| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 891,127 | T→C | 37.3% | G154G (GGA→GGG) | sapF ← | predicted antimicrobial peptide transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 891,127 | 0 | T | C | 37.3% | 28.9 / 36.1 | 59 | G154G (GGA→GGG) | sapF | predicted antimicrobial peptide transporter subunit |
| Reads supporting (aligned to +/- strand): ref base T (15/22); new base C (5/17); total (20/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.55e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.97e-01 | |||||||||||
TTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCAT > minE/891061‑891189 | ttCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGccc < 1:1078189/69‑1 (MQ=255)ttCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGccc < 1:1829871/69‑1 (MQ=255)ttCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGccc < 1:1444968/69‑1 (MQ=255)ttCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGccc < 1:2655185/69‑1 (MQ=255)ttCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGccc < 1:1358428/69‑1 (MQ=255)ttCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGccc < 1:1082078/69‑1 (MQ=255) ataatGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACGCAGACGCTGTTTTTGCCCGGGTGCTAAc < 1:1759805/69‑1 (MQ=255) ataatGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGCCCGGGTGCTAAc < 1:691947/69‑1 (MQ=255) ataatGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGCCCGGGTGCTAAc < 1:528612/69‑1 (MQ=255) ataatGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGCCCGGGTGCTAAc < 1:3199875/69‑1 (MQ=255) ataatGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGCCCGGGTGCTAAc < 1:202248/69‑1 (MQ=255) ataatGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGCCCGGGTGCTAAc < 1:1009602/69‑1 (MQ=255) ataatGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGCCCGGGTGCTAAc < 1:1120834/69‑1 (MQ=255) ataatGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGCCCGGGTGCTAAc < 1:2624491/69‑1 (MQ=255) ataatGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGCCCGGGTGCTAAc < 1:2393781/69‑1 (MQ=255) ataatGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACACAGACGCTGTTTTTGCCCGGGTGCTAAc < 1:2785350/69‑1 (MQ=255) taatGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACa < 1:654236/69‑1 (MQ=255) taatGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACa < 1:144028/69‑1 (MQ=255) taatGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACa < 1:135431/69‑1 (MQ=255) taatGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACa < 1:1338967/69‑1 (MQ=255) taatGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGCCCGGGTGCTAAc < 1:1887612/68‑1 (MQ=255) aatGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACat > 1:2446006/1‑69 (MQ=255) aatGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACat > 1:2513947/1‑69 (MQ=255) aatGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACat > 1:84543/1‑69 (MQ=255) aatGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACat > 1:1218344/1‑69 (MQ=255) aatGACTTTTGGGCCCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACat > 1:3319989/1‑69 (MQ=255) atGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACata < 1:1908445/69‑1 (MQ=255) atGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACata < 1:534694/69‑1 (MQ=255) atGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACata > 1:1397968/1‑69 (MQ=255) atGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACat > 1:1169067/1‑68 (MQ=255) tttGGGCGCAATATCAGCTCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGa < 1:3244083/69‑1 (MQ=255) tttGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGa < 1:1308071/69‑1 (MQ=255) tttGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGa < 1:749808/69‑1 (MQ=255) tttGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGa < 1:1173222/69‑1 (MQ=255) tttGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGa < 1:3207814/69‑1 (MQ=255) tttGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGa < 1:1331132/69‑1 (MQ=255) tttGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGa < 1:1992712/69‑1 (MQ=255) gcgcAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGATAGt < 1:1492844/68‑1 (MQ=255) gcgcAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGATAGt < 1:2103818/68‑1 (MQ=255) cAATATCGGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACt < 1:578571/69‑1 (MQ=255) cAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACt < 1:1224908/69‑1 (MQ=255) cAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACt < 1:2457419/69‑1 (MQ=255) atatCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGa > 1:3092266/1‑69 (MQ=255) atatCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGa > 1:1633100/1‑69 (MQ=255) atatCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGa > 1:514984/1‑69 (MQ=255) atatCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGa > 1:3307869/1‑69 (MQ=255) tCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGt > 1:1841128/1‑69 (MQ=255) tCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGt > 1:711889/1‑69 (MQ=255) tCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGt > 1:3093324/1‑69 (MQ=255) tCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGt > 1:2151661/1‑69 (MQ=255) cgcgcgcgcCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACata < 1:41012/46‑1 (MQ=255) cgcgcgcgcCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGa < 1:2956091/52‑1 (MQ=255) ccagacccagacGCTGTTTTTGCCCGGGTGCTAACATATGCGGATAGTAACTGACGTGATCCGGTagca < 1:1665104/69‑1 (MQ=255) ccagacccagacGCTGTTTTTGCCCGGGTGCTAACATATGCGGATAGTAACTGACGTGATCCGGTagca < 1:76054/69‑1 (MQ=255) ccagacccagacGCTGTTTTTGCCCGGGGGCTAACATATGCGGATAGTAACTGACGTGATCCGGTagca < 1:1390962/69‑1 (MQ=255) cagacccagacGCTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTagcag < 1:528389/69‑1 (MQ=255) cagacccagacGCTGTTTTTGCCCGGGTGCTAACATATGCGGATAGTAACTGACGTGATCCGGTagca < 1:1880784/68‑1 (MQ=255) cagacccagacGCTGTTTTTGCCCGGGTGCTAACATATGCGGATAGTAACTGACGTGATCCGGTagca < 1:3028326/68‑1 (MQ=255) acGCTGTTTTTGCCCGGGTGCTAACATATGCGGATAGTAACTGACGTGATCCGGTagca < 1:795635/59‑1 (MQ=255) gTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTagcag < 1:421891/55‑1 (MQ=255) tttttGCCCGGGTGCTAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCa > 1:466426/1‑68 (MQ=255) tttttGCCCGGGTGCTAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCAt > 1:3260738/1‑69 (MQ=255) tttttGCCCGGGTGCTAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCAt > 1:2201290/1‑69 (MQ=255) tttttGCCCGGGTGCTAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCAt > 1:13751/1‑69 (MQ=255) tttttGCCAGGGTGCTAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCAt > 1:2049549/1‑69 (MQ=255) | TTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCAT > minE/891061‑891189 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |