Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	876,010	A→T	22.1%	Q409L (CAG→CTG)	acnA →	aconitate hydratase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	876,010	0	A	T	22.1%	147.2 / 37.5	86	Q409L (CAG→CTG)	acnA	aconitate hydratase 1
Reads supporting (aligned to +/- strand):  ref base A (20/47);  new base T (3/16);  total (23/63)
Fisher's exact test for biased strand distribution p-value = 2.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.24e-01

CCCGATGTACCAAAAGCATTTGCCGCCAGTAACGAACTGGAAGTGAATGCCACGCATAAAGATCGCCAGCCGGTCGATTATGTTATGAACGGACATCAGTATCAGTTACCTGATGGCGCTGTGGTCATTGC  >  minE/875943‑876073                                                                    |                                                                cccGATGTACCAAAAGCATTTGCCGCCAGTAACGAACTGGAAGTGAATGCCACGCATAAAGATCGCCAg                                                                >  1:3054232/1‑69 (MQ=255) cccGATGTACCAAAAGCATTTGCCGCCAGTAACGAACTGGAAGTGAATGCCACGCATAAAGATCGCCAg                                                                >  1:1087011/1‑69 (MQ=255) cccGATGTACCAAAAGCATTTGCCGCCAGTAACGAACTGGAAGTGAATGCCACGCATAAAGATCGCCAg                                                                >  1:799154/1‑69 (MQ=255) cccGATGTACCAAAAGCATTTGCCGCCAGTAACGAACTGGAAGTGAATGCCACGCATAAAGATCGCCAg                                                                >  1:2971068/1‑69 (MQ=255) cccGATGTACCAAAAGCATTTGCCGCCAGTAACGAACTGGAAGTGAATGCCACGCATAAAGATCGCCAg                                                                >  1:1353630/1‑69 (MQ=255) cccGATGTACCAAAAGCATTTGCCGCCAGTAACGAACTGGAAGTGAATGCCACGCATAAAGATCGCCAg                                                                >  1:167936/1‑69 (MQ=255) cccGATGTACCAAAAGCATTTGCCGCCAGTAACGAACTGGAAGTGAATGCCACGCATAAAGATCGCCAg                                                                >  1:2847854/1‑69 (MQ=255) accGATGTACCAAAAGCATTTGCCGCCAGTAACGAACTGGAAGTGAATGCCACGCATAAAGATCGCCa                                                                 >  1:1114150/2‑68 (MQ=255)          ccAAAAGCATTTGCCGCCAGTAACGAACTGGAAGTGAATGCCACGCATAAAGATCGCCAGCCGGTCGAt                                                       <  1:528006/69‑1 (MQ=255)          ccAAAAGCATTTGCCGCCAGTAACGAACTGGAAGTGAATGCCACGCATAAAGATCGCCAGCCGGTCGAt                                                       <  1:2366926/69‑1 (MQ=255)          ccAAAAGCATTTGCCGCCAGTAACGAACTGGAAGTGAATGCCACGCATAAAGATCGCCAGCCGGTCGAt                                                       <  1:3249151/69‑1 (MQ=255)               aGCATTTGCCGCCAGTAACGAACTGGAAGTGAATGCCACGCATAAAGATCGCCAGCCGGTCGAttatgt                                                  <  1:3199148/69‑1 (MQ=255)                   tttGCTGCCAGTAGCGAACTGGAAGTGAATGCCACGCATAAAGATCGCCTGCCGGTCGAttatgttatg                                              >  1:2351196/1‑69 (MQ=255)                   tttGCTGCCAGTAGCGAACTGGAAGTGAATGCCACGCATAAAGATCGCCTGCCGGTCGAttatgttatg                                              >  1:2997486/1‑69 (MQ=255)                   tttGCTGCCAGTAGAGAACTGGAAGTGAATGCCACGCATAAAGATCGCCTGCCGGTCGAttatgttat                                               >  1:1767336/1‑68 (MQ=255)                     tGCCGCCAGTAACGAACTGGAAGTGAATGCCACGCATAAAGATCGCCAGCCGGTCGAt                                                       <  1:675746/58‑1 (MQ=255)                         gccAGTAACGAACTGGAAGTGAATGCCACGCATAAAGATCGCCAGCCGGTCGATTATGTTATGAACGGa                                        <  1:2466605/69‑1 (MQ=255)                         gccAGTAACGAACTGGAAGTGAATGCCACGCATAAAGATCGCCAGCCGGTCGATTATGTTATGAACGGa                                        <  1:2557311/69‑1 (MQ=255)                         gccAGTAACGAACTGGAAGTGAATGCCACGCATAAAGATCGCCAGCCGGTCGATTATGTTATGAACGGa                                        <  1:2065580/69‑1 (MQ=255)                         gccAGTAACGAACTGGAAGTGAATGCCACGCATAAAGATCGCCAGCCGGTCGATTATGTTATGAACGGa                                        <  1:2059948/69‑1 (MQ=255)                         gccAGTAACGAACTGGAAGTGAATGCCACGCATAAAGATCGCCAGCCGGTCGATTATGTTATGAACGGa                                        <  1:2391097/69‑1 (MQ=255)                         gccAGTAACGAACTGGAAGTGAATGCCACGCATAAAGATCGCCAGCCGGTCGATTATGTTATGAACGGa                                        <  1:1606530/69‑1 (MQ=255)                         gccAGTAACGAACTGGAAGTGAATGCCACGCATAAAGATCGCCAGCCGGTCGATTATGTTATGAACGGa                                        <  1:1650062/69‑1 (MQ=255)                         gccAGTAACGAACTGGAAGTGAATGCCACGCATAAAGATCGCCAGCCGGTCGATTATGTTATGAACGGa                                        <  1:1607026/69‑1 (MQ=255)                            aGTAGCGAACTGGAAGTGAATGCCACGCATAAAGATCGCCTGCCGGTCGATTATGTTATGAACGGAc                                       <  1:3110712/67‑1 (MQ=255)                            aGTAGCGAACTGGAAGTGAATGCCACGCATAAAGATCGCCTGCCGGTCGATTATGTTATGAACGGAc                                       <  1:535533/67‑1 (MQ=255)                            aGTAGCGAACTGGAAGTGAATGCCACGCATAAAGATCGCCTGCCGGTCGATTATGTTATGAACGGAc                                       <  1:1433024/67‑1 (MQ=255)                            aGTAGCGAACTGGAAGTGAATGCCACGCATAAAGATCGCCTGCCGGTCGATTATGTTATGAACGGAc                                       <  1:3430555/67‑1 (MQ=255)                            aGTAGCGAACTGGAAGTGAATGCCACGCATAAAGATCGCCTGCCGGTCGATTATGTTATGAACGGAc                                       <  1:262801/67‑1 (MQ=255)                            aGTAGCGAACTGGAAGTGAATGCCACGCATAAAGATCGCCTGCCGGTCGATTATGTTATGAACGGAc                                       <  1:2632515/67‑1 (MQ=255)                            aGTAGCGAACTGGAAGTGAATGCCACGCATAAAGATCGCCTGCCGGTCGATTATGTTATGAACGGAc                                       <  1:2720807/67‑1 (MQ=255)                            aGTAGCGAACTGGAAGTGAATGCCACGCATAAAGATCGCCTGCCGGTCGATTATGTTATGAACGGAc                                       <  1:2091115/67‑1 (MQ=255)                            aGTAGCGAACTGGAAGTGAATGCCACGCATAAAGATCGCCTGCCGGTCGATTATGTTATGAACGGAc                                       <  1:2850672/67‑1 (MQ=255)                            aGTAGCGAACTGGAAGTGAATGCCACGCATAAAGATCGCCTGCCGGTCGATTATGTTATGAACGGAc                                       <  1:3266315/67‑1 (MQ=255)                            aGTAGCGAACTGGAAGTGAATGCCACGCATAAAGATCGCCTGCCGGTCGATTATGTTATGAACGGAc                                       <  1:2867991/67‑1 (MQ=255)                            aGTAGCGAACTGGAAGTGAATGCCACGCATAAAGATCGCCTGCCGGTCGATTATGTTATGAACGGAc                                       <  1:2936390/67‑1 (MQ=255)                            aGTAGCGAACTGGAAGTGAATGCCACGCATAAAGATCGCCTGCCGGTCGATTATGTTATGAACGGAc                                       <  1:1684701/67‑1 (MQ=255)                            aGTAGCGAACTGGAAGTGAATGCCACGCATAAAGATCGCCTGCCGGTCGATTATGTTATGAACGGAc                                       <  1:3010666/67‑1 (MQ=255)                            aGTAGCGAACTGGAAGTGAATGCCACGCATAAAGATCGCCTGCCGGTCGATTATGTTATGAACGGAc                                       <  1:3079110/67‑1 (MQ=255)                            aGTAGCGAACTGGAAGTGAATGCCACGCATAAAGATCGCCTGCCGGTCGATTATGTTATAAACGGAc                                       <  1:1724625/67‑1 (MQ=255)                            aGTAACGAACTGGAAGTGAATGCCACGCATAAAGATCGCCAGCCGGTCGAt                                                       <  1:895598/51‑1 (MQ=255)                                        gAAGTGAATGCCACGCATAAAGATCGCCAGCCGGTCGATTATGTTATGAACGGACatcag                                  <  1:260082/60‑1 (MQ=255)                                        gAAGTGAATGCCACGCATAAAGATCGCCAGCCGGTCGATTATGTTATGAACGGACatcag                                  <  1:1298866/60‑1 (MQ=255)                                           gTGAATGCCACGCATAAAGATCGCCAGCCGGTCGATTATGTTATGAACGGACATCAGTATCAGTTACCt                      <  1:1127869/69‑1 (MQ=255)                                           gTGAATGCCACGCATAAAGATCGCCAGCCGGTCGATTATGTTATGAACGGACATCAGTATCAGTTACCt                      <  1:1865874/69‑1 (MQ=255)                                           gTGAATGCCACGCATAAAGATCGCCAGCCGGTCGATTATGTTATGAACGGACATCAGTATCAGTTACCt                      <  1:1825309/69‑1 (MQ=255)                                           gTGAATGCCACGCATAAAGATCGCCAGCCGGTCGATTATGTTATGAACGGACATCAGTATCAGTTACCt                      <  1:3428280/69‑1 (MQ=255)                                           gTGAATGCCACGCATAAAGATCGCCAGCCGGTCGATTATGTTATGAACGGACATCAGTATCAGTTACCt                      <  1:208161/69‑1 (MQ=255)                                           gTGAATGCCACGCATAAAGATCGCCAGCCGGTCGATTATGTTATGAACGGACATCAGTATCAGTTACCt                      <  1:448507/69‑1 (MQ=255)                                           gTGAATGCCACGCATAAAGATCGCCAGCCGGTCGATTATGTTATGAACGGACATCAGTATCAGTTACCt                      <  1:208959/69‑1 (MQ=255)                                           gTGAATGCCACGCATAAAGATCGCCAGCCGGTCGATTATGTTATGAACGGACATCAGTATCAGTTACCt                      <  1:2485623/69‑1 (MQ=255)                                           gTGAATGCCACGCATAAAGATCGCCAGCCGGTCGATTATGTTATGAACGGACATCAGTATCAGTTACCt                      <  1:2576361/69‑1 (MQ=255)                                                         tAAAGATCGCCAGCCGGTCGATTATGTTATGAACGGACATCAGTATCAGTTACCTGATGGCGCTGTGGt        <  1:2689826/69‑1 (MQ=255)                                                         tAAAGATCGCCAGCCGGTCGATTATGTTATGAACGGACATCAGTATCAGTTACCTGATGGCGCTGTGGt        <  1:3035489/69‑1 (MQ=255)                                                         tAAAGATCGCCAGCCGGTCGATTATGTTATGAACGGACATCAGTATCAGTTACCTGATGGCGCTGTGGt        <  1:154510/69‑1 (MQ=255)                                                         tAAAGATCGCCAGCCGGTCGATTATGTTATGAACGGACATCAGTATCAGTTACCTGATGGCGCTGTGGt        <  1:2630418/69‑1 (MQ=255)                                                         tAAAGATCGCCAGCCGGTCGATTATGTTATGAACGGACATCAGTATCAGTTACCTGATGGCGCTGTGGt        <  1:2827749/69‑1 (MQ=255)                                                         tAAAGATCGCCAGCCGGTCGATTATGTTATGAACGGACATCAGTATCAGTTACCTGATGGCGCTGTGGt        <  1:2731012/69‑1 (MQ=255)                                                          aaaGATCGCCAGCCGGTCGATTATGTTATGAACGGACATCAGTATCAGTTACCTGATGGCGCTGTGGt        <  1:1959495/68‑1 (MQ=255)                                                           aaGATCGCCAGCCGGTCGATTATGTTATGCACGGACATCAGTATCAGTTACCTGATGGCGCTGTGGTCa      >  1:200939/1‑69 (MQ=255)                                                           aaGATCGCCAGCCGGTCGATTATGTTATGAACGGACATCAGTATCAGTTAcc                       >  1:1228460/1‑52 (MQ=255)                                                           aaGATCGCCAGCCGGTCGATTATGTTATGAACGGACATCAGTATCAGTTAc                        >  1:1396122/1‑51 (MQ=255)                                                           aaGATCGCCAGCCGGTCGATTATGTTATGAACGGACATCAGTATCAGTTACCTGATg                  >  1:2380525/1‑57 (MQ=255)                                                           aaGATCGCCAGCCGGTCGATTATGTTATGAACGGACATCAGTATCAGTTACCTGATGGCGCTGTGGTc       >  1:2091427/1‑68 (MQ=255)                                                           aaGATCGCCAGCCGGTCGATTATGTTATGAACGGACATCAGTATCAGTTACCTGATGGCGCTGTGGTc       >  1:869153/1‑68 (MQ=255)                                                           aaGATCGCCAGCCGGTCGATTATGTTATGAACGGACATCAGTATCAGTTACCTGATGGCGCTGTGGTCa      >  1:745315/1‑69 (MQ=255)                                                           aaGATCGCCAGCCGGTCGATTATGTTATGAACGGACATCAGTATCAGTTACCTGATGGCGCTGTGGTCa      >  1:1881471/1‑69 (MQ=255)                                                           aaGATCGCCAGCCGGTCGATTATGTTATGAACGGACATCAGTATCAGTTACCTGATGGCGCTGTGGTCa      >  1:2137679/1‑69 (MQ=255)                                                           aaGATCGCCAGCCGGTCGATTATGTTATGAACGGACATCAGTATCAGTTACCTGATGGCGCTGTGGTCa      >  1:2700016/1‑69 (MQ=255)                                                           aaGATCGCCAGCCGGTCGATTATGTTATGAACGGACATCAGTATCAGTTACCTGATGGCGCTGTGGTCa      >  1:1964987/1‑69 (MQ=255)                                                           aaGATCGCCAGCCGGTCGATTATGTTATGAACGGACATCAGTATCAGTTACCTGATGGCGCTGTGGTCa      >  1:1863553/1‑69 (MQ=255)                                                           aaGATCGCCAGCCGGTCGATTATGTTATGAACGGACATCAGTATCAGTTACCTGATGGCGCTGTGGTCa      >  1:2849429/1‑69 (MQ=255)                                                             gATCGCCAGCCGGTCGATTATGTTATGAACGGACATCAGTATCAGTTACCTGATGGCGCTGTGGTCAtt    <  1:2994639/69‑1 (MQ=255)                                                             gATCGCCAGCCGGTCGATTATGTTATGAACGGACATCAGTATCAGTTACCTGATGGCGCTGTGGTCAtt    <  1:593627/69‑1 (MQ=255)                                                              aTCGCCAGCCGGTCGATTATGTTATGAACTGACATCAGTATCAGTTACCTGATGGCGCTGTGGTCAttc   <  1:3294051/69‑2 (MQ=255)                                                              aTCGCCAGCCGGTCGATTATGTTATGAACGGACATCAGTATCAGTTACCTGATGGCGCTGTGGTCAtt    <  1:492343/68‑1 (MQ=255)                                                              aTCGCCAGCCGGTCGATTATGTTATGAACGGACATCAGTATCAGTTACCTGATGGCGCTGTGGTCAtt    <  1:2319748/68‑1 (MQ=255)                                                              aTCGCCAGCCGGTCGATTATGTTATGAACGGACATCAGTATCAGTTACCTGATGGCGCTGTGGTCAtt    <  1:1660664/68‑1 (MQ=255)                                                               tCGCCAGCCGGTCGATTATGTTATGAACGGACATCAGTATCAGTTACCTGATGGCGCTGTGGTCATtgc  <  1:2041881/69‑1 (MQ=255)                                                               tCGCCAGCCGGTCGATTATGTTATGAACGGACATCAGTATCAGTTACCTGATGGCGCTGTGGTCATtgc  <  1:3355249/69‑1 (MQ=255)                                                               tCGCCAGCCGGTCGATTATGTTATGAACGGACATCAGTATCAGTTACCTGATGGCGCTGTGGTCATtgc  <  1:594534/69‑1 (MQ=255)                                                               tCGCCAGCCGGTCGATTATGTTATGAACGGACATCAGTATCAGTTACCTGATGGCGCTGTGGTCATtgc  <  1:2637107/69‑1 (MQ=255)                                                               tCGCCAGCCGGTCGATTATGTTATGAACGGACATCAGTATCAGTTACCTGATGGCGCTGTGGTCATtgc  <  1:2125141/69‑1 (MQ=255)                                                               tCGCCAGCCGGTCGATTATGTTATGAACGGACATCAGTATCAGTTACCTGATGGCGCTGTGGTCATtgc  <  1:1682767/69‑1 (MQ=255)                                                               tCGCCAGCCGGTCGATTATGTTATGAACGGACATCAGTATCAGTTACCTGATGGCGCTGTGGTCATtgc  <  1:2194506/69‑1 (MQ=255)                                                               tCGCCAGCCGGTCGATTATGTTATGAACGGACATCAGTATCAGTTACCTGATGGCGCTGTGGTCATtgc  <  1:1860333/69‑1 (MQ=255)                                                               tCGCCAGCCGGTCGATTATGTTATGAAAGGACATCAGTATCAGTTACCTGATGGCGCTGTGGTCATtgc  <  1:151777/69‑1 (MQ=255)                                                                    |                                                                CCCGATGTACCAAAAGCATTTGCCGCCAGTAACGAACTGGAAGTGAATGCCACGCATAAAGATCGCCAGCCGGTCGATTATGTTATGAACGGACATCAGTATCAGTTACCTGATGGCGCTGTGGTCATTGC  >  minE/875943‑876073

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑