| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 851,033 | A→G | 26.2% | T56T (ACT→ACC) | yciA ← | predicted hydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 851,033 | 0 | A | G | 26.2% | 111.6 / 38.9 | 99 | T56T (ACT→ACC) | yciA | predicted hydrolase |
| Reads supporting (aligned to +/- strand): ref base A (27/46); new base G (23/3); total (50/49) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.50e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.81e-01 | |||||||||||
Only 100 of 102 total aligned reads displayed.
CGTGCATAGCAGCACACCACATCGCCGACCGCAACCGGCCGTAAGAAAGTCATTCCTTCAACCCGCACAGTCACTACGCGACCGTGGGCAATTTCTTTTGCCAGAATAGCGCCGCCAATATCCATTTGTGACA > minE/850965‑851097 | cGTGCATAGCAGCACACCACATCGCCGACCGCAACCGGCCGTAAGAAAGTCATTCCTTCAACCCGcaca < 1:2688002/69‑1 (MQ=255)cGTGCATAGCAGCACACCACATCGCCGACCGCAACCGGCCGTAAGAAAGTCATTCCTTCAACCCGcaca < 1:3099488/69‑1 (MQ=255)cGTGCATAGCAGCACACCACATCGCCGACCGCAACCGGCCGTAAGAAAGTCATTCCTTCAACCCGcaca < 1:1949463/69‑1 (MQ=255) tGCATAGCAGCACACCACATCGCCGACCGCAACCGGCCGTAAGAAAGTCATTCCTTCAACCCGCACAGt < 1:2727414/69‑1 (MQ=255) tGCATAGCAGCACACCACATCGCCGACCGCAACCGGCCGTAAGAAAGTCATTCCTTCAACCCGCACAGt < 1:498727/69‑1 (MQ=255) tGCATAGCAGCACACCACATCGCCGACCGCAACCGGCCGTAAGAAAGTCATTCCTTCAACCCGCACAGt < 1:144221/69‑1 (MQ=255) tGCATAGCAGCACACCACATCGCCGACCGCAACCGGCCGTAAGAAAGTCATTCCTTCAACCCGCACAGt < 1:1906074/69‑1 (MQ=255) tGCATAGCAGCACACCACATCGCCGACCGCAACCGGCCGTAAGAAAGTCATTCCTTCAACCCGCACAGt < 1:1583689/69‑1 (MQ=255) gcagcaCACCACATCGCCGACCGCAACCGGCCGTAAGAAAGTCATTCCTTCAACCCTCACAGt < 1:441426/63‑1 (MQ=255) gcagcaCACCACATCGCCGACCGCAACCGGCCGTAAGAAAGTCATTCCTTCAACCCGCACAGt < 1:3302817/63‑1 (MQ=255) gcagcaCACCACATCGCCGACCGCAACCGGCCGTAAGAAAGTCATTCCTTCAACCCGCACAGt < 1:1503848/63‑1 (MQ=255) gcagcaCACCACATCGCCGACCGCAACCGGCCGTAAGAAAGTCATTCCTTCAACCCGCACAGt < 1:556885/63‑1 (MQ=255) gcagcaCACCACATCGCCGACCGCAACCGGCCGTAAGAAAGTCATTCCTTCAACCCGCACAGt < 1:3255261/63‑1 (MQ=255) gcagcaCACCACATCGCCGACCGCAACCGGCCGTAAGAAAGTCATTCCTTCAACCCGCACAGt < 1:1526783/63‑1 (MQ=255) gcagcaCACCACATCGCCGACCGCAACCGGCCGTAAGAAAGTCATTCCTTCAACCCGCACAGt < 1:731620/63‑1 (MQ=255) gcagcaCACCACATCGCCGACCGCAACCGGCCGTAAGAAAGTCATTCCTTCAACCCGCACAGt < 1:1180266/63‑1 (MQ=255) gcagcaCACCACATCGCCGACCGCAACCGGCCGTAAGAAAGTCATTCCTTCAACCCGCACAGt < 1:2251821/63‑1 (MQ=255) gcagcaCACCACATCGCCGACCGCAACCGGCCGTAAGAAAGTCATTCCTTCAACCCGCACAGt < 1:2431586/63‑1 (MQ=255) gcagcaCACCACATCGCCGACCGCAACCGGCCGTAAGAAAGTCATTCCTTCAACCCGCACAGt < 1:837487/63‑1 (MQ=255) gcagcaCACCACATCGCCGACCGCAACCGGCCGTAAGAAAGTCATTCCTTCAACCCGCACAGTCACTa > 1:1894939/1‑68 (MQ=255) gcagcaCACCACATCGCCGACCGCAACCGGCCGTAAGAAAGTCATTCCTTCAACCCGCACAGTCACTAc > 1:337072/1‑69 (MQ=255) gcagcaCACCACATCGCCGACCGCAACCGGCCGTAAGAAAGTCATTCCTTCAACCCGCACAGTCACTAc > 1:917955/1‑69 (MQ=255) gcagcaCACCACATCGCCGACCGCAACCGGCCGTAAGAAAGTCATTCCTTCAACCCGCACAGTCACTAc > 1:1715348/1‑69 (MQ=255) gcagcaCACCACATCGCCGACCGCAACCGGCCGTAAGAAAGTCATTCCTTCAACCCGCACAGTCACTAc > 1:1906415/1‑69 (MQ=255) gcagcaCACCACATCGCCGACCGCAACCGGCCGTAAGAAAGTCATTCCTTCAACCCGCACAGTCACTAc > 1:2149590/1‑69 (MQ=255) gcagcaCACCACATCGCCGACCGCAACCGGCCGTAAGAAAGTCATTCCTTCAACCCGCACAGTCACTAc > 1:3012439/1‑69 (MQ=255) gcagcaCACCACATCGCCGACCGCAACCGGCCGTAAGAAAGTCATTCCTTCAACCCGCACAGTCACTAc > 1:2965182/1‑69 (MQ=255) gcagcaCACCACATCGCCGACCGCAACCGGCCGTAAGAAAGTCATTCCTTCAACCCGCACAGTCACTAc > 1:2917679/1‑69 (MQ=255) gcagcaCACCACATCGCCGACCGCAACCGGCCGTAAGAAAGTCATTCCTTCAACCCGCACAGTCACTAc > 1:373536/1‑69 (MQ=255) gcagcaCACCACATCGCCGACCGCAACCGGCCGTAAGAAAGTCATTCCTTCAACCCGCACAGTCACTAc > 1:1158978/1‑69 (MQ=255) gcagcaCACCACATCGCCGACCGCAACCGGCCGTAAGAAAGTCATTCCTTCAACCCGCACAGTCACTAc > 1:1330396/1‑69 (MQ=255) gcagcaCACCACATCGCCGACCGCAACCGGCCGTAAGAAAGTCATTCCTTCAACCCGCACAGTCACTAc > 1:1211419/1‑69 (MQ=255) gcaacaCACCACATCGCCGACCGCAACCGGCCGTAAGAAAGTCATTCCTTCAACCCGCACAGTCACTAc > 1:3277004/1‑69 (MQ=255) agcaCACCACATCGCCGACCGCAACCGGCCGTAAGAAAGTCATTCCTTCAACCCGCACAGTCACTAcgc > 1:2731749/1‑69 (MQ=255) agcaCACCACATCGCCGACCGCAACCGGCCGTAAGAAAGTCATTCCTTCAACCCGCACAGTCACTAcgc > 1:1151886/1‑69 (MQ=255) cacaTCGCCGACCGCAACCGGCCGTAAGAAAGTCATTCCTTCAACCCGCAGAGTCAc < 1:442485/57‑1 (MQ=255) cacaTCGCCGACCGCAACCGGCCGTAAGAAAGTCATTCCTTCAACCCGCACGGTCACTACACGAc < 1:1120890/65‑1 (MQ=255) cacaTCGCCGACCGCAACCGGCCGTAAGAAAGTCATTCCTTCAACCCGCACAGTCAc < 1:2693020/57‑1 (MQ=255) cacaTCGCCGACCGCAACCGGCCGTAAGAAAGTCATTCCTTCAACCCGCACAGTCAc < 1:2476086/57‑1 (MQ=255) cacaTCGCCGACCGCAACCGGCCGTAAGAAAGTCATTCCTTCAACCCGCACAGTCAc < 1:1200088/57‑1 (MQ=255) cacaTCGCCGACCGCAACCGGCCGTAAGAAAGTCATTCCTTCAACCCGCACAGTCAc < 1:3053991/57‑1 (MQ=255) cacaTCGCCGACCGCAACCGGCCGTAAGAAAGTCATTCCTTCAACCCGCACAGTCAc < 1:3082247/57‑1 (MQ=255) cacaTCGCCGACCGCAACCGGCCGTAAGAAAGTCATTCCTTCAACCCGCACAGTCAc < 1:2082228/57‑1 (MQ=255) cacaTCGCCGACCGCAACCGGCCGTAAGAAAGTCATTCCTTCAACCCGCACAGTCAc < 1:2048645/57‑1 (MQ=255) cacaTCGCCGACCGCAACCGGCCGTAAGAAAGTCATTCCTTCAACCCGCACAGTCAc < 1:181996/57‑1 (MQ=255) cacaTCGCCGACCGCAACCGGCCGTAAGAAAGTCATTCCTTCAACCCGCACAGTCAc < 1:3160168/57‑1 (MQ=255) cacaTCGCCGACCGCAACCGGCCGTAAGAAAGTCATTCCTTCAACCCGCACAGTCAc < 1:191539/57‑1 (MQ=255) cacaTCGCCGACCGCAACCGGCCGTAAGAAAGTCATTCCTTCAACCCGCACAGTCAc < 1:623825/57‑1 (MQ=255) cacaTCGCCGACCGCAACCGGCCGTAAGAAAGTCATTCCTTCAACCCGCACAGTCAc < 1:188471/57‑1 (MQ=255) cacaTCGCCGACCGCAACCGGCCGTAAGAAAGTCATTCCTTCAACCCGCACAGTCAc < 1:1428199/57‑1 (MQ=255) acaTCGCCGACCGCAACCGGCCGTAAGAAAGTCATTCCTTCAACCCGCACGGTCACTACACGAc < 1:1493995/64‑1 (MQ=255) acaTCGCCGACCGCAACCGGCCGTAAGAAAGTCATTCCTTCAACCCGCACGGTCACTACACGAc < 1:2471109/64‑1 (MQ=255) aTCGCCGACCGCAACCGGCCGTAAGAAAGTCATTCCTTCAACCCGCACAGt < 1:2787092/51‑1 (MQ=255) tCGCCGACCGCAACCGGCCGTAAGAAAGTCATTCCTTCAACCCGCACAGt < 1:652975/50‑1 (MQ=255) cGACCGCAACCGGCCGTAAGAAAGTCATTCCTTCAACCCGCACAGTCAc < 1:116752/49‑1 (MQ=255) ctgCCGTAAGAAAGTCATTCCTTCAACCCGCACAGTCACTACGCGACCGTGGGCAATTTCTTTTGCCAg < 1:3422524/67‑1 (MQ=255) cGGCCGTAAGAAAGTCATTCCTTCAACCCGCACAGTCACTACGCGACCGTGGGCAATTTCTTTTGCCAg < 1:1417919/69‑1 (MQ=255) cGGCCGTAAGAAAGTCATTCCTTCAACCCGCACAGTCACTACGCGACCGTGGGCAATTTCTTTTGCCAg < 1:3100567/69‑1 (MQ=255) cGGCCGTAAGAAAGTCATTCCTTCAACCCCCACAGTCACTACGCGACCGTGGGCAATTTCTTTTGCCAg < 1:1065536/69‑1 (MQ=255) cGGCCGTAAGAAAGTCATTCCTACAACCCGCACAGTCACTACGCGACCGTGGGCAATTTCTTTTGCCAg < 1:1058667/69‑1 (MQ=255) cGGCCGTAAGAAAGTCATTCCATCAACCCGCACAGTCACTACGCGACCGTGGGCAATTTCTTTTGCCAg < 1:2242693/69‑1 (MQ=255) ggCCGTAAGAAAGTCATTCCTTCAACCCGCACAGTCACTACGCGACCGTGGGCAATTTCTTTTGCCAg > 1:1779479/1‑68 (MQ=255) ggCCGTAAGAAAGTCATTCCTTCAACCCGCACAGTCACTACGCGACCGTGGGCAATTTCTTTTGCCAg > 1:1065227/1‑68 (MQ=255) ccGTAAGAAAGTCATTCCTTCAACCCGCACAGTCACTACGCGACCGTGGGCAATTTCTTTTGCCAGAAt < 1:196863/69‑1 (MQ=255) tCATTCCTTCAACCCGCACAGTCACTACGCGACCGTGGGCAATTTCTTTTGCCAGAATAGCGCCGCCaa > 1:2589372/1‑69 (MQ=255) tCATTCCTTCAACCCGCACAGTCACTACGCGACCGTGGGCAATTTCTTTTGCCAGAATAGCGCCGCCaa > 1:2163290/1‑69 (MQ=255) cATTCCTTCAACCCGCACAGTCACTACGCGACCGTGGGCAATTTCTTTTGCCAGAATAGCGCCGCCAat > 1:787755/1‑69 (MQ=255) cATTCCTTCAACCCGCACAGTCACTACGCGACCGTGGGCAATTTCTTTTGCCAGAATAGCGCCGCCAat > 1:2445577/1‑69 (MQ=255) cATTCCTTCAACCCGCACAGTCACTACGCGACCGTGGGCAATTTCTTTTGCCAGAATAGCGCCGCCAat > 1:415248/1‑69 (MQ=255) cATTCCTTCAACCCGCACAGTCACTACGCGACCGTGGGCAATTTCTTTTGCCAGAATAGCGCCGCCAat > 1:3182290/1‑69 (MQ=255) cATTCCTTCAACCCGCACAGTCACTACGCGACCGTGGGCAATTTCTTTTGCCAGAATAGCGCCGCCAat > 1:2019077/1‑69 (MQ=255) tCCTTCAACCCGCACGGTCACTACACGACCATgggg > 1:721540/1‑35 (MQ=25) tCCTTCAACCCGCACGGTCACTACACGACCATGGGc > 1:553606/1‑36 (MQ=37) tCCTTCAACCCGCACGGTCACTACACGACCATGGGc > 1:1633796/1‑36 (MQ=37) tCCTTCAACCCGCACGGTCACTACACGACCATGGGc > 1:1605331/1‑36 (MQ=37) tCCTTCAACCCGCACGGTCACTACACGACCATGGGc > 1:1535841/1‑36 (MQ=37) tCCTTCAACCCGCACGGTCACTACACGACCATGGGc > 1:1507194/1‑36 (MQ=37) tCCTTCAACCCGCACGGTCACTACACGACCATGGGc > 1:3269759/1‑36 (MQ=37) tCCTTCAACCCGCACGGTCACTACACGACCATGGGc > 1:1434385/1‑36 (MQ=37) tCCTTCAACCCGCACGGTCACTACACGACCATGGGc > 1:136036/1‑36 (MQ=37) tCCTTCAACCCGCACGGTCACTACACGACCATGGGc > 1:1266828/1‑36 (MQ=37) tCCTTCAACCCGCACGGTCACTACACGACCATGGGc > 1:1249013/1‑36 (MQ=37) tCCTTCAACCCGCACGGTCACTACACGACCATGGGc > 1:778052/1‑36 (MQ=37) tCCTTCAACCCGCACGGTCACTACACGACCATGGGc > 1:1008285/1‑36 (MQ=37) tCCTTCAACCCGCACGGTCACTACACGACCATGGGc > 1:1793633/1‑36 (MQ=37) tCCTTCAACCCGCACGGTCACTACACGACCATGGGc > 1:1904338/1‑36 (MQ=37) tCCTTCAACCCGCACGGTCACTACACGACCATGGGc > 1:1976684/1‑36 (MQ=37) tCCTTCAACCCGCACGGTCACTACACGACCATGGGc > 1:3016844/1‑36 (MQ=37) tCCTTCAACCCGCACGGTCACTACACGACCATGGGc > 1:3006899/1‑36 (MQ=37) tCCTTCAACCCGCACGGTCACTACACGACCATGGGc > 1:3000926/1‑36 (MQ=37) tCCTTCAACCCGCACGGTCACTACACGACCATGGGc > 1:2379315/1‑36 (MQ=37) tCCTTCAACCCGCACGGTCACTACACGACCATGGGc > 1:2922481/1‑36 (MQ=37) tCCTTCAACCCGCACGGTCACTACACGACCATGGGc > 1:247747/1‑36 (MQ=37) tCCTTCAACCCGCACGGTCACTACACGACCATGGGc > 1:253272/1‑36 (MQ=37) ccTTCAACCCGCACAGTCACTACGCGACCGTGGGCAATTTCTTTTGCCAGAATAGCGCCGCCAATATcc > 1:1897177/1‑69 (MQ=255) ccTTCAACCCGCACAGTCACTACGCGACCGTGGGCAATTTCTTTTGCCAGAATAGCGCCGCCAATATcc > 1:2849274/1‑69 (MQ=255) gCACAGTCACTACGCGACCGTGGGCAATTTCTTTTGCCAGAATAGCGCCGCCAATATCCATTTGTGACa < 1:30582/69‑1 (MQ=255) gCACAGTCACTACGCGACCGTGGGCAATTTCTTTTGCCAGAATAGCGCCGCCAATATCCATTTGTGACa < 1:1490792/69‑1 (MQ=255) gCACAGTCACTACGCGACCGTGGGCAATTTCTTTTGCCAGAATAGCGCCGCCAATATCCATTTGTGACa < 1:218347/69‑1 (MQ=255) gCACAGTCACAACGCGACCGTGGGCAATTTCTTTTGCCAGAATAGCGCCGCCAATATCCATTTGTGACa < 1:2878724/69‑1 (MQ=255) | CGTGCATAGCAGCACACCACATCGCCGACCGCAACCGGCCGTAAGAAAGTCATTCCTTCAACCCGCACAGTCACTACGCGACCGTGGGCAATTTCTTTTGCCAGAATAGCGCCGCCAATATCCATTTGTGACA > minE/850965‑851097 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |