (MQ=255) tAGGGCGTTGTCATGATCAAATATTCACGAGCCGAATAAGCAGCAGTCAATAATGCCTGGTGAATgaga < 1:953616/69‑1 (MQ=255) tAGGGCGTTGTCATGATCAAATATTCACGAGCCGAATAAGCAGCAGTCAATAATGCCTGGTGAATgaga < 1:655541/69‑1 (MQ=255) tAGGGCGTTGTCATGATCAAATATTCACGAGCCGAATAAGCAGCAGTCAATAATGCCTGGTGAATgaga < 1:3109345/69‑1 (MQ=255) tAGGGCGTTGTCATGATCAAATATTCACGAGCCGAATAAGCAGCAGTCAATAATGCCTGGTGAATgaga < 1:2995070/69‑1 (MQ=255) tAGGGCGTTGTCATGATCAAATATTCACGAGCCGAATAAGCAGCAGTCAATAATGCCTGGTGAATgaga < 1:2553362/69‑1 (MQ=255) tAGGGCGTTGTCATGATCAAATATTCACGAGCCGAATAAGCAGCAGTCAATAATGCCTGGTGAATgaga < 1:2186549/69‑1 (MQ=255) tAGGGCGTTGTCATGATCAAATATTCACGAGCCGAATAAGCAGCAGTCAATAATGCCTGGTGAATgaga < 1:2185014/69‑1 (MQ=255) aGGGCGTTGTCATGATCAAATATTCACGAGCCGAATAAGCAGCAGTCAATAATGCCTGGTGAATgaga < 1:3285443/68‑1 (MQ=255) | TCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGA > minE/846512‑846640 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |