| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 899,437 | G→A | 100% | V35V (GTG→GTA) | pspD → | peripheral inner membrane phage‑shock protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 899,437 | 0 | G | A | 100.0% | 46.9 / NA | 21 | V35V (GTG→GTA) | pspD | peripheral inner membrane phage‑shock protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (14/7); total (14/7) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CTGGTACTTCTTACCGCACTGCGCTATGGCCCGGCGGGTGTGGCGGGCTGGGCGATAAAATCAGTTGCTCGCCGACCGCTGAAAA > minE/899396‑899480 | cTGGTACTTCTTACCGCGCTGCGCTATGGCCCTGCGGGTGTAGCTGGCTGGGCGAt > 1:2442629/1‑56 (MQ=255)cTGGTACTTCTTACCGCGCTGCGCTATGGCCCGGCGGGTGTAGCTGGCTGGGCGa > 1:2648118/1‑55 (MQ=255)cTGGTACTTCTTACCGCGCTGCGCTATGGCCCGGCGGGTGTAGCTGGCTGGGCGAt > 1:1778650/1‑56 (MQ=255)cTGGTACTTCTTACCGCGCTGCGCTATGGCCCGGCGGGTGTAGCTGGCTGGGCGAt > 1:521731/1‑56 (MQ=255)cTGGTACTTCTTACCGCGCTGCGCTATGGCCCGGCGGGTGTAGCTGGCTGGGCGAt > 1:484169/1‑56 (MQ=255)cTGGTACTTCTTACCGCGCTGCGCTATGGCCCGGCGGGTGTAGCTGGCTGGGCGAt > 1:3395278/1‑56 (MQ=255)cTGGTACTTCTTACCGCGCTGCGCTATGGCCCGGCGGGTGTAGCTGGCTGGGCGAt > 1:3334688/1‑56 (MQ=255)cTGGTACTTCTTACCGCGCTGCGCTATGGCCCGGCGGGTGTAGCTGGCTGGGCGAt > 1:3203883/1‑56 (MQ=255)cTGGTACTTCTTACCGCGCTGCGCTATGGCCCGGCGGGTGTAGCTGGCTGGGCGAt > 1:3142316/1‑56 (MQ=255)cTGGTACTTCTTACCGCGCTGCGCTATGGCCCGGCGGGTGTAGCTGGCTGGGCGAt > 1:3031930/1‑56 (MQ=255)cTGGTACTTCTTACCGCGCTGCGCTATGGCCCGGCGGGTGTAGCTGGCTGGGCGAt > 1:2662477/1‑56 (MQ=255)cTGGTACTTCTTACCGCGCTGCGCTATGGCCCGGCGGGTGTAGCTGGCTGGGCGAt > 1:2339670/1‑56 (MQ=255)cTGGTACTTCTTACCGCGCTGCGCTATGGCCCGGCGGGTGTAGCTGGCTGGGCGAt > 1:2076994/1‑56 (MQ=255)cTGGTACTTCTTACCGCGCTGCGCTATGGCCCGGCGGGTGTAGCTGGCTGGGCGAt > 1:2033711/1‑56 (MQ=255) cgcTGCGCTATGGCCCGGCGGGTGTAGCTGGCTGGGCGATAAAATCAGTTGCTCGCCGACCACTGaaaa < 1:2462752/67‑1 (MQ=255) cgcTGCGCTATGGCCCGGCGGGTGTAGCTGGCTGGGCGATAAAATCAGTTGCTCGCCGACCACTGaaaa < 1:3222870/67‑1 (MQ=255) cgcTGCGCTATGGCCCGGCGGGTGTAGCTGGCTGGGCGATAAAATCAGTTGCTCGCCGACCACTGaaaa < 1:447713/67‑1 (MQ=255) cgcTGCGCTATGGCCCGGCGGGTGTAGCTGGCTGGGCGATAAAATCAGTTGCTCGCCGACCACTGaaaa < 1:202589/67‑1 (MQ=255) cgcTGCGCTATGGCCCGGCGGGTGTAGCTGGCTGGGCGATAAAATCAGTTGCTCGCCGACCACTGaaaa < 1:1257172/67‑1 (MQ=255) cgcTGCGCTATGGCCCGGCGGGTGTAGCTGGCTGGGCGATAAAATCAGTTGCTCGCCGACCACTGaaaa < 1:884187/67‑1 (MQ=255) gcTGCGCTATGGCCCGGCGGGTGTAGCTGGCTGGGCGATAAAATCAGTTGCTCGCCGACCACTGaaaa < 1:3115890/67‑1 (MQ=255) | CTGGTACTTCTTACCGCACTGCGCTATGGCCCGGCGGGTGTGGCGGGCTGGGCGATAAAATCAGTTGCTCGCCGACCGCTGAAAA > minE/899396‑899480 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |