| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 852,069 | A→T | 43.8% | T183T (ACT→ACA) | yciC ← | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 852,069 | 0 | A | T | 43.8% | 18.4 / 67.2 | 64 | T183T (ACT→ACA) | yciC | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base A (14/22); new base T (16/12); total (30/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.07e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01 | |||||||||||
AGCAACAGTGTTTTTGCCAGCAACCAGCTCAGTACTGCGGGTGCCACCAGACGCATATTCGCCCAAGTCAGCCGCATACTGCTACGCATCGAGGCAAAAACGCCCATTTTGTCCTGTACCAACATCACC > minE/852004‑852132 | agcaACAGTGTTTTTGCCAGCAACCAGCTCAGTACTGCGGGTGCCACCAGACGCATATTCGCCCAAGTc < 1:4166/69‑1 (MQ=255)agcaACAGTCTTTTTGCCAGCAACCAGCTCAGTACTGCGGGTGCCACCAGACGCATATTCGCCCAAGTc < 1:2104421/69‑1 (MQ=255) gtTTTTGCCAGCAACCAGCTCTGTACTGCGGGTGCCACCAGACGCATATTCGCCCATGTCAGCCGCATa > 1:3292728/1‑69 (MQ=255) gtTTTTGCCAGCAACCAGCTCAGTACTGCGGGTGCCACCAGACGCATATTCGCCCATGTCAGCCGCATa > 1:2932855/1‑69 (MQ=255) gtTTTTGCCAGCAACCAGCTCAGTACTGCGGGTGCCACCAGACGCATATTCGCCCATGTCAGCCGCATa > 1:2405930/1‑69 (MQ=255) gtTTTTGCCAGCAACCAGCTCAGTACTGCGGGTGCCACCAGACGCATATTCGCCCATGTCAGCCGCATa > 1:2227986/1‑69 (MQ=255) gtTTTTGCCAGCAACCAGCTCAGTACTGCGGGTGCCACCAGACGCATATTCGCCCATGTCAGCCGCATa > 1:2429076/1‑69 (MQ=255) gtTTTTGCCAGCAACCAGCTCAGTACTGCGGGTGCCACCAGACGCATATTCGCCCATGTCAGCCGCATa > 1:1013048/1‑69 (MQ=255) gtTTTTGCCAGCAACCAGCTCAGTACTGCGGGTGCCACCAGACGCATATTCGCCCATGTCAGCCGCATa > 1:2985050/1‑69 (MQ=255) gtTTTTGCCAGCAACCAGCTCAGTACTGCGGGTGCCACCAGACGCATATTCGCCCATGTCAGCCGCATa > 1:1716052/1‑69 (MQ=255) gtTTTTGCCAGCAACCAGCTCAGTACTGCGGGTGCCACCAGACGCATATTCGCCCATGTCAGCCGCATa > 1:1706863/1‑69 (MQ=255) gtTTTTGCCAGCAACCAGCTCAGTACTGCGGGTGCCACCAGACGCATATTCGCCCATGTCAGCCGCATa > 1:447148/1‑69 (MQ=255) gtTTTTGCCAGCAACCAGCTCAGTACTGCGGGTGCCACCAGACGCATATTCGCCCATGTCAGCCGCATa > 1:1497766/1‑69 (MQ=255) gtTTTTGCCAGCAACCAGCTCAGTACTGCGGGTGCCACCAGACGCATATTCGCCCATGTCAGCCGCATa > 1:669684/1‑69 (MQ=255) gtTTTTGCCAGCAACCAGCTCAGTACTGCGGGTGCCACCAGACGCATATTCGCCCATGTCAGCCGCATa > 1:2894743/1‑69 (MQ=255) gtTTTTGCCAGCAACCAGCTCAGTACTGCGGGTGCCACCAGACGCATATTCGCCCATGTCAGCCGCATa > 1:748712/1‑69 (MQ=255) gtTTTTGCCAGCAACCAGCTCAGTACTGCGGGTGCCACCAGACGCATATTCGCCCATGTCAGCCGCATa > 1:79458/1‑69 (MQ=255) gtTTTTGCCAGCAACCAGCTCAGTACTGCGGGTGCCACCAGACGCATATTCGCCCATGTCAGCCGCATa > 1:1107003/1‑69 (MQ=255) aCCAGCTCAGTACTGCGGGTGCCACCAGACGCATATTCGCCCAAGTc < 1:490880/47‑1 (MQ=255) cAGCTCAGTACTGCGGGTGCCACCAGACGCTTATTCGCCCATGTCAGCCGCATACTGCTACGCATCGAg < 1:2944542/69‑1 (MQ=255) cAGCTCAGTACTGCGGGTGCCACCAGACGCATATTCGCCCATGTCAGCCGCATACTGCTACGCATCGAg < 1:2943326/69‑1 (MQ=255) cAGCTCAGTACTGCGGGTGCCACCAGACGCATATTCGCCCATGTCAGCCGCATACTGCTACGCATCGAg < 1:266912/69‑1 (MQ=255) cAGCTCAGTACTGCGGGTGCCACCAGACGCATATTCGCCCATGTCAGCCGCATACTGCTACGCATCGAg < 1:3158779/69‑1 (MQ=255) cAGCTCAGTACTGCGGGTGCCACCAGACGCATATTCGCCCATGTCAGCCGCATACTGCTACGCATCGAg < 1:3293038/69‑1 (MQ=255) cAGCTCAGTACTGCGGGTGCCACCAGACGCATATTCGCCCATGTCAGCCGCATACTGCTACGCATCGAg < 1:2338346/69‑1 (MQ=255) cAGCTCAGTACTGCGGGTGCCACCAGACGCATATTCGCCCATGTCAGCCGCATACTGCTACGCATCGAg < 1:2334521/69‑1 (MQ=255) cAGCTCAGTACTGCGGGTGCCACCAGACGCATATTCGCCCATGTCAGCCGCATACTGCTACGCATCGAg < 1:2143226/69‑1 (MQ=255) cAGCTCAGTACTGCGGGTGCCACCAGACGCATATTCGCCCATGTCAGCCGCATACTGCTACGCATCGAg < 1:3353718/69‑1 (MQ=255) cAGCTCAGTACTGCGGGTGCCACCAGACGCATATTCGCCCATGTCAGCCGCATACTGCTACGCATCGAg < 1:3432548/69‑1 (MQ=255) cAGCTCAGTACTGCGGGTGCCACCAGACGCATATTCGCCCATGTCAGCCGCATACTGCTACGCATCGAg < 1:178718/69‑1 (MQ=255) cAGCTCAGTACTGCGGGTGCCACCAGACGCATATTCGCCCATGTCAGCCGCATACTGCTACGCATCGAg < 1:689122/69‑1 (MQ=255) cTGCGGGTGCCACCAGACGCATATTCGCCCAAGTCAGCCGCATACTGCTACGCATCGAGGCAAAAACg > 1:3181978/1‑68 (MQ=255) cTGCGGGTGCCACCAGACGCATATTCGCCCAAGTCAGCCGCATACTGCTACGCATCGAGGCAAAAACg > 1:2425057/1‑68 (MQ=255) cTGCGGGTGCCACCAGACGCATATTCGCCCAAGTCAGCCGCATACTGCTACGCATCGAGGCAAAAACg > 1:2581054/1‑68 (MQ=255) ggTGCCACCAGACGCATATTCGCCCAAGTCAGCCGCATACTGCTACGCATCGAGGCAAAAACGCCCa > 1:3146319/1‑67 (MQ=255) ggTGCCACCAGACGCATATTCGCCCAAGTCAGCCGCATACTGCTACGCATCGAGGCAAAAACGCCCAtt > 1:838443/1‑69 (MQ=255) ggTGCCACCAGACGCATATTCGCCCAAGTCAGCCGCATACTGCTACGCATCGAGGCAAAAACGCCCAtt > 1:1092890/1‑69 (MQ=255) ggTGCCACCAGACGCATATTCGCCCAAGTCAGCCGCATACTGCTACGCATCGAGGCAAAAACGCCCAtt > 1:894237/1‑69 (MQ=255) ggTGCCACCAGACGCATATTCGCCCAAGTCAGCCGCATACTGCTACGCATCGAGGCAAAAACGCCCAt > 1:2595737/1‑68 (MQ=255) ggTGCCACCAGACGCATATTCGCCCAAGTCAGCCGCATACTGCTACGCATCGAGGCAAAAACGCCCAt > 1:2590017/1‑68 (MQ=255) ggTGCCACCAGACGCATATTCGCCCAAGTCAGCCGCATACTGCTACGCATCGAGGCAAAAACGCCAAtt > 1:883081/1‑69 (MQ=255) ggTCCCACCAGACGCATATTCGCCCAAGTCAGCCGCATACTGCTACGCATCGAGGCAAAAACGCCCAtt > 1:3265526/1‑69 (MQ=255) cGCATATTCGCCCAAGTCAGCCGCATACTGCTACGCATCGAGGCAAAAACTCCCATTTTGTCCTGTAcc < 1:457813/69‑1 (MQ=255) cGCATATTCGCCCAAGTCAGCCGCATACTGCTACGCATCGAGGCAAAAACGCCCATTTTGTCCTGTAcc < 1:1112714/69‑1 (MQ=255) cGCATATTCGCCCAAGTCAGCCGCATACTGCTACGCATCGAGGCAAAAACGCCCATTTTGTCCTGTAcc < 1:834592/69‑1 (MQ=255) cGCATATTCGCCCAAGTCAGCCGCATACTGCTACGCATCGAGGCAAAAACGCCCATTTTGTCCTGTAcc < 1:1998/69‑1 (MQ=255) cGCATATTCGCCCAAGTCAGCCGCATACTGCTACGCATCGAGGCAAAAACGCCCATTTTGTCCTGTAcc < 1:1641548/69‑1 (MQ=255) ttCGCCCAAGTCGGCCGCATACTGCTACGCATCGAGGCAAAAACGCCCATTTTGTCCTg < 1:2417502/59‑1 (MQ=255) ttCGCCCAAGTCAGCCGCATACTGCTACGCATCGAGGCAAAAACGCCCATTTTGTCCTg < 1:1019897/59‑1 (MQ=255) ttCGCCCAAGTCAGCCGCATACTGCTACGCATCGAGGCAAAAACGCCCATTTTGTCCTg < 1:2949904/59‑1 (MQ=255) ttCGCCCAAGTCAGCCGCATACTGCTACGCATCGAGGCAAAAACGCCCATTTTGTCCTg < 1:77130/59‑1 (MQ=255) ttCGCCCAAGTCAGCCGCATACTGCTACGCATCGAGGCAAAAACGCCCATTTTGTCCTg < 1:1407184/59‑1 (MQ=255) ttCGCCCAAGTCAGCCGCATACTGCTACGCATCGAGGCAAAAACGCCCATTTTGTCCTg < 1:1413698/59‑1 (MQ=255) ttCGCCCAAGTCAGCCGCATACTGCTACGCATCGAGGCAAAAACGCCCATTTTGTCCTg < 1:163169/59‑1 (MQ=255) ttCGCCCAAGTCAGCCGCATACTGCTACGCATCGAGGCAAAAACGCCCATTTTGTCCTg < 1:2122409/59‑1 (MQ=255) ttCGCCCAAGTCAGCCGCATACTGCTACGCATCGAGGCAAAAACGCCCATTTTGTCCTg < 1:2363206/59‑1 (MQ=255) ttCGCCCAAGTCAGCCGCATACTGCTACGCATCGAGGCAAAAACGCCCATTTTGTCCTg < 1:2442998/59‑1 (MQ=255) ttCGCCCAAGTCAGCCGCATACTGCTACGCATCGAGGCAAAAACGCCCATTTTGTCCTg < 1:2975711/59‑1 (MQ=255) ttCGCCCAAGTCAGCCGCATACTGCTACGCATCGAGGCAAAAACGCCCATTTTGTCCTGTACCAACAt > 1:3163621/1‑68 (MQ=255) ttCGCCCAAGTCAGCCGCATACTGCTACGCATCGAGGCAAAAACGCCCATTTTGTCCTGTACCAACATc > 1:2123969/1‑69 (MQ=255) ttCGCCCAAGTCAGCCGCATACTGCTACGCATCGAGGCAAAAACGCCCATTTTGTCCTGTACCAACATc > 1:1385614/1‑69 (MQ=255) cccAAGTCAGCCGCATACTGCTACGCATCGAGGCAAAAACGCCCATTTTGTCCTGTACCAACATCAcc < 1:1604994/68‑1 (MQ=255) cccAAGTCAGCCGCATACTGCTACGCATCGAGGCAAAAACGCCCATTTTGTCCTGTACCAACATCAcc < 1:1418488/68‑1 (MQ=255) cccAAGTCAGCCGCATACTGCTACGCATCGAGGCAAAAACGCCCATTTTGTCCTGTACCAACATCAcc < 1:2699143/68‑1 (MQ=255) | AGCAACAGTGTTTTTGCCAGCAACCAGCTCAGTACTGCGGGTGCCACCAGACGCATATTCGCCCAAGTCAGCCGCATACTGCTACGCATCGAGGCAAAAACGCCCATTTTGTCCTGTACCAACATCACC > minE/852004‑852132 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |