| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 853,846 | G→A | 29.2% | Y111Y (TAC→TAT) | yciE ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 853,846 | 0 | G | A | 29.2% | 57.5 / 23.2 | 58 | Y111Y (TAC→TAT) | yciE | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (11/30); new base A (10/7); total (21/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.47e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01 | |||||||||||
ATCGCTTCGATGGTTGGAATTGAAGCTGTATCACCGGCATTTTTTGCTGCTGCTAATAGTGAGGTGTAACAGGCGATTTCAAATTGCTCGAAGACATATCCGCTAATAGAGCCTTTGACTATTTCATCAG > minE/853781‑853910 | aTCGCTTCGATGGTTGGAATTGAAGCTGTATCACCGGCATTTTTTGCTGCTGCTAATAGTGAGGTGTa < 1:1672889/68‑1 (MQ=255) gAAGCTGTATCACCGGCATTTTTTGCTGCTGCTAAGAGTGAGGTATAACAGGCGATTTCAAATTGCTCg < 1:2900052/69‑1 (MQ=255) gAAGCTGTATCACCGGCATTTTTTGCTGCTGCTAAGAGTGAGGTATAACAGGCGATTTCAAATTGCTCg < 1:2239260/69‑1 (MQ=255) gAAGCTGTATCACCGGCATTTTTTGCTGCTGCTAAGAGTGAGGTATAACAGGCGATTTCAAATTGCTCg < 1:2507398/69‑1 (MQ=255) gAAGCTGTATCACCGGCATTTTTTGCTGCTGCTAAGAGTGAGGTATAACAGGCGATTTCAAATTGCTCg < 1:2019929/69‑1 (MQ=255) gAAGCTGTATCACCGGCATTTTTTGCTGCTGCTAAGAGTGAGGTATAACAGGCGATTTCAAATTGCTCg < 1:2600405/69‑1 (MQ=255) gAAGCTGTATCACCGGCATTTTTTGCTGCTGCTAAGAGTGAGGTATAACAGGCGATTTCAAATTGCTCg < 1:1584494/69‑1 (MQ=255) gAAGCTGTATCACCGGCATTTTTTGCTGCTGCTAAGAGTGAGGTATAACAGGCGATTTCAAATTGCTCg < 1:2993670/69‑1 (MQ=255) cTGTATCACCGGCATTTTTTGCTGCTGCTAATAGTGAGGTGTAACAGGCGATTTCAAATTGCTCGAAGa < 1:2760368/69‑1 (MQ=255) cTGTATCACCGGCATTTTTTGCTGCTGCTAATAGTGAGGTGTAACAGGCGATTTCAAATTGCTCGAAGa < 1:2654387/69‑1 (MQ=255) cTGTATCACCGGCATTTTTTGCTGCTGCTAATAGTGAGGTGTAACAGGCGATTTCAAATTGCTCGAAGa < 1:786042/69‑1 (MQ=255) aCCGGCATTTTTTGCTGCTGCTAATAGTGAGGTGTAACAGGCGATTTCa < 1:29113/49‑1 (MQ=255) aCCGGCATTTTTTGCTGCTGCTAATAGTGAGGTGTAACAGGCGATTTCa < 1:3167442/49‑1 (MQ=255) aCCGGCATTTTTTGCTGCTGCTAATAGTGAGGTGTAACAGGCGATTTCa < 1:11809/49‑1 (MQ=255) aCCGGCATTTTTTGCTGCTGCTAATAGTGAGGTGTAACAGGCGATTTCa < 1:2869861/49‑1 (MQ=255) aCCGGCATTTTTTGCTGCTGCTAATAGTGAGGTGTAACAGGCGATTTCa < 1:3347747/49‑1 (MQ=255) aCCGGCATTTTTTGCTGCTGCTAATAGTGAGGTGTAACAGGCGATTTCa < 1:689190/49‑1 (MQ=255) aCCGGCATTTTTTGCTGCTGCTAATAGTGAGGTGTAACAGGCGATTTCa < 1:2535604/49‑1 (MQ=255) aCCGGCATTTTTTGCTGCTGCTAATAGTGAGGTGTAACAGGCGATTTCa < 1:2331079/49‑1 (MQ=255) aCCGGCATTTTTTGCTGCTGCTAATAGTGAGGTGTAACAGGCGATTTCa < 1:796237/49‑1 (MQ=255) aCCGGCATTTTTTGCTGCTGCTAATAGTGAGGTGTAACAGGCGATTTCa < 1:2149371/49‑1 (MQ=255) aCCGGCATTTTTTGCTGCTGCTAATAGTGAGGTGTAACAGGCGATTTCa < 1:2147950/49‑1 (MQ=255) aCCGGCATTTTTTGCTGCTGCTAATAGTGAGGTGTAACAGGCGATTTCa < 1:1212730/49‑1 (MQ=255) ggCATTTTTTGCTGCTGCTAATAGTGAGGTGTAACAGGCGATTTCAAATTGCTCGAAGACATATCCGCt < 1:240417/69‑1 (MQ=255) gctAATAGTGAGGTGTAACAGGCGATTTCAAATTGCTCGAAGACATATCCGCTAATAGAGCCTTTGAc < 1:2208417/68‑1 (MQ=255) gctAATAGTGAGGTGTAACAGGCGATTTCAAATTGCTCGAAGACATATCCGCTAATAGAGCCTTTGAc < 1:1813943/68‑1 (MQ=255) gctAATAGTGAGGTGTAACAGGCGATTTCAAATTGCTCGAAGACATATCCGCTAATAGAGCCTTTGAc < 1:849063/68‑1 (MQ=255) gctAATAGTGAGGTGTAACAGGCGATTTCAAATTGCTCGAAGACATATCCGCTAATAGAGCCTTTGAc < 1:3250865/68‑1 (MQ=255) gctAATAGTGAGGTGTAACAGGCGATTTCAAATTGCTCGAAGACATATCCGCTAATAGAGCCTTTGAc < 1:2466049/68‑1 (MQ=255) gctAATAGTGAGGTGTAACAGGCGATTTCAAATTGCTCGAAGACATATCCGCTAATAGAGCCTTTGAc < 1:2625625/68‑1 (MQ=255) ctAATAGTGAGGTGTTACAGGCGATTTCAAATTGCTCGAAGACATATCCTCTAATAGAGCCTTTGAc < 1:1389485/67‑1 (MQ=255) aaTAGTGAGGTGTAACAGGCGATTTCAAATTGCTCGAAGACATATCCGCTAATAGAGCCTTTGACTAt < 1:598796/68‑1 (MQ=255) aTAGTGAGGTGTAACAGGCGATTTCAAATTGCTCGAAGACATATCCGCTAATAGAGCCTTTGACTAttt > 1:699513/1‑69 (MQ=255) gTGAGGTGTAACAGGCGATTTCAAATTGCTCGAAGAc > 1:1945522/1‑37 (MQ=255) gTGAGGTGTAACAGGCGATTTCAAATTGCTCGAAGACa > 1:791438/1‑38 (MQ=255) gTGAGGTGTAACAGGCGATTTCAAATTGCTCGAAGACa > 1:2189499/1‑38 (MQ=255) gTGAGGTGTAACAGGCGATTTCAAATTGCTCGAAGACa > 1:1248540/1‑38 (MQ=255) gTGAGGTGTAACAGGCGATTTCAAATTGCTCGAAGACa > 1:926025/1‑38 (MQ=255) gTGAGGTGTAACAGGCGATTTCAAATTGCTCGAAGACa > 1:2829477/1‑38 (MQ=255) gTGAGGTGTAACAGGCGATTTCAAATTGCTCGAAGACa > 1:2214310/1‑38 (MQ=255) gTGAGGTGTAACAGGCGATTTCAAATTGCTCGAAGACa > 1:2310188/1‑38 (MQ=255) gTGAGGTGTAACAGGCGATTTCAAATTGCTCGAAGACATATCCGCTAATAGAGCCTTTGACTATTtc > 1:1681501/1‑67 (MQ=255) gTGAGGTGTAACAGGCGATTTCAAATTGCTCGAAGACATATCCGCTAATAGAGCCTTTGACTATTtc > 1:577147/1‑67 (MQ=255) aGGTGTAACAGGCGATTTCAAATTGCTCGAAGACATATCCGCTAATAGAGCCTTTGACTATTTCATCAg < 1:221335/69‑1 (MQ=255) aGGTGTAACAGGCGATTTCAAATTGCTCGAAGACATATCCGCTAATAGAGCCTTTGACTATTTCATCAg < 1:1760681/69‑1 (MQ=255) aGGTGTAACAGGCGATTTCAAATTGCTCGAAGACATATCCGCTAATAGAGCCTTTGACTATTTCATCAg < 1:1245478/69‑1 (MQ=255) aGGTGTAACAGGCGATTTCAAATTGCTCGAAGACATATCCGCTAATAGAGCCTTTGACTATTTCATCAg < 1:2845117/69‑1 (MQ=255) aGGTGTAACAGGCGATTTCAAATTGCTCGAAGACATATCCGCTAATAGAGCCTTTGACTATTTCATCAg < 1:2114892/69‑1 (MQ=255) aGGTATAACAGGCGATTTCAAATTGCTCGAAGCCATATCCGCTAATAGAGCCTTTGACTATTtcat > 1:641010/1‑66 (MQ=255) aGGTATAACAGGCGATTTCAAATTGCTCGAAGACa > 1:2641665/1‑35 (MQ=255) aGGTATAACAGGCGATTTCAAATTGCTCGAAGACATATCCGCTAATAGAGCCTTTGACTATTtcatc > 1:2551532/1‑67 (MQ=255) aGGTATAACAGGCGATTTCAAATTGCTCGAAGACATATCCGCTAATAGAGCCTTTGACTATTtcatc > 1:877036/1‑67 (MQ=255) aGGTATAACAGGCGATTTCAAATTGCTCGAAGACATATCCGCTAATAGAGCCTTTGACTATTtcatc > 1:1040551/1‑67 (MQ=255) aGGTATAACAGGCGATTTCAAATTGCTCGAAGACATATCCGCTAATAGAGCCTTTGACTATTtcatc > 1:1196285/1‑67 (MQ=255) aGGTATAACAGGCGATTTCAAATTGCTCGAAGACATATCCGCTAATAGAGCCTTTGACTATTtcatc > 1:2069004/1‑67 (MQ=255) aGGTATAACAGGCGATTTCAAATTGCTCGAAGACATATCCGCTAATAGAGCCTTTGACTATTtcatc > 1:3413324/1‑67 (MQ=255) aGGTATAACAGGCGATTTCAAATTGCTCGAAGACATATCCGCTAATAGAGCCTTTGACTATTtcatc > 1:1625576/1‑67 (MQ=255) aGGTATAACAGGCGATTTCAAATTGCTCGAAGACATATCCGCTAATAGAGCCTTTGACTATTtcatc > 1:1697336/1‑67 (MQ=255) | ATCGCTTCGATGGTTGGAATTGAAGCTGTATCACCGGCATTTTTTGCTGCTGCTAATAGTGAGGTGTAACAGGCGATTTCAAATTGCTCGAAGACATATCCGCTAATAGAGCCTTTGACTATTTCATCAG > minE/853781‑853910 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |