| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 849,820 | G→A | 100% | intergenic (‑1/‑223) | yciI ← / → tonB | hypothetical protein/membrane spanning protein in TonB‑ExbB‑ExbD complex |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 849,820 | 0 | G | A | 80.8% | 41.3 / 3.8 | 31 | intergenic (‑1/‑223) | yciI/tonB | hypothetical protein/membrane spanning protein in TonB‑ExbB‑ExbD complex |
| Reads supporting (aligned to +/- strand): ref base G (4/2); new base A (0/25); total (4/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.77e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CGTTTTTCGAGGCTATCAGCTTTGTCTTGAGCGTAAATAACATACAACACGGGCACAACTCCTTGTTCGGGAAAGTTGTAAAGTACGTTATTTGAAAGGGCGAAGATCTGCAA > minE/849770‑849882 | cGTTTTTCGAGGCTATCAGCTTTGTCTTGAGCGTAAATAACATACAACACGGGCACAACTCCTTGTTc > 1:615662/1‑68 (MQ=255) tGTCTTGAGCGTAAATAACATACAACACGGGCACAACTCCTTGTTCGGGAAAGTTGTAAAGTACGTTa < 1:1309046/68‑1 (MQ=255) tAAATAACATACAACACGGGCACAACTCCTTGTTCGGGAAAGTTGTAAAGTACGTTATTTGAAAGGGCg < 1:875364/69‑1 (MQ=255) aaTAACATACAACACAGGCACAACTCCTTGTTCGTGAAAGTTGTa < 1:1918149/45‑1 (MQ=255) aaTAACATACAACACAGGCACAACTCCTTGTTCGGGAAAGTTGTa < 1:2427077/45‑1 (MQ=255) aaTAACATACAACACAGGCACAACTCCTTGTTCGGGAAAGTTGTa < 1:807962/45‑1 (MQ=255) aaTAACATACAACACAGGCACAACTCCTTGTTCGGGAAAGTTGTa < 1:487225/45‑1 (MQ=255) aaTAACATACAACACAGGCACAACTCCTTGTTCGGGAAAGTTGTa < 1:456123/45‑1 (MQ=255) aaTAACATACAACACAGGCACAACTCCTTGTTCGGGAAAGTTGTa < 1:3085071/45‑1 (MQ=255) aaTAACATACAACACAGGCACAACTCCTTGTTCGGGAAAGTTGTa < 1:3070775/45‑1 (MQ=255) aaTAACATACAACACAGGCACAACTCCTTGTTCGGGAAAGTTGTa < 1:301997/45‑1 (MQ=255) aaTAACATACAACACAGGCACAACTCCTTGTTCGGGAAAGTTGTa < 1:2965529/45‑1 (MQ=255) aaTAACATACAACACAGGCACAACTCCTTGTTCGGGAAAGTTGTa < 1:2869801/45‑1 (MQ=255) aaTAACATACAACACAGGCACAACTCCTTGTTCGGGAAAGTTGTa < 1:2868371/45‑1 (MQ=255) aaTAACATACAACACAGGCACAACTCCTTGTTCGGGAAAGTTGTa < 1:2771694/45‑1 (MQ=255) aaTAACATACAACACAGGCACAACTCCTTGTTCGGGAAAGTTGTa < 1:2403627/45‑1 (MQ=255) aaTAACATACAACACAGGCACAACTCCTTGTTCGGGAAAGTTGTa < 1:2369221/45‑1 (MQ=255) aaTAACATACAACACAGGCACAACTCCTTGTTCGGGAAAGTTGTa < 1:2290374/45‑1 (MQ=255) aaTAACATACAACACAGGCACAACTCCTTGTTCGGGAAAGTTGTa < 1:2246480/45‑1 (MQ=255) aaTAACATACAACACAGGCACAACTCCTTGTTCGGGAAAGTTGTa < 1:211867/45‑1 (MQ=255) aaTAACATACAACACAGGCACAACTCCTTGTTCGGGAAAGTTGTa < 1:2076010/45‑1 (MQ=255) aaTAACATACAACACAGGCACAACTCCTTGTTCGGGAAAGTTGTa < 1:2019925/45‑1 (MQ=255) aaTAACATACAACACAGGCACAACTCCTTGTTCGGGAAAGTTGTa < 1:1863019/45‑1 (MQ=255) aaTAACATACAACACAGGCACAACTCCTTGTTCGGGAAAGTTGTa < 1:1466385/45‑1 (MQ=255) aaTAACATACAACACAGGCACAACTCCTTGTTCGGGAAAGTTGTa < 1:1381144/45‑1 (MQ=255) aaTAACATACAACACAGGCACAACTCCTTGTTCGGGAAAGTTGTa < 1:1345620/45‑1 (MQ=255) aTAACATACAACACAGGCACAACTCCTTGTTCGGGAAAGTTGTa < 1:2216484/44‑1 (MQ=255) tAACATTCAACACAGGCACAACTCCTTGTTCGGGATAGTTGTa < 1:3241899/43‑1 (MQ=255) aaCATACAACACGGGCACAACTCCTTGTTCGGGAAAGTTGTAAAGTACGTTATTTGAAAGGGCGAAGAt > 1:1333860/1‑69 (MQ=255) caacaCGGGCACAACTCCTTGTTCGGGAAAGTTGTAAAGTACGTTATTTGAAAGGGCGAAGATCTGCaa > 1:3239806/1‑69 (MQ=255) caacaCGGGCACAACTCCTTGTTCGGGAAAGTTGTAAAGTACGTTATTTGAAAGGGCGAAGATCTGCaa > 1:776334/1‑69 (MQ=255) | CGTTTTTCGAGGCTATCAGCTTTGTCTTGAGCGTAAATAACATACAACACGGGCACAACTCCTTGTTCGGGAAAGTTGTAAAGTACGTTATTTGAAAGGGCGAAGATCTGCAA > minE/849770‑849882 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |