| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 893,176 | C→T | 44.0% | V99V (GTG→GTA) | sapC ← | predicted antimicrobial peptide transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 893,176 | 0 | C | T | 44.0% | 5.3 / 22.5 | 34 | V99V (GTG→GTA) | sapC | predicted antimicrobial peptide transporter subunit |
| Reads supporting (aligned to +/- strand): ref base C (7/12); new base T (5/10); total (12/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGA > minE/893116‑893243 | gAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCGAGCGTCACCACAAATGCGCCACCTACGGTTgg < 1:1270881/69‑1 (MQ=255)gAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCGAGCGTCACCACAAATGCGCCACCTACGGTTgg < 1:3044971/69‑1 (MQ=255)gAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCGAGCGTCACCACAAATGCGCCACCTACGGTTgg < 1:2873648/69‑1 (MQ=255)gAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCGAGCGTCACCACAAATGCGCCACCTACGGTTgg < 1:2585514/69‑1 (MQ=255)gAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCGAGCGTCACCACAAATGCGCCACCTACGGTTgg < 1:2575829/69‑1 (MQ=255)gAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCGAGCGTCACCACAAATGCGCCACCTACGGTTgg < 1:2394180/69‑1 (MQ=255)gAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCGAGCGTCACCACAAATGCGCCACCTACGGTTgg < 1:1740117/69‑1 (MQ=255)gAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCGAGCGTCACCACAAATGCGCCACCTACGGTTgg < 1:1643909/69‑1 (MQ=255)gAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCGAGCGTCACCACAAATGCGCCACCTACGGTTgg < 1:1623462/69‑1 (MQ=255)gAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCGAGCCTCACCACAAATGCGCCACCTACGGTTgg < 1:55419/69‑1 (MQ=255) cccAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCt > 1:1739508/1‑69 (MQ=255) cccAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCt > 1:599685/1‑69 (MQ=255) cccAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCt > 1:1887895/1‑69 (MQ=255) cccAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCt > 1:3131188/1‑69 (MQ=255) ccAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTc > 1:400411/1‑68 (MQ=255) ccAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTCGCGCAGCTCCGCt > 1:2660924/1‑67 (MQ=255) gcgGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACa < 1:975907/69‑1 (MQ=255) gcgGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACa < 1:33871/69‑1 (MQ=255) gcgGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACa < 1:16191/69‑1 (MQ=255) gcgGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACa < 1:2071530/69‑1 (MQ=255) cgGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACa < 1:806690/68‑1 (MQ=255) cgGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACa < 1:703968/68‑1 (MQ=255) cgGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACa < 1:56693/68‑1 (MQ=255) ggCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACa < 1:75497/67‑1 (MQ=255) ggCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACa < 1:1364191/67‑1 (MQ=255) aGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGt < 1:1607543/69‑1 (MQ=255) aGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGt < 1:1763125/69‑1 (MQ=255) aGCGTCACCACAAATGAGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGt < 1:2770525/69‑1 (MQ=255) cGTCACCACAAATGCGCCACCTACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGt > 1:213030/1‑67 (MQ=255) cGTCACCACAAATGCGCCACCTACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGt > 1:1836895/1‑67 (MQ=255) cGTCACCACAAATGCGCCACCTACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGt > 1:1240798/1‑67 (MQ=255) cGTCACCACAAATGCGCCACCTACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGt > 1:2757288/1‑67 (MQ=255) cGTCACCACAAATGCGCCACCTACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGt > 1:1483132/1‑67 (MQ=255) accacAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAgg > 1:759980/1‑69 (MQ=255) ccacccacGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTccc < 1:891398/68‑1 (MQ=255) ccacccacGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTccc < 1:896977/68‑1 (MQ=255) ccacccacGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTccc < 1:2319915/68‑1 (MQ=255) cacGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTc > 1:2509081/1‑47 (MQ=255) cacGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAgg > 1:3015638/1‑67 (MQ=255) cacGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGga > 1:2226743/1‑68 (MQ=255) cacGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGga > 1:1862249/1‑68 (MQ=255) cacGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGga > 1:540297/1‑68 (MQ=255) cacGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGga > 1:3003721/1‑68 (MQ=255) cacGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGga > 1:2142238/1‑68 (MQ=255) cacGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGga > 1:1608712/1‑68 (MQ=255) | GAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGA > minE/893116‑893243 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |