| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 83,694 | G→A | 31.1% | V184V (GTG→GTA) | ftsW → | integral membrane protein involved in stabilising FstZ ring during cell division |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 83,694 | 0 | G | A | 31.1% | 87.1 / 44.2 | 93 | V184V (GTG→GTA) | ftsW | integral membrane protein involved in stabilising FstZ ring during cell division |
| Reads supporting (aligned to +/- strand): ref base G (13/51); new base A (16/13); total (29/64) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.42e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01 | |||||||||||
CTATCTGGTGCGTAAAGGCGACGAAGTACGTAATAACCTGCGCGGCTTCCTGAAACCGATGGGCGTGATTCTGGTGTTGGCAGTGTTACTGCTGGCACAGCCAGACCTTGGTACGGTGGTGGTGTTGTTTGT > minE/83628‑83759 | cTATCTGGTGCGTAAAGGCGACGAAGTACGTAATAACCTGCGCGGCTTCCTGAAACCGATGGGCGTGAt < 1:3426497/69‑1 (MQ=255)cTATCTGGTGCGTAAAGGCGACGAAGTACGTAATAACCTGCGCGGCTTCCTGAAACCGATGGGCGTGAt < 1:3212628/69‑1 (MQ=255)cTATCTGGTGCGTAAAGGCGACGAAGTACGTAATAACCTGCGCGGCTTCCTGAAACCGATGGGCGTGAt < 1:2417961/69‑1 (MQ=255)cTATCTGGTGCGTAAAGGCGACGAAGTACGTAATAACCTGCGCGGCTTCCTGAAACCGATGGGCGTGAt < 1:1587080/69‑1 (MQ=255)cTATCTGGTGCGTAAAGGCGACGAAGTACGTAATAACCTGCGCGGCTTCCTGAAACCGATGGGCGTGAt < 1:982686/69‑1 (MQ=255) tATCTGGTGCGTAAAGGCGACGAAGTACGTAATAACCTGCGCGGCTTCCTGAAACCGATGGGCGTGAtt > 1:2425292/1‑69 (MQ=255) tATCTGGTGCGTAAAGGCGACGAAGTACGTAATAACCTGCGCGGCTTCCTGAAACCGATGGGCGTGAt > 1:2617732/1‑68 (MQ=255) tGGTGCGTAAAGGCGACGAAGTACGTAATAACCTGCGCGGCTTCCTGAAACCGATGGGCGTAATTCTg < 1:102394/68‑1 (MQ=255) tGGTGCGTAAAGGCGACGAAGTACGTAATAACCTGCGCGGCTTCCTGAAACCGATGGGCGTAATTCTg < 1:3383124/68‑1 (MQ=255) tGGTGCGTAAAGGCGACGAAGTACGTAATAACCTGCGCGGCTTCCTGAAACCGATGGGCGTAATTCTg < 1:3279966/68‑1 (MQ=255) tGGTGCGTAAAGGCGACGAAGTACGTAATAACCTGCGCGGCTTCCTGAAACCGATGGGCGTAATTCTg < 1:3248239/68‑1 (MQ=255) tGGTGCGTAAAGGCGACGAAGTACGTAATAACCTGCGCGGCTTCCTGAAACCGATGGGCGTAATTCTg < 1:419091/68‑1 (MQ=255) tGGTGCGTAAAGGCGACGAAGTACGTAATAACCTGCGCGGCTTCCTGAAACCGATGGGCGTAATTCTg < 1:2842773/68‑1 (MQ=255) tGGTGCGTAAAGGCGACGAAGTACGTAATAACCTGCGCGGCTTCCTGAAACCGATGGGCGTAATTCTg < 1:2777749/68‑1 (MQ=255) tGGTGCGTAAAGGCGACGAAGTACGTAATAACCTGCGCGGCTTCCTGAAACCGATGGGCGTAATTCTg < 1:531276/68‑1 (MQ=255) tGGTGCGTAAAGGCGACGAAGTACGTAATAACCTGCGCGGCTTCCTGAAACCGATGGGCGTAATTCTg < 1:2623797/68‑1 (MQ=255) tGGTGCGTAAAGGCGACGAAGTACGTAATAACCTGCGCGGCTTCCTGAAACCGATGGGCGTAATTCTg < 1:2483811/68‑1 (MQ=255) tGGTGCGTAAAGGCGACGAAGTACGTAATAACCTGCGCGGCTTCCTGAAACCGATGGGCGTAATTCTg < 1:110258/68‑1 (MQ=255) tGGTGCGTAAAGGCGACGAAGTACGTAATAACCTGCGCGGCTTCCTGAAACCGATGGGCGTAATTCTg < 1:1889598/68‑1 (MQ=255) gCGTAAAGGCGACGAAGTACGTAATAACCTGCGCGGCTTCCTGAAACCGATGTGCGTGATTCTGgt < 1:192588/66‑1 (MQ=255) gCGTAAAGGCGACGAAGTACGTAATAACCTGCGCGGCTTCCTGAAACCGATGGGCGTGATTCTgg < 1:2556807/65‑1 (MQ=255) gCGTAAAGGCGACGAAGTACGTAATAACCTGCGCGGCTTCCTGAAACCGATGGGCGTGATTCTgg < 1:2713196/65‑1 (MQ=255) gCGTAAAGGCGACGAAGTACGTAATAACCTGCGCGGCTTCCTGAAACCGATGGGCGTGATTCTgg < 1:705306/65‑1 (MQ=255) gCGTAAAGGCGACGAAGTACGTAATAACCTGCGCGGCTTCCTGAAACCGATGGGCGTGATTCTGgt < 1:2792252/66‑1 (MQ=255) gCGTAAAGGCGACGAAGTACGTAATAACCTGCGCGGCTTCCTGAAACCGATGGGCGTGATTCTGgt < 1:1795893/66‑1 (MQ=255) gCGTAAAGGCGACGAAGTACGTAATAACCTGCGCGGCTTCCTGAAACCGATGGGCGTGATTCTGgt < 1:1602821/66‑1 (MQ=255) gCGTAAAGGCGACGAAGTACGTAATAACCTGCGCGGCTTCCTGAAACCGATGGGCGTGATTCTGgt < 1:1943121/66‑1 (MQ=255) gCGTAAAGGCGACGAAGTACGTAATAACCTGCGCGGCTTCCTGAAACCGATGGGCGTGATTCTGGTGtt > 1:103583/1‑69 (MQ=255) gCGTAAAGGCGACGAAGTACGTAATAACCTGCGCGGCTTCCTGAAACCGATGGGCGTGATTCTGGTGtt > 1:588326/1‑69 (MQ=255) aaGGCGACGAAGTACGTAATAACCTGCGCGGCTTCCTGAAACCGATGGGCGTGATTCTGGTGTTGGCAg < 1:1660789/69‑1 (MQ=255) aaGGCGACGAAGTACGTAATAACCTGCGCGGCTTCCTGAAACCGATGGGCGTGATTCTGGTGTTGGCAg < 1:2376137/69‑1 (MQ=255) gCGACGAAGTACGTAATAACCTGCGCGGCTTCCTGAAACCGATGGGCGTGATTCTGGTGTTGGCAgtgt < 1:2333450/69‑1 (MQ=255) gCGACGAAGTACGTAATAACCTGCGCGGCTTCCTGAAACCGATGGGCGTGATTCTGGTGTTGGCAgtgt < 1:1219594/69‑1 (MQ=255) gCGACGAAGTACGTAATAACCTGCGCGGCTTCCTGAAACCGATGGGCGTGATTCTGGTGTTGGCAgtgt < 1:2991144/69‑1 (MQ=255) gCGACGAAGTACGTAATAACCTGCGCGGCTTCCTGAAACCGATGGGCGTGATTCTGGTGTTGGCAgtgt < 1:2913704/69‑1 (MQ=255) gCGACGAAGTACGTAATAACCTGCGCGGCTTCCTGAAACCGATGGGCGTGATTCTGGTGTTGGCAgtgt < 1:1310816/69‑1 (MQ=255) gCGACGAAGTACGTAATAACCTGCGCGGCTTCCTGAAACCGATGGGCGTGATTCTGGTGTTGGCAgtgt < 1:1770570/69‑1 (MQ=255) cgaAGTACGTAATAACCTGCGCGGCTTCCTGAAACCGATGGGCGTGATTCTGGTGTTGGCAGTGTTAct < 1:466998/69‑1 (MQ=255) cgaAGTACGTAATAACCTGCGCGGCTTCCTGAAACCGATGGGCGTAATTCTGGTGtt > 1:2922251/1‑57 (MQ=255) cgaAGTACGTAATAACCTGCGCGGCTTCCTGAAACCGATGGGCGTAATTCTGGTGTTGGCAGTGTTAct > 1:3328577/1‑69 (MQ=255) cgaAGTACGTAATAACCTGCGCGGCTTCCTGAAACCGATGGGCGTAATTCTGGTGTTGGCAGTGTTAct > 1:3201900/1‑69 (MQ=255) cgaAGTACGTAATAACCTGCGCGGCTTCCTGAAACCGATGGGCGTAATTCTGGTGTTGGCAGTGTTAct > 1:3123836/1‑69 (MQ=255) cgaAGTACGTAATAACCTGCGCGGCTTCCTGAAACCGATGGGCGTAATTCTGGTGTTGGCAGTGTTAct > 1:3078373/1‑69 (MQ=255) cgaAGTACGTAATAACCTGCGCGGCTTCCTGAAACCGATGGGCGTAATTCTGGTGTTGGCAGTGTTAct > 1:3019221/1‑69 (MQ=255) cgaAGTACGTAATAACCTGCGCGGCTTCCTGAAACCGATGGGCGTAATTCTGGTGTTGGCAGTGTTAct > 1:1660104/1‑69 (MQ=255) cgaAGTACGTAATAACCTGCGCGGCTTCCTGAAACCGATGGGCGTAATTCTGGTGTTGGCAGTGTTAct > 1:2897014/1‑69 (MQ=255) cgaAGTACGTAATAACCTGCGCGGCTTCCTGAAACCGATGGGCGTAATTCTGGTGTTGGCAGTGTTAct > 1:2809999/1‑69 (MQ=255) cgaAGTACGTAATAACCTGCGCGGCTTCCTGAAACCGATGGGCGTAATTCTGGTGTTGGCAGTGTTAct > 1:1520368/1‑69 (MQ=255) cgaAGTACGTAATAACCTGCGCGGCTTCCTGAAACCGATGGGCGTAATTCTGGTGTTGGCAGTGTTAct > 1:352486/1‑69 (MQ=255) cgaAGTACGTAATAACCTGCGCGGCTTCCTGAAACCGATGGGCGTAATTCTGGTGTTGGCAGTGTTAct > 1:3354003/1‑69 (MQ=255) cgaAGTACGTAATAACCTGCGCGGCTTCCTGAAACCGATGGGCGTAATTCTGGTGTTGGCAGTGTTAct > 1:1170190/1‑69 (MQ=255) cgaAGTACGTAATAACCTGCGCGGCTTCCTGAAACCGATGGGCGTAATTCTGGTGTTGGCAGTGTTAct > 1:685668/1‑69 (MQ=255) cgaAGTACGTAATAACCTGCGCGGCTTCCTGAAACCGATGGGCGTAATTCTGGTGTTGGCAGTGTTAct > 1:538110/1‑69 (MQ=255) cgaAGTACGTAATAACCTGCGCGGCTTCCTGAAACCGATGGGCGTAATTCTGGTGTTGGCAGTGTTAct > 1:1071303/1‑69 (MQ=255) aGTACGTAATAACCTGCGCGGCTTCCTGAAACCGATGGGCGTAATTCTg < 1:535107/49‑1 (MQ=255) gTACGTAATAACCTGCGCGGCTTCCTGAAACCGATGTGCGTGAt < 1:2601185/44‑1 (MQ=255) gTACGTAATAACCTGCGCGGCTTCCTGAAACCGATGGGCGTGAt < 1:3317678/44‑1 (MQ=255) gTACGTAATAACCTGCGCGGCTTCCTGAAACCGATGGGCGTGAt < 1:1378666/44‑1 (MQ=255) gTACGTAATAACCTGCGCGGCTTCCTGAAACCGATGGGCGTGAt < 1:3426335/44‑1 (MQ=255) gTACGTAATAACCTGCGCGGCTTCCTGAAACCGATGGGCGTGAt < 1:2325853/44‑1 (MQ=255) gTACGTAATAACCTGCGCGGCTTCCTGAAACCGATGGGCGTGAt < 1:2173515/44‑1 (MQ=255) gTACGTAATAACCTGCGCGGCTTCCTGAAACCGATGGGCGTGATTCTGGTGTTGGc > 1:2228352/1‑56 (MQ=255) tACGTAATAACCTGCGCGGCTTCCTGAAACCTATGGGCGTGAt < 1:1856804/43‑1 (MQ=255) aaCCTGCGCGGCTTCCTGAAACCGATGGGCGTGATTCTGGTGTTGGCAGTGTTACTGCTGGCACAGCCa < 1:3198012/69‑1 (MQ=255) aaCCTGCGCGGCTTCCTGAAACCGATGGGCGTGATTCTGGTGTTGGCAGTGTTACTGCTGGCACAGCCa < 1:2915630/69‑1 (MQ=255) aaCCTGCGCGGCTTCCTGAAACCGATGGGCGTGATTCTGGTGTTGGCAGTGTTACTGCTGGCACAGCCa < 1:489911/69‑1 (MQ=255) aaCCTGCGCGGCTTCCTGAAACCGATGGGCGTGATTCTGGTGTTGGCAGTGTTACTGCTGGCACAGCCa < 1:1243884/69‑1 (MQ=255) aaCCTGCGCGGCTTCCTGAAACCGATGGGCGTGATTCTGGTGTTGGCAGTGTTACTGCTGGCACAGCCa < 1:1239246/69‑1 (MQ=255) aaCCTGCGCGGCTTCCTGAAACCGATGGGCGTGATTCTGGTGTTGGCAGTGTTACTGCTGGCACAGCCa > 1:1704087/1‑69 (MQ=255) aaCCTGCGCGGCTTCCTGAAACCGATGGGCGTGATTCTGGTGTTGGCAGTGTTACTGCTGGCACAGCCa < 1:1495625/69‑1 (MQ=255) aaCCTGCGCGGCTTCCTGAAACCGATGGGCGTGATTCTGGTGTTGGCAGTGTTACTGCTGGCACAGCCa < 1:1531007/69‑1 (MQ=255) aaCCTGCGCGGCTTCCTGAAACCGATGGGCGTGATTCTGGTGTTGGCAGTGTTACTGCTGGCACAGCCa < 1:892781/69‑1 (MQ=255) aCCTGCGCGGCTTCCTGAAACCGATGGGCGTGATTCTGGTGTTGGCAGTGTTACTGCTGGCACAGCCa > 1:1530881/1‑68 (MQ=255) cgcgGCTTCCTGAAACCGATGGGCGTGATTCTGGTGtt > 1:425008/1‑38 (MQ=255) cgcgGCTTCCTGAAACCGATGGGCGTGATTCTGGTGtt > 1:1035816/1‑38 (MQ=255) cgcgGCTTCCTGAAACCGATGGGCGTGATTCTGGTGtt > 1:3330823/1‑38 (MQ=255) cgcgGCTTCCTGAAACCGATGGGCGTGATTCTGGTGtt > 1:1212535/1‑38 (MQ=255) cgcgGCTTCCTGAAACCGATGGGCGTGATTCTGGTGtt > 1:2448682/1‑38 (MQ=255) cgcgGCTTCCTGAAACCGATGGGCGTGATTCTGGTGtt > 1:1493712/1‑38 (MQ=255) cgGCTTCCTGAAACCGATGGGCGTGATTCTGGTGTTGGCAGTGTTACTGCTGGCACAGCCAGACCTTgg < 1:435421/69‑1 (MQ=255) cgGCTTCCTGAAACCGATGGGCGTGATTCTGGTGTTGGCAGTGTTACTGCTGGCACAGCCAGACCTTgg < 1:842210/69‑1 (MQ=255) ggCTTCCTGAAACCGATGGGCGTGATTCTGGTGTTGGCAGTGTTACTGCTGGCACAGCCAGACCTTgg < 1:3163986/68‑1 (MQ=255) ttCCTGAAACCGATGGGCGTGATTCTGGTGTTGGCAgtgt < 1:3247294/40‑1 (MQ=255) ttCCTGAAACCGATGGGCGTGATTCTGGTGTTGGCAgtgt < 1:2832931/40‑1 (MQ=255) ttCCTGAAACCGATGGGCGTGATTCTGGTGTTGGCAgtgt < 1:2716956/40‑1 (MQ=255) ttCCTGAAACCGATGGGCGTGATTCTGGTGTTGGCAgtgt < 1:58749/40‑1 (MQ=255) ttCCTGAAACCGATGGGCGTGATTCTGGTGTTGGCAgtgt < 1:1639684/40‑1 (MQ=255) ttCCTGAAACCGATGGGCGTGATTCTGGTGTTGGCAgtgt < 1:838939/40‑1 (MQ=255) cGTGATTCTGGTGTTGGCAGTGTTACTGCTGGCACAGCCAGACCTTGGTACGGTGGTGGTGttgtttgt < 1:1113959/69‑1 (MQ=255) cGTGATTCTGGTGTTGGCAGTGTTACTGCTGGCACAGCCAGACCTTGGTACGGTGGTGGTGttgtttgt < 1:1549517/69‑1 (MQ=255) cGTGATTCTGGTGTTGGCAGTGTTACTGCTGGCACAGCCAGACCTTGGTACGGTGGTGGTGttgtttgt < 1:2616324/69‑1 (MQ=255) cGTGATTCTGGTGTTGGCAGTGTTACTGCTGGCACAGCCAGACCTTGGTACGGTGGTGGTGttgtttgt < 1:2854508/69‑1 (MQ=255) cGTGATTCTGGTGTTGGCAGTGTTACTGCTGGCACAGCCAGACCTTGGTACGGTGGTGGTGttgtttgt < 1:360385/69‑1 (MQ=255) | CTATCTGGTGCGTAAAGGCGACGAAGTACGTAATAACCTGCGCGGCTTCCTGAAACCGATGGGCGTGATTCTGGTGTTGGCAGTGTTACTGCTGGCACAGCCAGACCTTGGTACGGTGGTGGTGTTGTTTGT > minE/83628‑83759 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |