| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 900,712 | C→T | 44.1% | T186M (ACG→ATG) | ycjM → | predicted glucosyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 900,712 | 0 | C | T | 44.1% | 34.8 / 94.0 | 120 | T186M (ACG→ATG) | ycjM | predicted glucosyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (51/16); new base T (32/21); total (83/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.52e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01 | |||||||||||
Only 100 of 129 total aligned reads displayed.
CTCAGCGCCGTCACTCGCCCGCGTGCGTTACCGTTATTAACGCCATTCCAGATGCGCGATCATTCAACGCGCCATTTATGGACCACCTTTAGTGACGATCAAATTGACCTGAATTACCGTAGCCCTGAA > minE/900645‑900773 | ctcAGCGCCGTCACTCGCCCGCGTGCGTTACCGTTATTAACGCCATTCCAGATGCGCTATCATTCAAcg > 1:2822660/1‑69 (MQ=255)ctcAGCGCCGTCACTCGCCCGCGTGCGTTACCGTTATTAACGCCATTCCAGATGCGCGATCATTCAAcg > 1:2391261/1‑69 (MQ=255)ctcAGCGCCGTCACTCGCCCGCGTGCGTTACCGTTATTAACGCCATTCCAGATGCGCGATCATTCAAcg > 1:1529983/1‑69 (MQ=255)ctcAGCGCCGTCACTCGCCCGCGTGCGTTACCGTTATTAACGCCATTCCAGATGCGCGATCATTCAAcg > 1:2205857/1‑69 (MQ=255)ctcAGCGCCGTCACTCGCCCGCGTGCGTTACCGTTATTAACGCCATTCCAGATGCGCGATCATTCAAcg > 1:3223453/1‑69 (MQ=255)ctcAGCGCCGTCACTCGCCCGCGTGCGTTACCGTTATTAACGCCATTCCAGATGCGCGATCATTCAAcg > 1:2516188/1‑69 (MQ=255)ctcAGCGCCGTCACTCGCCCGCGTGCGTTACCGTTATTAACGCCATTCCAGATGCGCGATCATTCAAcg > 1:2854994/1‑69 (MQ=255)ctcAGCGCCGTCACTCGCCCGCGTGCGTTACCGTTATTAACGCCATTCCAGATGCGCGATCATTCAAcg > 1:1040106/1‑69 (MQ=255) ccGTCACTCGCCCGCGTGCGTTACCGTTATTAACGCCATTCCAGATGCGCGATCATTCAACGCGCCAtt < 1:710031/69‑1 (MQ=255) ccGTCACTCGCCCGCGTGCGTTACCGTTATTAACGCCATTCCAGATGCGCGATCATTCAACGCGCCAtt < 1:2841593/69‑1 (MQ=255) cGTCACTCGCCCGCGTGCGTTACCGTTATTAACGCCATTCCAGATGCGCGATCATTCAACGCGCCAtt < 1:1446217/68‑1 (MQ=255) cGTCACTCGCCCGCGTGCGTTACCGTTATTAACGCCATTCCAGATGCGCGATCATTCAACGCGCCAtt < 1:3392722/68‑1 (MQ=255) aCCGTTATTAACGCCATTCCAGATGCGCGATCATTCAATGCGCCATTTATGGACCACCTTTAGcgacga < 1:2706836/69‑1 (MQ=255) aCCGTTATTAACGCCATTCCAGATGCGCGATCATTCAATGCGCCATTTATGGACCACCTTTAGcgacga < 1:2504765/69‑1 (MQ=255) aCCGTTATTAACGCCATTCCAGATGCGCGATCATTCAATGCGCCATTTATGGACCACCTTTAGcgacga < 1:2071873/69‑1 (MQ=255) aCCGTTATTAACGCCATTCCAGATGCGCGATCATTCAATGCGCCATTTATGGACCACCTTTAGcgacga < 1:3344350/69‑1 (MQ=255) aCCGTTATTAACGCCATTCCAGATGCGCGATCATTCAATGCGCCATTTATGGACCACCTTTAGcgacga < 1:3424166/69‑1 (MQ=255) aCCGTTATTAACGCCATTCCAGATGCGCGATCATTCAATGCGCCATTTATGGACCACCTTTAGcgacga < 1:500922/69‑1 (MQ=255) aCCGTTATTAACGCCATTCCAGATGCGCGATCATTCAATGCGCCATTTATGGACCACCTTTAGcgacga < 1:690329/69‑1 (MQ=255) aCCGTTATTAACGCCATTCCAGATGCGCGATCATTCAATGCGCCATTTATGGACCACCTTTAGcgacga < 1:686581/69‑1 (MQ=255) aCCGTTATTAACGCCATTCCAGATGCGCGATCATTCAATGCGCCATTTATGGACCACCTTTAGcgacga < 1:1415179/69‑1 (MQ=255) aCCGTTATTAACGCCATTCCAGATGCGCGATCATTCAATGCGCCATTTATGGACCACCTTTAGcgacga < 1:1283986/69‑1 (MQ=255) aCCGTTATTAACGCCATTCCAGATGCGCGATCATTCAATGCGCCATTTATGGACCACCTTTAGcgacga < 1:1619616/69‑1 (MQ=255) aCCGTTATTAACGCCATTCCAGATGCGCGATCATTCAATGCGCCATTTATGGACCACCTTTAGcgacga < 1:854243/69‑1 (MQ=255) aCCGTTATTAACGCCATTCCAGATGCGCGATCATTCAACGCGCCATTTATGGACCACCTTTAGTGACg > 1:1331254/1‑68 (MQ=255) aCCGTTATTAACGCCATTCCAGATGCGCGATCATTCAACGCGCCATTTATGGACCACCTTTAGTGACg > 1:259106/1‑68 (MQ=255) aCCGTTATTAACGCCATTCCAGAT‑‑GCGATCATTCAACGCGCCATTTATGGACCACCTTTAGTGACGAt > 1:526271/1‑68 (MQ=255) aCCGTTATTAACGCCATCCCAGATGCGCGATCATTCAACGCGCCATTTATGGACCACCTTTAGTGACg > 1:2471502/1‑68 (MQ=255) ccGTTATTAACGCCATTCCAGATTCGCGATCATTCAATGCGCCATTTATGGACCACCTTTAGcgacga < 1:1399192/68‑1 (MQ=255) gTTATTAACGCCATTCCAGATGCGCGATCATTCAACGCGCCATTTATGGACCACCTTTATTGACGATCa > 1:2408430/1‑69 (MQ=255) gTTATTAACGCCATTCCAGATGCGCGATCATTCAACGCGCCATTTATGGACCACCTTTAGTGACGATCa > 1:1229569/1‑69 (MQ=255) gTTATTAACGCCATTCCAGATGCGCGATCATTCAACGCGCCATTTATGGACCACCTTTAGTGACGATCa > 1:2463628/1‑69 (MQ=255) gTTATTAACGCCATTCCAGATGCGCGATCATTCAACGCGCCATTTATGGACCACCTTTAGTGACGATCa > 1:2973594/1‑69 (MQ=255) gTTATTAACGCCATTCCAGATGCGCGATCATTCAACGCGCCATTTATGGACCACCTTTAGTGACGATCa > 1:1589496/1‑69 (MQ=255) gTTATTAACGCCATTCCAGATGCGCGATCATTCAACGCGCCATTTATGGACCACCTTTAGTGACGATCa > 1:2093027/1‑69 (MQ=255) attaACGCCATTCCAGATGCGCGATCATTCAACGCGCCATTTATGGACCACCTTTAGTGACGATCaaa > 1:3266761/1‑68 (MQ=255) aaCGCCATTCCAGATGCGCGATCATTCAATGCGCCATTTATGGACCACCTTTAGcgacga < 1:1718519/60‑1 (MQ=255) aaCGCCATTCCAGATGCGCGATCATTCAACGCGCCATTTATGGac > 1:3429198/1‑45 (MQ=255) aaCGCCATTCCAGATGCGCGATCATTCAACGCGCCATTTATGGACCACCTTTAGTGACGATCAAATTGa > 1:62959/1‑69 (MQ=255) aaCGCCATTCCAGATGCGCGATCATTCAACGCGCCATTTATGGACCACCTTTAGTGACGATCAAATTGa > 1:3374152/1‑69 (MQ=255) aaCGCCATTCCAGATGCGCGATCATTCAACGCGCCATTTATGGACCACCTTTAGTGACGATCAAATTGa > 1:2884984/1‑69 (MQ=255) aaCGCCATTCCAGATGCGCGATCATTCAACGCGCCATTTATGGACCACCTTTAGTGACGATCAAATTGa > 1:3250941/1‑69 (MQ=255) aaCGCCATTCCAGATGCGCGATCATTCAACGCGCCATTTATGGACCACCTTTAGTGACGATCAAATTGa > 1:2135918/1‑69 (MQ=255) aaCGCCATTCCAGATGCGCGATCATTCAACGCGCCATTTATGGACCACCTTTAGTGACGATCAAATTGa > 1:625113/1‑69 (MQ=255) aaCGCCATTCCAGATGCGCGATCATTCAACGCGCCATTTATGGACCACCTTTAGTGACGATCAAATTGa > 1:2829154/1‑69 (MQ=255) ccATTCCAGATGCGCGATCATTCAACGCGCCATTTATGGACCACCTTTAGTGACGATCAAATTGACCTg < 1:358193/69‑1 (MQ=255) ccATTCCAGATGCGCGATCATTCAACGCGCCATTTATGGACCACCTTTAGTGACGATCAAATTGACCTg < 1:1999516/69‑1 (MQ=255) ccATTCCAGATGCGCGATCATTCAACGCGCCATTTATGGACCACCTTTAGTGACGATCAAATTGACCTg < 1:2983192/69‑1 (MQ=255) ccAGATGCGCGATCATTCAATGCGCCATTTATGGacc > 1:3291764/1‑37 (MQ=255) ccAGATGCGCGATCATTCAATGCGCCATTTATGGACCACCTTTAGCGACGATCa > 1:322179/1‑54 (MQ=255) ccAGATGCGCGATCATTCAATGCGCCATTTATGGACCACCTTTAGCGACGATCa > 1:609469/1‑54 (MQ=255) ccAGATGCGCGATCATTCAATGCGCCATTTATGGACCACCTTTAGCGACGATCa > 1:2891939/1‑54 (MQ=255) ccAGATGCGCGATCATTCAATGCGCCATTTATGGACCACCTTTAGCGACGATCa > 1:330581/1‑54 (MQ=255) ccAGATGCGCGATCATTCAATGCGCCATTTATGGACCACCTTTAGCGACGATCa > 1:3374321/1‑54 (MQ=255) ccAGATGCGCGATCATTCAATGCGCCATTTATGGACCACCTTTAGCGACGATCa > 1:899037/1‑54 (MQ=255) ccAGATGCGCGATCATTCAATGCGCCATTTATGGACCACCTTTAGCGACGATCa > 1:458881/1‑54 (MQ=255) ccAGATGCGCGATCATTCAATGCGCCATTTATGGACCACCTTTAGCGACGATCa > 1:48015/1‑54 (MQ=255) ccAGATGCGCGATCATTCAATGCGCCATTTATGGACCACCTTTAGCGACGATCa > 1:506013/1‑54 (MQ=255) ccAGATGCGCGATCATTCAATGCGCCATTTATGGACCACCTTTAGCGACGATCa > 1:865417/1‑54 (MQ=255) ccAGATGCGCGATCATTCAATGCGCCATTTATGGACCACCTTTAGCGACGATCa > 1:1852182/1‑54 (MQ=255) ccAGATGCGCGATCATTCAATGCGCCATTTATGGACCACCTTTAGCGACGATCa > 1:217509/1‑54 (MQ=255) ccAGATGCGCGATCATTCAATGCGCCATTTATGGACCACCTTTAGCGACGATCa > 1:2184543/1‑54 (MQ=255) ccAGATGCGCGATCATTCAATGCGCCATTTATGGACCACCTTTAGCGACGATCa > 1:2007800/1‑54 (MQ=255) ccAGATGCGCGATCATTCAATGCGCCATTTATGGACCACCTTTAGCGACGATCa > 1:2242801/1‑54 (MQ=255) ccAGATGCGCGATCATTCAATGCGCCATTTATGGACCACCTTTAGCGACGATCa > 1:2374294/1‑54 (MQ=255) ccAGATGCGCGATCATTCAATGCGCCATTTATGGACCACCTTTAGCGACGATCa > 1:1806082/1‑54 (MQ=255) ccAGATGCGCGATCATTCAATGCGCCATTTATGGACCACCTTTAGCGACGATCa > 1:2467332/1‑54 (MQ=255) ccAGATGCGCGATCATTCAATGCGCCATTTATGGACCACCTTTAGCGACGATCa > 1:1674123/1‑54 (MQ=255) ccAGATGCGCGATCATTCAATGCGCCATTTATGGACCACCTTTAGCGACGATCa > 1:1644342/1‑54 (MQ=255) ccAGATGCGCGATCATTCAATGCGCCATTTATGGACCACCTTTAGCGACGATCa > 1:1127841/1‑54 (MQ=255) ccAGATGCGCGATCATTCAATGCGCCATTTATGGACCACCTTTAGCGACGATCa > 1:1079317/1‑54 (MQ=255) ccAGATGCGCGATCATTCAATGCGCCATTTATGGACAACCTTTAGCGACGATCa > 1:2326041/1‑54 (MQ=255) ccAGATGCGCGATCATTCAACGCGCCATTTATGGACCACCTTTAGTGACTATCAAATTGACCTGAAtt > 1:1007462/1‑68 (MQ=255) ccAGATGCGCGATCATTCAACGCGCCATTTATGGACCACCTTTAGTGACGATCAAATTGACCTGAATTa > 1:606658/1‑69 (MQ=255) ccAGATGCGCGATCATTCAACGCGCCATTTATGGACCACCTTTAGTGACGATCAAATTGACCTGAATTa > 1:2092595/1‑69 (MQ=255) ccAGATGCGCGATCATTCAACGCGCCATTTATGGACCACCTTTAGTGACGATCAAATTGACCTGAATTa > 1:1909634/1‑69 (MQ=255) ccAGATGCGCGATCATTCAACGCGCCATTTATGGACCACCTTTAGTGACGATCAAATTGACCTGAATTa > 1:151115/1‑69 (MQ=255) ccAGATGCGCGATCATTCAACGCGCCATTTATGGACCACCTTTAGTGACGATCAAATTGACCTGAATTa > 1:457838/1‑69 (MQ=255) ccAGATGCGCGATCATTCAACGCGCCATTTATGGACCACCTTTAGTGACGATCAAATTGACCTGAATTa > 1:2020836/1‑69 (MQ=255) ccAGATGCGCGATCATTCAACGCGCCATTTATGGACCACCTTTAGTGACGATCAAATTGACCTGAATTa > 1:2749640/1‑69 (MQ=255) ccAGATGCGCGATCATTCAACGCGCCATTTATGGACCACCTTTAGTGACGATCAAATTGACCTGAATTa > 1:2950390/1‑69 (MQ=255) ccACATGCGCGATCATTCAATGCGCCATTTATGGACCACCTTTAGCGACGAta > 1:2569513/1‑52 (MQ=255) gATGCGCGATCATTCAATGCGCCATTTATGGACCACCTTTAGcgacga < 1:1901337/48‑1 (MQ=255) tGCGCGATCATTCAACGCGCCATTTATGGACCACCTTTAGTg > 1:2734518/1‑42 (MQ=255) tGCGCGATCATTCAACGCGCCATTTATGGACCACCTTTAGTGACGATCAAATTGACCTGAATTACCGTa > 1:2053553/1‑69 (MQ=255) tGCGCGATCATTCAACGCGCCATTTATGGACCACCTTTAGTGACGATCAAATTGACCTGAATTACCGTa > 1:1013490/1‑69 (MQ=255) tGCGCGATCATTCAACGCGCCATTTATGGACCACCTTTAGTGACGATCAAATTGACCTGAATTACCGTa > 1:2717592/1‑69 (MQ=255) tGCGCGATCATTCAACGCGCCATTTATGGACCACCTTTAGTGACGATCAAATTGACCTGAATTACCGTa > 1:1411426/1‑69 (MQ=255) tGCGCGATCATTCAACGCGCCATTTATGGACCACCTTTAGTGACGATCAAATTGACCTGAATTACCGTa > 1:1495363/1‑69 (MQ=255) tGCGCGATCATTCAACGCGCCATTTATGGACCACCTTTAGTGACGATCAAATTGACCTGAATTACCGTa > 1:2449776/1‑69 (MQ=255) tGCGCGATCATTCAACGCGCCATTTATGGACCACCTTTAGTGACGATCAAATTGACCTGAATTACCGTa > 1:1874460/1‑69 (MQ=255) tGCGCGATCATTCAACGCGCCATTTATGGACCACCTTTAGTGACGATCAAATTGACCTGAATTACCGTa > 1:2206332/1‑69 (MQ=255) aTCATTCAATGCGCCATTTATGGACCACCTTTAGcgacga < 1:3303070/40‑1 (MQ=38) tCATTCAACGCGCCATTTATGGACCACCTTTAGTGACGATCAAATTGACCTGAATTACCGTAGCCCTGa < 1:2902777/69‑1 (MQ=255) tCATTCAACGCGCCATTTATGGACCACCTTTAGTGACGATCAAATTGACCTGAATTACCGTAGCCCTGa < 1:2259481/69‑1 (MQ=255) tCATTCAACGCGCCATTTATGGACCACCTTTAGTGACGATCAAATTGACCTGAATTACCGTAGCCCTGa < 1:2220960/69‑1 (MQ=255) tCATTCAACGCGCCATTTATGGACCACCTTTAGTGACGATCAAATTGACCTGAATTACCGTAGCCCTGa < 1:2737136/69‑1 (MQ=255) tCATTCAACGCGCCATTTATGGAACACCTTTAGTGACGATCAAATTGACCTGAATTACCGTAGCCCTGa < 1:2391991/69‑1 (MQ=255) cATTCAACGCGCCATTTATGGACCACCTTTAGTGACGATCAAATTGACCTGAATTACCGTAGCCCTGaa > 1:435948/1‑69 (MQ=255) cATTCAACGCGCCATTTATGGACCACCTTTAGTGACGATCAAATTGACCTGAATTACCGTAGCCCTGa < 1:2626269/68‑1 (MQ=255) | CTCAGCGCCGTCACTCGCCCGCGTGCGTTACCGTTATTAACGCCATTCCAGATGCGCGATCATTCAACGCGCCATTTATGGACCACCTTTAGTGACGATCAAATTGACCTGAATTACCGTAGCCCTGAA > minE/900645‑900773 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |