| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 866,934 | A→G | 62.7% | L126L (CTT→CTC) | btuR ← | cob(I)alamin adenolsyltransferase/cobinamide ATP‑dependent adenolsyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 866,934 | 0 | A | G | 62.7% | 55.7 / 55.5 | 91 | L126L (CTT→CTC) | btuR | cob(I)alamin adenolsyltransferase/cobinamide ATP‑dependent adenolsyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (16/18); new base G (50/7); total (66/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.76e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.32e-01 | |||||||||||
TAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGTTGCCAGACTT > minE/866871‑866992 | tAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCaa > 1:2282401/1‑69 (MQ=255)tAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCaa > 1:17593/1‑69 (MQ=255)tAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCaa > 1:916833/1‑69 (MQ=255) gCCTGCACCACTTCTTCCAGTGTCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAAc < 1:2303478/68‑1 (MQ=255) gCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAAc < 1:311990/68‑1 (MQ=255) gCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAAc < 1:306122/68‑1 (MQ=255) gCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAAc < 1:2414063/68‑1 (MQ=255) gCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAAc < 1:2284739/68‑1 (MQ=255) gCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAAc < 1:947811/68‑1 (MQ=255) gCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAAc < 1:1929552/68‑1 (MQ=255) gCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAAc < 1:784892/68‑1 (MQ=255) gCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAAc < 1:154131/68‑1 (MQ=255) gCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAAc < 1:2044320/68‑1 (MQ=255) ccacTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATcc < 1:489697/68‑1 (MQ=255) ccacTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATcc < 1:1962987/68‑1 (MQ=255) ccacTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATcc < 1:2294648/68‑1 (MQ=255) ccacTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATcc < 1:1099887/68‑1 (MQ=255) ccacTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATcc < 1:2841626/68‑1 (MQ=255) ccacTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATcc < 1:3232822/68‑1 (MQ=255) ccacTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATcc < 1:1204884/68‑1 (MQ=255) ccacTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGGCAGTTCATCAAGCAAAACCATATcc < 1:3299430/68‑1 (MQ=255) acTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCGAGCAAAAcc > 1:3430688/1‑60 (MQ=255) acTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCGAGCAAAAcc > 1:3405306/1‑60 (MQ=255) acTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCGAGCAAAAcc > 1:2328917/1‑60 (MQ=255) acTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCGAGCAAAAcc > 1:2699596/1‑60 (MQ=255) acTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCGAGCAAAAcc > 1:110861/1‑60 (MQ=255) acTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCGAGCAAAAcc > 1:329658/1‑60 (MQ=255) acTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCGAGCAAAAcc > 1:2791907/1‑60 (MQ=255) acTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCGAGCAAAAcc > 1:3290819/1‑60 (MQ=255) acTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCGAGCAAAAcc > 1:543577/1‑60 (MQ=255) acTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCGAGCAAAAcc > 1:2841248/1‑60 (MQ=255) acTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCGAGCAAAAcc > 1:2928682/1‑60 (MQ=255) acTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCGAGCAAAAcc > 1:3126250/1‑60 (MQ=255) acTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCGAGCAAAAcc > 1:1539237/1‑60 (MQ=255) acTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCGAGCAAAAcc > 1:2072989/1‑60 (MQ=255) acTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCGAGCAAAAcc > 1:850082/1‑60 (MQ=255) acTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCGAGCAAAAcc > 1:1867489/1‑60 (MQ=255) acTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCGAGCAAAAcc > 1:1170071/1‑60 (MQ=255) acTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCGAGCAAAAcc > 1:1863634/1‑60 (MQ=255) acTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCGAGCAAAAcc > 1:964228/1‑60 (MQ=255) acTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCGAGCAAAAcc > 1:1429816/1‑60 (MQ=255) acTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCGAGCAAAAcc > 1:1740115/1‑60 (MQ=255) acTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCGAGCAAAAcc > 1:1473511/1‑60 (MQ=255) acTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCGAGCAAAAc > 1:1317939/1‑59 (MQ=255) acTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCGAGCAAAAc > 1:3234861/1‑59 (MQ=255) acTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCGAGCAAAAc > 1:1747798/1‑59 (MQ=255) acTTCTTCCAGTGGCAAATAGTCATACGCCACCATATAAGTCAGTTCATCGAGCAAAAcc > 1:914360/1‑60 (MQ=255) ccAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCGAGCAAAACCATATCCAGTGAGGa > 1:979762/1‑67 (MQ=255) ccAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCGAGCAAAACCATATCCAGTGAGGa > 1:586077/1‑67 (MQ=255) ccAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCGAGCAAAACCATATCCAGTGAGGa > 1:314932/1‑67 (MQ=255) ccAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCGAGCAAAACCATATCCAGTGAGGa > 1:845414/1‑67 (MQ=255) ccAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCGAGCAAAACCATATCCAGTGAGGa > 1:3377045/1‑67 (MQ=255) ccAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCGAGCAAAACCATATCCAGTGAGGa > 1:842195/1‑67 (MQ=255) ccAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCGAGCAAAACCATATCCAGTGAGGa > 1:81866/1‑67 (MQ=255) ccAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCGAGCAAAACCATATCCAGTGAGGa > 1:813911/1‑67 (MQ=255) ccAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCGAGCAAAACCATATCCAGTGAGGa > 1:536532/1‑67 (MQ=255) ccAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCGAGCAAAACCATATCCAGTGAGGa > 1:793278/1‑67 (MQ=255) ccAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCGAGCAAAACCATATCCAGTGAGGa > 1:7838/1‑67 (MQ=255) ccAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCGAGCAAAACCATATCCAGTGAGGa > 1:115876/1‑67 (MQ=255) ccAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCGAGCAAAACCATATCCAGTGAGGa > 1:2126246/1‑67 (MQ=255) ccAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCGAGCAAAACCATATCCAGTGAGGa > 1:3127620/1‑67 (MQ=255) ccAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCGAGCAAAACCATATCCAGTGAGGa > 1:1990398/1‑67 (MQ=255) ccAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCGAGCAAAACCATATCCAGTGAGGa > 1:2298704/1‑67 (MQ=255) ccAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCGAGCAAAACCATATCCAGTGAGGa > 1:2384398/1‑67 (MQ=255) ccAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCGAGCAAAACCATATCCAGTGAGGa > 1:2419517/1‑67 (MQ=255) ccAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCGAGCAAAACCATATCCAGTGAGGa > 1:1714026/1‑67 (MQ=255) ccAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCGAGCAAAACCATATCCAGTGAGGa > 1:2677294/1‑67 (MQ=255) ccAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCGAGCAAAACCATATCCAGTGAGGa > 1:2773614/1‑67 (MQ=255) ccAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCGAGCAAAACCATATCCAGTGAGGa > 1:2901066/1‑67 (MQ=255) ccAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCGAGCAAAACCATATCCAGTGAGGa > 1:2986317/1‑67 (MQ=255) ccAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCGAGCAAAACCATATCCAGTGAGGAAt > 1:3433280/1‑69 (MQ=255) cGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTg > 1:1771199/1‑68 (MQ=255) ccaTATACGTCAGTTCATCGAGCAAAACCATATCCAGTGAGGAATCAGCCAGCATCCGCTTTGCATGtt < 1:909641/69‑1 (MQ=255) ccaTATACGTCAGTTCATCGAGCAAAACCATATCCAGTGAGGAATCAGCCAGCATCCGCTTTGCATGtt < 1:1212032/69‑1 (MQ=255) ccaTATACGTCAGTTCATCGAGCAAAACCATATCCAGTGAGGAATCAGCCAGCATCCGCTTTGCATGtt < 1:2047815/69‑1 (MQ=255) ccaTATACGTCAGTTCATCGAGCAAAACCATATCCAGTGAGGAATCAGCCAGCATCCGCTTTGCATGtt < 1:660748/69‑1 (MQ=255) ccaTATACGTCAGTTCATCGAGCAAAACCATATCCAGTGAGGAATCAGCCAGCATCCGCTTTGCATGtt < 1:773743/69‑1 (MQ=255) ccaTATACGTCAGTTCATCGAGCAAAACCATATCCAGTGAGGAATCAGCCAGCATCCGCTTTGCATGtt < 1:2793432/69‑1 (MQ=255) caTATACGTCAGTTCATCGAGCAAAACCATATCCAGTGAGGAATCAGCCAGCATCCGCTTTGCATGtt < 1:1803343/68‑1 (MQ=255) taCGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGTTGCCAg > 1:571593/1‑69 (MQ=255) taCGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGTTGCCAg > 1:80809/1‑69 (MQ=255) taCGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGTTGCCAg > 1:2546920/1‑69 (MQ=255) taCGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGTTGCCAg > 1:2606428/1‑69 (MQ=255) taCGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGTTGCCAg > 1:168575/1‑69 (MQ=255) taCGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGTTGCCAg > 1:1530893/1‑69 (MQ=255) taCGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGTTGCCAg > 1:1484145/1‑69 (MQ=255) taCGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGTTGCCAg > 1:3166288/1‑69 (MQ=255) cGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGTTGCCAg > 1:2717195/1‑67 (MQ=255) cGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGTTGCCAGAc > 1:2186743/1‑69 (MQ=255) cGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGTTGCCAGAc > 1:1797161/1‑69 (MQ=255) tcggTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGTTGCAAGACtt > 1:1465846/4‑69 (MQ=255) | TAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGTTGCCAGACTT > minE/866871‑866992 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |