| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 851,042 | G→A | 50.0% | R53R (CGC→CGT) | yciA ← | predicted hydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 851,042 | 0 | G | A | 50.0% | ‑4.2 / 45.2 | 52 | R53R (CGC→CGT) | yciA | predicted hydrolase |
| Reads supporting (aligned to +/- strand): ref base G (14/12); new base A (23/3); total (37/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.28e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01 | |||||||||||
AGCACACCACATCGCCGACCGCAACCGGCCGTAAGAAAGTCATTCCTTCAACCCGCACAGTCACTACGCGACCGTGGGCAATTTCTTTTGCCAGAATAGCGCCGCCAATATCCATTTGTGACATTAACCA > minE/850975‑851104 | agcaCACCACATCGCCGACCGCAACCGGCCGTAAGAAAGTCATTCCTTCAACCCGCACAGTCACTAcgc > 1:1151886/1‑69 (MQ=255)agcaCACCACATCGCCGACCGCAACCGGCCGTAAGAAAGTCATTCCTTCAACCCGCACAGTCACTAcgc > 1:2731749/1‑69 (MQ=255) cacaTCGCCGACCGCAACCGGCCGTAAGAAAGTCATTCCTTCAACCCGCACGGTCACTACACGAc < 1:1120890/65‑1 (MQ=255) acaTCGCCGACCGCAACCGGCCGTAAGAAAGTCATTCCTTCAACCCGCACGGTCACTACACGAc < 1:1493995/64‑1 (MQ=255) acaTCGCCGACCGCAACCGGCCGTAAGAAAGTCATTCCTTCAACCCGCACGGTCACTACACGAc < 1:2471109/64‑1 (MQ=255) ctgCCGTAAGAAAGTCATTCCTTCAACCCGCACAGTCACTACGCGACCGTGGGCAATTTCTTTTGCCAg < 1:3422524/67‑1 (MQ=255) cGGCCGTAAGAAAGTCATTCCTTCAACCCGCACAGTCACTACGCGACCGTGGGCAATTTCTTTTGCCAg < 1:3100567/69‑1 (MQ=255) cGGCCGTAAGAAAGTCATTCCTTCAACCCGCACAGTCACTACGCGACCGTGGGCAATTTCTTTTGCCAg < 1:1417919/69‑1 (MQ=255) cGGCCGTAAGAAAGTCATTCCTTCAACCCCCACAGTCACTACGCGACCGTGGGCAATTTCTTTTGCCAg < 1:1065536/69‑1 (MQ=255) cGGCCGTAAGAAAGTCATTCCTACAACCCGCACAGTCACTACGCGACCGTGGGCAATTTCTTTTGCCAg < 1:1058667/69‑1 (MQ=255) cGGCCGTAAGAAAGTCATTCCATCAACCCGCACAGTCACTACGCGACCGTGGGCAATTTCTTTTGCCAg < 1:2242693/69‑1 (MQ=255) ggCCGTAAGAAAGTCATTCCTTCAACCCGCACAGTCACTACGCGACCGTGGGCAATTTCTTTTGCCAg > 1:1065227/1‑68 (MQ=255) ggCCGTAAGAAAGTCATTCCTTCAACCCGCACAGTCACTACGCGACCGTGGGCAATTTCTTTTGCCAg > 1:1779479/1‑68 (MQ=255) ccGTAAGAAAGTCATTCCTTCAACCCGCACAGTCACTACGCGACCGTGGGCAATTTCTTTTGCCAGAAt < 1:196863/69‑1 (MQ=255) tCATTCCTTCAACCCGCACAGTCACTACGCGACCGTGGGCAATTTCTTTTGCCAGAATAGCGCCGCCaa > 1:2589372/1‑69 (MQ=255) tCATTCCTTCAACCCGCACAGTCACTACGCGACCGTGGGCAATTTCTTTTGCCAGAATAGCGCCGCCaa > 1:2163290/1‑69 (MQ=255) cATTCCTTCAACCCGCACAGTCACTACGCGACCGTGGGCAATTTCTTTTGCCAGAATAGCGCCGCCAat > 1:3182290/1‑69 (MQ=255) cATTCCTTCAACCCGCACAGTCACTACGCGACCGTGGGCAATTTCTTTTGCCAGAATAGCGCCGCCAat > 1:415248/1‑69 (MQ=255) cATTCCTTCAACCCGCACAGTCACTACGCGACCGTGGGCAATTTCTTTTGCCAGAATAGCGCCGCCAat > 1:787755/1‑69 (MQ=255) cATTCCTTCAACCCGCACAGTCACTACGCGACCGTGGGCAATTTCTTTTGCCAGAATAGCGCCGCCAat > 1:2445577/1‑69 (MQ=255) cATTCCTTCAACCCGCACAGTCACTACGCGACCGTGGGCAATTTCTTTTGCCAGAATAGCGCCGCCAat > 1:2019077/1‑69 (MQ=255) tCCTTCAACCCGCACGGTCACTACACGACCATgggg > 1:721540/1‑35 (MQ=25) tCCTTCAACCCGCACGGTCACTACACGACCATGGGc > 1:1904338/1‑36 (MQ=37) tCCTTCAACCCGCACGGTCACTACACGACCATGGGc > 1:1008285/1‑36 (MQ=37) tCCTTCAACCCGCACGGTCACTACACGACCATGGGc > 1:778052/1‑36 (MQ=37) tCCTTCAACCCGCACGGTCACTACACGACCATGGGc > 1:553606/1‑36 (MQ=37) tCCTTCAACCCGCACGGTCACTACACGACCATGGGc > 1:1249013/1‑36 (MQ=37) tCCTTCAACCCGCACGGTCACTACACGACCATGGGc > 1:1266828/1‑36 (MQ=37) tCCTTCAACCCGCACGGTCACTACACGACCATGGGc > 1:3269759/1‑36 (MQ=37) tCCTTCAACCCGCACGGTCACTACACGACCATGGGc > 1:136036/1‑36 (MQ=37) tCCTTCAACCCGCACGGTCACTACACGACCATGGGc > 1:3016844/1‑36 (MQ=37) tCCTTCAACCCGCACGGTCACTACACGACCATGGGc > 1:3006899/1‑36 (MQ=37) tCCTTCAACCCGCACGGTCACTACACGACCATGGGc > 1:3000926/1‑36 (MQ=37) tCCTTCAACCCGCACGGTCACTACACGACCATGGGc > 1:2922481/1‑36 (MQ=37) tCCTTCAACCCGCACGGTCACTACACGACCATGGGc > 1:1434385/1‑36 (MQ=37) tCCTTCAACCCGCACGGTCACTACACGACCATGGGc > 1:247747/1‑36 (MQ=37) tCCTTCAACCCGCACGGTCACTACACGACCATGGGc > 1:1793633/1‑36 (MQ=37) tCCTTCAACCCGCACGGTCACTACACGACCATGGGc > 1:1976684/1‑36 (MQ=37) tCCTTCAACCCGCACGGTCACTACACGACCATGGGc > 1:1507194/1‑36 (MQ=37) tCCTTCAACCCGCACGGTCACTACACGACCATGGGc > 1:1633796/1‑36 (MQ=37) tCCTTCAACCCGCACGGTCACTACACGACCATGGGc > 1:2379315/1‑36 (MQ=37) tCCTTCAACCCGCACGGTCACTACACGACCATGGGc > 1:1605331/1‑36 (MQ=37) tCCTTCAACCCGCACGGTCACTACACGACCATGGGc > 1:253272/1‑36 (MQ=37) tCCTTCAACCCGCACGGTCACTACACGACCATGGGc > 1:1535841/1‑36 (MQ=37) ccTTCAACCCGCACAGTCACTACGCGACCGTGGGCAATTTCTTTTGCCAGAATAGCGCCGCCAATATcc > 1:1897177/1‑69 (MQ=255) ccTTCAACCCGCACAGTCACTACGCGACCGTGGGCAATTTCTTTTGCCAGAATAGCGCCGCCAATATcc > 1:2849274/1‑69 (MQ=255) gCACAGTCACTACGCGACCGTGGGCAATTTCTTTTGCCAGAATAGCGCCGCCAATATCCATTTGTGACa < 1:30582/69‑1 (MQ=255) gCACAGTCACTACGCGACCGTGGGCAATTTCTTTTGCCAGAATAGCGCCGCCAATATCCATTTGTGACa < 1:218347/69‑1 (MQ=255) gCACAGTCACTACGCGACCGTGGGCAATTTCTTTTGCCAGAATAGCGCCGCCAATATCCATTTGTGACa < 1:234321/69‑1 (MQ=255) gCACAGTCACTACGCGACCGTGGGCAATTTCTTTTGCCAGAATAGCGCCGCCAATATCCATTTGTGACa < 1:1490792/69‑1 (MQ=255) gCACAGTCACAACGCGACCGTGGGCAATTTCTTTTGCCAGAATAGCGCCGCCAATATCCATTTGTGACa < 1:2878724/69‑1 (MQ=255) cACTACGCGACCGTGGGCAATTTCTTTTGCCAGAATAGCGCCGCCAATATCCATTTGTGACATTaacca > 1:1423009/1‑69 (MQ=255) cACTACGCGACCGTGGGCAATTTCTTTTGCCAGAATAGCGCCGCCAATATCCATTTGTGACATTaacca > 1:2427618/1‑69 (MQ=255) cACTACGCGACCGTGGGCAATTTCTTTTGCCAGAATAGCGCCGCCAATATCCATTTGTGACATTaacca > 1:1492905/1‑69 (MQ=255) gcgACCGTGGGCAATTTCTTTTGCCAGAATAGCGCCGCCAATATCCATTTGTGACa < 1:3037269/56‑1 (MQ=255) | AGCACACCACATCGCCGACCGCAACCGGCCGTAAGAAAGTCATTCCTTCAACCCGCACAGTCACTACGCGACCGTGGGCAATTTCTTTTGCCAGAATAGCGCCGCCAATATCCATTTGTGACATTAACCA > minE/850975‑851104 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |