| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 894,163 | C→T | 14.3% | A88T (GCG→ACG) | sapB ← | predicted antimicrobial peptide transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 894,163 | 0 | C | T | 14.3% | 136.3 / 9.2 | 76 | A88T (GCG→ACG) | sapB | predicted antimicrobial peptide transporter subunit |
| Reads supporting (aligned to +/- strand): ref base C (38/27); new base T (9/2); total (47/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.89e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01 | |||||||||||
ATCCTGCCACTTATGGCGCGTAATCCCGGCAATCATCCCCACGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGCCGGGAAGACCTCTTTTAACTGTTCGGCAATC > minE/894101‑894230 | aTCCTGCCACTTATGGCGCGTAATCCCGGCAATCATCCCCACGGGGATGCCAACAATCAGCGCaaacc > 1:2910308/1‑66 (MQ=255)aTCCTGCCACTTATGGCGCGTAATCCCGGCAATCATCCCCACGGGGATGCCAACAATCAGCGCaaaa > 1:2237765/1‑67 (MQ=255)aTCCTGCCACTTATGGCGCGTAATCCCGGCAATCATCCCCACGGGGATGCCAACAATCAGCGCaaa < 1:1075181/66‑1 (MQ=255)aTCCTGCCACTTATGGCGCGTAATCCCGGCAATCATCCCCACGGGGATGCCAACAATCAGCGCaaa < 1:1803912/66‑1 (MQ=255)aTCCTGCCACTTATGGCGCGTAATCCCGGCAATCATCCCCACGGGGATGCCAACAATCAGCGCaaa < 1:1286452/66‑1 (MQ=255)aTCCTGCCACTTATGGCGCGTAATCCCGGCAATCATCCCCACGGGGATGCCAACAATCAGCGCaaa < 1:2427301/66‑1 (MQ=255)aTCCTGCCACTTATGGCGCGTAATCCCGGCAATCATCCCCACGGGGATGCCAACAATCAGCGCaaa < 1:1402650/66‑1 (MQ=255)aTCCTGCCACTTATGGCGCGTAATCCCGGCAATCATCCCCACGGGGATGCCAACAATCAGCGCaaa < 1:78799/66‑1 (MQ=255)aTCCTGCCACTTATGGCGCGTAATCCCGGCAATCATCCCCACGGGGATGCCAACAATCAGCGCaaa < 1:74048/66‑1 (MQ=255)aTCCTGCCACTTATGGCGCGTAATCCCGGCAATCATCCCCACGGGGATGCCAACAATCAGCGCaaa < 1:3372469/66‑1 (MQ=255)aTCCTGCCACTTATGGCGCGTAATCCCGGCAATCATCCCCACGGGGATGCCAACAATCAGCGCaaa < 1:1517790/66‑1 (MQ=255)aTCCTGCCACTTATGGCGCGTAATCCCGGCAATCATCCCCACGGGGATGCCAACAATCAGCGCaaa < 1:2937663/66‑1 (MQ=255)aTCCTGCCACTTATGGCGCGTAATCCCGGCAATCATCCCCACGGGGATGCCAACAATCAGCGCAAAAc > 1:106047/1‑68 (MQ=255) cTTATGGCGCGTAATCCCGGCAATCATCCCCACGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAg < 1:1489899/69‑1 (MQ=255) cTTATGGCGCGTAATCCCGGCAATCATCCCCACGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAg < 1:834653/69‑1 (MQ=255) cTTATGGCGCGTAATCCCGGCAATCATCCCCACGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAg < 1:2891229/69‑1 (MQ=255) cTTATGGCGCGTAATCCCGGCAATCATCCCCACGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAg < 1:2892379/69‑1 (MQ=255) ggCGCGTAATCCCGGCAATCATCCCCACGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAGGATg < 1:43549/68‑1 (MQ=255) ggCGCGTAATCCCGGCAATCATCCCCACGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAGGATg < 1:2450244/68‑1 (MQ=255) ggCGCGTAATCCCGGCAATCATCCCCACGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAGGATg < 1:2817228/68‑1 (MQ=255) ggCGCGTAATCCCGGCAATCATCCCCACGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAGGATg < 1:368160/68‑1 (MQ=255) ggCGCGTAATCCCGGCAATCATCCCCACGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAGGATg < 1:230931/68‑1 (MQ=255) ggCGCGTAATCCCGGCAATCATCCCCACGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAGGATg < 1:2305762/68‑1 (MQ=255) ggCGCGTAATCCCGGCAATCATCCCCACGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAGGATg < 1:2099940/68‑1 (MQ=255) ggCGCGTAATCCCGGCAATCATCCCCACGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAGGATg < 1:641223/68‑1 (MQ=255) ggCGCGTAATCCCGGCAATCATCCCCACGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAGGATg < 1:1479444/68‑1 (MQ=255) ggCGCGTAATCCCGGCAATCATCCCCACGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAGGATg < 1:760377/68‑1 (MQ=255) ggCGCGTAATCCCGGCAATCATCCCCACGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAGGATg < 1:774114/68‑1 (MQ=255) gcgcGTAATCCCGGCAATCATCCCCACGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAGGATg < 1:814203/67‑1 (MQ=255) cccGGCAATCATCCCCACGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAGGATGCACAGTTCCa > 1:1565437/1‑68 (MQ=255) cccGGCAATCATCCCCACGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAGGATGCACAGTTCCAt > 1:1040751/1‑69 (MQ=255) cccGGCAATCATCCCCACGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAGGATGCACAGTTCCAt > 1:973453/1‑69 (MQ=255) cccGGCAATCATCCCCACGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAGGATGCACAGTTCCAt > 1:2859904/1‑69 (MQ=255) cccGGCAATCATCCCCACGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAGGATGCACAGTTCCAt > 1:3064458/1‑69 (MQ=255) cccGGCAATCATCCCCACGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAGGATGCACAGTTCCAt > 1:3378718/1‑69 (MQ=255) cccGGCAATCATCCCCACGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAGGATGCACAGTTCCAt > 1:1734385/1‑69 (MQ=255) cccGGCAATCATCCCCACGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAGGATGCACAGTTCCAt > 1:1790362/1‑69 (MQ=255) cccGGCAATCATCCCCACGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAGGATGCACAGTTCCAt > 1:2275305/1‑69 (MQ=255) catcTCCACGGGGATGCCAACAATCAGCGCAAAACC‑AAGGCGAg > 1:1025443/1‑44 (MQ=38) catcCCCACGGGGATGCCAACAATCAGCGCGAAACCGAAGGCGAg > 1:374517/1‑45 (MQ=255) catcCCCACGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAg > 1:3015428/1‑45 (MQ=255) catcCCCACGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAg > 1:28775/1‑45 (MQ=255) catcCCCACGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAg > 1:1547971/1‑45 (MQ=255) catcCCCACGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAg > 1:2527180/1‑45 (MQ=255) catcCCCACGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAGGATGCACAGTTCCATGGTCGCCg > 1:699542/1‑68 (MQ=255) catcCCCACGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGCCg > 1:1680610/1‑68 (MQ=255) catcCCCACGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGCCg > 1:1635906/1‑68 (MQ=255) catcCCCACGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGCCg > 1:821171/1‑68 (MQ=255) catcCCCACGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGCCg > 1:1312184/1‑68 (MQ=255) catcCCCACGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGCCg > 1:1415090/1‑68 (MQ=255) atcCCCACGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGCCg > 1:498958/1‑67 (MQ=255) atcCCCACGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGCCg > 1:1364549/1‑67 (MQ=255) atcCCCACGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGCCg > 1:408428/1‑67 (MQ=255) atcCCCACGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGCCg > 1:2352337/1‑67 (MQ=255) atcCCCACGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGCCg > 1:3299518/1‑67 (MQ=255) atcCCCACGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGCCg > 1:3127656/1‑67 (MQ=255) atcCCCACGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGCCg > 1:3062418/1‑67 (MQ=255) atcCCCACGGGGATGCCAACAATCAGCGCAAAACAGAAGGCGAGGATGCACAGTTCCATCGTCGCCg > 1:2736191/1‑67 (MQ=255) ccccACGGGGATGCCAACAATCAGCGTAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGCCGGGaa < 1:2886087/69‑1 (MQ=255) ccccACGGGGATGCCAACAATCAGCGTAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGCCGGGaa < 1:3148758/69‑1 (MQ=255) cccACGGGGATGCCAACAATCAGCGTAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGCCGGGAAg > 1:2616569/1‑69 (MQ=255) cccACGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGCCGGGAAg > 1:832594/1‑69 (MQ=255) cccACGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGCCGGGAAg > 1:1225864/1‑69 (MQ=255) cccACGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGCCGGGAAg > 1:2913564/1‑69 (MQ=255) cccACGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGCCGGGAAg > 1:60869/1‑69 (MQ=255) cccACGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGCCGGGAAg > 1:515161/1‑69 (MQ=255) cccACGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGCCGGGAAg > 1:2059988/1‑69 (MQ=255) cGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAGGATg < 1:2637222/41‑1 (MQ=255) aTGCCAACAATCAGCGTAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGCCGGGaa > 1:546310/1‑59 (MQ=255) aTGCCAACAATCAGCGTAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGCCGGGAAGATCTCtttt > 1:594484/1‑69 (MQ=255) aTGCCAACAATCAGCGTAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGCCGGGAAGACCTCtttt > 1:652643/1‑69 (MQ=255) aTGCCAACAATCAGCGTAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGCCGGGAAGACCTCtttt > 1:247236/1‑69 (MQ=255) aTGCCAACAATCAGCGTAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGCCGGGAAGACCTCtttt > 1:3363225/1‑69 (MQ=255) aTGCCAACAATCAGCGTAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGCCGGGAAGACCTCtttt > 1:973975/1‑69 (MQ=255) aTGCCAACAATCAGCGTAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGCCGGGAAGACCTCttt > 1:1748865/1‑68 (MQ=255) aTGCCAACAATCAGCGTAAAACCGAAGACGAGGATGCACAGTTCCATCGTCGCCGGGAAGACCTCtttt > 1:765167/1‑69 (MQ=255) gcAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGCCGGGAAGACCTCTTTTAACTGTTCGGCAATc > 1:1874474/1‑69 (MQ=255) | ATCCTGCCACTTATGGCGCGTAATCCCGGCAATCATCCCCACGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGCCGGGAAGACCTCTTTTAACTGTTCGGCAATC > minE/894101‑894230 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |