| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 857,834 | C→T | 54.8% | A302A (GCG→GCA) | trpC ← | fused indole‑3‑glycerolphosphate synthetase and N‑(5‑phosphoribosyl)anthranilate isomerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 857,834 | 0 | C | T | 54.8% | 23.1 / 115.7 | 137 | A302A (GCG→GCA) | trpC | fused indole‑3‑glycerolphosphate synthetase and N‑(5‑phosphoribosyl)anthranilate isomerase |
| Reads supporting (aligned to +/- strand): ref base C (24/38); new base T (26/49); total (50/87) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.22e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01 | |||||||||||
Only 100 of 152 total aligned reads displayed.
AGCTTTGTCCACCACATCGGCAATATCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCCGCAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGTGATGTCGCAACAAAAATCAA > minE/857771‑857899 | cTTTGTCCACCACATCGGCAATATCTTGATTGCGGAACACGCCAACATACTGCAACGGTGCCGCAGc < 1:1604625/67‑1 (MQ=255) cTTTGTCCACCACATCGGCAATATCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCCGCAGc < 1:546709/67‑1 (MQ=255) cTTTGTCCACCACATCGGCAATATCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCCGCAGc < 1:713457/67‑1 (MQ=255) cTTTGTCCACCACATCGGCAATATCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCCGCAGc < 1:2188430/67‑1 (MQ=255) cTTTGTCCACCACATCGGCAATATCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCCGCAGc < 1:804682/67‑1 (MQ=255) cTTTGTCCACCACATCGGCAATATCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCCGCAGc < 1:931505/67‑1 (MQ=255) tttGTCCACCACATCGGCAATATCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCCGCAGCCAt < 1:1298419/69‑1 (MQ=255) tttGTCCACCACATCGGCAATATCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCCGCAGCCAt < 1:3070401/69‑1 (MQ=255) tttGTCCACCACATCGGCAATATCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCCGCAGCCAt < 1:1269569/69‑1 (MQ=255) tttGTCCACCACATCGGCAATATCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCCGCAGCCAt < 1:3282746/69‑1 (MQ=255) tttGTCCACCACATCGGCAATATCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCCGCAGCCAt < 1:2113108/69‑1 (MQ=255) acaTCGGCAATATCGTGATTGCGGACCACGCCAACATACTGCAACGGTGCCGCAGCCATCACTTcc > 1:2245667/1‑66 (MQ=255) acaTCGGCAATATCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCCGCAGCCATCACTTcc > 1:2336075/1‑66 (MQ=255) acaTCGGCAATATCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCCGCAGCCATCACTTcc > 1:1814657/1‑66 (MQ=255) acaTCGGCAATATCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCCGCAGCCATCACTTcc > 1:61687/1‑66 (MQ=255) acaTCGGCAATATCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCCGCAGCCATCACTTcc > 1:3191736/1‑66 (MQ=255) acaTCGGCAATATCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCCGCAGCCATCACTTcc > 1:2944613/1‑66 (MQ=255) acaTCGGCAATATCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCCGCAGCCATCACTTcc > 1:2816334/1‑66 (MQ=255) acaTCGGCAATATCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCCGCAGCCATCACTTcc > 1:776492/1‑66 (MQ=255) acaTCGGCAATATCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCCGCAGCCATCACTTcc > 1:2676570/1‑66 (MQ=255) acaTCGGCAATATCGTGATTGCGGAACACGCCAACATACTGCAACGGTGACGCAGCCATCACTTcc > 1:1428927/1‑66 (MQ=255) ggCAATATCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTg < 1:2873141/69‑1 (MQ=255) ggCAATATCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTg < 1:322203/69‑1 (MQ=255) ggCAATATCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTg < 1:2422192/69‑1 (MQ=255) ggCAATATCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTg < 1:2452429/69‑1 (MQ=255) ggCAATATCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTg < 1:2193495/69‑1 (MQ=255) ggCAATATCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTg < 1:275759/69‑1 (MQ=255) ggCAATATCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTg < 1:1004328/69‑1 (MQ=255) ggCAATATCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTg < 1:3107858/69‑1 (MQ=255) ggCAATATCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTg < 1:2563850/69‑1 (MQ=255) ggCAATATCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTg < 1:2082948/69‑1 (MQ=255) ggCAATATCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTg < 1:1106198/69‑1 (MQ=255) ggCAATATCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTg < 1:3400687/69‑1 (MQ=255) ggCAATATCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTg < 1:116704/69‑1 (MQ=255) ggCAATATCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTg < 1:1856363/69‑1 (MQ=255) ggCAATATCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTg < 1:91251/69‑1 (MQ=255) ggCAATATCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTg < 1:1574255/69‑1 (MQ=255) ggCAATATCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTg < 1:673673/69‑1 (MQ=255) ggCAATATCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTg < 1:1681015/69‑1 (MQ=255) ggCAATATCGTGATTGCGGAACACGCCAACATACTGCAACGGTGACGCAGCCATCACTTCCTg > 1:2823837/1‑63 (MQ=255) gCAATATCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTg < 1:2712615/68‑1 (MQ=255) atCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCCGCAGCCATCACTTCCTg > 1:1071544/1‑57 (MQ=255) atCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCCGCAGCCATCACTTCCTGCGCCTGTTCAAc > 1:3157072/1‑69 (MQ=255) atCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCCGCAGCCATCACTTCCTGCGCCTGTTCAAc > 1:2762139/1‑69 (MQ=255) atCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCCGCAGCCATCACTTCCTGCGCCTGTTCAAc > 1:1548979/1‑69 (MQ=255) tCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTGTTCATCg < 1:2574370/69‑1 (MQ=255) tCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTGTTCAACg > 1:709664/1‑69 (MQ=255) tCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTGTTCAACg > 1:2920084/1‑69 (MQ=255) tCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTGTTCAACg > 1:729298/1‑69 (MQ=255) tCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTGTTCAACg > 1:773755/1‑69 (MQ=255) tCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTGTTCAACg < 1:2989097/69‑1 (MQ=255) tCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTGTTCAACg > 1:611366/1‑69 (MQ=255) tCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTGTTCAACg > 1:2722961/1‑69 (MQ=255) tCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTGTTCAACg < 1:3065026/69‑1 (MQ=255) tCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTGTTCAACg > 1:2511631/1‑69 (MQ=255) tCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTGTTCAACg > 1:2511046/1‑69 (MQ=255) tCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTGTTCAACg > 1:1842178/1‑69 (MQ=255) tCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTGTTCAACg < 1:1765363/69‑1 (MQ=255) tCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTGTTCAACg > 1:2356930/1‑69 (MQ=255) tCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTGTTCAACg > 1:230152/1‑69 (MQ=255) tCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTGTTCAACg > 1:2031812/1‑69 (MQ=255) tCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTGTTCAACg > 1:1633744/1‑69 (MQ=255) tCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTGTTCAACg < 1:2114667/69‑1 (MQ=255) tCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTGTTCAACg > 1:2155043/1‑69 (MQ=255) tCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTGTTCAACg > 1:2177000/1‑69 (MQ=255) tCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTGTTCAACg > 1:143454/1‑69 (MQ=255) tCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTGTTCAACg < 1:146420/69‑1 (MQ=255) tCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTGTTCAACg < 1:1621447/69‑1 (MQ=255) tCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCCGCAGCCATCACTTCCTGCGCCTGTTCAACg > 1:456201/1‑69 (MQ=255) tCGTGATTGCGGAACACGCCAACATACTGCAACGCTGCTGCAGCCATCACTTCCTGCGCCTGTTCAACg > 1:2664568/1‑69 (MQ=255) cGTGATTGCGGAACACGCCAACATACTGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTGTTCAACg < 1:3140070/68‑1 (MQ=255) cGTGATTGCGGAACACGCCAACATACTGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTGTTCAACg < 1:20048/68‑1 (MQ=255) cGTGATTGCGGAACACGCCAACATACTGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTGTTCAACg < 1:2404715/68‑1 (MQ=255) cGTGATTGCGGAACACGCCAACATAATGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTGTTCAACg < 1:1675715/68‑1 (MQ=255) gTGATTGCGGAACACGCCAACATACTGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTGTTCAACg < 1:654904/67‑1 (MQ=255) tGATTGCGGAACACGCCAACATACTGCAACGGTGCCGCAGCCATCACTTCCTGCGCCTGTTCAACGTTg > 1:97858/1‑69 (MQ=255) tGCGGAACACGCCAACATACTGCAACGGTGCCGCAGc < 1:3094118/37‑1 (MQ=255) aaCACGCCAACATACTGCAACGGTGCCGCAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAAcgc < 1:2225877/69‑1 (MQ=255) acGCCAACATACTGCAACGGTGCCGCAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGt < 1:870315/69‑1 (MQ=255) acGCCAACATACTGCAACGGTGCCGCAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGt < 1:289468/69‑1 (MQ=255) acGCCAACATACTGCAACGGTGCCGCAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGt < 1:2292488/69‑1 (MQ=255) acGCCAACATACTGCAACGGTGCCGCAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGt < 1:72491/69‑1 (MQ=255) acGCCAACATACTGCAACGGTGCCGCAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGt < 1:1929653/69‑1 (MQ=255) aTACTGCAACGGTGCCGCAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGTGATGTCGc < 1:1286722/69‑1 (MQ=255) aTACTGCAACGGTGCCGCAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGTGATGTCGc < 1:2590511/69‑1 (MQ=255) aTACTGCAACGGTGCCGCAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGTGATGTCGc < 1:3223841/69‑1 (MQ=255) aTACTGCAACGGTGCCGCAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGTGATGTCGc < 1:295697/69‑1 (MQ=255) cTGCAACGGTGCCGCAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGTGATGTCGcaa > 1:2452450/1‑68 (MQ=255) ggTGCCGCAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGTGa < 1:1781013/53‑1 (MQ=255) tgctgcATCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGTGATGTCGCAACAAAAATCaa > 1:2240213/1‑69 (MQ=255) tgctgcAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGTGATGTCGCAACAAAAATCaa > 1:3401765/1‑69 (MQ=255) tgctgcAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGTGATGTCGCAACAAAAATCaa > 1:2001170/1‑69 (MQ=255) tgctgcAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGTGATGTCGCAACAAAAATCaa > 1:3338632/1‑69 (MQ=255) tgctgcAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGTGATGTCGCAACAAAAATCaa > 1:1715015/1‑69 (MQ=255) tgctgcAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGTGATGTCGCAACAAAAATCaa > 1:2023894/1‑69 (MQ=255) tgctgcAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGTGATGTCGCAACAAAAATCaa > 1:2084567/1‑69 (MQ=255) tgctgcAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGTGATGTCGCAACAAAAATCaa > 1:3002372/1‑69 (MQ=255) tgctgcAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGTGATGTCGCAACAAAAATCaa > 1:2218289/1‑69 (MQ=255) tgctgcAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGTGATGTCGCAACAAAAATCaa > 1:2923645/1‑69 (MQ=255) tgctgcAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGTGATGTCGCAACAAAAATCa > 1:1334826/1‑68 (MQ=255) | AGCTTTGTCCACCACATCGGCAATATCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCCGCAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGTGATGTCGCAACAAAAATCAA > minE/857771‑857899 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |