| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 889,529 | G→A | 28.2% | N155N (AAC→AAT) | fabI ← | enoyl‑[acyl‑carrier‑protein] reductase, NADH‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 889,529 | 0 | G | A | 28.2% | 138.9 / 54.2 | 127 | N155N (AAC→AAT) | fabI | enoyl‑[acyl‑carrier‑protein] reductase, NADH‑dependent |
| Reads supporting (aligned to +/- strand): ref base G (63/28); new base A (24/12); total (87/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.33e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.06e-01 | |||||||||||
Only 100 of 134 total aligned reads displayed.
GCGTTCGCCATATAGCGCACGTTCGCTTCCAGAGACGCTTTTGCCAGACCCATAACGTTGTAGTTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTCAGCATGGA > minE/889467‑889597 | gcgTTCGCCATATAGCGCACGTTCGCTTCCAGAGACGCTTTTGCCAGACCCATAACGTTGTAGTTCgg > 1:2113487/1‑68 (MQ=255)gcgTTCGCCATATAGCGCACGTTCGCTTCCAGAGACGCTTTTGCCAGACCCATAACGTTGTAGTTCgg > 1:2139649/1‑68 (MQ=255)gcgTTCGCCATATAGCGCACGTTCGCTTCCAGAGACGCTTTTGCCAGACCCATAACGTTGTAGTTCgg > 1:3327944/1‑68 (MQ=255)gcgTTCGCCATATAGCGCACGTTCGCTTCCAGAGACGCTTTTGCCAGACCCATAACGTTGTAGTTCgg > 1:2510018/1‑68 (MQ=255)gcgTTCGCCATATAGCGCACGTTCGCTTCCAGAGACGCTTTTGCCAGACCCATAACGTTGTAGTTCgg > 1:367650/1‑68 (MQ=255)gcgTTCGCCATATAGCGCACGTTCGCTTCCAGAGACGCTTTTGCCAGACCCATAACGTTGTAGTTCgg > 1:23794/1‑68 (MQ=255)gcgTTCGCCATATAGCGCACGTTCGCTTCCAGAGACGCTTTTGCCAGACCCATAACGTTGTAGTTCg > 1:2205618/1‑67 (MQ=255)gcgTTCGCCATATAGCGCACGTTCGCTTCCAGAGACGCTTTTGCCAGACCCATAACGTT‑TAGTTCgg > 1:2748327/1‑67 (MQ=255) taGCGCACGTTCGCTTCGAGAGACGCTTTTGCCAGACCCATAACGTTGTAGTTCGGGATAGCGCGCTCa > 1:712115/1‑69 (MQ=255) taGCGCACGTTCGCTTCCAGAGACGCTTTTGCCAGACCCATAACGTTGTAGTTCGGGa > 1:2621516/1‑58 (MQ=255) taGCGCACGTTCGCTTCCAGAGACGCTTTTGCCAGACCCATAACGTTGTAGTTCGGGa > 1:2135470/1‑58 (MQ=255) taGCGCACGTTCGCTTCCAGAGACGCTTTTGCCAGACCCATAACGTTGTAGTTCGGGa > 1:1875198/1‑58 (MQ=255) taGCGCACGTTCGCTTCCAGAGACGCTTTTGCCAGACCCATAACGTTGTAGTTCGGGa > 1:341977/1‑58 (MQ=255) taGCGCACGTTCGCTTCCAGAGACGCTTTTGCCAGACCCATAACGTTGTAGTTCGGGa > 1:2879609/1‑58 (MQ=255) taGCGCACGTTCGCTTCCAGAGACGCTTTTGCCAGACCCATAACGTTGTAGTTCGGGa > 1:2367535/1‑58 (MQ=255) taGCGCACGTTCGCTTCCAGAGACGCTTTTGCCAGACCCATAACGTTGTAGTTCGGGa > 1:1588754/1‑58 (MQ=255) taGCGCACGTTCGCTTCCAGAGACGCTTTTGCCAGACCCATAACGTTGTAGTTCGGGa > 1:1462897/1‑58 (MQ=255) taGCGCACGTTCGCTTCCAGAGACGCTTTTGCCAGACCCATAACGTTGTAGTTCGGGa > 1:2835451/1‑58 (MQ=255) taGCGCACGTTCGCTTCCAGAGACGCTTTTGCCAGACCCATAACGTTGTAGTTCGGGa > 1:1176390/1‑58 (MQ=255) taGCGCACGTTCGCTTCCAGAGACGCTTTTGCCAGACCCATAACGTTGTAGTTCGGGa > 1:2794966/1‑58 (MQ=255) taGCGCACGTTCGCTTCCAGAGACGCTTTTGCCAGACCCATAACGTTGTAGTTCGGGa > 1:1116476/1‑58 (MQ=255) taGCGCACGTTCGCTTCCAGAGACGCTTTTGCCAGACCCATAACGTTGTAGTTCGGGATAGCGCGCTc > 1:1549620/1‑68 (MQ=255) taGCGCACGTTCGCTTCCAGAGACGCTTTTGCCAGACCCATAACGTTGTAGTTCGGGATAGCGCGCTc > 1:656596/1‑68 (MQ=255) taGCGCACGTTCGCTTCCAGAGACGCTTTTGCCAGACCCATAACGTTGTAGTTCGGGATAGCGCGCTc > 1:3305376/1‑68 (MQ=255) taGCGCACGTTCGCTTCCAGAGACGCTTTTGCCAGACCCATAACGTTGTAGTTCGGGATAGCGCGCTCa > 1:2869783/1‑69 (MQ=255) taGCGCACGTTCGCTTCCAGAGACGCTTTTGCCAGACCCATAACGTTGTAGTTCGGGATAGCGCGCTCa > 1:2460114/1‑69 (MQ=255) taGCGCACGTTCGCTTCCAGAGACGCTTTTGCCAGACCCATAACGTTGTAGTTCGGGATAGCGCGCTCa > 1:1351769/1‑69 (MQ=255) taGCGCACGTTCGCTTCCAGAGACGCTTTTGCCAGACCCATAACGTTGTAGTTCGGGATAGCGCGCTCa > 1:1808591/1‑69 (MQ=255) gTTCGCTTCCAGAGACGCTTTTGCCAGACCCATAACGTTGTAGTTCGGGATAGCGCGCTCAGCGCCaa > 1:2575197/1‑68 (MQ=255) gaCGCTTTTGCCAGACCCATAACGTTGTAGTTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTc > 1:2545406/1‑69 (MQ=255) gaCGCTTTTGCCAGACCCATAACGTTGTAGTTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTc > 1:938005/1‑69 (MQ=255) gaCGCTTTTGCCAGACCCATAACGTTGTAGTTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTc > 1:67369/1‑69 (MQ=255) tAACGTTGTAATTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGa < 1:1039381/68‑1 (MQ=255) tAACGTTGTAATTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGa < 1:1152524/68‑1 (MQ=255) tAACGTTGTAATTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGa < 1:878739/68‑1 (MQ=255) tAACGTTGTAATTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGa < 1:1354352/68‑1 (MQ=255) tAACGTTGTAATTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGa < 1:1424618/68‑1 (MQ=255) tAACGTTGTAATTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGa < 1:1926055/68‑1 (MQ=255) tAACGTTGTAATTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGa < 1:1810958/68‑1 (MQ=255) tAACGTTGTAATTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGa < 1:2906771/68‑1 (MQ=255) tAACGTTGTAATTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGa < 1:3358972/68‑1 (MQ=255) tAACGTTGTAATTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGa < 1:3421759/68‑1 (MQ=255) tAACGTTGTAATTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGa < 1:2866685/68‑1 (MQ=255) tcGTTGTAATTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTc > 1:274504/2‑69 (MQ=255) aCGTTGTAGTTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAgg < 1:1631919/45‑1 (MQ=255) aCGTTGTAGTTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAgg < 1:618101/45‑1 (MQ=255) aCGTTGTAGTTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAgg < 1:3242802/45‑1 (MQ=255) aCGTTGTAGTTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAgg < 1:2003532/45‑1 (MQ=255) aCGTTGTAGTTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAgg < 1:3120017/45‑1 (MQ=255) aCGTTGTAGTTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTc > 1:3209968/1‑69 (MQ=255) aCGTTGTAGTTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTc < 1:3118729/69‑1 (MQ=255) aCGTTGTAGTTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTc > 1:722972/1‑69 (MQ=255) aCGTTGTAGTTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTc < 1:752839/69‑1 (MQ=255) aCGTTGTAGTTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTc < 1:3175201/69‑1 (MQ=255) aCGTTGTAGTTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTc > 1:1027080/1‑69 (MQ=255) aCGTTGTAGTTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTc < 1:3177376/69‑1 (MQ=255) aCGTTGTAGTTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTc > 1:2616391/1‑69 (MQ=255) aCGTTGTAGTTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTc < 1:1303887/69‑1 (MQ=255) aCGTTGTAGTTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTc > 1:1741534/1‑69 (MQ=255) aCGTTGTAGTTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTc > 1:171155/1‑69 (MQ=255) aCGTTGTAGTTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTc > 1:1675357/1‑69 (MQ=255) aCGTTGTAGTTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTc < 1:2069905/69‑1 (MQ=255) aCGTTGTAATTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGTAGGGCAGAACCCGGATTc > 1:2231674/1‑69 (MQ=255) aCGTTGTAATTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTc > 1:1621976/1‑69 (MQ=255) aCGTTGTAATTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTc > 1:3317858/1‑69 (MQ=255) aCGTTGTAATTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTc > 1:3355234/1‑69 (MQ=255) aCGTTGTAATTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTc > 1:3369561/1‑69 (MQ=255) aCGTTGTAATTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTc > 1:1685316/1‑69 (MQ=255) aCGTTGTAATTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTc > 1:1014257/1‑69 (MQ=255) aCGTTGTAATTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTc > 1:624649/1‑69 (MQ=255) aCGTTGTAATTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTc > 1:1502499/1‑69 (MQ=255) aCGTTGTAATTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTc > 1:827734/1‑69 (MQ=255) aCGTTGTAATTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTc > 1:1243757/1‑69 (MQ=255) aCGTTGTAATTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTc > 1:3237427/1‑69 (MQ=255) aCGTTGTAATTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTc > 1:1965849/1‑69 (MQ=255) aCGTTGTAATTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTc > 1:2568033/1‑69 (MQ=255) aCGTTGTAATTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTc > 1:1966922/1‑69 (MQ=255) aCGTTGTAATTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTc > 1:1998768/1‑69 (MQ=255) aCGTTGTAATTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTc > 1:2000061/1‑69 (MQ=255) aCGTTGTAATTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTc > 1:3105820/1‑69 (MQ=255) aCGTTGTAATTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTc > 1:2306912/1‑69 (MQ=255) aCGTTGTAATTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTc > 1:2584071/1‑69 (MQ=255) aCGTTGTAATTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACACGGATTc > 1:478611/1‑69 (MQ=255) gTTGTAGTTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTCAg < 1:468754/69‑1 (MQ=255) gTTGTAGTTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTCAg < 1:1346109/69‑1 (MQ=255) gTTGTAGTTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTCAg < 1:6912/69‑1 (MQ=255) gTTGTAGTTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTCAg < 1:872328/69‑1 (MQ=255) gTTGTAGTTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTCAg < 1:490853/69‑1 (MQ=255) gTTGTAGTTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTCAg < 1:3193455/69‑1 (MQ=255) gTTGTAGTTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTCAg < 1:179115/69‑1 (MQ=255) tAGTTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTCAGCa > 1:1280402/1‑67 (MQ=255) tAGTTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTCAGCa > 1:1874328/1‑67 (MQ=255) tAGTTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTCAGCa > 1:2646116/1‑67 (MQ=255) tAGTTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTCAGCa > 1:1444190/1‑67 (MQ=255) tAGTTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTCAGCa > 1:2149138/1‑67 (MQ=255) tAGTTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTCAGCa > 1:196048/1‑67 (MQ=255) tAGTTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGATCAGCAGGGCAGAACCCGGATTCAGCa > 1:2712522/1‑67 (MQ=255) gTTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAggg > 1:91869/1‑47 (MQ=255) gTTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTCAGCATgg < 1:2272413/68‑1 (MQ=255) gTTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTCAGCATGGa > 1:64606/1‑69 (MQ=255) | GCGTTCGCCATATAGCGCACGTTCGCTTCCAGAGACGCTTTTGCCAGACCCATAACGTTGTAGTTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTCAGCATGGA > minE/889467‑889597 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |