| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 878,242 | G→A | 41.1% | intergenic (‑128/‑42) | ribA ← / → pgpB | GTP cyclohydrolase II/phosphatidylglycerophosphate phosphatase B |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 878,242 | 0 | G | A | 41.1% | 36.1 / 60.0 | 85 | intergenic (‑128/‑42) | ribA/pgpB | GTP cyclohydrolase II/phosphatidylglycerophosphate phosphatase B |
| Reads supporting (aligned to +/- strand): ref base G (22/28); new base A (20/15); total (42/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.74e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01 | |||||||||||
CACCTAATCACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGACTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGC > minE/878175‑878309 | cACCTACTCACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGt > 1:2982773/1‑69 (MQ=255)cACCTAATCACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGt > 1:1690865/1‑69 (MQ=255)cACCTAATCACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGt > 1:1157312/1‑69 (MQ=255)cACCTAATCACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGt > 1:1614842/1‑69 (MQ=255)cACCTAATCACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGt > 1:374951/1‑69 (MQ=255)cACCTAATCACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGt > 1:941145/1‑69 (MQ=255)cACCTAATCACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGt > 1:2633078/1‑69 (MQ=255)cACCTAATCACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGt > 1:3077810/1‑69 (MQ=255) aaTCACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCATTTTCa > 1:3116547/1‑69 (MQ=255) aaTCACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCATTTTCa > 1:3432107/1‑69 (MQ=255) aaTCACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCATTTTCa > 1:3374941/1‑69 (MQ=255) aaTCACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCATTTTCa > 1:2262561/1‑69 (MQ=255) aaTCACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCATTTTCa > 1:3171156/1‑69 (MQ=255) aaTCACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCATTTTCa > 1:3157682/1‑69 (MQ=255) aaTCACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCATTTTCa > 1:431062/1‑69 (MQ=255) aaTCACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCATTTTCa > 1:2543995/1‑69 (MQ=255) aaTCACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCATTTTCa > 1:2630004/1‑69 (MQ=255) aaTCACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCATTTTCa > 1:2615010/1‑69 (MQ=255) aaTCACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCATTTTCa > 1:716476/1‑69 (MQ=255) aaTCACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCATTTTCa > 1:1074082/1‑69 (MQ=255) aaTCACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCATTTTCa > 1:125829/1‑69 (MQ=255) aaTCACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCATTTTCa > 1:918921/1‑69 (MQ=255) aaTCACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCATTTTCa > 1:901540/1‑69 (MQ=255) aaTCACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCATTTTCa > 1:643252/1‑69 (MQ=255) aaTCACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCATTTTCa > 1:606359/1‑69 (MQ=255) aaTCACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCATTTTCa > 1:552110/1‑69 (MQ=255) aaTCACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCATTTTCa > 1:1540579/1‑69 (MQ=255) aaTCACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTACTGGAACTGGCATTTTCa > 1:1326532/1‑69 (MQ=255) cACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGt < 1:3235791/68‑1 (MQ=255) cACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCAttt < 1:985329/63‑1 (MQ=255) cACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCAttt < 1:852558/63‑1 (MQ=255) cACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCAttt < 1:3395152/63‑1 (MQ=255) cACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCAttt < 1:2949553/63‑1 (MQ=255) cACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCAttt < 1:373433/63‑1 (MQ=255) cACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCAttt < 1:1002554/63‑1 (MQ=255) cACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCAttt < 1:526589/63‑1 (MQ=255) cACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCAttt < 1:2331613/63‑1 (MQ=255) cACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCAttt < 1:1537840/63‑1 (MQ=255) cACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCAttt < 1:1524691/63‑1 (MQ=255) cACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCAttt < 1:1515829/63‑1 (MQ=255) cACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCAttt < 1:2344577/63‑1 (MQ=255) cACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCAttt < 1:1263212/63‑1 (MQ=255) acgacgCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGtt > 1:2819622/1‑68 (MQ=255) acgacgCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGtt > 1:1997716/1‑68 (MQ=255) acgacgCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGtt > 1:298235/1‑68 (MQ=255) acgacgCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGtt > 1:3261819/1‑68 (MQ=255) acgacgCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGtt > 1:221527/1‑68 (MQ=255) acgacgCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGt > 1:1554185/1‑67 (MQ=255) acgacgCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCAttt < 1:751334/62‑1 (MQ=255) tCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCAttt < 1:2773184/54‑1 (MQ=255) cGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGACt < 1:3355310/69‑1 (MQ=255) cACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGACTTAt > 1:1390819/1‑68 (MQ=255) cGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGACTTATCa < 1:2365916/68‑1 (MQ=255) aTGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGAGTTATCa < 1:6552/64‑1 (MQ=255) aTGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGACTTATCAAAAAg < 1:657282/69‑1 (MQ=255) aTGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGACTTATCAAAAAg < 1:2603015/69‑1 (MQ=255) aTGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGACTTATCAAAAAg < 1:460326/69‑1 (MQ=255) aTGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGACTTATCAAAAAg < 1:1238870/69‑1 (MQ=255) aTGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGACTTATCAAAAAg < 1:1894799/69‑1 (MQ=255) aTGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGACTTATCAAAAAg < 1:2655142/69‑1 (MQ=255) aTGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGACTTATCAAAAAg < 1:1322501/69‑1 (MQ=255) aTGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGACTTATCAAAAAg < 1:2675697/69‑1 (MQ=255) aTGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGACTTATCAAAAAg < 1:2063964/69‑1 (MQ=255) aTGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGACTTATCAAAAAg < 1:2470295/69‑1 (MQ=255) aTGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGACTTATCAAAAAg < 1:2459815/69‑1 (MQ=255) gCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGACTTATCAAAAAGgag < 1:2031281/69‑1 (MQ=255) gCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGACTTATCAAAAAGgag > 1:2868623/1‑69 (MQ=255) aaaTCTGTCAATTTTTCCTTGAACTGGCGTTTTCAGTTATGATTGTGGGACTTATCAAAAAGGAGAGGc < 1:1408873/69‑1 (MQ=255) aaaTCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGACTTATCAAAAAGGAGAGGc < 1:2529396/69‑1 (MQ=255) aaaTCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGACTTATCAAAAAGGAGAGGc < 1:1740510/69‑1 (MQ=255) aaaTCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGACTTATCAAAAAGGAGAGGc < 1:3372443/69‑1 (MQ=255) aaaTCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGACTTATCAAAAAGGAGAGGc < 1:2449185/69‑1 (MQ=255) aaTCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGACTTATCAAAAAGGAGAGGc < 1:846936/68‑1 (MQ=255) aTCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGACTTATCAAAAAGGAGAGGCCa < 1:585998/69‑1 (MQ=255) aTCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGACTTATCAAAAAGGAGAGGCCa < 1:81765/69‑1 (MQ=255) aTCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGACTTATCAAAAAGGAGAGGCCa < 1:3366366/69‑1 (MQ=255) aaTTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGACTTATCAAAAAGGAGAGGCCATGCGTTc < 1:3412607/69‑1 (MQ=255) tttttCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGACTTATCAAAAAGGAGAGGCCATGCGTTCg < 1:3406823/68‑1 (MQ=255) tttttCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGACTTATCAAAAAGGAGAGGCCATGCGTTCg < 1:2878045/68‑1 (MQ=255) cGTTTTCAGTTATGATTGTGGGACTTATCAAAAAGGAGAGGCCATGCGTTCGATTGcc > 1:2790399/1‑58 (MQ=255) cGTTTTCAGTTATGATTGTGGGACTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGc > 1:1936691/1‑69 (MQ=255) cGTTTTCAGTTATGATTGTGGGACTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGc > 1:703748/1‑69 (MQ=255) cGTTTTCAGTTATGATTGTGGGACTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGc > 1:14263/1‑69 (MQ=255) cGTTTTCAGTTATGATTGTGGGACTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGc > 1:747265/1‑69 (MQ=255) cGTTTTCAGTTATGATTGTGGGACTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGc > 1:2645214/1‑69 (MQ=255) | CACCTAATCACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGACTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGC > minE/878175‑878309 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |