| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 856,734 | G→C | 32.6% | T212T (ACC→ACG) | trpB ← | tryptophan synthase, beta subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 856,734 | 0 | G | C | 32.6% | 104.9 / 72.5 | 98 | T212T (ACC→ACG) | trpB | tryptophan synthase, beta subunit |
| Reads supporting (aligned to +/- strand): ref base G (22/44); new base C (5/27); total (27/71) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.14e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.54e-01 | |||||||||||
GCCGCCAACACAGGCGATAACGGCATCCGGCAGGCGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCGGATAAGGATGCGGGCCAGC > minE/856671‑856796 | gccgccAACACAGGCGATAACGGCATCCGGCAGGCGACCTTCTCTTTCCAGAATCTGCGCTTTGGTttc < 1:2305201/69‑1 (MQ=255)gccgccAACACAGGCGATAACGGCATCCGGCAGGCGACCTTCTCTTTCCAGAATCTGCGCTTTGGTttc < 1:1269287/69‑1 (MQ=255) cgccAACACAGGCGATAACGGCATCCGGCAGGCGACCTTCTCTTTCCAGAATCTGCGCTTTGGTttc < 1:3121241/67‑1 (MQ=255) cGATAACGGCATCCGGCAGGCGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAatcatc > 1:1657291/1‑68 (MQ=255) cGATAACGGCATCCGGCAGGCGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATcc > 1:1110794/1‑69 (MQ=255) cGATAACGGCATCCGGCAGGCGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATcc > 1:2903473/1‑69 (MQ=255) cGATAACGGCATCCGGCAGGCGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATcc > 1:2417268/1‑69 (MQ=255) cGATAACGGCATCCGGCAGGCGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATcc > 1:573694/1‑69 (MQ=255) cGATAACGGCATCCGGCAGGCGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATcc > 1:1418747/1‑69 (MQ=255) cGATAACGGCATCCGGCAGGCGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATcc > 1:1299299/1‑69 (MQ=255) aCGGCATCCGGCAGGCGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGa < 1:1180255/69‑1 (MQ=255) aCGGCATCCGGCAGGCGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGa < 1:2351667/69‑1 (MQ=255) aCGGCATCCGGCAGGCGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGa < 1:2582494/69‑1 (MQ=255) aCGGCATCCGGCAGGCGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGa < 1:2742906/69‑1 (MQ=255) aCGGCATCCGGCAGGCGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGa < 1:3188511/69‑1 (MQ=255) aCGGCATCCGGCAGGCGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGa < 1:774855/69‑1 (MQ=255) aCGGCATCCGGCAGGCGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGa < 1:1293873/69‑1 (MQ=255) gCATCCGGCAGGCGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAAc < 1:3047319/69‑1 (MQ=255) ccGGCAGGCGACCTTCTCTTTCCAGAATCTGCGCTTTCGt > 1:3158515/1‑40 (MQ=255) ccGGCAGGCGACCTTCTCTTTCCAGAATCTGCGCTTTCGTTTCTTCGCCAATCATCCGCTGAAACTCa > 1:3053034/1‑68 (MQ=255) ccGGCAGGCGACCTTCTCTTTCCAGAATCTGCGCTTTCGTTTCTTCGCCAATCATCCGCTGAAACTCa > 1:3105703/1‑68 (MQ=255) ccGGCAGGCGACCTTCTCTTTCCAGAATCTGCGCTTTCGTTTCTTCGCCAATCATCCGCTGAAACTCa > 1:2419289/1‑68 (MQ=255) ccGGCAGGCGACCTTCTATTTCCAGAATCTGCGCTTTCGTttct > 1:1161683/1‑44 (MQ=38) cGGCAGGCGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACg < 1:837926/69‑1 (MQ=255) aGGCGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGcaca < 1:1609575/69‑1 (MQ=255) aGGCGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGcaca < 1:2376479/69‑1 (MQ=255) aGGCGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGcaca < 1:396190/69‑1 (MQ=255) aGGCGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGcaca < 1:1917522/69‑1 (MQ=255) aGGCGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGcaca < 1:3219491/69‑1 (MQ=255) aGGCGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGcaca < 1:1468422/69‑1 (MQ=255) cGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACt < 1:3262423/58‑1 (MQ=255) cGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACt < 1:872425/58‑1 (MQ=255) cTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCg < 1:314400/69‑1 (MQ=255) cTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCg > 1:2869807/1‑69 (MQ=255) ctctTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCgg > 1:785355/1‑67 (MQ=255) ctctTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCgg > 1:1795412/1‑67 (MQ=255) ctctTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCgg > 1:2549761/1‑67 (MQ=255) ctctTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCgg > 1:3223489/1‑67 (MQ=255) ctctTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCgg > 1:3123649/1‑67 (MQ=255) ctctTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCgg > 1:669346/1‑67 (MQ=255) ctctTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCg > 1:611930/1‑66 (MQ=255) ctctTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCg > 1:3173014/1‑66 (MQ=255) ctTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCGGATa > 1:1026702/1‑68 (MQ=255) ctTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCGGATa > 1:354164/1‑68 (MQ=255) ctTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCGGATa > 1:32825/1‑68 (MQ=255) ctTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCGGATa > 1:2547592/1‑68 (MQ=255) ctTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCGGATa > 1:121515/1‑68 (MQ=255) ctTTCCAGAATCTGCGCTTTCGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACaa < 1:687834/58‑1 (MQ=255) ctTTCCAGAATCTGCGCTTTCGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACaa < 1:668135/58‑1 (MQ=255) ctTTCCAGAATCTGCGCTTTCGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACaa < 1:717710/58‑1 (MQ=255) ctTTCCAGAATCTGCGCTTTCGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACaa < 1:957599/58‑1 (MQ=255) ctTTCCAGAATCTGCGCTTTCGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACaa < 1:314055/58‑1 (MQ=255) ctTTCCAGAATCTGCGCTTTCGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACaa < 1:3338803/58‑1 (MQ=255) ctTTCCAGAATCTGCGCTTTCGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACaa < 1:898347/58‑1 (MQ=255) ctTTCCAGAATCTGCGCTTTCGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACaa < 1:498831/58‑1 (MQ=255) ctTTCCAGAATCTGCGCTTTCGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACaa < 1:457583/58‑1 (MQ=255) ctTTCCAGAATCTGCGCTTTCGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACaa < 1:3361122/58‑1 (MQ=255) ctTTCCAGAATCTGCGCTTTCGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACaa < 1:14691/58‑1 (MQ=255) ctTTCCAGAATCTGCGCTTTCGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACaa < 1:1801552/58‑1 (MQ=255) ctTTCCAGAATCTGCGCTTTCGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACaa < 1:3006082/58‑1 (MQ=255) ctTTCCAGAATCTGCGCTTTCGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACaa < 1:1618605/58‑1 (MQ=255) ctTTCCAGAATCTGCGCTTTCGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACaa < 1:1617496/58‑1 (MQ=255) ctTTCCAGAATCTGCGCTTTCGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACaa < 1:2511593/58‑1 (MQ=255) ctTTCCAGAATCTGCGCTTTCGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACaa < 1:2524222/58‑1 (MQ=255) ctTTCCAGAATCTGCGCTTTCGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACaa < 1:1468057/58‑1 (MQ=255) ctTTCCAGAATCTGCGCTTTCGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACaa < 1:2606030/58‑1 (MQ=255) ctTTCCAGAATCTGCGCTTTCGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACaa < 1:1389541/58‑1 (MQ=255) ctTTCCAGAATCTGCGCTTTCGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACaa < 1:2768263/58‑1 (MQ=255) ctTTCCAGAATCTGCGCTTTCGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACaa < 1:2785519/58‑1 (MQ=255) ctTTCCAGAATCTGCGCTTTCGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACaa < 1:1182423/58‑1 (MQ=255) ctTTCCAGAATCTGCGCTTTCGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACaa < 1:2880203/58‑1 (MQ=255) ctTTCCAGAATCTGCGCTTTCGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACaa < 1:2953942/58‑1 (MQ=255) ctTTCCAGAATCTGCCCTTTCGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACaa < 1:410019/58‑1 (MQ=255) tttCCAGAATCTGCGCTTTCGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACaa < 1:3141090/57‑1 (MQ=255) tCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCGGATAAg < 1:428405/67‑1 (MQ=255) tCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCGGATAAg < 1:734811/67‑1 (MQ=255) tCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCGGATAAg < 1:1622674/67‑1 (MQ=255) tCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCGGATAAg < 1:1670819/67‑1 (MQ=255) tCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCGGATAAg < 1:666592/67‑1 (MQ=255) tCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCGGATAAg < 1:1944067/67‑1 (MQ=255) tCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCGGATAAg < 1:2788297/67‑1 (MQ=255) tCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCGGATAAg < 1:2726247/67‑1 (MQ=255) tCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCGGATAAg < 1:3259529/67‑1 (MQ=255) tCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCGGATAAg < 1:3272845/67‑1 (MQ=255) tCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCGGATAAg < 1:2199364/67‑1 (MQ=255) tCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCGGATAAg < 1:3393929/67‑1 (MQ=255) tCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCGGATAAg < 1:2101824/67‑1 (MQ=255) tCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCGGATAAg < 1:459865/67‑1 (MQ=255) aTCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACt < 1:3275166/40‑1 (MQ=255) aTCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCg > 1:1420537/1‑55 (MQ=255) cTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCGGTTAAGGATGCGGGcc < 1:2167632/69‑1 (MQ=255) cTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCGGATAAGGATGCGGGcc < 1:642825/69‑1 (MQ=255) cTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCGGATAAGGATGCGGGcc < 1:1765625/69‑1 (MQ=255) cTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCGGATAAGGATGCGGGcc < 1:597885/69‑1 (MQ=255) cTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCGGATAAGGATGCGGGcc < 1:1769587/69‑1 (MQ=255) cTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCGGATAAGGATGCGGGcc < 1:511705/69‑1 (MQ=255) cTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCGGATAAGGATGCGGGcc < 1:1128569/69‑1 (MQ=255) cTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCGGATAAGGATGCGGGCCAGc < 1:2262576/67‑1 (MQ=255) | GCCGCCAACACAGGCGATAACGGCATCCGGCAGGCGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCGGATAAGGATGCGGGCCAGC > minE/856671‑856796 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |