| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 856,644 | A→T | 56.9% | A242A (GCT→GCA) | trpB ← | tryptophan synthase, beta subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 856,644 | 0 | A | T | 56.9% | 22.3 / 66.6 | 65 | A242A (GCT→GCA) | trpB | tryptophan synthase, beta subunit |
| Reads supporting (aligned to +/- strand): ref base A (5/23); new base T (19/18); total (24/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.90e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01 | |||||||||||
ACCGTGACCACCTGGCTCCACACCAATCAGGCCGACGTTGGTTTCATTGATGAAATCAGCAAACATGCCGATGGCATTCGAACCGCCGCCAACACAGGCGATAACGGCATCCGGCAGGCG > minE/856587‑856706 | aCCGTGACCACCTGGCTCCACACCAATCAGGCCGACGTTGGTTTCATTGATTAAATCAGCAAACATGcc < 1:1833646/69‑1 (MQ=255)aCCGTGACCACCTGGCTCCACACCAATCAGGCCGACGTTGGTTTCATTGATGAAATCAGCAAACATGcc > 1:1377666/1‑69 (MQ=255)aCCGTGACCACCTGGCTCCACACCAATCAGGCCGACGTTGGTTTCATTGATGAAATCAGCAAACATGcc > 1:715721/1‑69 (MQ=255)aCCGTGACCACCTGGCTCCACACCAATCAGGCCGACGTTGGTTTCATTGATGAAATCAGCAAACATGcc > 1:3275916/1‑69 (MQ=255) gACCACCTGGCTCCACACCAATCAGGCCGACGTTGGTTTCATTGATGAAATCAGCAAACATGCCGATgg < 1:3001422/69‑1 (MQ=255) gACCACCTGGCTCCACACCAATCAGGCCGACGTTGGTTTCATTGATGAAATCAGCAAACATGCCGATgg < 1:240635/69‑1 (MQ=255) gACCACCTGGCTCCACACCAATCAGGCCGACGTTGGTTTCATTGATGAAATCAGCAAACATGCCGATgg < 1:2060699/69‑1 (MQ=255) accaccTGGCTCCACACCAATCAGGCCGACGTTGGTTTCATTGATGAAATCAGCAAACATGCCGATGGc > 1:3254878/1‑69 (MQ=255) ccaccTGGCTCCACACCAATCAGGCCGACGTTGGTTTCATTGATGAAATCAGCAAACATGcc < 1:2331595/62‑1 (MQ=255) cacaCCAATCAGGCCGACGTCGGTTTCGTTGATGAAATCTGCAAAc > 1:2709059/1‑46 (MQ=255) cacaCCAATCAGGCCGACGTCGGTTTCGTTGATGAAATCTGCAAACATACCGATTGCATTCGAaccacc > 1:534816/1‑69 (MQ=255) cacaCCAATCAGGCCGACGTCGGTTTCGTTGATGAAATCTGCAAACATACCGATGGCATTCGAaccacc > 1:2719830/1‑69 (MQ=255) cacaCCAATCAGGCCGACGTCGGTTTCGTTGATGAAATCTGCAAACATACCGATGGCATTCGAaccacc > 1:2380946/1‑69 (MQ=255) cacaCCAATCAGGCCGACGTCGGTTTCGTTGATGAAATCTGCAAACATACCGATGGCATTCGAaccacc > 1:3331432/1‑69 (MQ=255) cacaCCAATCAGGCCGACGTCGGTTTCGTTGATGAAATCTGCAAACATACCGATGGCATTCGAaccacc > 1:3307522/1‑69 (MQ=255) cacaCCAATCAGGCCGACGTCGGTTTCGTTGATGAAATCTGCAAACATACCGATGGCATTCGAaccacc > 1:419462/1‑69 (MQ=255) cacaCCAATCAGGCCGACGTCGGTTTCGTTGATGAAATCTGCAAACATACCGATGGCATTCGAaccacc > 1:499496/1‑69 (MQ=255) cacaCCAATCAGGCCGACGTCGGTTTCGTTGATGAAATCTGCAAACATACCGATGGCATTCGAaccacc > 1:592521/1‑69 (MQ=255) cacaCCAATCAGGCCGACGTCGGTTTCGTTGATGAAATCTGCAAACATACCGATGGCATTCGAaccacc > 1:3283702/1‑69 (MQ=255) cacaCCAATCAGGCCGACGTCGGTTTCGTTGATGAAATCTGCAAACATACCGATGGCATTCGAaccacc > 1:1527345/1‑69 (MQ=255) cacaCCAATCAGGCCGACGTCGGTTTCGTTGATGAAATCTGCAAACATACCGATGGCATTCGAaccacc > 1:2976422/1‑69 (MQ=255) cacaCCAATCAGGCCGACGTCGGTTTCGTTGATGAAATCTGCAAACATACCGATGGCATTCGAaccacc > 1:8880/1‑69 (MQ=255) cacaCCAATCAGGCCGACGTCGGTTTCGTTGATGAAATCTGCAAACATACCGATGGCATTCGAaccacc > 1:1387417/1‑69 (MQ=255) cacaCCAATCAGGCCGACGTCGGTTTCGTTGATGAAATCTGCAAACATACCGATGGCATTCGAaccacc > 1:1308298/1‑69 (MQ=255) cacaCCAATCAGGCCGACGTCGGTTTCGTTGATGAAATCTGCAAACATACCGATGGCATTCGAaccacc > 1:1292320/1‑69 (MQ=255) cacaCCAATCAGGCCGACGTCGGTTTCGTTGATGAAATCTGCAAACATACCGATGGCATTCGAaccacc > 1:1248313/1‑69 (MQ=255) cacaCCAATCAGGCCGACGTCGGTTTCGTTGATGAAATCTGCAAACATACCGATGGCATTCGAAccac > 1:303790/1‑66 (MQ=255) cacaCCAATCAGGCCGACGTCGGTTTCGTTGATGAAATCTGAAAACATACCGATGGCATTCGAaccacc > 1:995339/1‑69 (MQ=255) cAATCAGGCCGACGTTGGTTTCATTGATGAAATCAGCAAACATGCCGATGGCa < 1:1549075/53‑1 (MQ=255) cAATCAGGCCGACGTTGGTTTCATTGATGAAATCAGCAAACATGCCGATGGCa < 1:849728/53‑1 (MQ=255) cAATCAGGCCGACGTTGGTTTCATTGATGAAATCAGCAAACATGCCGATGGCATTCGAACCGCCGCCaa < 1:3397863/69‑1 (MQ=255) cAATCAGGCCGACGTTGGTTTCATTGATGAAATCAGCAAACATGCCGATGGCATTCGAACCGCCGCCaa < 1:615313/69‑1 (MQ=255) cAATCAGGCCGACGTTGGTTTCATTGATGAAATCAGCAAACATGCCGATGGCATTCGAACCGCCGCCaa < 1:694425/69‑1 (MQ=255) cAATCAGGCCGACGTTGGTTTCATTGATGAAATCAGCAAACATGCCGATGGCATTCGAACCGCCGCCaa < 1:3140085/69‑1 (MQ=255) cAATCAGGCCGACGTTGGTTTCATTGATGAAATCAGCAAACATGCCGATGGCATTCGAACCGCCGCCaa < 1:297942/69‑1 (MQ=255) cAATCAGGCCGACGTTGGTTTCATTGATGAAATCAGCAAACATGCCGATGGCATTCGAACCGCCGCCaa < 1:1156030/69‑1 (MQ=255) cAATCAGGCCGACGTTGGTTTCATTGATGAAATCAGCAAACATGCCGATGGCATTCGAACCGCCGCCaa < 1:3010050/69‑1 (MQ=255) cAATCAGGCCGACGTTGGTTTCATTGATGAAATCAGCAAACATGCCGATGGCATTCGAACCGCCGCCaa < 1:2976296/69‑1 (MQ=255) cAATCAGGCCGACGTTGGTTTCATTGATGAAATCAGCAAACATGCCGATGGCATTCGAACCGCCGCCaa < 1:1617030/69‑1 (MQ=255) cAATCAGGCCGACGTTGGTTTCATTGATGAAATCAGCAAACATGCCGATGGCATTCGAACCGCCGCCaa < 1:1598035/69‑1 (MQ=255) aaTCAGGCCGACGTTGGTTTCATTGATGAAATCAGCAAACATGCCGATGGCATTCGAACCGCCGCCaa < 1:2824501/68‑1 (MQ=255) aaTCAGGCCGACGTTGGTTTCATTGATGAAATCAGCAAACATGCCGATGGCATTCGAACCGCCGCCaa < 1:1714576/68‑1 (MQ=255) ggTTTCATTGATGAAATCAGCAAACATGCCGATGGCATTCGAACCGCCGCCAACACAGGCGATAACGGc > 1:552548/1‑69 (MQ=255) ttCATTGATGAAATCAGCAAACATGCCGATGGCATTCGAACCGCCGCCAACACAGGCGATAACGGCa < 1:1881459/67‑1 (MQ=255) tgatgaAATCTGCAAACATACCGATGGCATTCGAACCACCGCCAACACAGGCGATAACGGCATCCGGc < 1:1633354/68‑1 (MQ=255) tgatgaAATCTGCAAACATACCGATGGCATTCGAACCACCGCCAACACAGGCGATAACGGCATCCGGc < 1:1161347/68‑1 (MQ=255) tgatgaAATCTGCAAACATACCGATGGCATTCGAACCACCGCCAACACAGGCGATAACGGCATCCGGc < 1:656838/68‑1 (MQ=255) tgatgaAATCTGCAAACATACCGATGGCATTCGAACCACCGCCAACACAGGCGATAACGGCATCCGGc < 1:1503790/68‑1 (MQ=255) tgatgaAATCTGCAAACATACCGATGGCATTCGAACCACCGCCAACACAGGCGATAACGGCATCCGGc < 1:732202/68‑1 (MQ=255) tgatgaAATCTGCAAACATACCGATGGCATTCGAACCACCGCCAACACAGGCGATAACGGCATCCGGc < 1:832166/68‑1 (MQ=255) tgatgaAATCTGCAAACATACCGATGGCATTCGAACCACCGCCAACACAGGCGATAACGGCATCCGGc < 1:1453933/68‑1 (MQ=255) tgatgaAATCTGCAAACATACCGATGGCATTCGAACCACCGCCAACACAGGCGATAACGGCATCCGGc < 1:889188/68‑1 (MQ=255) tgatgaAATCTGCAAACATACCGATGGCATTCGAACCACCGCCAACACAGGCGATAACGGCATCCGGc < 1:941219/68‑1 (MQ=255) tgatgaAATCTGCAAACATACCGATGGCATTCGAACCACCGCCAACACAGGCGATAACGGCATCCGGc < 1:586174/68‑1 (MQ=255) tgatgaAATCTGCAAACATACCGATGGCATTCGAACCACCGCCAACACAGGCGATAACGGCATCCGGc < 1:168317/68‑1 (MQ=255) tgatgaAATCTGCAAACATACCGATGGCATTCGAACCACCGCCAACACAGGCGATAACGGCATCCGGc < 1:451216/68‑1 (MQ=255) tgatgaAATCTGCAAACATACCGATGGCATTCGAACCACCGCCAACACAGGCGATAACGGCATCCGGc < 1:431042/68‑1 (MQ=255) tgatgaAATCTGCAAACATACCGATGGCATTCGAACCACCGCCAACACAGGCGATAACGGCATCCGGc < 1:2123353/68‑1 (MQ=255) tgatgaAATCTGCAAACATACCGATGGCATTCGAACCACCGCCAACACAGGCGATAACGGCATCCGGc < 1:2761032/68‑1 (MQ=255) tgatgaAATCTGCAAACATACCGATGGCATTCGAACCACCGCCAACACAGGCGATAACGGCATCCGGc < 1:2975899/68‑1 (MQ=255) tgatgaAATCTGCAAACATACCGATGGCATTCGAACCACCGCCAACACAGGCGATAACGGCATCCGGc < 1:3274273/68‑1 (MQ=255) tgatgaAATCTGCAAACATACCGATGGCATTCGAACCACCGCCAACACAGGCGATAACGGCATCCGGc < 1:3225144/68‑1 (MQ=255) gaAATCAGCAAACATGCCGATGGCATTCGAACCGCCGCCAACACAGGCGATAACGGCATCCGTCAGGCg < 1:436658/69‑1 (MQ=255) gaAATCAGCAAACATGCCGATGGCATTCGAACCGCCGCCAACACAGGCGATAACGGCATCCGGCAGGCg < 1:2302695/69‑1 (MQ=255) gaAATCAGCAAACATGCCGATGGCATTCGAACCGCCGCCAACACAGGCGATAACGGCATCCGGCAGGCg < 1:1463463/69‑1 (MQ=255) | ACCGTGACCACCTGGCTCCACACCAATCAGGCCGACGTTGGTTTCATTGATGAAATCAGCAAACATGCCGATGGCATTCGAACCGCCGCCAACACAGGCGATAACGGCATCCGGCAGGCG > minE/856587‑856706 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |