Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 863,516 G→C 59.2% S155S (TCG→TCC yciO → conserved hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE863,5160GC59.2% 21.1 / 46.4 49S155S (TCG→TCCyciOconserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (15/5);  new base C (7/22);  total (22/27)
Fisher's exact test for biased strand distribution p-value = 1.04e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.94e-01

TGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCAC  >  minE/863450‑863570
                                                                  |                                                      
tGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGa                                                      <  1:1712829/69‑1 (MQ=255)
         gggCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGTGAATTTACCGAATCCGATCCGgaaga                                             >  1:2289408/1‑69 (MQ=255)
         gggCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGTGAATTTACCGAATCCGATCCGgaaga                                             >  1:1858419/1‑69 (MQ=255)
         gggCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGTGAATTTACCGAATCCGATCCGgaaga                                             >  1:2642613/1‑69 (MQ=255)
         gggCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGTGAATTTACCGAATCCGATCCGgaaga                                             >  1:2792729/1‑69 (MQ=255)
         gggCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGTGAATTTACCGAATCCGATCCGgaaga                                             >  1:1451939/1‑69 (MQ=255)
         gggCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGTGAATTTACCGAATCCGATCCGgaaga                                             >  1:2975685/1‑69 (MQ=255)
         gggCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGTGAATTTACCGAATCCGATCCGgaag                                              >  1:1522780/1‑68 (MQ=255)
            cGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAAt                                          <  1:2108472/69‑1 (MQ=255)
            cGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAAt                                          <  1:1836805/69‑1 (MQ=255)
            cGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAAt                                          <  1:1437190/69‑1 (MQ=255)
               aCCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGTGAATTTACCGTATCCGa                                                      <  1:435361/54‑1 (MQ=38)
               aCCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGTGAATTTACCGAATCCGa                                                      <  1:3285009/54‑1 (MQ=39)
               aCCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGTGAATTTACCGAATCCGa                                                      <  1:466335/54‑1 (MQ=39)
               aCCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGTGAATTTACCGAATCCGa                                                      <  1:2865530/54‑1 (MQ=39)
               aCCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGTGAATTTACCGAATCCGa                                                      <  1:2743101/54‑1 (MQ=39)
               aCCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGTGAATTTACCGAATCCGa                                                      <  1:549036/54‑1 (MQ=39)
               aCCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGTGAATTTACCGAATCCGa                                                      <  1:2526451/54‑1 (MQ=39)
               aCCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGTGAATTTACCGAATCCGa                                                      <  1:2377969/54‑1 (MQ=39)
               aCCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGTGAATTTACCGAATCCGa                                                      <  1:742072/54‑1 (MQ=39)
               aCCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGTGAATTTACCGAATCCGa                                                      <  1:761902/54‑1 (MQ=39)
               aCCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGTGAATTTACCGAATCCGa                                                      <  1:116368/54‑1 (MQ=39)
               aCCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGTGAATTTACCGAATCCGa                                                      <  1:787887/54‑1 (MQ=39)
               aCCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGTGAATTTACCGAATCCGa                                                      <  1:1519599/54‑1 (MQ=39)
               aCCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGTGAATTTACCGAATCCGa                                                      <  1:1164029/54‑1 (MQ=39)
               aCCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGTGAATTTACCGAATCCGa                                                      <  1:120289/54‑1 (MQ=39)
               aCCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGTGAATTTACCGAATCCGa                                                      <  1:1256091/54‑1 (MQ=39)
               aCCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGTGAATTTACCGAATCCGa                                                      <  1:1389228/54‑1 (MQ=39)
               aCCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGTGAATTTACCGAATCCGa                                                      <  1:1496978/54‑1 (MQ=39)
               aCCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGTGAATTTACCGAATCCGa                                                      <  1:175715/54‑1 (MQ=39)
               aCCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGTGAATTTACCGAATCCGa                                                      <  1:1760269/54‑1 (MQ=39)
               aCCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGTGAATTTACCGAATCCGa                                                      <  1:1895606/54‑1 (MQ=39)
               aCCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGTGAATTTACCGAATCCGa                                                      <  1:1768986/54‑1 (MQ=39)
                 cGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAg                                     <  1:357499/69‑1 (MQ=255)
                               tCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGaaa                         >  1:1769075/1‑67 (MQ=255)
                               tCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGaaa                         >  1:1826067/1‑67 (MQ=255)
                               tCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGaaa                         >  1:1784119/1‑67 (MQ=255)
                               tCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGaaa                         >  1:509249/1‑67 (MQ=255)
                               tCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGaaa                         >  1:1508529/1‑67 (MQ=255)
                               tCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGaaa                         >  1:1963385/1‑67 (MQ=255)
                               tCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGaaa                         >  1:3191097/1‑67 (MQ=255)
                               tCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGaaa                         >  1:3095644/1‑67 (MQ=255)
                                        ctgcCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGa               >  1:1766796/1‑68 (MQ=255)
                                        ctgcCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGAt              >  1:622808/1‑69 (MQ=255)
                                        ctgcCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGAt              >  1:297511/1‑69 (MQ=255)
                                               gCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGatta       >  1:574778/1‑69 (MQ=255)
                                               gCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGatt        >  1:684366/1‑68 (MQ=255)
                                                    gAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCAc  >  1:447184/1‑69 (MQ=255)
                                                    gAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCAc  >  1:254742/1‑69 (MQ=255)
                                                                  |                                                      
TGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCAC  >  minE/863450‑863570

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: