| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 893,899 | T→C | 50.4% | I176V (ATC→GTC) | sapB ← | predicted antimicrobial peptide transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 893,899 | 0 | T | C | 50.4% | ‑2.6 / 119.3 | 125 | I176V (ATC→GTC) | sapB | predicted antimicrobial peptide transporter subunit |
| Reads supporting (aligned to +/- strand): ref base T (44/18); new base C (44/19); total (88/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.69e-01 | |||||||||||
Only 100 of 125 total aligned reads displayed.
TCAGTTGTTGGCGCAACCGACAGGGTGATCACGGGCAATATCATATGGCGGATTGCGCTCATGATCATTTCATCCCGCCATGGTGAGTCCGAAAGCCAGGCATCAATCAACGCAAAACCGGTAATCGGTTTC > minE/893835‑893966 | cctgttgttGGCGCAACCGACAGGGTGATCACGGGCAATATCATATGGCGGATTGCGCTCATGACCAtt < 1:1590106/66‑1 (MQ=255) gttgttGGCGCAACCGACAGGGTGATCACGGGCAATATCATATGGCGGATTGCGCTCATGATCAttt < 1:86173/67‑1 (MQ=255) gttgttGGCGCAACCGACAGGGTGATCACGGGCAATATCATATGGCGGATTGCGCTCATGATCAttt < 1:2865179/67‑1 (MQ=255) tttgGCGCAACCGACAGGGTGATCACGGGCAATATCATATGGCGGATTGCGCTCATGACCAtt < 1:2385534/62‑1 (MQ=255) gttgGCGCAACCTACAGGGTGATCACGGGCAATATCATATGGCGGATTGCGCTCATGACCAtt < 1:2061645/63‑1 (MQ=255) gttgGCGCAACCGACAGGGTGATCACGGGCAATCTCATATGGCGGATTGCGCTCATGACCAtt < 1:2202555/63‑1 (MQ=255) gttgGCGCAACCGACAGGGTGATCACGGGCAATATCATATGGCGGATTGCGCTCATGACCAtt < 1:1035360/63‑1 (MQ=255) gttgGCGCAACCGACAGGGTGATCACGGGCAATATCATATGGCGGATTGCGCTCATGACCAtt < 1:2108576/63‑1 (MQ=255) gttgGCGCAACCGACAGGGTGATCACGGGCAATATCATATGGCGGATTGCGCTCATGACCAtt < 1:1914043/63‑1 (MQ=255) gttgGCGCAACCGACAGGGTGATCACGGGCAATATCATATGGCGGATTGCGCTCATGACCAtt < 1:2499725/63‑1 (MQ=255) gttgGCGCAACCGACAGGGTGATCACGGGCAATATCATATGGCGGATTGCGCTCATGACCAtt < 1:172091/63‑1 (MQ=255) gttgGCGCAACCGACAGGGTGATCACGGGCAATATCATATGGCGGATTGCGCTCATGACCAtt < 1:2746171/63‑1 (MQ=255) gttgGCGCAACCGACAGGGTGATCACGGGCAATATCATATGGCGGATTGCGCTCATGACCAtt < 1:2749409/63‑1 (MQ=255) gttgGCGCAACCGACAGGGTGATCACGGGCAATATCATATGGCGGATTGCGCTCATGACCAtt < 1:1473505/63‑1 (MQ=255) gttgGCGCAACCGACAGGGTGATCACGGGCAATATCATATGGCGGATTGCGCTCATGACCAtt < 1:3259341/63‑1 (MQ=255) gttgGCGCAACCGACAGGGTGATCACGGGCAATATCATATGGCGGATTGCGCTCATGACCAtt < 1:133529/63‑1 (MQ=255) gttgGCGCAACCGACAGGGTGATCACGGGCAATATCATATGGCGGATTGCGCTCATGACCAtt < 1:742303/63‑1 (MQ=255) gttgGCGCAACCGACAGGGTGATCACGGGCAATATCATATGGCGGATTGCGCTAATGACCAtt < 1:1024714/63‑1 (MQ=255) tgGCGCAACCGACAGGGTGATCACGGGCAATATCATATGGCGGATTGCGCTCATGACCATTTCATccc > 1:2677619/1‑68 (MQ=255) tgGCGCAACCGACAGGGTGATCACGGGCAATATCATATGGCGGATTGCGCTCATGACCATTTCATccc > 1:903438/1‑68 (MQ=255) tgGCGCAACCGACAGGGTGATCACGGGCAATATCATATGGCGGATTGCGCTCATGACCATTTCATccc > 1:2153844/1‑68 (MQ=255) tgGCGCAACCGACAGGGTGATCACGGGCAATATCATATGGCGGATTGCGCTCATGACCATTTCATccc > 1:805457/1‑68 (MQ=255) tgGCGCAACCGACAGGGTGATCACGGGCAATATCATATGGCGGATTGCGCTCATGACCATTTCATccc > 1:2292300/1‑68 (MQ=255) tgGCGCAACCGACAGGGTGATCACGGGCAATATCATATGGCGGATTGCGCTCATGACCATTTCATccc > 1:2352340/1‑68 (MQ=255) tgGCGCAACCGACAGGGTGATCACGGGCAATATCATATGGCGGATTGCGCTCATGACCATTTCATccc > 1:2381332/1‑68 (MQ=255) tgGCGCAACCGACAGGGTGATCACGGGCAATATCATATGGCGGATTGCGCTCATGACCATTTCATccc > 1:3397760/1‑68 (MQ=255) tgGCGCAACCGACAGGGTGATCACGGGCAATATCATATGGCGGATTGCGCTCATGACCATTTCATccc > 1:3319837/1‑68 (MQ=255) tgGCGCAACCGACAGGGTGATCACGGGCAATATCATATGGCGGATTGCGCTCATGACCATTTCATccc > 1:3109508/1‑68 (MQ=255) tgGCGCAACCGACAGGGTGATCACGGGCAATATCATATGGCGGATTGCGCTCATGACCATTTCATccc > 1:2737836/1‑68 (MQ=255) tgGCGCAACCGACAGGGTGATCACGGGCAATATCATATGGCGGATTGCGCTCATGACCATTTCATccc > 1:3310527/1‑68 (MQ=255) tgGCGCAACCGACAGGGTGATCACGGGCAATATCATATGGCGGATTGCGCTCATGACCATTTCATccc > 1:3273006/1‑68 (MQ=255) tgGCGCAACCGACAGGGTGATCACGGGCAATATCATATGGCGGATTGCGCTCATGACCATTTCATccc > 1:3261346/1‑68 (MQ=255) tgGCGCAACCGACAGGGTGATCACGGGCAATATCATATGGCGGATTGCGCTCATGACCATTTCATccc > 1:3018005/1‑68 (MQ=255) tgGCGCAACCGACAGGGTGATCACGGGCAATATCATATGGCGGATTGCGCTCATGACCATTTCATccc > 1:3108336/1‑68 (MQ=255) tgGCGCAACCGACAGGGTGATCACGGGCAATATCATATGGCGGATTGCGCTCATGACCATTTCATccc > 1:3147769/1‑68 (MQ=255) tgGCGCAACCGACAGGGTGATCACGGGCAATATCATATGGCGGATTGCGCTCATGACCATTTCATccc > 1:1038755/1‑68 (MQ=255) tgGCGCAACCGACAGGGTGATCACGGGCAATATCATATGGCGGATTGCGCTCATGACCATTTCATccc > 1:1674944/1‑68 (MQ=255) tgGCGCAACCGACAGGGTGATCACGGGCAATATCATATGGCGGATTGCGCTCATGACCATTTCATccc > 1:1702951/1‑68 (MQ=255) tgGCGCAACCGACAGGGTGATCACGGGCAATATCATATGGCGGATTGCGCTCATGACCATTTCATccc > 1:1539645/1‑68 (MQ=255) tgGCGCAACCGACAGGGTGATCACGGGCAATATCATATGGCGGATTGCGCTCATGACCATTTCATccc > 1:1801049/1‑68 (MQ=255) tgGCGCAACCGACAGGGTGATCACGGGCAATATCATATGGCGGATTGCGCTCATGACCATTTCATccc > 1:1804948/1‑68 (MQ=255) cgcAACCGACAGGGTGATCACGGGCAATATCATATGGCGGATTGCGCTCATGATCATTTCATCCCGcc < 1:3344023/68‑1 (MQ=255) cgcAACCGACAGGGTGATCACGGGCAATATCATATGGCGGATTGCGCTCATGATCATTTCATCCCGcc < 1:1448055/68‑1 (MQ=255) cgcAACCGACAGGGTGATCACGGGCAATATCATATGGCGGATTGCGCTCATGATCATTTCATCCCGcc < 1:292789/68‑1 (MQ=255) gACAGGGTGATCACGGGCAATATCATATGGCGGATTGCGCTCATGACCATTTCATCCCGCCa > 1:1882774/1‑62 (MQ=255) gACAGGGTGATCACGGGCAATATCATATGGCGGATTGCGCTCATGACCATTTCATCCCGCCACGGt > 1:236484/1‑66 (MQ=255) gACAGGGTGATCACGGGCAATATCATATGGCGGATTGCGCTCATGACCATTTCATCCCGCCACGGt > 1:1929654/1‑66 (MQ=255) gACAGGGTGATCACGGGCAATATCATATGGCGGATTGCGCTCATGACCATTTCATCCCGCCACGGt > 1:2536554/1‑66 (MQ=255) gACAGGGTGATCACGGGCAATATCATATGGCGGATTGCGCTCATGACCATTTCATCCCGCCACGGt > 1:3335169/1‑66 (MQ=255) gACAGGGTGATCACGGGCAATATCATATGGCGGATTGCGCTCATGACCATTTCATCCCGCCACGGt > 1:1416945/1‑66 (MQ=255) gACAGGGTGATCACGGGCAATATCATATGGCGGATTGCGCTCATGACCATTTCATCCCGCCACGGt > 1:2671738/1‑66 (MQ=255) gACAGGGTGATCACGGGCAATATCATATGGCGGATTGCGCTCATGACCATTTCATCCCGCCACGGt > 1:1799268/1‑66 (MQ=255) gACAGGGTGATCACGGGCAATATCATATGGCGGATTGCGCTCATGACCATTTCATCCCGCCACGGt > 1:2739618/1‑66 (MQ=255) gACAGGGTGATCACGGGCAATATCATATGGCGGATTGCGCTCATGACCATTTCATCCCGCCACGGt > 1:704596/1‑66 (MQ=255) gACAGGCTGATCACGGGCAATATCATATGGCGGATTGCGCTCATGACCATTTCATCCCGCCACGGt > 1:439922/1‑66 (MQ=255) cACGGGCAATATCATATGGCGGATTGCGCTCATGATCATTTCATCCCGCCATGGTGAGTCCGAAAGcc > 1:2062204/1‑68 (MQ=255) cACGGGCAATATCATATGGCGGATTGCGCTCATGATCATTTCATCCCGCCATGGTGAGTCCGAAAGCCa > 1:2142137/1‑69 (MQ=255) cACGGGCAATATCATATGGCGGATTGCGCTCATGATCATTTCATCCCGCCATGGTGAGTCCGAAAGCCa > 1:2581015/1‑69 (MQ=255) tCATATGGCGGATTGCGCTCATGATCATTTCATCCCGCCATGGt < 1:133284/44‑1 (MQ=255) tCATATGGCGGATTGCGCTCATGATCATTTCATCCCGCCATGGt < 1:3362054/44‑1 (MQ=255) tCATATGGCGGATTGCGCTCATGATCATTTCATCCCGCCATGGt < 1:713077/44‑1 (MQ=255) tCATATGGCGGATTGCGCTCATGATCATTTCATCCCGCCATGGTGAGTCCGAAAGCCAGGCatcaatca > 1:1200808/1‑69 (MQ=255) tCATATGGCGGATTGCGCTCATGATCATTTCATCCCGCCATGGTGAGTCCGAAAGCCAGGCatcaatca > 1:2634654/1‑69 (MQ=255) tCATATGGCGGATTGCGCTCATGATCATTTCATCCCGCCATGGTGAGTCCGAAAGCCAGGCatcaatca > 1:1163594/1‑69 (MQ=255) tCATATGGCGGATTGCGCTCATGATCATTTCATCCCGCCATGGTGAGTCCGAAAGCCAGGCatcaatca > 1:303768/1‑69 (MQ=255) tCATATGGCGGATTGCGCTCATGATCATTTCATCCCGCCATGGTGAGTCCGAAAGCCAGGCatcaatca > 1:3019453/1‑69 (MQ=255) tCATATGGCGGATTGCGCTCATGATCATTTCATCCCGCCATGGTGAGTCCGAAAGCCAGGCatcaatca > 1:193666/1‑69 (MQ=255) tCATATGGCGGATTGCGCTCATGATCATTTCATCCCGCCATGGTGAGTCCGAAAGCCAGGCatcaatca > 1:1043354/1‑69 (MQ=255) tCATATGGCGGATTGCGCTCATGATCATTTCATCCCGCCATGGTGAGTCCGAAAGCCAGGCatcaatca > 1:2774593/1‑69 (MQ=255) tCATATGGCGGATTGCGCTCATGATCATTTCATCCCGCCATGGTGAGTCCGAAAGCCAGGCatcaatca > 1:894504/1‑69 (MQ=255) atatGGCGGATTGCGCTCATGACCATTTCATCCCGCCACGGTGAGTTCGAAAGCCAGTCAtcaatcaa > 1:701557/1‑68 (MQ=255) atatGGCGGATTGCGCTCATGACCATTTCATCCCGCCACGGTGAGTTCGAAAGCCAGGCAtcaatcaa > 1:567733/1‑68 (MQ=255) atatGGCGGATTGCGCTCATGACCATTTCATCCCGCCACGGTGAGTTCGAAAGCCAGGCAtcaatcaa > 1:1316430/1‑68 (MQ=255) atGGCGGATTGCGCTCATGATCATTTCATCCCGCCATGGTGAGTCCGAAAGCCAGGCATCAATCAACg > 1:1142080/1‑68 (MQ=255) gCGGATTGCGCTCATGATCATTTCATCCCGCCATGGTGAGTCCGAAAGCCAGGCATCAATCAACGCaaa > 1:728915/1‑69 (MQ=255) gCGGATTGCGCTCATGATCATTTCATCCCGCCATGGTGAGTCCGAAAGCCAGGCATCAATCAACGCaaa > 1:2771453/1‑69 (MQ=255) gCGGATTGCGCTCATGATCATTTCATCCCGCCATGGTGAGTCCGAAAGCCAGGCATCAATCAACGCaaa > 1:2691/1‑69 (MQ=255) ttGCGCTCATGATCATTTCATCCCGCCATGGTGAGTCCGAAAGCCAGGCATCAATCAACGCAAAACCg > 1:2576839/1‑68 (MQ=255) tGCGCTCATGATCATTTCATCCCTCCATGGTGAGTCCGAAAGCCAGGCAtcaatcaa > 1:511678/1‑57 (MQ=255) tGCGCTCATGATCATTTCATCCCGCCATGGTGAGTCCGAAAGCCAGGCATCAATCAACGCAAAACCGGt > 1:2389373/1‑69 (MQ=255) tGCGCTCATGATCATTTCATCCCGCCATGGTGAGTCCGAAAGCCAGGCATCAATCAACGCAAAACCGGt < 1:36504/69‑1 (MQ=255) tGCGCTCATGATCATTTCATCCCGCCATGGTGAGTCCGAAAGCCAGGCATCAATCAACGCAAAACCGGt < 1:45394/69‑1 (MQ=255) cATGATCATTTCATCCCGCCATGGTGAGTCCGAAAGCCAGGCATCAATCAACGCAAAACCGGTAATCgg > 1:2760830/1‑69 (MQ=255) cATGATCATTTCATCCCGCCATGGTGAGTCCGAAAGCCAGGCATCAATCAACGCAAAACCGGTAATCgg > 1:1729880/1‑69 (MQ=255) cATGATCATTTCATCCCGCCATGGTGAGTCCGAAAGCCAGGCATCAATCAACGCAAAACCGGTAATCgg > 1:801663/1‑69 (MQ=255) cATGATCATTTCATCCCGCCATGGTGAGTCCGAAAGCCAGGCATCAATCAACGCAAAACCGGTAATCgg > 1:2917642/1‑69 (MQ=255) cATGATCATTTCATCCCGCCATGGTGAGTCCGAAAGCCAGGCATCAATCAACGCAAAACCGGTAATCgg > 1:1935004/1‑69 (MQ=255) cATGATCATTTCATCCCGCCATGGTGAGTCCGAAAGCCAGGCATCAATCAACGCAAAACCGGTAATCgg > 1:1512577/1‑69 (MQ=255) cATGATCATTTCATCCCGCCATGGTGAGTCCGAAAGCCAGGCATCAATCAACGCAAAACCGGTAATCgg > 1:1392215/1‑69 (MQ=255) cATGATCATTACATCCCGCCATGGTGAGTCCGAAAGCCAGGCATCAATCAACGCAAAACCGGTAATCgg > 1:639368/1‑69 (MQ=255) aTCATTTCATCCCGCCATGGTGAGTCCGAAAGCCAGGCATCAATCAACGCAAAACCGGTAATCGGTTTc < 1:586701/69‑1 (MQ=255) aTCATTTCATCCCGCCATGGTGAGTCCGAAAGCCAGGCATCAATCAACGCAAAACCGGTAATCGGTTTc > 1:67333/1‑69 (MQ=255) aTCATTTCATCCCGCCATGGTGAGTCCGAAAGCCAGGCATCAATCAACGCAAAACCGGTAATCGGTTTc < 1:3062136/69‑1 (MQ=255) aTCATTTCATCCCGCCATGGTGAGTCCGAAAGCCAGGCATCAATCAACGCAAAACCGGTAATCGGTTTc > 1:1523240/1‑69 (MQ=255) aTCATTTCATCCCGCCATGGTGAGTCCGAAAGCCAGGCATCAATCAACGCAAAACCGGTAATCGGTTTc > 1:3106104/1‑69 (MQ=255) aTCATTTCATCCCGCCATGGTGAGTCCGAAAGCCAGGCATCAATCAACGCAAAACCGGTAATCGGTTTc > 1:1157948/1‑69 (MQ=255) aTCATTTCATCCCGCCATGGTGAGTCCGAAAGCCAGGCATCAATCAACGCAAAACCGGTAATCGGTTTc > 1:859061/1‑69 (MQ=255) aTCATTTCATCCCGCCATGGTGAGTCCGAAAGCCAGGCATCAATCAACGCAAAACCGGTAATCGGTTTc < 1:2806729/69‑1 (MQ=255) aTCATTTCATCCCGCCATGGTGAGTCCGAAAGCCAGGCATCAATCAACGCAAAACCGGTAATCGGTTTc > 1:1266246/1‑69 (MQ=255) aTCATTTCATCCAGCCATGGTGAGTCCGAAAGCCAGGCATCAATCAACGCAAAACCGGTAATCGGTTTc > 1:2773909/1‑69 (MQ=255) | TCAGTTGTTGGCGCAACCGACAGGGTGATCACGGGCAATATCATATGGCGGATTGCGCTCATGATCATTTCATCCCGCCATGGTGAGTCCGAAAGCCAGGCATCAATCAACGCAAAACCGGTAATCGGTTTC > minE/893835‑893966 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |