| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 871,951 | T→C | 29.7% | D650D (GAT→GAC) | topA → | DNA topoisomerase I, omega subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 871,951 | 0 | T | C | 29.7% | 41.1 / 19.3 | 47 | D650D (GAT→GAC) | topA | DNA topoisomerase I, omega subunit |
| Reads supporting (aligned to +/- strand): ref base T (14/19); new base C (11/3); total (25/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.93e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGCAAAACCACCATTAACCTGGTGCCGGAAAACGAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCACGGCGATG > minE/871889‑872008 | tGCAAAACCACCATTAACCTGGTGCCGGAAAACGAAGTGCTGAACGTGCTGGAAGGTGAAGACGCt > 1:1767471/1‑66 (MQ=255)tGCAAAACCACCATTAACCTGGTGCCGGAAAACGAAGTGCTGAACGTGCTGGAAGGTGAAGACGCTGaa > 1:1022422/1‑69 (MQ=255)tGCAAAACCACCATTAACCTGGTGCCGGAAAACGAAGTGCTGAACGTGCTGGAAGGTGAAGACGCTGaa > 1:3028022/1‑69 (MQ=255)tGCAAAACCACCATTAACCTGGTGCCGGAAAACGAAGTGCTGAACGTGCTGGAAGGTGAAGACGCTGaa > 1:3113/1‑69 (MQ=255)tGCAAAACCACCATTAACCTGGTGCCGGAAAACGAAGTGCTGAACGTGCTGGAAGGTGAAGACGCTGaa > 1:3275707/1‑69 (MQ=255)tGCAAAACCACCATTAACCTGGTGCCGGAAAACGAAGTGCTGAACGTGCTGGAAGGTGAAGACGCTGaa > 1:174647/1‑69 (MQ=255)tGCAAAACCACCATTAACCTGGTGCCGGAAAACGAAGTGCTGAACGTGCTGGAAGGTGAAGACGCTGaa > 1:1699143/1‑69 (MQ=255)tGCAAAACCACCATTAACCTGGTGCCGGAAAACGAAGTGCTGAACGTGCTGGAAGGTGAAGACGCTGaa > 1:1531695/1‑69 (MQ=255)tGCAAAACCACCATTAACCTGGTGCCGGAAAACGAAGTGCTGAACGTGCTGGAAGGTGAAGACGCTGaa > 1:1492473/1‑69 (MQ=255)tGCAAAACCACCATTAACCTGGTGCCGGAAAACGAAGTGCTGAACGTGCTGGAAGGTGAAGACGCTGaa > 1:404945/1‑69 (MQ=255)tGCAAAACCACCATTAACCTGGTGCCGGAAAACGAAGTGCTGAACGTGCTGGAAGGTGAAGACGCTGaa > 1:900650/1‑69 (MQ=255) aaCCTGGTGCCGGAAAACGAAGTGCTGAACGTGCTGGAAGGTGAAGACGCTGaa < 1:1798144/54‑1 (MQ=38) aaCCTGGTGCCGGAAAACGAAGTGCTGAACGTGCTGGAAGGTGAAGACGCTGaa < 1:3011716/54‑1 (MQ=38) aaCCTGGTGCCGGAAAACGAAGTGCTGAACGTGCTGGAAGGTGAAGACGCTGaa < 1:2288071/54‑1 (MQ=38) gCCGGAAAACGAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAGCCAACGCGCTGCGCGCAAAAcg > 1:169946/1‑69 (MQ=255) aaaCGAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGtt > 1:3308221/1‑68 (MQ=255) aaaCGAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTg > 1:777440/1‑69 (MQ=255) aaaCGAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTg > 1:822966/1‑69 (MQ=255) aaaCGAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTg > 1:938815/1‑69 (MQ=255) aaaCGAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTg > 1:1009836/1‑69 (MQ=255) aaaCGAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTg > 1:972674/1‑69 (MQ=255) aaaCGAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTg > 1:2508191/1‑69 (MQ=255) aaaCGAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTg > 1:1364258/1‑69 (MQ=255) aaaCGAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTg > 1:2238051/1‑69 (MQ=255) aaaCGAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTg > 1:2320771/1‑69 (MQ=255) aaGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAAcgt < 1:87375/59‑1 (MQ=255) aaGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAAcgt < 1:523288/59‑1 (MQ=255) aaGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAAcgt < 1:1395157/59‑1 (MQ=255) aaGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAAcgt < 1:444225/59‑1 (MQ=255) aaGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAAcgt < 1:41472/59‑1 (MQ=255) aaGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAAcgt < 1:3420791/59‑1 (MQ=255) aaGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAAcgt < 1:1477900/59‑1 (MQ=255) aaGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAAcgt < 1:2628805/59‑1 (MQ=255) aaGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAAcgt < 1:118355/59‑1 (MQ=255) gAACGTGCTGGAAGGCGAAGATTCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCgg > 1:1941146/1‑69 (MQ=255) gAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCgg > 1:263300/1‑69 (MQ=255) gAACGTGCTGGAAGGCAAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCgg > 1:228978/1‑69 (MQ=255) tGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAAcgt < 1:1291616/47‑1 (MQ=255) aGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCACGGCGATg < 1:1425294/67‑1 (MQ=255) aGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCACGGCGATg < 1:1284992/67‑1 (MQ=255) aGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCACGGCGATg < 1:678550/67‑1 (MQ=255) aGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCACGGCGATg < 1:42285/67‑1 (MQ=255) aGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCACGGCGATg < 1:1803693/67‑1 (MQ=255) aGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCACGGCGATg < 1:1967599/67‑1 (MQ=255) aGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCACGGCGATg < 1:2748421/67‑1 (MQ=255) aGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCACGGCGATg < 1:2369427/67‑1 (MQ=255) aGGCGAAGATGCTGAAACCAACGCGCTGCACGCAAAACGTCGTTGCCCGAAATGCGGCACGGCGATg < 1:520340/67‑1 (MQ=255) | TGCAAAACCACCATTAACCTGGTGCCGGAAAACGAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCACGGCGATG > minE/871889‑872008 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |