| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 15,001 | C→T | 27.2% | G278G (GGC→GGT) | dnaJ → | chaperone Hsp40, co‑chaperone with DnaK |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 15,001 | 0 | C | T | 27.2% | 93.9 / 32.3 | 84 | G278G (GGC→GGT) | dnaJ | chaperone Hsp40, co‑chaperone with DnaK |
| Reads supporting (aligned to +/- strand): ref base C (37/24); new base T (8/15); total (45/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.93e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCGTGAAGGCAACAACCTGTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTA > minE/14938‑15068 | gCGTGAAGGCAACAACCTGTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGTGAAAt < 1:1217574/69‑1 (MQ=255)gCGTGAAGGCAACAACCTGTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGTGAAAt < 1:2972140/69‑1 (MQ=255)gCGTGAAGGCAACAACCTGTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGTGAAAt < 1:2265323/69‑1 (MQ=255)gCGTGAAGGCAACAACCTGTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGTGAAAt < 1:392671/69‑1 (MQ=255)gCGTGAAGGCAACAACCTGTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGTGAAAt < 1:491239/69‑1 (MQ=255)gCGTGAAGGCAACAACCTGTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGTGAAAt < 1:501025/69‑1 (MQ=255)gCGTGAAGGCAACAACCTGTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGTGAAAt < 1:1688161/69‑1 (MQ=255)gCGTGAAGGCAACAACCTGTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGTGAAAt < 1:171623/69‑1 (MQ=255)gCGTGAAGGCAACAACCTGTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGTGAAAt < 1:1745573/69‑1 (MQ=255)gCGTGAAGGCAACAACCTGTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGTGAAAt < 1:1748598/69‑1 (MQ=255)gCGTGAAGGCAACAACCTGTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGTGAAAt < 1:254453/69‑1 (MQ=255)gCGTGAAGGCAACAACCTGTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGTGAAAt < 1:1858185/69‑1 (MQ=255) cGTGAAGGCAACAACCTGTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGTGAAAt < 1:2496195/68‑1 (MQ=255) gTGAAGGCAACAACCTGTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCg < 1:893372/69‑1 (MQ=255) gTGAAGGCAACAACCTGTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCg < 1:2747855/69‑1 (MQ=255) gTGAAGGCAACAACCTGTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCg < 1:2793712/69‑1 (MQ=255) gTGAAGGCAACAACCTGTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCg < 1:1787178/69‑1 (MQ=255) gTGAAGGCAACAACCTGTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCg < 1:756985/69‑1 (MQ=255) gTGAAGGCAACAACCTGTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCg < 1:753976/69‑1 (MQ=255) gTGAAGGCAACAACCTGTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCg < 1:1142446/69‑1 (MQ=255) gTGAAGGCAACAACCTGTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCg < 1:1347844/69‑1 (MQ=255) gTGAAGGCAACAACCTGTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCg < 1:562683/69‑1 (MQ=255) gTGAAGGCAACAACCTGTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCg < 1:718259/69‑1 (MQ=255) caacaaCCTGTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTAcc > 1:1925068/1‑69 (MQ=255) caacaaCCTGTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTAcc > 1:760087/1‑69 (MQ=255) caacaaCCTGTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTAcc > 1:571407/1‑69 (MQ=255) caacaaCCTGTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTAcc > 1:3298105/1‑69 (MQ=255) acCTGTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGTGAAAt < 1:1771347/55‑1 (MQ=255) cTGTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCtt < 1:1715210/69‑1 (MQ=255) cTGTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCtt < 1:422625/69‑1 (MQ=255) cTGTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCtt < 1:2842312/69‑1 (MQ=255) tGTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTg < 1:1899344/69‑1 (MQ=255) tGTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTg < 1:3143612/69‑1 (MQ=255) gTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGTGAAATCGAAGTACCGACCCTTGa > 1:1063428/1‑69 (MQ=255) gTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGTGAAATCGAAGTACCGACCCTTGa > 1:2251059/1‑69 (MQ=255) gTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGTGAAATCGAAGTACCGACCCTTGa > 1:2681873/1‑69 (MQ=255) gTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGTGAAATCGAAGTACCGACCCTTGa > 1:2852426/1‑69 (MQ=255) gTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGTGAAATCGAAGTACCGACCCTTGa > 1:2905160/1‑69 (MQ=255) gTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGTGAAATCGAAGTACCGACCCTTGa > 1:1445345/1‑69 (MQ=255) gTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGTGAAATCGAAGTACCGACCCTTGa > 1:1277523/1‑69 (MQ=255) gTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGTGAAATCGAAGTACCGACCCTTGa > 1:1680870/1‑69 (MQ=255) aTTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGTGAAAt < 1:502928/49‑1 (MQ=255) ttGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTa > 1:1587985/1‑55 (MQ=255) ttGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGa > 1:878537/1‑66 (MQ=255) ttGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATgg > 1:2564636/1‑69 (MQ=255) ttGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATgg > 1:171322/1‑69 (MQ=255) ttGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATgg > 1:2922479/1‑69 (MQ=255) ttGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATgg > 1:1120894/1‑69 (MQ=255) ttGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATgg > 1:1645337/1‑69 (MQ=255) ttGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATgg > 1:1641139/1‑69 (MQ=255) ttGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATgg > 1:2877147/1‑69 (MQ=255) ccGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCaaa > 1:939125/1‑69 (MQ=255) ccGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCaaa > 1:785211/1‑69 (MQ=255) ccGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCaa > 1:3350918/1‑68 (MQ=255) ccGATCAACTTCGCTATGGCGGCGCTGGGTGGCAAAATCGAAGTACCGACCCTTGATGGTCGCGTCaaa > 1:242676/1‑69 (MQ=255) cAACTTCTCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGaa < 1:1500608/69‑1 (MQ=255) cAACTTCTCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGaa < 1:1496606/69‑1 (MQ=255) cAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCaa > 1:1124751/1‑63 (MQ=255) cAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCaa > 1:164714/1‑63 (MQ=255) cAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCaa > 1:271812/1‑63 (MQ=255) cAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCaa > 1:741959/1‑63 (MQ=255) cAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCaa > 1:118443/1‑63 (MQ=255) cAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCaa > 1:1575838/1‑63 (MQ=255) cAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCaa > 1:322980/1‑63 (MQ=255) cAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCaa > 1:3375018/1‑63 (MQ=255) cAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCaa > 1:489505/1‑63 (MQ=255) cAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGaa < 1:868381/69‑1 (MQ=255) cAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGaa < 1:3317125/69‑1 (MQ=255) cAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGaa < 1:493484/69‑1 (MQ=255) cAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGaa < 1:3036955/69‑1 (MQ=255) cAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAACTACCGACCCTTGATGGTCGCGTCAAACTGaa > 1:908744/1‑69 (MQ=255) ttCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTg < 1:2980189/69‑1 (MQ=255) ttCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTg < 1:1119893/69‑1 (MQ=255) cGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGcc > 1:1174226/1‑69 (MQ=255) cGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGcc > 1:1857833/1‑69 (MQ=255) cGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGcc > 1:2097580/1‑69 (MQ=255) gcggcgCTGGGTGGCGAAATCGAAGTACCGCCCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGaa > 1:2235690/1‑69 (MQ=255) gcggcgCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGaa > 1:1372759/1‑69 (MQ=255) gcggcgCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGaa > 1:1615589/1‑69 (MQ=255) gcggcgCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGaa > 1:280608/1‑69 (MQ=255) gcggcgCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGaa > 1:1954639/1‑69 (MQ=255) gggCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTg < 1:1345646/48‑1 (MQ=255) gCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTa > 1:2896317/1‑69 (MQ=255) gCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTa > 1:105456/1‑69 (MQ=255) | GCGTGAAGGCAACAACCTGTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTA > minE/14938‑15068 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |