| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 882,776 | T→C | 31.1% | G175G (GGA→GGG) | yciT ← | predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 882,776 | 0 | T | C | 31.1% | 64.4 / 37.5 | 77 | G175G (GGA→GGG) | yciT | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base T (16/37); new base C (18/6); total (34/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.08e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCTTCGCACTCTTTTTCCAGCACGGCATTGACCACATCGGTACGCATCATATCGCGACCGGTAAATCCAGTTTCAGGTTGCCAGCCATCAATACCAATAAATGCTTTGCTGAAATGCACCTGTTGGATGC > minE/882711‑882840 | gCTTCGCACTCTTTTTCCAGCACGGCATTGACCACATCGGTACGCATCATATCGCGACCGGTAAATcc < 1:2412897/68‑1 (MQ=255)gCTTCGCACTCTTTTTCCAGCACGGCATTGACCACATCGGTACGCATCATATCGCGACCGGTAAATcc < 1:3340295/68‑1 (MQ=255) cAGCACGGCATTGACCACATCGGTACGCATCATATCGCGACCGGTAAATCCAGTTTCAGGTTgccagc < 1:1010099/68‑1 (MQ=255) cAGCACGGCATTGACCACATCGGTACGCATCATATCGCGACCGGTAAATCCAGTTTCAGGTTgccagc < 1:784796/68‑1 (MQ=255) cAGCACGGCATTGACCACATCGGTACGCATCATATCGCGACCGGTAAATCCAGTTTCAGGTTgccagc < 1:69574/68‑1 (MQ=255) cAGCACGGCATTGACCACATCGGTACGCATCATATCGCGACCGGTAAATCCAGTTTCAGGTTgccagc < 1:137960/68‑1 (MQ=255) cAGCACGGCATTGACCACATCGGTACGCATCATATCGCGACCGGTAAATCCAGTTTCAGGTTgccagc < 1:2495966/68‑1 (MQ=255) cAGCACGGCATTGACCACATCGGTACGCATCATATCGCGACCGGTAAATCCAGTTTCAGGTTgccagc < 1:459376/68‑1 (MQ=255) cAGCACGGCATTGACCACATCGGTACGCATCATATCGCGACCGGTAAATCCAGTTTCAGGTTgccagc < 1:1514158/68‑1 (MQ=255) cAGCACGGCATTGACCACATCGGTACGCATCATATCGCGACCGGTAAATCCAGTTTCAGGTTgccagc < 1:2326010/68‑1 (MQ=255) cAGCACGGCATTGACCACATCGGTACGCATCATATCGCGACCGGTAAATCCAGTTTCAGGTTgccagc < 1:2923349/68‑1 (MQ=255) cAGCACGGCATTGACCACATCGGTACGCATCATATCGCGACCGGTAAATCCAGTTTCAGGTTgccagc < 1:390475/68‑1 (MQ=255) gCACGGCATTGACCACATCGGTACGCATCATATCGCGACCGGTAAATCCAGTTTCAGGTTgccagc < 1:185564/66‑1 (MQ=255) cACGGCATTGACCACATCGGTACGCATCATATCGCGACCGGTAAATCCAGTTTCAGGTTGCCAGCCATc < 1:3188122/69‑1 (MQ=255) cACGGCATTGACCACAGCGGTACGCATAATATCGCGACCGGTAAATCCAGTTTCAGGTTGCCAGCCATc < 1:582535/69‑1 (MQ=255) gACCACATCGGTACGCATCATATCGCGACCGGTAAAt > 1:1726983/1‑37 (MQ=255) gACCACATCGGTACGCATCATATCGCGACCGGTAAATCCAGTTTCAGGTTGCCAGCCATCAATACCaa > 1:1065515/1‑68 (MQ=255) gACCACATCGGTACGCATCATATCGCGACCGGTAAATCCAGTTTCAGGTTGCCAGCCATCAATACCaa > 1:1697952/1‑68 (MQ=255) gACCACATCGGTACGCATCATATCGCGACCGGTAAATCCAGTTTCAGGTTGCCAGCCATCAATACCaa > 1:31994/1‑68 (MQ=255) gACCACATCGGTACGCATCATATCGCGACCGGTAAATCCAGTTTCAGGTTGCCAGCCATCAATACCaa > 1:1693976/1‑68 (MQ=255) gACCACATCGGTACGCATCATATCGCGACCGGTAAATCCAGTTTCAGGTTGCCAGCCATCAATACCaa > 1:1428836/1‑68 (MQ=255) gACCACATCGGTACGCATCATATCGCGACCGGTAAATCCAGTTTCAGGTTGCCAGCCATCAATACCaa > 1:3180819/1‑68 (MQ=255) gACCACATCGGTACGCATCATATCGCGACCGGTAAATCCAGTTTCAGGTTGCCAGCCATCAATACCaa > 1:1562939/1‑68 (MQ=255) gACCACATCGGTACGCATCATATCGCGACCGGTAAATCCAGTTTCAGGTTGCCAGCCATCAATACCaa > 1:1575209/1‑68 (MQ=255) aTCGGTACGCATCATATCGCGACCGGTAAACCCAGTTTCAGGTTGCCAGCCATCaa > 1:2968298/1‑56 (MQ=255) aTCGGTACGCATCATATCGCGACCGGTAAACCCAGTTTCAGGTTGCCAGCCATCaa > 1:2503880/1‑56 (MQ=255) aTCGGTACGCATCATATCGCGACCGGTAAACCCAGTTTCAGGTTGCCAGCCATCaa > 1:2537289/1‑56 (MQ=255) aTCGGTACGCATCATATCGCGACCGGTAAACCCAGTTTCAGGTTGCCAGCCATCaa > 1:2579015/1‑56 (MQ=255) aTCGGTACGCATCATATCGCGACCGGTAAACCCAGTTTCAGGTTGCCAGCCATCaa > 1:2834711/1‑56 (MQ=255) aTCGGTACGCATCATATCGCGACCGGTAAACCCAGTTTCAGGTTGCCAGCCATCaa > 1:974034/1‑56 (MQ=255) aTCGGTACGCATCATATCGCGACCGGTAAACCCAGTTTCAGGTTGCCAGCCATCaa > 1:3090639/1‑56 (MQ=255) aTCGGTACGCATCATATCGCGACCGGTAAACCCAGTTTCAGGTTGCCAGCCATCaa > 1:3410868/1‑56 (MQ=255) aTCGGTACGCATCATATCGCGACCGGTAAACCCAGTTTCAGGTTGCCAGCCATCaa > 1:3401787/1‑56 (MQ=255) aTCGGTACGCATCATATCGCGACCGGTAAACCCAGTTTCAGGTTGCCAGCCATCaa > 1:238569/1‑56 (MQ=255) aTCGGTACGCATCATATCGCGACCGGTAAACCCAGTTTCAGGTTGCCAGCCATCaa > 1:2390264/1‑56 (MQ=255) aTCGGTACGCATCATATCGCGACCGGTAAACCCAGTTTCAGGTTGCCAGCCATCaa > 1:1757080/1‑56 (MQ=255) aTCGGTACGCATCATATCGCGACCGGTAAACCCAGTTTCAGGTTGCCAGCCATCaa > 1:134211/1‑56 (MQ=255) aTCGGTACGCATCATATCGCGACCGGTAAACCCAGTTTCAGGTTGCCAGCCATCaa > 1:135727/1‑56 (MQ=255) aTCGGTACGCATCATATCGCGACCGGTAAACCCAGTTTCAGGTTGCCAGCCATCaa > 1:2034124/1‑56 (MQ=255) aTCGGTACGCATCATATCGCGACCGGTAAACCCAGTTTCAGGTTGCCAGCCATCaa > 1:1736481/1‑56 (MQ=255) aTCGGTACGCATCATATCGCGACCGGTAAACCCAGTTTCAGGTTGCCAGCCATCaa > 1:1583296/1‑56 (MQ=255) aTCGGTACGCATCATATCGCGACCGGTAAACCCAGTTTAAGGTTGCCAGCCATCaa > 1:3032456/1‑56 (MQ=255) tCGGTACGCATCATATCGCGACCGGTAAATCCAGTTTCAGGTTgccagc < 1:2761269/49‑1 (MQ=255) ggTACGCATCATATCGCGACCGGTAAATCCAGTTTCAGGTTGCCAGCCATCAATACCAATa < 1:2780816/61‑1 (MQ=255) ggTACGCATCATATCGCGACCGGTAAATCCAGTTTCAGGTTGCCAGCCATCAATACCAATa < 1:3428937/61‑1 (MQ=255) ggTACGCATCATATCGCGACCGGTAAATCCAGTTTCAGGTTGCCAGCCATCAATACCAATa < 1:2844548/61‑1 (MQ=255) ggTACGCATCATATCGCGACCGGTAAATCCAGTTTCAGGTTGCCAGCCATCAATACCAATa < 1:2879901/61‑1 (MQ=255) ggTACGCATCATATCGCGACCGGTAAATCCAGTTTCAGGTTGCCAGCCATCAATACCAATa < 1:1692314/61‑1 (MQ=255) ggTACGCATCATATCGCGACCGGTAAATCCAGTTTCAGGTTGCCAGCCATCAATACCAATa < 1:2289034/61‑1 (MQ=255) ggTACGCATCATATCGCGACCGGTAAATCCAGTTTCAGGTTGCCAGCCATCAATACCAATa < 1:859656/61‑1 (MQ=255) ggTACGCATCATATCGCGACCGGTAAATCCAGTTTCAGGTTGCCAGCCATCAATACCAATa < 1:1984243/61‑1 (MQ=255) catcatATCGCGACCGGTAAATCCAGTTTCAGGTTGCCAGCCATCAATACCAATAAATGCTTTGCTGa < 1:1441254/68‑1 (MQ=255) catcatATCGCGACCGGTAAATCCAGTTTCAGGTTGCCAGCCATCAATACCAATAAATGCTTTGCTGa < 1:537119/68‑1 (MQ=255) catcatATCGCGACCGGTAAATCCAGTTTCAGGTTGCCAGCCATCAATACCAATAAATGCTTTGCTGa < 1:1090902/68‑1 (MQ=255) catcatATCGCGACCGGTAAATCCAGTTTCAGGTTGCCAGCCATCAATACCAATAAATGCTTTGCTGa < 1:2417662/68‑1 (MQ=255) catcatATCGCGACCGGTAAATCCAGTTTCAGGTTGCCAGCCATCAATACCAATAAATGCTTTGCTGa < 1:2517098/68‑1 (MQ=255) catcatATCGCGACCGGTAAATCCAGTTTCAGGTTGCCAGCCATCAATACCAATAAATGCTTTGCTGa < 1:2662308/68‑1 (MQ=255) atatCGCGACCGGTAAATCCAGTTTCAGGTTGCCAGCCATCAATACCAATa < 1:421878/51‑1 (MQ=255) atatCGCGACCGGTAAATCCAGTTTCAGGTTGCCAGCCATCAATACCAATAAATGCTTTGCTGAAATGc > 1:2308712/1‑69 (MQ=255) atatCGCGACCGGTAAATCCAGTTTCAGGTTGCCAGCCATCAATACCAATAAATGCTTTGCTGAAATGc > 1:601976/1‑69 (MQ=255) atatCGCGACCGGTAAATCCAGTTTCAGGTTGCCAGCCATCAATACCAATAAATGCTTTGCTGAAATGc > 1:512142/1‑69 (MQ=255) ataaCGCGACCGGTAAATCCAGTTTCAGGTTGCCAGCCATCAATACCAATa < 1:433353/51‑1 (MQ=255) cgcgACCGGTAAATCCAGTTTCAGGTTGCCAGCCATCAATACCAATa < 1:2031669/47‑1 (MQ=255) gcgACCGGTAAATCCAGTTTCAGGTTGCCAGCCATCAATACCAATAAATGCTTTGCTGAAATGCACCTg > 1:237953/1‑69 (MQ=255) gcgACCGGTAAATCCAGTTTCAGGTTGCCAGCCATCAATACCAATAAATGCTTTGCTGAAATGCACCTg > 1:139695/1‑69 (MQ=255) gcgACCGGTAAATCCAGTTTCAGGTTGCCAGCCATCAATACCAATAAATGCTTTGCTGAAATGCACCTg > 1:2971584/1‑69 (MQ=255) gcgACCGGTAAATCCAGTTTCAGGTTGCCAGCCATCAATACCAATAAATGCTTTGCTGAAATGCACCTg > 1:2992283/1‑69 (MQ=255) gcgACCGGTAAATCCAGTTTCAGGTTGCCAGCCATCAATACCAATAAATGCTTTGCTGAAATGCACCTg > 1:3095206/1‑69 (MQ=255) aCCGGTAAATCCAGTTTCAGGTTGCCAGCCATCAATACCAATa < 1:1935022/43‑1 (MQ=255) cGGTAAACCCAGTTTCAGGTTGCCAGCCATCAATACCAATAAATGCTTTGCTGAAATGCACCTGTTGGa < 1:1984387/69‑1 (MQ=255) cGGTAAACCCAGTTTCAGGTTGCCAGCCATCAATACCAATAAATGCTTTGCTGAAATGCACCTGTTGGa < 1:372278/69‑1 (MQ=255) cGGTAAACCCAGTTTCAGGTTGCCAGCCATCAATACCAATAAATGCTTTGCTGAAATGCACCTGTTGGa < 1:2213303/69‑1 (MQ=255) cGGTAAACCCAGTTTCAGGTTGCCAGCCATCAATACCAATAAATGCTTTGCTGAAATGCACCTGTTGGa < 1:2893994/69‑1 (MQ=255) ggTAAACCCAGTTTCAGGTTGCCAGCCATCAATACCAATAAATGCTTTGCTGAAATGCACCTGTTGGa < 1:2441510/68‑1 (MQ=255) ggTAAACCCAGTTTCAGGTTGCCAGCCATCAATACCAATAAATGCTTTGCTGAAATGCACCTGTTGGa < 1:1819615/68‑1 (MQ=255) gTAAATCCAGTTTCAGGTTGCCAGCCATCAATACCAATAAATGCTTTGCTGAAATGCACCTGTTGGATg < 1:2247860/69‑1 (MQ=255) gTAAATCCAGTTTCAGGTTGCCAGCCATCAATACCAATAAATGCTTTGCTGAAATGCACCTGTTGGATg < 1:423510/69‑1 (MQ=255) tAAATCCAGTTTCAGGTTGCCAGCCATCAATACCAATAAATGCTTTGCTGAAATGCACCTGTTGGATGc < 1:2881698/69‑1 (MQ=255) | GCTTCGCACTCTTTTTCCAGCACGGCATTGACCACATCGGTACGCATCATATCGCGACCGGTAAATCCAGTTTCAGGTTGCCAGCCATCAATACCAATAAATGCTTTGCTGAAATGCACCTGTTGGATGC > minE/882711‑882840 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |