| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 894,957 | A→C | 35.5% | S370A (TCG→GCG) | sapA ← | predicted antimicrobial peptide transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 894,957 | 0 | A | C | 35.5% | 42.4 / 50.4 | 62 | S370A (TCG→GCG) | sapA | predicted antimicrobial peptide transporter subunit |
| Reads supporting (aligned to +/- strand): ref base A (11/29); new base C (12/10); total (23/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.40e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-01 | |||||||||||
TTCCACGCCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCA > minE/894896‑895015 | ttCCACGCCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAAc < 1:824611/69‑1 (MQ=255)ttCCACGCCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAAc < 1:3092184/69‑1 (MQ=255) gCCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTcg > 1:750606/1‑69 (MQ=255) gCCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTcg > 1:2249012/1‑69 (MQ=255) aaCGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGAt < 1:1260075/68‑1 (MQ=255) aaCGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGAt < 1:976226/68‑1 (MQ=255) aaCGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGAt < 1:768366/68‑1 (MQ=255) aaCGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGAt < 1:509672/68‑1 (MQ=255) aaCGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGAt < 1:2698626/68‑1 (MQ=255) aaCGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGAt < 1:2483690/68‑1 (MQ=255) aaCGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGAt < 1:2464586/68‑1 (MQ=255) aaCGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGAt < 1:2462326/68‑1 (MQ=255) aaCGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGAt < 1:2429697/68‑1 (MQ=255) aaCGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGAt < 1:2239100/68‑1 (MQ=255) aCGTGTGGGCACCCACAGTTTCAGCGTTAAGTTTTCCAGCGCCAACGCCTTCAACTGTTCGCGCGAt > 1:3290727/1‑67 (MQ=255) aCGTGTGGGCACCCACAGTTTCAGCGTTAAGTTTTCCAGCCCCACCGCCTTCAACTGTTCGCGCGAt > 1:545167/1‑67 (MQ=255) aCGTGTGGGCACCCACAGTTTCAGCGTTAAGTTTTCCAGCCCCAACGCCTTCAACTGTTCGCGCGAt > 1:968131/1‑67 (MQ=255) aCGTGTGGGCACCCACAGTTTCAGCGTTAAGTTTTCCAGCCCCAACGCCTTCAACTGTTCGCGCGAt > 1:1319065/1‑67 (MQ=255) aCGTGTGGGCACCCACAGTTTCAGCGTTAAGTTTTCCAGCCCCAACGCCTTCAACTGTTCGCGCGAt > 1:1372205/1‑67 (MQ=255) aCGTGTGGGCACCCACAGTTTCAGCGTTAAGTTTTCCAGCCCCAACGCCTTCAACTGTTCGCGCGAt > 1:3294333/1‑67 (MQ=255) aCGTGTGGGCACCCACAGTTTCAGCGTTAAGTTTTCCAGCCCCAACGCCTTCAACTGTTCGCGCGAt > 1:1386512/1‑67 (MQ=255) aCGTGTGGGCACCCACAGTTTCAGCGTTAAGTTTTCCAGCCCCAACGCCTTCAACTGTTCGCGCGAt > 1:368844/1‑67 (MQ=255) aCGTGTGGGCACCCACAGTTTCAGCGTTAAGTTTTCCAGCCCCAACGCCTTCAACTGTTCGCGCGAt > 1:597728/1‑67 (MQ=255) aCGTGTGGGCACCCACAGTTTCAGCGTTAAGTTTTCCAGCCCCAACGCCTT‑AACTGTTCGCGCGAt > 1:3037842/1‑66 (MQ=255) aCGTGTGGGCACCCACAGTTTCAGCGTTAAGTTTTACAGCCCCAACGCCTTCAACTGTTCGCGCGAt > 1:2002982/1‑67 (MQ=255) aCGTGTGGGCAACCACAGTTTCAGCGTTAAGTTTTCCAGCCCCAACGCCTTCAACTGTTCGCGCGAt > 1:2446437/1‑67 (MQ=255) cACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCACCGACTTCAACTGTTCGCGCGATTTCGCCGGAt > 1:3037316/1‑68 (MQ=255) cACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGAt > 1:1419130/1‑68 (MQ=255) cACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGAt > 1:3245585/1‑68 (MQ=255) cACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGAt > 1:1403401/1‑68 (MQ=255) cACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGAt > 1:1777709/1‑68 (MQ=255) aCCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTg < 1:449951/69‑1 (MQ=255) aCCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTg < 1:2821479/69‑1 (MQ=255) aCCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTg < 1:1814116/69‑1 (MQ=255) aCCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTg < 1:2290120/69‑1 (MQ=255) aCCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTg < 1:3239030/69‑1 (MQ=255) aCCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTg < 1:2288560/69‑1 (MQ=255) aCCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTg < 1:2059928/69‑1 (MQ=255) cacaGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTa > 1:673538/1‑68 (MQ=255) cacaGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTa > 1:2084434/1‑68 (MQ=255) tAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGc < 1:2262968/69‑1 (MQ=255) tAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGc < 1:2848284/69‑1 (MQ=255) tAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGc < 1:2493656/69‑1 (MQ=255) aaaTTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGcc > 1:3004349/1‑69 (MQ=255) aaaTTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGcc > 1:3425907/1‑69 (MQ=255) cAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGt < 1:1617620/68‑1 (MQ=255) gCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCa < 1:1050681/68‑1 (MQ=255) gCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCa < 1:3061292/68‑1 (MQ=255) gCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCa < 1:2419062/68‑1 (MQ=255) gCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCa < 1:516038/68‑1 (MQ=255) gCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCa < 1:160872/68‑1 (MQ=255) gCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTAGTTGTCa < 1:2049457/68‑1 (MQ=255) ccAACGCCTTCAACTGTTCGCGCGATTTTGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCa < 1:484323/65‑1 (MQ=255) ccAACGCCTTCAACTGTTCGCGCGATTTTGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCa < 1:2619602/65‑1 (MQ=255) ccAACGCCTTCAACTGTTCGCGCGATTTTGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCa < 1:376623/65‑1 (MQ=255) ccAACGCCTTCAACTGTTCGCGCGATTTTGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCa < 1:2790060/65‑1 (MQ=255) ccAACGCCTTCAACTGTTCGCGCGATTTTGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCa < 1:2476982/65‑1 (MQ=255) ccAACGCCTTCAACTGTTCGCGCGATTTTGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCa < 1:1644419/65‑1 (MQ=255) ccAACGCCTTCAACTGTTCGCGCGATTTTGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCa < 1:3317160/65‑1 (MQ=255) ccAACGCCTTCAACTGTTCGCGCGATTTTGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCa < 1:85309/65‑1 (MQ=255) ccAACGCCTTCAACTGTTCGCGCGATTTTGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCa < 1:2967740/65‑1 (MQ=255) aCGCCTTCAACTGTTCGCGTGATTTTGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCa < 1:29203/62‑1 (MQ=255) | TTCCACGCCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCA > minE/894896‑895015 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |