| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 858,231 | T→A | 52.3% | Q170L (CAG→CTG) | trpC ← | fused indole‑3‑glycerolphosphate synthetase and N‑(5‑phosphoribosyl)anthranilate isomerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 858,231 | 0 | T | A | 52.3% | 8.1 / 101.6 | 86 | Q170L (CAG→CTG) | trpC | fused indole‑3‑glycerolphosphate synthetase and N‑(5‑phosphoribosyl)anthranilate isomerase |
| Reads supporting (aligned to +/- strand): ref base T (16/25); new base A (26/19); total (42/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.01e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCGACAAATCACGCAGATCGCGGTTGTTGATGCCAACGACCTTTGCTCCCAATGCAATGGCGCGCTCCTGTTCCTCTTCATTACTGACTTCGGTCAGCACCCCCATCTCCAGACTGTGAGCGACG > minE/858163‑858287 | tCGACAAATCACGCAGATCGCGGTTGTTGATGCCAACGACCTTTGCTCCCAATGCAATGGCGCGCTCCt > 1:519179/1‑69 (MQ=255)tCGACAAATCACGCAGATCGCGGTTGTTGATGCCAACGACCTTTGCTCCCAATGCAATGGCGCGCTCCt > 1:1695814/1‑69 (MQ=255)tCGACAAATCACGCAGATCGCGGTTGTTGATGCCAACGACCTTTGCTCCCAATGCAATGGCGCGCTCCt > 1:2305717/1‑69 (MQ=255)tCGACAAATCACGCAGATCGCGGTTGTTGATGCCAACGACCTTTGCTCCCAATGCAATGGCGCGCTCCt > 1:2302379/1‑69 (MQ=255)tCGACAAATCACGCAGATCGCGGTTGTTGATGCCAACGACCTTTGCTCCCAATGCAATGGCGCGCTCCt > 1:1189341/1‑69 (MQ=255)tCGACAAATCACGCAGATCGCGGTTGTTGATGCCAACGACCTTTGCTCCCAATGCAATGGCGCGCTCCt > 1:806499/1‑69 (MQ=255)tCGACAAATCACGCAGATCGCGGTTGTTGATGCCAACGACCTTTGCTCCCAATGCAATGGCGCGCTCCt > 1:1808778/1‑69 (MQ=255)tCGACAAATCACGCAGATCGCGGTTGTTGATGCCAACGACCTTTGATCCCAATGCAATGGCGCGCTCCt > 1:2846452/1‑69 (MQ=255) gACAAATCACGCAGATCGCGGTTGTTGATGCCAACGACCTTTGCTCCCAATGCAATGGCGCGCTCCTGt > 1:457611/1‑69 (MQ=255) gACAAATCACGCAGATCGCGGTTGTTGATGCCAACGACCTTTGCTCCCAATGCAATGGCGCGCTCCTGt > 1:3525/1‑69 (MQ=255) gACAAATCACGCAGATCGCGGTTGTTGATGCCAACGACCTTTGCTCCCAATGCAATGGCGCGCTCCTGt > 1:1469707/1‑69 (MQ=255) gACAAATCACGCAGATCGCGGTTGTTGATGCCAACGACCTTTGCTCCCAATGCAATGGCGCGCTCCTGt > 1:2835930/1‑69 (MQ=255) gACAAATCACGCAGATCGCGGTTGTTGATGCCAACGACCTTTGCTCCCAATGCAATGGCGCGCTCCTGt > 1:266330/1‑69 (MQ=255) aGATCGCGGTTGTTGATGCCAACGACCTTTGCTCCCAATGCAATGGCGCGCTCCTGTTCCTCTTCAtt < 1:371915/68‑1 (MQ=255) gATCGCGGTTGTTGATGCCAACGACCTTTGCTCCCAATGCAATGGCGCGCTCCTGTTCCTCTTCAtt < 1:1796099/67‑1 (MQ=255) gATGCCAACGACCTTTGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTCGGTCAg < 1:2821312/69‑1 (MQ=255) gATGCCAACGACCTTTGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTCGGTCAg < 1:2113935/69‑1 (MQ=255) gATGCCAACGACCTTTGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTCGGTCAg < 1:2259862/69‑1 (MQ=255) gATGCCAACGACCTTTGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTCGGTCAg < 1:1646828/69‑1 (MQ=255) gATGCCAACGACCTTTGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTCGGTCAg < 1:2374418/69‑1 (MQ=255) gATGCCAACGACCTTTGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTCGGTCAg < 1:250883/69‑1 (MQ=255) gATGCCAACGACCTTTGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTCGGTCAg < 1:1157826/69‑1 (MQ=255) gATGCCAACGACCTTTGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTCGGTCAg < 1:715819/69‑1 (MQ=255) gATGCCAACGACCTTTGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTCGGTCAg < 1:1482848/69‑1 (MQ=255) gATGCCAACGACCTTTGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTCGGTCAg < 1:1533434/69‑1 (MQ=255) gATGCCAACGACCTTTGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTCGGTCAg < 1:1064976/69‑1 (MQ=255) gATGCCAACGACCTTTGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTCGGTCAg < 1:868557/69‑1 (MQ=255) gATGCCAACGACCTTTGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTCGGTCAg < 1:81064/69‑1 (MQ=255) aTGCCAACGACCTTTGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTCGGTCAg < 1:1123342/68‑1 (MQ=255) aTGCCAACGACCTTTGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTCGGTCAg < 1:359519/68‑1 (MQ=255) aTGCCAACGACCTTTGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTCGGTCAg < 1:2576644/68‑1 (MQ=255) tGCCAACGACCTTTGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTCGGTCAg < 1:957271/67‑1 (MQ=255) gCCAACGACCTTTGCTCCCAATGCAATGGCGCGCTCCTGTTCCTCTTCATTACTGACTTCGGTCAGCAc < 1:1555325/69‑1 (MQ=255) cAACGACCTTTGCGCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTCg > 1:1068323/1‑59 (MQ=39) cAACGACCTTTGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACttg > 1:1779847/1‑57 (MQ=255) cAACGACCTTTGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACt > 1:1499989/1‑56 (MQ=255) cAACGACCTTTGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTCg > 1:985044/1‑59 (MQ=255) cAACGACCTTTGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTCg > 1:2732382/1‑59 (MQ=255) cAACGACCTTTGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTCg > 1:2451024/1‑59 (MQ=255) cAACGACCTTTGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTCg > 1:2741805/1‑59 (MQ=255) cAACGACCTTTGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTCg > 1:2768817/1‑59 (MQ=255) cAACGACCTTTGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTCg > 1:925355/1‑59 (MQ=255) cAACGACCTTTGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTCg > 1:915499/1‑59 (MQ=255) cAACGACCTTTGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTCg > 1:897796/1‑59 (MQ=255) cAACGACCTTTGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTCg > 1:3173391/1‑59 (MQ=255) cAACGACCTTTGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTCg > 1:3200215/1‑59 (MQ=255) cAACGACCTTTGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTCg > 1:3288823/1‑59 (MQ=255) cAACGACCTTTGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTCg > 1:726506/1‑59 (MQ=255) cAACGACCTTTGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTCg > 1:1385911/1‑59 (MQ=255) cAACGACCTTTGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTCg > 1:1695362/1‑59 (MQ=255) cAACGACCTTTGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTCg > 1:1698981/1‑59 (MQ=255) cAACGACCTTTGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTCg > 1:1554214/1‑59 (MQ=255) cAACGACCTTTGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTCg > 1:1488693/1‑59 (MQ=255) cAACGACCTTTGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTCg > 1:1447813/1‑59 (MQ=255) cAACGACCTTTGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTCg > 1:1831092/1‑59 (MQ=255) cAACGACCTTTGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTCg > 1:183521/1‑59 (MQ=255) cAACGACCTTTGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTCg > 1:1894808/1‑59 (MQ=255) cAACGACCTTTGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTCg > 1:1255990/1‑59 (MQ=255) cAACGACCTTTGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTCg > 1:2417948/1‑59 (MQ=255) gACCTTTGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTCGGTCAg < 1:2127528/60‑1 (MQ=255) tttGCTCCCAATGCAATGGCGCTCTCCTGTTCCTCTTCATTACTGACTTCGGTc < 1:1480614/54‑1 (MQ=255) tttGCTCCCAATGCAATGGCGCGCTCCTGTTCCTCTTCATTACTGACTTCGGTc < 1:2743916/54‑1 (MQ=255) tttGCTCCCAATGCAATGGCGCGCTCCTGTTCCTCTTCATTACTGACTTCGGTc < 1:877592/54‑1 (MQ=255) tttGCTCCCAATGCAATGGCGCGCTCCTGTTCCTCTTCATTACTGACTTCGGTc < 1:1384588/54‑1 (MQ=255) tttGCTCCCAATGCAATGGCGCGCTCCTGTTCCTCTTCATTACTGACTTCGGTc < 1:1771945/54‑1 (MQ=255) tttGCTCCCAATGCAATGGCGCGCTCCTGTTCCTCTTCATTACTGACTTCGGTc < 1:769924/54‑1 (MQ=255) tttGCTCCCAATGCAATGGCGCGCTCCTGTTCCTCTTCATTACTGACTTCGGTc < 1:560933/54‑1 (MQ=255) tttGCTCCCAATGCAATGGCGCGCTCCTGTTCCTCTTCATTACTGACTTCGGTc < 1:1095409/54‑1 (MQ=255) tttGCTCCCAATGCAATGGCGCGCTCCTGTTCCTCTTCATTACTGACTTCGGTc < 1:1079306/54‑1 (MQ=255) tctGCTCCCAATGCAATGGCGCGCTCCTGTTCCTCTTCATTACTGACTTCGGTc < 1:2575096/52‑1 (MQ=255) cTCCCAATGCAATGGCGCGCTCCTGTTCCTCTTCATTACTGACTTCGGTCAGCACCCCCATCTCCAg < 1:995933/67‑1 (MQ=255) cTCCCAATGCAATGGCGCGCTCCTGTTCCTCTTCATTACTGACTTCGGTCAGCACCCCCATCTCCAg < 1:1404102/67‑1 (MQ=255) cTCCCAATGCAATGGCGCGCTCCTGTTCCTCTTCATTACTGACTTCGGTCAGCACCCCCATCTCCAg < 1:104956/67‑1 (MQ=255) cTCCCAATGCAATGGCGCGCTCCTGTTCCTCTTCATTACTGACTTCGGTCAGCACCCCCATCTCCAg < 1:3150375/67‑1 (MQ=255) cTCCCAATGCAATGGCGCGCTCCTGTTCCTCTTCATTACTGACTTCGGTCAGCACCCCCATCTCCAg < 1:2510822/67‑1 (MQ=255) cTCCCAATGCAATGGCGCGCTCCTGTTCCTCTTCATTACTGACTTCGGTCAGCACCCCCATCTCCAg < 1:2598396/67‑1 (MQ=255) cTCCCAATGCAATGGCGCGCTCCTGTTCCTCTTCATTACTGACTTCGGTCAGCACCCCCATCTCCAg < 1:1876253/67‑1 (MQ=255) cTCCCAATGCAATGGCGCGCTCCTGTTCCTCTTCATTACTGACTTCGGTCAGCACCCCCATCTCCAg < 1:1869511/67‑1 (MQ=255) cTCCCAATGCAATGGCGCGCTCCTGTTCCTCTTCATTACTGACTTCGGTCAGCACCCCCATCTCCAg < 1:1744703/67‑1 (MQ=255) cTCCCAATGCAATGGCGCGCTCCTGTTCCTCTTCATTACTGACTTCGGTCAGCACCCCCATCTCCAg < 1:347233/67‑1 (MQ=255) cTCCCAATGCAATGGCGCGCTCCTGTTCCTCTTCATTACTGACTTCGGTCAGCACCCCCATCTACAg < 1:919370/67‑1 (MQ=255) aatgcaatgGCGCGCTCCTGTTCCTCTTCATTACTGACTTCGGTCAGCAc < 1:2328058/50‑1 (MQ=255) caatgGCGCGCTCCTGTTCCTCTTCATTACTGACTTCGGTCAGCACCCCCATCTCCAGACTGTGAGCGa > 1:501938/1‑69 (MQ=255) atgGCGCGCTCCTGTTCCTCTTCATTACTGACTTCGGTCAGCACCCCCATCTCCAGACTGTGAGCGAcg > 1:721535/1‑69 (MQ=255) atgGCGCGCTCCTGTTCCTCTTCATTACTGACTTCGGTCAGCACCCCCATCTCCAGACTGTGAGCGAcg > 1:3180809/1‑69 (MQ=255) atgGCGCGCTCCTGTTCCTCTTCATTACTGACTTCGGTCAGCACCCCCATCTCCAGACTGTGAGCGAcg > 1:731232/1‑69 (MQ=255) atgGCGCGCTCCTGTTCCTCTTCATTACTGACTTCGGTCAGCACCCCCATCTCCAGACTGTGAGCGAcg > 1:3174731/1‑69 (MQ=255) atgGCGCGCTCCTGTTCCTCTTCATTACTGACTTCGGTCAGCACCCCCATCTCCAGACTGTGAGCGAcg > 1:1680858/1‑69 (MQ=255) atgGCGCGCTCCTGTTCCTCTTCATTACTGACTTCGGTCAGCACCCCCATCTCCAGACTGTGAGCGAcg > 1:1706036/1‑69 (MQ=255) atgGCGCGCTCCTGTTCCTCTTCATTACTGACTTCGGTCAGCACCCCCATCTCCAGACTGTGAGCGAcg > 1:2817930/1‑69 (MQ=255) atgGCGCGCTCCTGTTCCTCTTCATTACTGACTTCGGTCAGCACCCCCATCTCCAGACTGTGAGCGAcg > 1:2560274/1‑69 (MQ=255) atgGCGCGCTCCTGTTCCTCTTCATTACTGACTTCGGTCAGCACCCCCATCTCCAGACTGTGAGCGAc > 1:2561732/1‑68 (MQ=255) atgGCGCGCTCCTGTTCCTCTTCATTACTGACTTCGGTCAGCACCCCCATCTACAGACTGTGAGCGAcg > 1:1524420/1‑69 (MQ=255) atgGCGCGCTCCAGTTCCTCTTCATTACTGACTTCGGTCAg < 1:1270469/41‑1 (MQ=255) | TCGACAAATCACGCAGATCGCGGTTGTTGATGCCAACGACCTTTGCTCCCAATGCAATGGCGCGCTCCTGTTCCTCTTCATTACTGACTTCGGTCAGCACCCCCATCTCCAGACTGTGAGCGACG > minE/858163‑858287 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |