| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 860,344 | A→C | 57.6% | T519T (ACT→ACG) | trpE ← | component I of anthranilate synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 860,344 | 0 | A | C | 57.6% | 34.8 / 60.3 | 66 | T519T (ACT→ACG) | trpE | component I of anthranilate synthase |
| Reads supporting (aligned to +/- strand): ref base A (9/19); new base C (18/20); total (27/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.11e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.15e-01 | |||||||||||
TGATCTGCCAGGTTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGTTAC > minE/860280‑860402 | tGATCTGCCAGGTTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGtct < 1:2829279/69‑1 (MQ=255)tGATCTGCCAGGTTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGtct < 1:3202866/69‑1 (MQ=255)tGATCTGCCAGGTTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGtct < 1:2070492/69‑1 (MQ=255)tGATCTGCCAGGTTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGtct < 1:523122/69‑1 (MQ=255)tGATCTGCCAGGTTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGtct < 1:1955262/69‑1 (MQ=255)tGATCTGCCAGGTTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGtct < 1:1273756/69‑1 (MQ=255) gTTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCatga > 1:3213245/1‑69 (MQ=255) gTTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCatga > 1:272295/1‑69 (MQ=255) gTTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCatga > 1:2024916/1‑69 (MQ=255) tGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATTAGAACGTCTCCTGTGc > 1:1729004/1‑63 (MQ=255) tGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATTAGAACGTCTCCTGTGCa > 1:2498542/1‑64 (MQ=255) tGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATTAGAACGTCTCCTGTGCa > 1:2347816/1‑64 (MQ=255) tGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATTAGAACGTCTCCTGTGCa > 1:2123767/1‑64 (MQ=255) tGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATTAGAACGTCTCCTGTGCa > 1:2777144/1‑64 (MQ=255) tGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATTAGAACGTCTCCTGTGCa > 1:2262597/1‑64 (MQ=255) tGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATTAGAACGTCTCCTGTGCa > 1:1750978/1‑64 (MQ=255) tGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATTAGAACGTCTCCTGTGCa > 1:1577276/1‑64 (MQ=255) tGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATTAGAACGTCTCCTGTGCa > 1:1682718/1‑64 (MQ=255) tGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATTAGAACGTCTCCTGTGCa > 1:161617/1‑64 (MQ=255) tGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATTAGAACGTCTCCTGTGCa > 1:3113416/1‑64 (MQ=255) tGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATTAGAACGTCTCCTGTGCa > 1:1274510/1‑64 (MQ=255) tGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATTAGAACGTCTCCTGTGCa > 1:379127/1‑64 (MQ=255) tGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATTAGAACGTCTCCTGTGCa > 1:1216900/1‑64 (MQ=255) tGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATTAGAACGTCTCCTGTGCa > 1:712608/1‑64 (MQ=255) tGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATTAGAACGTCTCCTGTGCa > 1:760115/1‑64 (MQ=255) tGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATTAGAACGTCTCCTGAGCa > 1:498992/1‑64 (MQ=255) tGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATTAGAACGTCCCCTGTGCa > 1:115433/1‑64 (MQ=255) gTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCatgatg > 1:760473/1‑68 (MQ=255) gTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATgc > 1:808009/1‑69 (MQ=255) cGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATGcgcg > 1:1058352/1‑69 (MQ=255) cGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATGcgcg > 1:597961/1‑69 (MQ=255) cGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATGcgcg > 1:1448499/1‑69 (MQ=255) gTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATgcgc < 1:797916/67‑1 (MQ=255) agagTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGc < 1:3211059/68‑1 (MQ=255) agagTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGc < 1:1138000/68‑1 (MQ=255) agagTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGc < 1:1505209/68‑1 (MQ=255) gagTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCaa < 1:1117111/69‑1 (MQ=255) tCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGtct < 1:1510488/42‑1 (MQ=255) gAATGTCAGCCATTAGAACGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCTGTACAGCGCGGGc < 1:1261639/69‑1 (MQ=255) gAATGTCAGCCATTAGAACGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGc < 1:2079218/69‑1 (MQ=255) gAATGTCAGCCATTAGAACGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGc < 1:2708357/69‑1 (MQ=255) gAATGTCAGCCATTAGAACGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGc < 1:23537/69‑1 (MQ=255) gAATGTCAGCCATTAGAACGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGc < 1:78283/69‑1 (MQ=255) gAATGTCAGCCATTAGAACGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGc < 1:1095178/69‑1 (MQ=255) gAATGTCAGCCATTAGAACGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGc < 1:2447646/69‑1 (MQ=255) gAATGTCAGCCATTAGAACGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGc < 1:688358/69‑1 (MQ=255) gAATGTCAGCCATTAGAACGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGc < 1:2027311/69‑1 (MQ=255) gAATGTCAGCCATTAGAACGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGc < 1:534621/69‑1 (MQ=255) gAATGTCAGCCATTAGAACGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGc < 1:118840/69‑1 (MQ=255) gAATGTCAGCCATTAGAACGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGc < 1:470647/69‑1 (MQ=255) gAATGTCAGCCATTAGAACGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGc < 1:328834/69‑1 (MQ=255) gAATGTCAGCCATTAGAACGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGc < 1:2625131/69‑1 (MQ=255) gAATGTCAGCCATTAGAACGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGc < 1:2251513/69‑1 (MQ=255) gAATGTCAGCCATTAGAACGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGc < 1:3003568/69‑1 (MQ=255) gAATGTCAGCCATTAGAACGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGc < 1:1915957/69‑1 (MQ=255) gAATGTCAGCCATTAGAACGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGc < 1:2769890/69‑1 (MQ=255) aaTGTCAGCCATTAGAACGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGc < 1:1201076/68‑1 (MQ=255) aaTGTCAGCCATTAGAACGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGc < 1:3061788/68‑1 (MQ=255) tCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGt < 1:354856/69‑1 (MQ=255) tCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGt < 1:2665921/69‑1 (MQ=255) tCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGt < 1:571651/69‑1 (MQ=255) tCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGt < 1:590950/69‑1 (MQ=255) tCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGt < 1:3162311/69‑1 (MQ=255) tCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGt < 1:2902089/69‑1 (MQ=255) tCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGt < 1:1935637/69‑1 (MQ=255) gCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGTTAc > 1:1885557/1‑69 (MQ=255) | TGATCTGCCAGGTTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGTTAC > minE/860280‑860402 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |