| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 860,896 | G→A | 38.3% | R335R (CGC→CGT) | trpE ← | component I of anthranilate synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 860,896 | 0 | G | A | 38.3% | 39.2 / 45.7 | 73 | R335R (CGC→CGT) | trpE | component I of anthranilate synthase |
| Reads supporting (aligned to +/- strand): ref base G (15/30); new base A (12/16); total (27/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.61e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
TCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGCGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCTGGTGGCATCATA > minE/860849‑860961 | tCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGCGCGCCGACCGCGTGGGCGTGtt > 1:1950235/1‑69 (MQ=255)tCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGCGCGACGACCGCGTGGGCGTGtt > 1:2212732/1‑69 (MQ=255)tCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGCGCGACGACCGCGTGGGCGTGtt > 1:2477674/1‑69 (MQ=255) cAATACGGCTGTCGAGGTCTCTGTCCAGTGAACCATCGGCGCGACGACCGCGTGGGCGTGTTCCGGCaa < 1:984551/69‑1 (MQ=255) cAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGCGCGACGACCGCGTGGGCGTGTTCCGGCaa < 1:1041054/69‑1 (MQ=255) cAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGCGCGACGACCGCGTGGGCGTGTTCCGGCaa < 1:896199/69‑1 (MQ=255) cAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGCGCGACGACCGCGTGGGCGTGTTCCGGCaa < 1:526465/69‑1 (MQ=255) cAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGCGCGACGACCGCGTGGGCGTGTTCCGGCaa < 1:2546400/69‑1 (MQ=255) cAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGCGCGACGACCGCGTGGGCGTGTTCCGGCaa < 1:2775642/69‑1 (MQ=255) cAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGCGCGACGACCGCGTGGGCGTGTTCCGGCaa < 1:2948501/69‑1 (MQ=255) aCGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGCGCGACGACCGCGTGGGCGTGTTCCGGCAATCg > 1:2189140/1‑68 (MQ=255) aCGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGCGCGACGACCGCGTGGGCGTGTTCCGGCAATCg > 1:2196463/1‑68 (MQ=255) aCGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGCGCGACGACCGCGTGGGCGTGTTCCGGCAATCg > 1:2358693/1‑68 (MQ=255) gCTGTCGAGGTCTCTGTCCAGCGAACCATCGGCACGACGACCGCGTGGACGTGTTCCGGCAATCGGGTa < 1:2882868/69‑1 (MQ=255) gCTGTCGAGGTCTCTGTCCAGCGAACCATCGGCACGACGACCGCGTGGACGTGTTCCGGCAATCGGGTa < 1:811797/69‑1 (MQ=255) gCTGTCGAGGTCTCTGTCCAGCGAACCATCGGCACGACGACCGCGTGGACGTGTTCCGGCAATCGGGTa < 1:2165148/69‑1 (MQ=255) gCTGTCGAGGTCTCTGTCCAGCGAACCATCGGCACGACGACCGCGTGGACGTGTTCCGGCAATCGGGTa < 1:643421/69‑1 (MQ=255) gCTGTCGAGGTCTCTGTCCAGCGAACCATCGGCACGACGACCGCGTGGACGTGTTCCGGCAATCGGGTa < 1:549794/69‑1 (MQ=255) gCTGTCGAGGTCTCTGTCCAGCGAACCATCGGCACGACGACCGCGTGGACGTGTTCCGGCAATCGGGTa < 1:1414630/69‑1 (MQ=255) gCTGTCGAGGTCTCTGTCCAGCGAACCATCGGCACGACGACCGCGTGGACGTGTTCCGGCAATCGGGTa < 1:494994/69‑1 (MQ=255) gCTGTCGAGGTCTCTGTCCAGCGAACCATCGGCACGACGACCGCGTGGACGTGTTCCGGCAATCGGGTa < 1:1439586/69‑1 (MQ=255) gCTGTCGAGGTCTCTGTCCAGCGAACCATCGGCACGACGACCGCGTGGACGTGTTCCGGCAATCGGGTa < 1:2801693/69‑1 (MQ=255) gCTGTCGAGGTCTCTGTCCAGCGAACCATCGGCACGACGACCGCGTGGACGTGTTCCGGCAATCGGGTa < 1:1837426/69‑1 (MQ=255) gCTGTCGAGGTCTCTGTCCAGCGAACCATCGGCACGACGACCGCGTGGACGTGTTCCGGCAATCGGGTa < 1:1812409/69‑1 (MQ=255) gCTGTCGAGGTCTCTGTCCAGCGAACCATCGGCACGACGACCGCGTGGACGTGTTCCGGCAATCGGGTa < 1:1793868/69‑1 (MQ=255) gCTGTCGAGGTCTCTGTCCAGCGAACCATCGGCACGACGACCGCGTGGACGTGTTCCGGCAATCGGGTa < 1:3180724/69‑1 (MQ=255) gCTGTCGAGGTCTCTGTCCAGCGAACCATCGGCACGACGACCGCGTGGACGTGTTCCGGCAAGCGGGTa < 1:441548/69‑1 (MQ=255) gCTGGCGAGGTCTCTGTCCAGCGAACCATCGGCACGACGACCGCGTGGACGTGTTCCGGCAATCGGGTa < 1:2471902/69‑1 (MQ=255) gaggTCTCTGTCCAGCGAACCATCGGCACGACGACCGCGTGGACGTGTTCCGGCAATCGGGTAAAtctc > 1:2699539/1‑69 (MQ=255) gaggTCTCTGTCCAGCGAACCATCGGCACGACGACCGCGTGGACGTGTTCCGGCAATCGGGTAAAtctc > 1:1115292/1‑69 (MQ=255) gaggTCTCTGTCCAGCGAACCATCGGCACGACGACCGCGTGGACGTGTTCCGGCAATCGGGTAAAtctc > 1:2167220/1‑69 (MQ=255) gaggTCTCTGTCCAGCGAACCATCGGCACGACGACCGCGTGGACGTGTTCCGGCAATCGGGTAAAtctc > 1:115005/1‑69 (MQ=255) gaggTCTCTGTCCAGCGAACCATCGGCACGACGACCGCGTGGACGTGTTCCGGCAATCGGGTAAAtctc > 1:1435493/1‑69 (MQ=255) gaggTCTCTGTCCAGCGAACCATCGGCACGACGACCGCGTGGACGTGTTCCGGCAATCGGGTAAAtctc > 1:153102/1‑69 (MQ=255) gaggTCTCTGTCCAGCGAACCATCGGCACGACGACCGCGTGGACGTGTTCCGGCAATCGGGTAAAtctc > 1:1750680/1‑69 (MQ=255) gaggTCTCTGTCCAGCGAACCATCGGCACGACGACCGCGTGGACGTGTTCCGGCAATCGGGTAAAtctc > 1:1859136/1‑69 (MQ=255) gaggTCTCTGTCCAGCGAACCATCGGCACGACGACCGCGTGGACGTGTTCCGGCAATCGGGTAAAtctc > 1:1863052/1‑69 (MQ=255) gaggTCTCTGTCCAGCGAACCATCGGCACGACGACCGCGTGGACGTGTTCCGGCAATCGGGTAAAtctc > 1:2178465/1‑69 (MQ=255) gaggTCTCTGTCCAGCGAACCATCGGCACGACGACCGCGTGGACGTGTTCCGGCAATCGGGTAAAtctc > 1:2138887/1‑69 (MQ=255) gaggTCTCTGTCCAGCGAACCATCGGCACGACGACCGCGTGGACGTGTTCCGGCAATCGGGTAAAtct > 1:3126606/1‑68 (MQ=255) gagaTCTCTGTCCAGTGAACCATCGGCGCGACGACCGCGTGGGCGTGTTCCGGCAATCgg > 1:283142/1‑60 (MQ=255) gagaTCTCTGTCCAGTGAACCATCGGCGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGAtctc > 1:965557/1‑69 (MQ=255) gagaTCTCTGTCCAGTGAACCATCGGCGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGAtctc > 1:954054/1‑69 (MQ=255) gagaTCTCTGTCCAGTGAACCATCGGCGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGAtctc > 1:318677/1‑69 (MQ=255) gaTCTCTGTCCAGTGAACCATCGGCGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCa < 1:2860483/68‑1 (MQ=255) gaTCTCTGTCCAGTGAACCATCGGCGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCa < 1:816996/68‑1 (MQ=255) gaTCTCTGTCCAGTGAACCATCGGCGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCa < 1:935797/68‑1 (MQ=255) ccAGCGAACCATCGGCACGAAGACCGCGTGGACGTGTTCCGGCAATCGGGTa < 1:743003/52‑1 (MQ=255) cATCGGCGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCTGGTGGcat < 1:3360696/69‑1 (MQ=255) cATCGGCGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCTGGTGGcat < 1:129112/69‑1 (MQ=255) cATCGGCGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCTGGTGGcat < 1:572135/69‑1 (MQ=255) cATCGGCGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCTGGTGGcat < 1:1357536/69‑1 (MQ=255) cATCGGCGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCTGGTGGcat < 1:77310/69‑1 (MQ=255) cATCGGCGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCTGGTGGcat < 1:2052773/69‑1 (MQ=255) cATCGGCGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCTGGTGGcat < 1:3393977/69‑1 (MQ=255) cATCGGCGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCTGGTGGcat < 1:2014867/69‑1 (MQ=255) cATCGGCGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCTGGTGGcat < 1:1966513/69‑1 (MQ=255) cATCGGCGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCTGGTGGcat < 1:2581901/69‑1 (MQ=255) cATCGGCGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCTGGTGGcat < 1:1970897/69‑1 (MQ=255) cATCGGCGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCTGGTGGcat < 1:2789706/69‑1 (MQ=255) cATCGGCGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCTGGTGGcat < 1:2504660/69‑1 (MQ=255) cATCGGCGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCTGGTGGcat < 1:2434804/69‑1 (MQ=255) cATCGGCGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCTGGTGGcat < 1:1668081/69‑1 (MQ=255) cATCGGCGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCTGGTGGcat < 1:2991669/69‑1 (MQ=255) cATCGGCGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCTGGTGGcat < 1:3248748/69‑1 (MQ=255) cATCGGCGCGACGACCGCGTGGGCGTGTTCCGCCAATCGGGTAGATCTCAATCTGGCGGCTGGTGGcat < 1:2700703/69‑1 (MQ=255) tCGGCGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCTGGTGGcatca < 1:128841/69‑1 (MQ=255) tCGGCGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCTGGTGGcatca < 1:1193683/69‑1 (MQ=255) ggCGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCTGGTGGCATCATa > 1:135535/1‑69 (MQ=255) ggCGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCTGGTGGCATCATa > 1:1586457/1‑69 (MQ=255) ggCGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCTGGTGGCATCATa > 1:1946589/1‑69 (MQ=255) ggCGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCTGGTGGCATCATa > 1:3410176/1‑69 (MQ=255) ggCGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCTGGTGGCATCATa > 1:981437/1‑69 (MQ=255) gcgACGACCGCGTGGGCGTGCTCCGGCAATCGGGTAGATCTCAATCTGGCGGCTGGTGGcat < 1:3341159/62‑1 (MQ=255) | TCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGCGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCTGGTGGCATCATA > minE/860849‑860961 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |