| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 892,843 | T→G | 21.6% | P210P (CCA→CCC) | sapC ← | predicted antimicrobial peptide transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 892,843 | 0 | T | G | 21.6% | 130.3 / 38.7 | 88 | P210P (CCA→CCC) | sapC | predicted antimicrobial peptide transporter subunit |
| Reads supporting (aligned to +/- strand): ref base T (19/50); new base G (15/4); total (34/54) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.30e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.05e-01 | |||||||||||
GGCGATATCGAGAATTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGACGGGCGGCGATAACGTACTC > minE/892777‑892911 | ggcgATATCGAGAATTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTgg < 1:381973/69‑1 (MQ=255) atCGAGAATTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTgg < 1:161701/63‑1 (MQ=255) atCGAGAATTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTgg < 1:2248800/63‑1 (MQ=255) atCGAGAATTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTgg < 1:2271852/63‑1 (MQ=255) atCGAGAATTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTgg < 1:1131722/63‑1 (MQ=255) tCGAGAATTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGGGATGTTTgg < 1:593389/62‑1 (MQ=255) gaATTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCa < 1:2421709/68‑1 (MQ=255) gaATTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCa < 1:612801/68‑1 (MQ=255) gCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGGATCACAGCAAAc > 1:1119272/1‑66 (MQ=255) gCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAAc > 1:2666440/1‑66 (MQ=255) gCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAAc > 1:2334949/1‑66 (MQ=255) gCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAAc > 1:3107049/1‑66 (MQ=255) aTCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCagag < 1:65169/68‑1 (MQ=255) aTCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCagag < 1:2745115/68‑1 (MQ=255) aTCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCagag < 1:1837187/68‑1 (MQ=255) tCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGaa < 1:2371846/69‑1 (MQ=255) tCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGaa < 1:870467/69‑1 (MQ=255) tCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGaa < 1:397920/69‑1 (MQ=255) gACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAata < 1:918883/69‑1 (MQ=255) gACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAata < 1:3346013/69‑1 (MQ=255) gACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAata < 1:2719020/69‑1 (MQ=255) gcgcGGGTGATCTCGGTGCCCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGc > 1:1855753/1‑69 (MQ=255) gcgcGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGc > 1:2906796/1‑69 (MQ=255) gcgcGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGc > 1:500662/1‑69 (MQ=255) gcgcGGGTGATCTAGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGc > 1:160338/1‑69 (MQ=255) cgGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTgg < 1:2761912/38‑1 (MQ=255) cgGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGtt < 1:999822/69‑1 (MQ=255) cgGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGtt < 1:2528077/69‑1 (MQ=255) cgGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGtt < 1:2856702/69‑1 (MQ=255) cgGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGtt < 1:2929446/69‑1 (MQ=255) cgGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGtt < 1:3076568/69‑1 (MQ=255) cgGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGtt < 1:1041056/69‑1 (MQ=255) cgGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGtt < 1:328100/69‑1 (MQ=255) cgGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGtt < 1:631477/69‑1 (MQ=255) cgGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGtt < 1:1784199/69‑1 (MQ=255) cgGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGtt < 1:1066855/69‑1 (MQ=255) gggTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGtt < 1:3289337/68‑1 (MQ=255) tGATCTCGGTGACCAGCCCGGCGTTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGtt < 1:1086176/65‑1 (MQ=255) tGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGtt < 1:1156054/65‑1 (MQ=255) tGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGtt < 1:3376758/65‑1 (MQ=255) tGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGtt < 1:2911285/65‑1 (MQ=255) tGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGtt < 1:38194/65‑1 (MQ=255) tGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGtt < 1:3367913/65‑1 (MQ=255) tGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGtt < 1:576289/65‑1 (MQ=255) tGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGtt < 1:3008233/65‑1 (MQ=255) tGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGtt < 1:2732297/65‑1 (MQ=255) tGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGtt < 1:961690/65‑1 (MQ=255) tGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGtt < 1:3081380/65‑1 (MQ=255) tGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGtt < 1:649632/65‑1 (MQ=255) tGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGtt < 1:1980841/65‑1 (MQ=255) tGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGtt < 1:79285/65‑1 (MQ=255) tGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGtt < 1:2066516/65‑1 (MQ=255) gATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGtt < 1:1468654/64‑1 (MQ=255) gATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGtt < 1:2955404/64‑1 (MQ=255) gATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGtt < 1:1675991/64‑1 (MQ=255) cGGTGACCAGTCCGGCGGTGATGTTGGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCa > 1:1223775/1‑68 (MQ=255) cGGTGACCAGTCCGGCGGTGATGTTGGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCa > 1:1209525/1‑68 (MQ=255) cGGTGACCAGTCCGGCGGTGATGTTGGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCa > 1:3342113/1‑68 (MQ=255) cGGTGACCAGTCCGGCGGTGATGTTGGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCa > 1:643202/1‑68 (MQ=255) cGGTGACCAGTCCGGCGGTGATGTTGGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCa > 1:3337878/1‑68 (MQ=255) cGGTGACCAGTCCGGCGGTGATGTTGGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCa > 1:981511/1‑68 (MQ=255) cGGTGACCAGTCCGGCGGTGATGTTGGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCa > 1:1027800/1‑68 (MQ=255) cGGTGACCAGTCCGGCGGTGATGTTGGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCa > 1:218724/1‑68 (MQ=255) cGGTGACCAGTCCGGCGGTGATGTTGGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCa > 1:1575077/1‑68 (MQ=255) cGGTGACCAGTCCGGCGGTGATGTTGGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCa > 1:2046891/1‑68 (MQ=255) cGGTGACCAGTCCGGCGGTGATGTTGGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCa > 1:2973052/1‑68 (MQ=255) cGGTGACCAGTCCGGCGGTGATGTTGGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCa > 1:1663326/1‑68 (MQ=255) cGGTGACCAGTCCGGCGGTGATGTTGGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCa > 1:1816381/1‑68 (MQ=255) cGGTGACCAGTCCGGCGGTGATGTTGCGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCa > 1:870727/1‑68 (MQ=255) cGGTGACCAGTCCGGCGGTGATGTTGCGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCa > 1:1784185/1‑68 (MQ=255) ggTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCa > 1:2143992/1‑67 (MQ=255) ggTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCa > 1:3405688/1‑67 (MQ=255) ggTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCa > 1:1779976/1‑67 (MQ=255) cAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGACg > 1:1775711/1‑69 (MQ=255) aGCCCGGCTGTTATGTTTTGAATCACAGCAAACCAGAGAATATTCAGCGtt < 1:1340988/51‑1 (MQ=37) ccGGCGGTGATGTTGGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGACGggcg < 1:412224/69‑1 (MQ=255) ccGGCGGTGATGTTGGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGACGggcg < 1:2769438/69‑1 (MQ=255) ccGGCGGTGATGTTGGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGACGggcg < 1:403530/69‑1 (MQ=255) cggcggTGATGTTGGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGACGggcg < 1:2215435/68‑1 (MQ=255) gcggTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGACGggcggc > 1:3216355/1‑68 (MQ=255) gcggTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGACGggcggc > 1:886378/1‑68 (MQ=255) gcggTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGACGggcggc > 1:720264/1‑68 (MQ=255) tGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGtt < 1:3253746/41‑1 (MQ=255) gATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGtt < 1:12725/40‑1 (MQ=255) gATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGACGGGCGGCGATaa > 1:1938368/1‑68 (MQ=255) gATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGACGGGCGGCGATaa > 1:1790523/1‑68 (MQ=255) gATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGACGGGCGGCGATaa > 1:1149835/1‑68 (MQ=255) gATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGACGGGCGGCGATaa > 1:1290370/1‑68 (MQ=255) tGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATgcgc > 1:1727678/1‑41 (MQ=255) tGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGACGGGCGGCGATAACGTActc > 1:2232329/1‑69 (MQ=255) tGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGACGGGCGGCGATAACGTActc > 1:1654845/1‑69 (MQ=255) tGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGACGGGCGGCGATAACGTActc > 1:1060915/1‑69 (MQ=255) tGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGACGGGCGGCGATAACGTActc > 1:3248945/1‑69 (MQ=255) | GGCGATATCGAGAATTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGACGGGCGGCGATAACGTACTC > minE/892777‑892911 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |