| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 889,789 | C→T | 49.4% | A69T (GCC→ACC) | fabI ← | enoyl‑[acyl‑carrier‑protein] reductase, NADH‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 889,789 | 0 | C | T | 49.4% | 1.5 / 81.3 | 90 | A69T (GCC→ACC) | fabI | enoyl‑[acyl‑carrier‑protein] reductase, NADH‑dependent |
| Reads supporting (aligned to +/- strand): ref base C (24/21); new base T (36/8); total (60/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.28e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.73e-01 | |||||||||||
AGAGTGTACGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGCATCTTCTGCAACATCGCACTGCAGAACGATGTCAGAACCCAATTGAGCGGCAAATTCTTC > minE/889721‑889849 | agagTGTACGAAACCGTCAAATTTCGGCCAAACTTTCCCCTGTTCAGCGAACATGGTGTCGATGCTGGc < 1:2656348/69‑1 (MQ=255)agagTGTACGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGc < 1:724843/69‑1 (MQ=255)agagTGTACGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGc < 1:54315/69‑1 (MQ=255)agagTGTACGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGc < 1:1425368/69‑1 (MQ=255) gagTGTACGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGATCATGGTGTCGATGCTGGCa < 1:2047050/69‑1 (MQ=255) gagTGTACGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGCa < 1:1036114/69‑1 (MQ=255) gagTGTACGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGCa < 1:972896/69‑1 (MQ=255) gagTGTACGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGCa < 1:944910/69‑1 (MQ=255) gagTGTACGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGCa > 1:3364267/1‑69 (MQ=255) gagTGTACGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGCa < 1:3156546/69‑1 (MQ=255) gagTGTACGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGCa < 1:2578801/69‑1 (MQ=255) gagTGTACGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGCa < 1:2123315/69‑1 (MQ=255) gagTGTACGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGCa < 1:1912091/69‑1 (MQ=255) gagTGTACGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGCa < 1:1202392/69‑1 (MQ=255) gagTGTACGAAACCGTCAAAATTCGGCCAAACTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGCa < 1:3336523/69‑1 (MQ=255) agTGTACGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGCa < 1:1130270/68‑1 (MQ=255) tACGAAACCGTCAAATTTCGTCCAAACTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGCAtcttc > 1:822556/1‑69 (MQ=255) tACGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGCAtcttc > 1:3344624/1‑69 (MQ=255) tACGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGCAtcttc > 1:3237611/1‑69 (MQ=255) tACGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGCAtctt > 1:530355/1‑68 (MQ=255) cGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGTAtcttcg > 1:699790/1‑67 (MQ=255) cGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGTAtcttcg > 1:3379284/1‑67 (MQ=255) cGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGTAtcttcg > 1:2882083/1‑67 (MQ=255) cGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGTAtcttcg > 1:2624235/1‑67 (MQ=255) cGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGTAtcttcg > 1:737793/1‑67 (MQ=255) cGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGTAtcttcg > 1:2327337/1‑67 (MQ=255) cGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGTAtcttcg > 1:13093/1‑67 (MQ=255) cGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGTAtcttcg > 1:1115135/1‑67 (MQ=255) cGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGTAtcttcg > 1:1510830/1‑67 (MQ=255) aaCCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGCATCTTCTGCaa < 1:869366/69‑1 (MQ=255) aCCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGCATCTTCTGCAAc < 1:464317/69‑1 (MQ=255) aCCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGCATCTTCTGCAAc < 1:2917772/69‑1 (MQ=255) aaaTTTCGGCCAAACTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGCATCTTCTGCAACATCGCa < 1:2110659/69‑1 (MQ=255) aaaTTTCGGCCAAACTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGCATCTTCTGCAACATCGCa < 1:1006793/69‑1 (MQ=255) aaaTTTCGGCCAAACTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGCATCTTCTGCAACATCGCa < 1:2016248/69‑1 (MQ=255) aaaTTTCGGCCAAACTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGCATCTTCTGCAACATCGCa < 1:352618/69‑1 (MQ=255) aaaTTTCGGCCAAACTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGCATCTTCTGCAACATCGCa < 1:116362/69‑1 (MQ=255) aaaTTTCGGCCAAACTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGCATCTTCTGCAACATCGCa < 1:1896667/69‑1 (MQ=255) ttCGGCCAAACTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGCa < 1:2934252/48‑1 (MQ=255) ccAAACTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGc < 1:850779/42‑1 (MQ=255) aaaCTTTCCCCAGTTCATCGAACATGGTGTCGATGCTGGTATCTTCGGCAACATCGCACTGCAGAACGa > 1:3130281/1‑69 (MQ=255) aaaCTTTCCCCAGTTCAGCGATCATGGTGTCGATGCTGGTATCTTCGGCAACATCGCACTGCAGAACGa > 1:1522266/1‑69 (MQ=255) aaaCTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGTAtc > 1:1680419/1‑43 (MQ=255) aaaCTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGTAtc > 1:3237105/1‑43 (MQ=255) aaaCTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGTATCTTCGGCAACAt > 1:1242833/1‑54 (MQ=255) aaaCTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGTATCTTCGGCAACATCGCACt > 1:276150/1‑60 (MQ=255) aaaCTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGTATCTTCGGCAACATCGCACTTCAGAACGa > 1:2563975/1‑69 (MQ=255) aaaCTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGTATCTTCGGCAACATCGCACTGCAGAACGa < 1:1687085/69‑1 (MQ=255) aaaCTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGTATCTTCGGCAACATCGCACTGCAGAACGa > 1:1708393/1‑69 (MQ=255) aaaCTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGTATCTTCGGCAACATCGCACTGCAGAACGa > 1:3050890/1‑69 (MQ=255) aaaCTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGTATCTTCGGCAACATCGCACTGCAGAACGa > 1:1995353/1‑69 (MQ=255) aaaCTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGTATCTTCGGCAACATCGCACTGCAGAACGa > 1:831865/1‑69 (MQ=255) aaaCTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGTATCTTCGGCAACATCGCACTGCAGAACGa < 1:1236764/69‑1 (MQ=255) aaaCTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGTATCTTCGGCAACATCGCACTGCAGAACGa > 1:867180/1‑69 (MQ=255) aaaCTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGTATCTTCGGCAACATCGCACTGCAGAACGa > 1:2953023/1‑69 (MQ=255) aaaCTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGTATCTTCGGCAACATCGCACTGCAGAACGa > 1:3256744/1‑69 (MQ=255) aaaCTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGTATCTTCGGCAACATCGCACTGCAGAACGa < 1:3330384/69‑1 (MQ=255) aaaCTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGTATCTTCGGCAACATCGCACTGCAGAACGa > 1:164207/1‑69 (MQ=255) aaaCTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGTATCTTCGGCAACATCGCACTGCAGAACGa < 1:3353945/69‑1 (MQ=255) aaaCTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGTATCTTCGGCAACATCGCACTGCAGAACGa > 1:2110739/1‑69 (MQ=255) aaaCTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGTATCTTCGGCAACATCGCACTGCAGAACGa < 1:2363410/69‑1 (MQ=255) aaaCTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGTATCTTCGGCAACATCGCACTGCAGAACGa > 1:2410700/1‑69 (MQ=255) aaaCTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGTATCTTCGGCAACATCGCACTGCAGAACGa < 1:242215/69‑1 (MQ=255) aaaCTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGTATCTTCGGCAACATCGCACTGCAGAACGa > 1:561661/1‑69 (MQ=255) aaaCTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGTATCTTCGGCAACATCGCACTGCAGAACGa < 1:1785693/69‑1 (MQ=255) aaaCTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGTATCTTCGGCAACATCGCACTGCAGAACGa > 1:595883/1‑69 (MQ=255) aaaCTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGTATCTTCGGCAACATCGCACTGCAGAACGa > 1:2850506/1‑69 (MQ=255) aaaCTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGTATCTTCGGCAACATCGCACTGCAGAACGa > 1:2831509/1‑69 (MQ=255) aaaCTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGTATCTTCGGCAACATCGCACTGCAGAACGa > 1:2807076/1‑69 (MQ=255) aaaCTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGTATCTTCGGCAACATCGCACTGCAGAACGa < 1:2671601/69‑1 (MQ=255) aaaCTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGTATCTTCGGCAACATCGCACTGCAGAACGa > 1:2636983/1‑69 (MQ=255) aaaCTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGTATCTTCGGCAACATCGCACTGCAGAACGa > 1:1871937/1‑69 (MQ=255) aaaCTTTCCCCAGTTCAGCGAACATGGTGTCGATGCAGGTATCTTCGGCAACATCGCACTGCAGAACGa > 1:1614268/1‑69 (MQ=255) aaaCTTT‑CCCAGTTCAGCGAACATGGTGTCGATGCTGGTATCTTCGGCAACATCGCACTGCAGAACGAt > 1:1995042/1‑69 (MQ=255) aaaCTTACCCCAGTTCAGCGAACATGGTGTCGATGCTGGTATCTTCGGCAACATCGCACTGCAGAACGa > 1:4953/1‑69 (MQ=255) cTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGCATCTTCTGCAACATCGCACTGCAGAACGAt > 1:1597131/1‑67 (MQ=255) cTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGCATCTTCTGCAACATCGCACTGCAGAACGAt > 1:2073323/1‑67 (MQ=255) cTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGCATCTTCTGCAACATCGCACTGCAGAACGAt > 1:3108377/1‑67 (MQ=255) cTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGCATCTTCTGCAACATCGCACTGCAGAACGAt > 1:2251660/1‑67 (MQ=255) cTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGCATCTTCTGCAACATCGCACTGCAGAACGAt > 1:2936172/1‑67 (MQ=255) cTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGCATCTTCTGCAACATCGCACTGCAGAACGAt > 1:2261933/1‑67 (MQ=255) cTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGCATCTTCTGCAACATCGCACTGCAGAACGAt > 1:2874749/1‑67 (MQ=255) cTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGCATCTTCTGCAACATCGCACTGCAGAACGAt > 1:2325537/1‑67 (MQ=255) cTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGCATCTTCTGCAACATCGCACTGCAGAACGAt > 1:12453/1‑67 (MQ=255) cTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGCATCTTCTGCAACATCGCACTGCAGAACGAt > 1:2501789/1‑67 (MQ=255) tCAGCGAACATGGTGTCGATGCTGGCATCTTCTGCAACAt > 1:3023272/1‑40 (MQ=255) tCAGCGAACATGGTGTCGATGCTGGCATCTTCTGCAACATCGCACTGCATAACGATGTCAGAACCCaa > 1:997852/1‑68 (MQ=255) tCAGCGAACATGGTGTCGATGCTGGCATCTTCTGCAACATCGCACTGCAGAACGATGTCAGAACCCaa > 1:1466271/1‑68 (MQ=255) cAGCGAACATGGTTTCGATGCTGTGATCTTCTGCAACATGGCa < 1:2899841/43‑1 (MQ=25) gATGCTGGCATCTTCTGCAACATCGCACTGCAGAACGATGTCAGAACCCAATTGAGCGGCAAAttcttc > 1:2474899/1‑69 (MQ=255) gATGCTGGCATCTTCTGCAACATCGCACTGCAGAACGATGTCAGAACCCAATTGAGCGGCAAAttcttc > 1:2601049/1‑69 (MQ=255) gATGCTGGCATCTTCTGCAACATCGCACTGCAGAACGATGTCAGAACCCAATTGAGCGGCAAAttcttc > 1:296156/1‑69 (MQ=255) gATGCTGGCATCTTCTGCAACATCGCACTGCAGAACGATGTCAGAACCCAATTGAGCGGCAAAttcttc > 1:2078304/1‑69 (MQ=255) gATGCTGGCATCTTCTGCAACATCGCACTGCAGAACGATGTCAGAACCCAATTGAGCGGCAAAttcttc > 1:3226571/1‑69 (MQ=255) gATGCTGGCATCTTCTGCAACATCGCACTGCAGAACGATGTCAGAACCCAATTGAGCGGCAAAttcttc > 1:3435165/1‑69 (MQ=255) | AGAGTGTACGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGCATCTTCTGCAACATCGCACTGCAGAACGATGTCAGAACCCAATTGAGCGGCAAATTCTTC > minE/889721‑889849 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |