| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 860,884 | T→C | 48.3% | S339S (TCA→TCG) | trpE ← | component I of anthranilate synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 860,884 | 0 | T | C | 48.3% | ‑2.0 / 49.8 | 58 | S339S (TCA→TCG) | trpE | component I of anthranilate synthase |
| Reads supporting (aligned to +/- strand): ref base T (11/19); new base C (12/16); total (23/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.89e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.47e-01 | |||||||||||
GTTCAGACAGCTCTTTATGATCGGTACGCATTTCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGCGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCA > minE/860817‑860938 | gTTCAGACAGCTCTTTATGATCGGTACGCATTTCCAGTTCAATACGTCTGTCGAGATCTCTGTCCAGTg < 1:391299/69‑1 (MQ=255)gTTCAGACAGCTCTTTATGATCGGTACGCATTTCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGtc < 1:2684958/69‑2 (MQ=255)gTTCAGACAGCTCTTTATGATCGGTACGCATTTCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTg < 1:1016324/69‑1 (MQ=255)gTTCAGACAGCTCTTTATGATCGGTACGCATTTCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTg < 1:1084000/69‑1 (MQ=255)gTTCAGACAGCTCTTTATGATCGGTACGCATTTCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTg < 1:602150/69‑1 (MQ=255)gTTCAGACAGCTCTTTATGATCGGTACGCATTTCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTg < 1:2771760/69‑1 (MQ=255)gTTCAGACAGCTCTTTATGATCGGTACGCATTTCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTg < 1:2530049/69‑1 (MQ=255)gTTCAGACAGCTCTTTATGATCGGTACACATTTCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTg < 1:3124248/69‑1 (MQ=255) ttCAGACAGCTCTTTATGATCGGTACGCATTTCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTg < 1:2660456/68‑1 (MQ=255) ttCAGACAGCTCTTTATGATCGGTACGCATTTCCAGTTAAATACGGCTGTCGAGATCTCTGTCCAGTg < 1:1568619/68‑1 (MQ=255) tCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCg > 1:891967/1‑45 (MQ=255) tCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGCGCGCCGACCGCGTGGGCGTGtt > 1:1950235/1‑69 (MQ=255) tCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGCGCGACGACCGCGTGGGCGTGtt > 1:2477674/1‑69 (MQ=255) tCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGCGCGACGACCGCGTGGGCGTGtt > 1:2212732/1‑69 (MQ=255) cAATACGGCTGTCGAGGTCTCTGTCCAGTGAACCATCGGCGCGACGACCGCGTGGGCGTGTTCCGGCaa < 1:984551/69‑1 (MQ=255) cAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGCGCGACGACCGCGTGGGCGTGTTCCGGCaa < 1:526465/69‑1 (MQ=255) cAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGCGCGACGACCGCGTGGGCGTGTTCCGGCaa < 1:2775642/69‑1 (MQ=255) cAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGCGCGACGACCGCGTGGGCGTGTTCCGGCaa < 1:2948501/69‑1 (MQ=255) cAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGCGCGACGACCGCGTGGGCGTGTTCCGGCaa < 1:2546400/69‑1 (MQ=255) cAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGCGCGACGACCGCGTGGGCGTGTTCCGGCaa < 1:896199/69‑1 (MQ=255) cAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGCGCGACGACCGCGTGGGCGTGTTCCGGCaa < 1:1041054/69‑1 (MQ=255) aCGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGCGCGACGACCGCGTGGGCGTGTTCCGGCAATCg > 1:2189140/1‑68 (MQ=255) aCGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGCGCGACGACCGCGTGGGCGTGTTCCGGCAATCg > 1:2196463/1‑68 (MQ=255) aCGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGCGCGACGACCGCGTGGGCGTGTTCCGGCAATCg > 1:2358693/1‑68 (MQ=255) gCTGTCGAGGTCTCTGTCCAGCGAACCATCGGCACGACGACCGCGTGGACGTGTTCCGGCAATCGGGTa < 1:643421/69‑1 (MQ=255) gCTGTCGAGGTCTCTGTCCAGCGAACCATCGGCACGACGACCGCGTGGACGTGTTCCGGCAATCGGGTa < 1:549794/69‑1 (MQ=255) gCTGTCGAGGTCTCTGTCCAGCGAACCATCGGCACGACGACCGCGTGGACGTGTTCCGGCAATCGGGTa < 1:2882868/69‑1 (MQ=255) gCTGTCGAGGTCTCTGTCCAGCGAACCATCGGCACGACGACCGCGTGGACGTGTTCCGGCAATCGGGTa < 1:494994/69‑1 (MQ=255) gCTGTCGAGGTCTCTGTCCAGCGAACCATCGGCACGACGACCGCGTGGACGTGTTCCGGCAATCGGGTa < 1:1414630/69‑1 (MQ=255) gCTGTCGAGGTCTCTGTCCAGCGAACCATCGGCACGACGACCGCGTGGACGTGTTCCGGCAATCGGGTa < 1:3180724/69‑1 (MQ=255) gCTGTCGAGGTCTCTGTCCAGCGAACCATCGGCACGACGACCGCGTGGACGTGTTCCGGCAATCGGGTa < 1:811797/69‑1 (MQ=255) gCTGTCGAGGTCTCTGTCCAGCGAACCATCGGCACGACGACCGCGTGGACGTGTTCCGGCAATCGGGTa < 1:2801693/69‑1 (MQ=255) gCTGTCGAGGTCTCTGTCCAGCGAACCATCGGCACGACGACCGCGTGGACGTGTTCCGGCAATCGGGTa < 1:1439586/69‑1 (MQ=255) gCTGTCGAGGTCTCTGTCCAGCGAACCATCGGCACGACGACCGCGTGGACGTGTTCCGGCAATCGGGTa < 1:2165148/69‑1 (MQ=255) gCTGTCGAGGTCTCTGTCCAGCGAACCATCGGCACGACGACCGCGTGGACGTGTTCCGGCAATCGGGTa < 1:1837426/69‑1 (MQ=255) gCTGTCGAGGTCTCTGTCCAGCGAACCATCGGCACGACGACCGCGTGGACGTGTTCCGGCAATCGGGTa < 1:1793868/69‑1 (MQ=255) gCTGTCGAGGTCTCTGTCCAGCGAACCATCGGCACGACGACCGCGTGGACGTGTTCCGGCAATCGGGTa < 1:1812409/69‑1 (MQ=255) gCTGTCGAGGTCTCTGTCCAGCGAACCATCGGCACGACGACCGCGTGGACGTGTTCCGGCAAGCGGGTa < 1:441548/69‑1 (MQ=255) gCTGGCGAGGTCTCTGTCCAGCGAACCATCGGCACGACGACCGCGTGGACGTGTTCCGGCAATCGGGTa < 1:2471902/69‑1 (MQ=255) gaggTCTCTGTCCAGCGAACCATCGGCACGACGACCGCGTGGACGTGTTCCGGCAATCGGGTAAAtctc > 1:1859136/1‑69 (MQ=255) gaggTCTCTGTCCAGCGAACCATCGGCACGACGACCGCGTGGACGTGTTCCGGCAATCGGGTAAAtctc > 1:2138887/1‑69 (MQ=255) gaggTCTCTGTCCAGCGAACCATCGGCACGACGACCGCGTGGACGTGTTCCGGCAATCGGGTAAAtctc > 1:1115292/1‑69 (MQ=255) gaggTCTCTGTCCAGCGAACCATCGGCACGACGACCGCGTGGACGTGTTCCGGCAATCGGGTAAAtctc > 1:2167220/1‑69 (MQ=255) gaggTCTCTGTCCAGCGAACCATCGGCACGACGACCGCGTGGACGTGTTCCGGCAATCGGGTAAAtctc > 1:115005/1‑69 (MQ=255) gaggTCTCTGTCCAGCGAACCATCGGCACGACGACCGCGTGGACGTGTTCCGGCAATCGGGTAAAtctc > 1:2178465/1‑69 (MQ=255) gaggTCTCTGTCCAGCGAACCATCGGCACGACGACCGCGTGGACGTGTTCCGGCAATCGGGTAAAtctc > 1:1435493/1‑69 (MQ=255) gaggTCTCTGTCCAGCGAACCATCGGCACGACGACCGCGTGGACGTGTTCCGGCAATCGGGTAAAtctc > 1:1750680/1‑69 (MQ=255) gaggTCTCTGTCCAGCGAACCATCGGCACGACGACCGCGTGGACGTGTTCCGGCAATCGGGTAAAtctc > 1:1863052/1‑69 (MQ=255) gaggTCTCTGTCCAGCGAACCATCGGCACGACGACCGCGTGGACGTGTTCCGGCAATCGGGTAAAtctc > 1:153102/1‑69 (MQ=255) gaggTCTCTGTCCAGCGAACCATCGGCACGACGACCGCGTGGACGTGTTCCGGCAATCGGGTAAAtctc > 1:2699539/1‑69 (MQ=255) gaggTCTCTGTCCAGCGAACCATCGGCACGACGACCGCGTGGACGTGTTCCGGCAATCGGGTAAAtct > 1:3126606/1‑68 (MQ=255) gagaTCTCTGTCCAGTGAACCATCGGCGCGACGACCGCGTGGGCGTGTTCCGGCAATCgg > 1:283142/1‑60 (MQ=255) gagaTCTCTGTCCAGTGAACCATCGGCGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGAtctc > 1:318677/1‑69 (MQ=255) gagaTCTCTGTCCAGTGAACCATCGGCGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGAtctc > 1:954054/1‑69 (MQ=255) gagaTCTCTGTCCAGTGAACCATCGGCGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGAtctc > 1:965557/1‑69 (MQ=255) gaTCTCTGTCCAGTGAACCATCGGCGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCa < 1:2860483/68‑1 (MQ=255) gaTCTCTGTCCAGTGAACCATCGGCGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCa < 1:816996/68‑1 (MQ=255) gaTCTCTGTCCAGTGAACCATCGGCGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCa < 1:935797/68‑1 (MQ=255) ccAGCGAACCATCGGCACGAAGACCGCGTGGACGTGTTCCGGCAATCGGGTa < 1:743003/52‑1 (MQ=255) | GTTCAGACAGCTCTTTATGATCGGTACGCATTTCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGCGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCA > minE/860817‑860938 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |