| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 872,874 | A→G | 31.0% | T22T (ACA→ACG) | cysB → | DNA‑binding transcriptional dual regulator, O‑acetyl‑L‑serine‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 872,874 | 0 | A | G | 31.0% | 105.6 / 59.9 | 119 | T22T (ACA→ACG) | cysB | DNA‑binding transcriptional dual regulator, O‑acetyl‑L‑serine‑binding |
| Reads supporting (aligned to +/- strand): ref base A (52/30); new base G (33/4); total (85/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.11e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01 | |||||||||||
Only 100 of 119 total aligned reads displayed.
TACAACAACTTCGCTATATTGTTGAGGTGGTCAATCATAACCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTA > minE/872816‑872940 | tACAACAACTTCGCTATATTGTTGAGGTGGTCAATCATAACCTGAATGTCTCATCAACAGCGGAAGGAc > 1:301923/1‑69 (MQ=255) acaacTTCGCTATATTGTTGAGGTGGTCAATCATAACCTGAATGTCTCATCAACAGCGGAAGGACTTTa < 1:2565599/69‑1 (MQ=255) acTTCGCTATATTGTTGAGGTGGTCAATCATAACCTGAATGTCTCATCAACAGCGGAAGGACTTTacac > 1:2095565/1‑69 (MQ=255) acTTCGCTATATTGTTGAGGTGGTCAATCATAACCTGAATGTCTCATCAACAGCGGAAGGACTTTacac > 1:2902379/1‑69 (MQ=255) acTTCGCTATATTGTTGAGGTGGTCAATCATAACCTGAATGTCTCATCAACAGCGGAAGGACTTTacac > 1:380082/1‑69 (MQ=255) acTTCGCTATATTGTTGAGGTGGTCAATCATAACCTGAATGTCTCATCAACAGCGGAAGGACTTTacac > 1:1412357/1‑69 (MQ=255) acTTCGCTATATTGTTGAGGTGGTCAATCATAACCTGAATGTCTCATCAACAGCGGAAGGACTTTacac > 1:184537/1‑69 (MQ=255) acTTCGCTATATTGTTGAGGTGGTCAATCATAACCTGAATGTCTCATCAACAGCGGAAGGACTTTacac > 1:2313384/1‑69 (MQ=255) acTTCGCTATATAGTAGAGGTGGTCAATCATAACCTGAATGTCTCATCAACAGCGGAAGGACTTTaca > 1:357934/1‑68 (MQ=255) cTATATTGTTGAGGTGGTCAATCATAACCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATcac < 1:2772919/68‑1 (MQ=255) cTATATTGTTGAGGTGGTCAATCATAACCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATcac < 1:842848/68‑1 (MQ=255) cTATATTGTTGAGGTGGTCAATCATAACCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATcac < 1:2214226/68‑1 (MQ=255) ttgttgAGGTGGTCAATCATAACCTGAATGTCTCATCAACAGCgg > 1:1924652/1‑45 (MQ=255) ttgttgAGGTGGTCAATCATAACCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAAccc > 1:216331/1‑68 (MQ=255) ttgttgAGGTGGTCAATCATAACCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAAccc > 1:528706/1‑68 (MQ=255) ttgttgAGGTGGTCAATCATAACCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAAccc > 1:3092661/1‑68 (MQ=255) ttgttgAGGTGGTCAATCATAACCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAAccc > 1:823923/1‑68 (MQ=255) ttgttgAGGTGGTCAATCATAACCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAAccc > 1:1185937/1‑68 (MQ=255) ttgttgAGGTGGTCAATCATAACCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAAccc > 1:3005277/1‑68 (MQ=255) tgttgAGGTGGTCAATCATAACCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCgg > 1:938979/1‑69 (MQ=255) tgttgAGGTGGTCAATCATAACCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCgg > 1:196806/1‑69 (MQ=255) gttgAGGTGGTCAATCATAACCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCggg < 1:2595872/69‑1 (MQ=255) gttgAGGTGGTCAATCATAACCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCggg < 1:490828/69‑1 (MQ=255) gtggtCAATCATAACCTGAATGTCTCATCAACAGCGGAAGGACtt > 1:3323380/1‑45 (MQ=255) gtggtCAATCATAACCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCTGGATc > 1:1878050/1‑66 (MQ=255) gtggtCAATCATAACCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAg > 1:1535341/1‑68 (MQ=255) gtggtCAATCATAACCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAg > 1:1450903/1‑68 (MQ=255) gtggtCAATCATAACCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAg > 1:1416273/1‑68 (MQ=255) gtggtCAATCATAACCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAg > 1:901789/1‑68 (MQ=255) gtCAATCATAACCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTaa > 1:1782501/1‑68 (MQ=255) gtCAATCATAACCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTaa > 1:718751/1‑68 (MQ=255) gcAATCATAACCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCggg < 1:2981212/58‑1 (MQ=255) aaTCATAACCTGAATGTCTCATCAACAGCGGAAGGa > 1:1003158/1‑36 (MQ=255) aaTCATAACCTGAATGTCTCATCAACAGCGGAAGGa > 1:239621/1‑36 (MQ=255) aaTCATAACCTGAATGTCTCATCAACAGCGGAAGGa > 1:2469108/1‑36 (MQ=255) aaTCATAACCTGAATGTCTCATCAACAGCGGAAGGa > 1:630253/1‑36 (MQ=255) aaTCATAACCTGAATGTCTCATCAACAGCGGAAGGa > 1:650035/1‑36 (MQ=255) aaTCATAACCTGAATGTCTCATCAACAGCGGAAGGa > 1:1539852/1‑36 (MQ=255) aTCATAACCTGAATGTCTCATCAACGGCGGAAGGACTTTACACATCACAAc > 1:2708818/1‑51 (MQ=255) aTCATAACCTGAATGTCTCATCAACGGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACaa > 1:3383787/1‑68 (MQ=255) aTCATAACCTGAATGTCTCATCAACGGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACaa > 1:2880913/1‑68 (MQ=255) aTCATAACCTGAATGTCTCATCAACGGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACaa > 1:2867346/1‑68 (MQ=255) aTCATAACCTGAATGTCTCATCAACGGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACaa > 1:2894023/1‑68 (MQ=255) aTCATAACCTGAATGTCTCATCAACGGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACaa > 1:1220775/1‑68 (MQ=255) aTCATAACCTGAATGTCTCATCAACGGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACaa > 1:1488474/1‑68 (MQ=255) aTCATAACCTGAATGTCTCATCAACGGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACaa > 1:1531173/1‑68 (MQ=255) aTCATAACCTGAATGTCTCATCAACGGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACaa > 1:2632408/1‑68 (MQ=255) aTCATAACCTGAATGTCTCATCAACGGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACaa > 1:3088013/1‑68 (MQ=255) aTCATAACCTGAATGTCTCATCAACGGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACaa > 1:2528581/1‑68 (MQ=255) aTCATAACCTGAATGTCTCATCAACGGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACaa > 1:1609141/1‑68 (MQ=255) aTCATAACCTGAATGTCTCATCAACGGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACaa > 1:246656/1‑68 (MQ=255) aTCATAACCTGAATGTCTCATCAACGGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACaa > 1:2456867/1‑68 (MQ=255) aTCATAACCTGAATGTCTCATCAACGGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACaa > 1:692226/1‑68 (MQ=255) aTCATAACCTGAATGTCTCATCAACGGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACaa > 1:1702670/1‑68 (MQ=255) aTCATAACCTGAATGTCTCATCAACGGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACaa > 1:1738874/1‑68 (MQ=255) aTCATAACCTGAATGTCTCATCAACGGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACaa > 1:2204022/1‑68 (MQ=255) aTCATAACCTGAATGTCTCATCAACGGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACaa > 1:3335541/1‑68 (MQ=255) aTCATAACCCTAATGTCTCATCAACGGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACaa > 1:1581657/1‑68 (MQ=255) aTAACCTGAATGTCTCATGAACGGCGGAAGGACTTTa > 1:1419579/1‑37 (MQ=37) aTAACCTGAATGTCTCATCAACGGCGGAAGGACTTTa > 1:3018169/1‑37 (MQ=255) aTAACCTGAATGTCTCATCAACGGCGGAAGGACTTTa > 1:106375/1‑37 (MQ=255) aTAACCTGAATGTCTCATCAACGGCGGAAGGACTTTa > 1:326735/1‑37 (MQ=255) aTAACCTGAATGTCTCATCAACGGCGGAAGGACTTTa > 1:1610127/1‑37 (MQ=255) aTAACCTGAATGTCTCATCAACGGCGGAAGGACTTTa > 1:1873853/1‑37 (MQ=255) aTAACCTGAATGTCTCATCAACGGCGGAAGGACTTTa > 1:2720464/1‑37 (MQ=255) aTAACCTGAATGTCTCATCAACGGCGGAAGGACTTTa > 1:2069884/1‑37 (MQ=255) aTAACCTGAATGTCTCATCAACGGCGGAAGGACTTTa > 1:2585188/1‑37 (MQ=255) aTAACCTGAATGTCTCATCAACGGCGGAAGGACTTTa > 1:2097086/1‑37 (MQ=255) aTAACCTGAATGTCTCATCAACAGCGGAAGGACTTTa > 1:867835/1‑37 (MQ=255) aTAACCTGAATGTCTCATCAACAGCGGAAGGACTTTa < 1:3341855/37‑1 (MQ=255) aTAACCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTc > 1:827699/1‑68 (MQ=255) aTAACCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTc > 1:803107/1‑68 (MQ=255) aTAACCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTc > 1:981878/1‑68 (MQ=255) aTAACCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTc > 1:2280229/1‑68 (MQ=255) aTAACCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTc > 1:274757/1‑68 (MQ=255) aTAACCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTc > 1:2740233/1‑68 (MQ=255) aTAACCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTc > 1:3226943/1‑68 (MQ=255) aTAACCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTc > 1:2720987/1‑68 (MQ=255) aaCCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAg < 1:3278794/68‑1 (MQ=255) aaCCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAg < 1:1680410/68‑1 (MQ=255) aaCCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAg < 1:1911561/68‑1 (MQ=255) aaCCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAg < 1:1947356/68‑1 (MQ=255) aaCCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAg < 1:398881/68‑1 (MQ=255) aaCCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAg < 1:2377606/68‑1 (MQ=255) aaCCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAg < 1:1074052/68‑1 (MQ=255) aCCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAg < 1:8936/67‑1 (MQ=255) aCCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAg < 1:2863042/67‑1 (MQ=255) tctcATCAACGGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAg < 1:2989137/69‑1 (MQ=255) tctcATCAACGGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAg < 1:124198/69‑1 (MQ=255) tctcATCAACGGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAg < 1:71434/69‑1 (MQ=255) tctcATCAACGGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAg < 1:2152186/69‑1 (MQ=255) caACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACg < 1:987308/66‑1 (MQ=255) caACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACg < 1:1873515/66‑1 (MQ=255) caACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACg < 1:2116939/66‑1 (MQ=255) caACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACg < 1:3273514/66‑1 (MQ=255) caACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACg < 1:2640888/66‑1 (MQ=255) aaCAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCt > 1:395945/1‑69 (MQ=255) cAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTa < 1:1551379/68‑1 (MQ=255) cAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTa < 1:1358554/68‑1 (MQ=255) cAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTa < 1:2257792/68‑1 (MQ=255) | TACAACAACTTCGCTATATTGTTGAGGTGGTCAATCATAACCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTA > minE/872816‑872940 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |